• Nutritional Sciences
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• 제9권4호
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• pp.240-247
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• 2006

In this study we investigated the neuroprotective, antioxidative, and hypocholestrolemic effects of dietary soy protein and soy isoflavone in a rat focal brain ischemia model. Weaning Sprague-Dawley rats were fed a 20% casein-based diet (CA), 20% soy protein-based diet (SP), or 0.2% soy isoflavones-supplemented diet (ISO) for 6 weeks. The cortical infarction volume of the ISO group was significantly lower than that of the SP group. The thiobarbituric acid reactive substances (TBARS) were considerably lower in the ISO group than the CA group. Glutatbione peroxidase activities of the SP group were notably higher than those of the CA group. Acetylcholinesterase (AchE) activities of the SP group were significantly decreased compared to the CA group. LDL cholesterol levels and LDL/HDL ratios of the ISO group were lower than those of the CA and SP groups. Our results collectively suggest that soy isoflavones may contribute to neuroprotection by reducing the TBARS and serum LDL/HDL ratio, whereas soy protein may be associated with the regulation of cognitive functions by modulating AchE activity.

• 한국식품과학회지
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• 제28권1호
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• pp.53-57
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• 1996

최근에 난과 식물에 속하는 천마의 농가재배가 성공함에 따라 천마를 이용한 음용차의 개발 등이 추진되고 있다. 본 연구에서는 천마의 성분을 분석하였고 Sprague-Dawely (SD) rat을 이용하여 천마의 기능성을 조사하였다. 사용된 건조 천마는 수분 11.8, 조단백질 7.6, 회분 3.2, 조지방 0.5, 조섬유 3.9, 조탄수화물 72.9%(w/w) 로 분석되었다. 무기질 함량은 칼슘 121, 나트륨 83, 철 6.2, 인 170, 마그네슘 69, 칼륨 1,278 mg% 수준 이었다. SD rat에 분말 천마가 0, 0.15, 1.5, 5.0% 첨가된 chow diet를 4주간 섭식시키며 실험한 결과 실험군간에 몸무게의 유의적인 차이는 없었으며 혈중 콜레스테롤은 5% 섭취군이 49.3 mg/dl로서 대조군 (63.7 mg/dl)에 비하여 현저히 저하되었다. 0.15% 첨가군은 가장 높은 진정 효과를 나타내었다. 천마 식이는 본 연구에서 조사된 대장의 상재 균총조성에는 큰 영향이 없었고 대장 내용물의 ${\beta}-glucuronidase$ 활성 수준도 유의적 변화가 나타나지 않았다. ${\beta}-Glucosidase$의 경우에는 5%첨가군이 대조군에 비하여 유의적으로 높았다.

• 한국식품영양과학회지
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• 제36권4호
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• pp.400-404
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• 2007

흰쥐 우측요부에 RK3E-ras cell주입으로 7일 이내에 종양이 발달하는 것을 관찰하고, 단삼 추출물을 제조하여 항종양 효과를 관찰하고자 1주 후부터 2주 동안 육종 부위에 투여 후 종양의 크기와 무게를 측정하고 조직학적인 관찰을 통하여 암세포의 발달과 암전이 유무를 살펴보았다. 암종의 크기는 대조군에 비하여 단삼 추출물을 투여한 실험군에서 현저히 감소(p<0.01)하였으며, 암종의 중량 또한 실험군에서 현저히 감소(p<0.01)하였다. 조직학적 관찰 결과 종양을 둘러싸고 있는 섬유막은 대조군에 비하여 실험군에서 발달 해 있었으며, 암세포의 밀도는 실험군에 비하여 대조군에서 높았다. 간조직을 관찰한 결과 대조군의 간문맥 주변에서 전이된 것으로 보이는 암세포들이 다수 관찰되었다. 이와 같은 결과를 토대로 단삼 추출물이 항종양효과가 있다고 사료된다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.339-339
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• 1994

It has been reported that Indole-3-carbinol (I3C), a naturally occurring compound In cruclferous vegetables, exerts anticarcinogenic activity In several organs In rodents. The modifying effects of I3C were therefore assessed uging a rat multi-organ carcinogenesis model. A total of 100 male Sprague-Dawley rats were divided Into 3 groups. Animals of groups 1 and 2 were sequentially treated with diethylnitrosamine (DEN; 100 mg/kg b.w., i.p.), N-methylnitrosourea (NNU; 20 mg/kg b.w., 4 times for 2 weeks, i.p), and dihydroxy-di-N-propylnitrosauine (DHPN; 0.1% In d.w. for 2 weeks) for 4 weeks (DMD treatment). Animals of groups 1 and 3 were given the diet of 0.25% I3C for 20 weeks after DMD initiation and then were given basal diet for 28 weeks. All animals were sacrificed at week 24 and 52, respectively. I3C has been clearly demonstrated promoting effects on the development of glutathione S-transferase placental form (GST-P) positive hepatic foci at 24 weeks of the experiment. And I3C also exerted promoting potential In the hepatocellular adenoma (4/14; 29%) and the adenoma (7/14; 50%) of the thyroid gland at 52 weeks of the experiment. Therefore, I3C may promote hepatocarcinogenesis and thyroid tumorigenesis in the rat multi-organ carcinogenesis model.

• The Korean Journal of Pain
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• 제34권4호
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• pp.394-404
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• 2021

Background: We aimed to investigate the effect of epidural polydeoxyribonucleotide (PDRN) on mechanical allodynia and motor dysfunction in a rat model of lumbar foraminal stenosis (LFS). Methods: This study was conducted in two stages, using male Sprague-Dawley rats. The rats were randomly divided into eight groups. In the first stage, the groups were as follows: vehicle (V), sham (S), and epidural PDRN at 5 (P5), 8 (P8), and 10 (P10) mg/kg; and in the second stage, they were as follows: intraperitoneal PDRN 8 mg/kg, epidural 3,7-dimethyl-1-propargilxanthine (DMPX) (0.1 mg/kg), and DMPX (0.1 mg/kg). The LFS model was established, except for the S group. After an epidural injection of the test solutions, von Frey and treadmill tests were conducted for 3 weeks. Subsequently, histopathologic examinations were conducted in the V, S, P5, and P10 groups. Results: A total of 65 rats were included. The P8 and P10 groups showed significant recovery from mechanical allodynia and motor dysfunction at all time points after drug administration compared to the V group. These effects were abolished by concomitant administration of DMPX. On histopathological examination, no epineurial inflammation or fibrosis was observed in the epidural PDRN groups. Conclusions: Epidural injection of PDRN significantly improves mechanical allodynia and motor dysfunction in a rat model of LFS, which is mediated by the spinal adenosine A_2A receptor. The present data support the need for further research to determine the role of epidural PDRN in spinal stenosis treatment.

• 대한약침학회지
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• 제13권1호
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• pp.87-92
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• 2010

Objectives:This study was performed to examine the side effect of inflammatory reactions of Pharmacopuncture lubricants (CF and JsD) using animal model. Methods:Pharmacopuncture lubricants (CF and JsD) were treated repeatedly the same point in Sprague-Dawley rats. And after finish the treatment, we obtain the tissue of muscle layer in rats where Pharmacopuncture lubricants were treated. And we observed the tissue how the change occurred in HE staining. Results: 1. There was no evidence to make inflammation induced by pharmacopuncture lubricants except 4 weeks treated group. 2. Only 4 weeks treated group(CF, JsD) were showed some infectional tissue's view in H-E staining. But it is not certain due to Pharmacopuncture lubricants. Conclusion:It appears that prolonged usage of pharmacopuncture lubricant on the specific point can induce inflammation, so we may be careful for treating time and point using pharmacopuncture lubricant in clinics.

• Journal of Nutrition and Health
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• 제28권8호
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• pp.717-726
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• 1995

This study was to investigate the validity of energy measurement system by using different body size and sex of rats and feed supplementation methods. There appeared no statistical differences in energy(AME, AMEn) utilization between male and female rats with average 200g body weight, and between male rat of 200g and 300g body weight. Glucose replacing corn in the basal diet or replacing diet itself at a level of 30% by weight appeared to have the same metabolizable energy value, suggesting that either methods to supplement test ingredient brought about the same results. Consequently, following suggestions could be made from results of present study : first, Sprague-Dawley rate of either sex weighing 200-300g may be used as an animal model to obtain in vivo metabolizable energy from dietary carbo-hydrates or sea foods. Second, the testing ingredients may be added at the expence of corn in the basal diet at 30% level.

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2002년도 Current Trends in Toxicological Sciences
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• pp.68-68
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• 2002

• 동의생리병리학회지
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• 제25권6호
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• pp.989-995
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• 2011

Hypothyroidism is the most common disease in the endocrine system. Cistanche Deserticola has been used traditionally in the treatment of kidney deficiency and Yang deficiency syndrome. In this study, we investigated the therapeutic effects of Cistanche Deserticola on Hypothyroidism rat model induced by PTU. 24 Two-month-old Sprague-Dawley male rats were divided into 4 groups : 1) normal(n=6), 2) PTU-induced hypothyroidism control(n=6), 3) hypothyroidism rat treated with Cistanche Deserticola(n=6), 4) hypothyroidism rat treated with levothyroxine(n=6). PTU was administered for 4 weeks. Cistanche Deserticola and levothyroxine was administered 2 weeks after PTU was initiated for a total duration of 2 week. Body weights were checked every week and after 4 weeks, biochemical analysis was performed and T3, T4 and TSH were measured by ELISA kits. In comparison with the normal group, the control group showed hypothyroidism with lower T3, T4 level and higher TSH level. In the Cistanche group the level of T4 was significantly increased and TSH level was significantly decreased. There was no significant difference in biochemical labs and weight between the Cistanche group and the control group. These findings suggest that Cistanche Deserticola could help the production of thyroid hormones under PTU suppression. And there is no harmful effect on liver and kidney function, and other metabolism. According to these results Cistanche Deserticola could be an useful agent for treating hypothyroidism.

• 대한한방내과학회지
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• 제30권2호
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• pp.327-337
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• 2009

Objective : In benign prostatic hyperplasia, dihydrotestosterone acts as a potent cellular androgen and promotes prostate growth. Inhibiting enzyme 5${\alpha}$-reductase, which is involved in the conversion of testosterone to the active form dihydrotestosterone, reduces excessive prostate growth. Recently Scutellaria baicalensis has been related reports about the effect of baicalein on anti-proliferation of the prostate gland. In this study, we investigated the effects of Scutellaria baicalensis on cytopathological alterations and expression of 5${\alpha}$-reductase in the rat model of benign prostatic hyperplasia induced by castration and testosterone treatment. Methods : Sprague-Dawley rat were treated with testosterone after castration for induction of experimental benign prostatic hyperplasia, which is similar to human benign prostatic hyperplasia in histopathological profiles. Scutellaria baicalensis as an experimental specimen, and finasteride as a positive control, were administered orally. The prostates were evaluated by histopathological changes, testosterone levels, and the expression of 5${\alpha}$-reductase genes. Results : While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation, the rats treated with Scutellaria baicalensis showed a diminished range of tissue damage. In the reverse transcription-polymerase chain reaction(RT-PCR) of 5${\alpha}$-reductase genes. Scutellaria baicalensis inhibited the expression of 5${\alpha}$-reductase genes. Conclusions : These findings suggest that Scutellaria baicalensis may protect glandular epithelial cells and also inhibit stromal proliferation in association with the suppression of 5${\alpha}$-reductase. From theses results, we suggest that Scutellaria baicalensis could be a useful remedy agent for treating benign prostatic hyperplasia.