• 한국미생물·생명공학회지
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• 제50권3호
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• pp.436-440
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• 2022

본 연구에서는 염색체가공기술을 이용하여 xylan으로부터 다양한 대사산물을 생산할 수 있는 유전자를 도입한 효모인 공염색체를 구축하였다. 효율적인 염색체가공기술인 PCS법을 이용하기 위해 염색체 splitting에 필요한 splitting fragment (DNA module)를 각각 제작하였고, xylan 대사에 관여하는 유전자군을 가진 YKY164 균주에 형질전환하였다. 두번의 염색체 splitting에 의해 1,124 kb의 효모 7번염색체는 887 kb-YAC, 45 kb-mini YAC와 198 kb-YAC로 가공되었으며, 총 18개의 염색체를 가진 YKY183 균주를 구축하였다. 염색체가공을 위한 splitting efficiency는 50-78%였으며, 45 kb-mini YAC 상에 있는 외래유전자의 발현 및 효소활성은 염색체가공 전과 비교하여 유의미한 변화 및 저하는 관찰되지 않았다. 또한 생산된 재조합효소에 의한 xylan의 분해산물을 확인하였으며, 160 generation 동안 미니 효모인 공염색체는 염색체의 결실없이 안정적인 mitotic stability를 유지하였다.

• Journal of the Korean Data and Information Science Society
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• 제13권1호
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• pp.97-104
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• 2002

Regression trees, a technique in data mining, are constructed by splitting function-a independent variable and its threshold. Lee (2002) considered one-sided purity (OSP) and one-sided extreme (OSE) splitting criteria for finding a interesting node as early as possible. But these methods cannot be crossed each other in the same tree. They are just concentrated on OSP or OSE separately in advance. In this paper, a new splitting method, which is the combination and extension of OSP and OSE, is proposed. By these combined criteria, we can select the nodes by considering both pure and extreme in the same tree. These criteria are not the generalized one of the previous criteria but another option depending on the circumstance.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권6호
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• pp.590-594
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• 2006

Dental implant has become one of the important option for completely or partially edentulous patients, But it is challenging to reconstruct the severely atrophic ridge. Insufficient bone volume could restrict to place the wide and long implant and because of excessive interocclusal clearance, improper prosthetics could be produced. In this case bone augmentation for implant dentistry is necessary procedure to improve the insufficient bone volume. Therefore, bone augmentation or GBR is the most important procedure for successful implant placement and for ideal crown- root ratio. There are various bone augmentation techniques have been introduced recently; like block bone graft, distraction osteogenesis, inlay graft, onlay graft, etc.... In severe bucco-lingual resorption area, ridge splitting is the first choice of the treatment, because it provides a place for implantation and also has compaction effect. This technique may be indicated for sharp mandible and maxillary ridges in patients whose bone quantity is inadequate for primary stabilization. We report that the clinical experience of bone augmentation using ridge splitting technique in bucco-lingual bone resorption area.

• 한국공작기계학회논문집
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• 제16권1호
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• pp.9-18
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• 2007

There have been continuous efforts for automation of joint tracking system. This automation process is mainly used to do in root pass of gas metal arc welding in the field of heavy industry and shipbuilding etc. For automation, it is important using of vision sensor. Welding robot with vision sensor is used for weld seam tracking on welding fabrication. Recently, it is used to on post-weld inspection for weld quality evaluation. For real time seam tracking, it is very important role in vision process technique. Vision process is included in filtering and thinning, segmentation processing, feature extraction and recognition. In this paper, it has shown performance comparison results of seam tracking for real time root pass on gas metal arc welding. It can be concluded better segment splitting method than iterative averaging technique in the performance results of seam tracking.

• 정보처리학회논문지D
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• 제11D권4호
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• pp.809-822
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• 2004

본 논문에서는 검색 위주의 공간 데이터베이스 시스템을 위한 R-tree 기반의 동시성 제어 기법을 제안한다. 기존의 제안된 기법들은 검색연산이 갱신연산과 동시에 수행되는 것을 막기 위해 노드에 공유 락이나 래치를 획득함으로써 갱신연산으로 인한 검색연산의 블록킹을 막을 수 없다는 문제를 가지고 있으며, 또한 R-tree같은 다차원 색인의 경우 갱신연산의 락 획득은 여러 노드에 걸쳐 일어날 수 있으며, 노드 분할과 같은 경우 오랜 시간동안 락을 획득하고 있을 수도 있기 때문에 검색연산은 장시간 블록킹이 되어야 하는 문제를 갖는다. 따라서 본 논문에서는 이러한 문제를 해결하기 위하여 노드의 엔트리들을 링크드 리스트로 연결하는 방법을 사용하여 노드에 엔트리를 삽입하고 있는 중에도 락이나 래치를 획득하지 않고 검색연산을 할 수 있는 링크드 리스트 기반의 동시성 제어 기법과, 노드 분할이 진행 중에 있는 노드에도 검색연산이 락이나 래치를 획득하지 않고 노드를 탐색할 수 있는 버전 기반의 동시성 제어 기법을 제안한다.

• 방사성폐기물학회지
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• 제21권1호
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• pp.175-182
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• 2023

APro, a modularized process-based total system performance assessment framework, was developed at the Korea Atomic Energy Research Institute (KAERI) to simulate radionuclide transport considering coupled thermal-hydraulic-mechanical-chemical processes occurring in a geological disposal system. For reactive transport simulation considering geochemical reactions, COMSOL and PHREEQC are coupled with MATLAB in APro using an operator splitting scheme. Conventionally, coupling is performed within a MATLAB interface so that COMSOL stops the calculation to deliver the solution to PHREEQC and restarts to continue the simulation after receiving the solution from PHREEQC at every time step. This is inefficient when the solution is frequently interchanged because restarting the simulation in COMSOL requires an unnecessary setup process. To overcome this issue, a coupling scheme that calls PHREEQC inside COMSOL was developed. In this technique, PHREEQC is called through the "MATLAB function" feature, and PHREEQC results are updated using the COMSOL "Pointwise Constraint" feature. For the one-dimensional advection-reaction-dispersion problem, the proposed coupling technique was verified by comparison with the conventional coupling technique, and it improved the computation time for all test cases. Specifically, the more frequent the link between COMSOL and PHREEQC, the more pronounced was the performance improvement using the proposed technique.

• Journal of applied mathematics & informatics
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• 제24권1_2호
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• pp.17-30
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• 2007

A new scheme for solving the Vlasov equation using a compactly supported wavelets basis is proposed. We use a numerical method which minimizes the numerical diffusion and conserves a reasonable time computing cost. So we introduce a representation in a compactly supported wavelet of the derivative operator. This method makes easy and simple the computation of the coefficients of the matrix representing the operator. This allows us to solve the two equations which result from the splitting technique of the main Vlasov equation. Some numerical results are exposed using different numbers of wavelets.

• 생명과학회지
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• 제20권5호
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• pp.789-793
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• 2010

복잡한 진핵생물에서의 물리적 지도 작성이나 기능해석에 효모인공염색체(YAC)를 이용하기 위해서는 원하는 target region의 인공염색체화 및 single-copy인 YAC의 복제수를 늘이는 것이 요구된다. 본 연구에서는 YAC manipulation system에 복제수 증폭시스템(copy number amplification system)을 도입한 Simultaneous YAC Manipulation-Amplification (SYMA) system을 구축하였다. 식물염색체를 가진 YAC clone의 splitting과 증폭을 위해 conditional centromere와 thymidine kinase (TK) 유전자를 가진 pBGTK plasmid를 구축하였고, splitting fragment의 PCR을 위한 주형으로 사용하였다. 590 kb의 YAC clone은 splitting과 동시에 copy number amplification element를 가진 100 kb YAC와 490 kb YAC로 분리되었고, 100 kb YAC는 유도기질로 3 mg/ml sulfanilamide와 $50\;{\mu}g/ml$ methotrexate (S3/M50)의 첨가에 의해 14.4배로 그 복제수가 증가하였음을 확인할 수 있었다.

• Progress in Superconductivity
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• 제5권1호
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• pp.9-12
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• 2003

We observed the splitting of the fluxon-flow branches in the current-voltage characteristics of serially stacked intrinsic Josephson junctions (IJJs) formed in $Bi_2$$Sr_2$$CaCu_2$$O_{ 8＋x}$ single crystals in the long-junction limit. Stacks of IJJs were sandwiched between two Au electrodes deposited on the top and the bottom of the stack using the ‘double-side cleaving technique’. In all the samples studied, the branch splitting started occurring for a dense fluxon configuration around 2 T and became more distinct in a higher magnetic field range. This observation can be explained in terms of switching between different Josephson fluxon modes in resonance with the collective plasma oscillations induced by both inductive and capacitive coupling between stacked IJJs. This is the first detailed and quantitative identification of the coherent flux-flow modes in stacked..

• Archives of Reconstructive Microsurgery
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• 제7권2호
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• pp.88-94
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• 1998

Free vascularized fibular transplantation is one of the most useful living bone reconstructive procedure in microsurgical field. Concerning about donor site morbidity, the donor has minor problems of ankle stability and muscle power weakness and transient peroneal nerve symptoms. That problems can be minimized with longitudinal splitted osteotomy on the donor fibula if the bone defect in recipient site is not so large. Half splitted fibula with peroneal arterial pedicle which contains nutrient artery and periosteal vessels, grafted bone can survive with those vascular supplies. Authors underwent five cases of half splitted free vascularized fibular transplantation from 1985. There were no evidence of devascularization in all cases, we can minimized donor morbidity with leaving half fibula intact on donor site. The problem of that technique is technically demanding in longitudinal splitting of bone without damage to peroneal nutrient vessels and periosteal soft tissues which attached to the bone. Authors can propose longitudinal half fibular transplantation is one of modification in free vascularized bone transplantation that minimize donor defect.