• 식물분류학회지
• /
• 제53권1호
• /
• pp.32-37
• /
• 2023

Spiraea prunifolia f. simpliciflora Nakai is a perennial shrub widely used for horticultural and medicinal purposes. We simultaneously obtained the complete plastid genome (plastome) and nuclear ribosomal gene transcription units, 45S nuclear ribosomal DNA (nrDNA) and 5S nrDNA of S. prunifolia f. simpliciflora, using Illumina short-read data. The plastome is 155,984 bp in length with a canonical quadripartite structure consisting of 84,417 bp of a large single-copy region, 18,887 bp of a short single-copy region, and 26,340 bp of two inverted repeat regions. Overall, a total of 113 genes (79 protein-coding genes, 30 tRNAs, and four rRNAs) were annotated in the plastome. The 45S nrDNA transcription unit is 5,848 bp in length: 1,809 bp, 161 bp, and 3,397 bp for 18S, 5.8S, and 26S, respectively, and 261 bp and 220 bp for internal transcribed spacer (ITS) 1 and ITS 2 regions, respectively. The 5S nrDNA unit is 512 bp, including 121 bp of 5S rRNA and 391 bp of intergenic spacer regions. Phylogenetic analyses showed that the genus Spiraea was monophyletic and sister to the clade of Sibiraea angustata, Petrophytum caespitosum and Kelseya uniflora. Within the genus Spiraea, the sections Calospira and Spiraea were monophyletic, but the sect. Glomerati was nested within the sect. Chamaedryon. In the sect. Glomerati, S. prunifolia f. simpliciflora formed a subclade with S. media, and the subclade was sister to S. thunbergii and S. mongolica. The close relationship between S. prunifolia f. simpliciflora and S. media was also supported by the nrDNA phylogeny, indicating that the plastome and nrDNA sequences assembled in this study belong to the genus Spiraea. The newly reported complete plastome and nrDNA transcription unit sequences of S. prunifolia f. simpliciflora provide useful information for further phylogenetic and evolutionary studies of the genus Spiraea, as well as the family Rosaceae.

• 생명과학회지
• /
• 제19권9호
• /
• pp.1183-1189
• /
• 2009

조팝나무는 목본이며 약용으로 매우 중요하며 우리나라 산림청 지정 보호수종이다. 이 속내 7집단에서 85개체에 대해 ISSR (inter simple sequence repeats) 마커로 이들 집단에 대한 유전적 변이와 집단구조를 조사하였다. 65개의 다형성 좌위와 78개 ISSR 유전자형을 얻었다. 덕유산 집단과 능동산 집단에는 1개체 이상 공유하는 유전자형이 포함되어 있었다. 전체 유전적 다양도는 종수준과 집단수준에서 각각 0.293과 0.183이였다. 집단의 분화($G_{ST}$)는 0.373으로 나타났다. 따라서 전체 변이의 37.3%는 집단 간에 있었다. ISSR 마커로 한국 내 조팝나무 집단의 분화는 잘 분리되어 ISSR로 조팝나무 집단 연구에 유익하며 유전적 다양도와 집단구조의 통찰은 종보전에 대한 기초 정보로 활용할 수 있을 것으로 사료된다.

• 생약학회지
• /
• 제27권4호
• /
• pp.389-396
• /
• 1996

Spiraea prunifolia var. simpliciflora (Rosaceae) is a deciduous. latifoliate shrub growing in most parts of Korea. The roots of this plant have been used for malaria, as antipyretics and emetics. From the roots of this plant, sterol glycoside and two triterpenoids were isolated and the structures were elucidated by chemical and spectroscopic methods. They were identified as $3{\beta}-hydroxyurs-12-ene-28-oic$ acid (ursolic acid.1), $2{\alpha}$, $3{\beta}$, $19{\alpha}-trihy-droxyurs-12-ene-28-oic$ acid (tormentic acid.2) and ${\beta}-sitoste-rol-3-O-{\beta}-D-glucopyranoside$ (3).

• 생약학회지
• /
• 제13권1호
• /
• pp.39-42
• /
• 1982

Spiraea prunifolia Sieb. et. Zucc. var. simpliciflora Nakai (Rosaceae) is distributed in Korea, and used as a folk medicine for antipyretic, antimalarial and emetic. Sterols were obtained from the methanolic extract of the root of above plant. The composition of sterols are campesterol and ${\beta}-sitosterol$ which has been determined by gaschromatographic analysis.

• 한국조경학회지
• /
• 제26권2호
• /
• pp.91-100
• /
• 1998

This paper is to study on rating system of some shrubs for pedestrian control with concentrate upon the density of branch. It was usd that Hibiscus syricacus L., Spiraea prunifolia var. simpliciflora Nak, Ligustrum obtusifolium S. et Z., Callicarpa dichotoma Raeusch., Rhoododendron mucrionulatum Turcz., Syringa vulgaris L., Weigela subsessilis L. H. Bailey, Cercis chinensis bunge, Forsythia koreana Nak., Euonymus alatus Sieb, Chaenomeles speciosa Nak., orbaria sorbifolia var. stellipila Max., Deutzia parviflora Bunge, Kerria japonica De Candolle, Prunus tomentosa Thunberg ex Murray, Purunus grandulosa for. albiplena Koehne. Shrubs are invesitgated ito the density of branch, the power of sprout, height, a rate of growth, hardness of naturalizaton, crown width and existence of thorns. Shrubs belonged to high group of rating system for pedestrian control were Euonymus alatus Sieb, Purunus grandulosa for. albiplena Koehne, Chaenomeles speciosa Nak., Spiraea prunifolia var. simpliciflora Nak., Prunus tomentosa Thunberg ex Murray, Rhododendron mucronulatum Turcz., Hibiscus syricacus L., Ligustrum obtusifolium S. et Z., Syringa vulgaris L., Weigela subsessilis L.H.Bailey.

• 한국환경복원기술학회지
• /
• 제15권2호
• /
• pp.103-115
• /
• 2012

To investigate the attributes of seed germination of the plants for greening of river bank slope, low-temperature treatment, chemical treatment, plant hormones treatment, and stratification were conducted using the seeds of 6 herbs growing naturally around river banks, such as Setaria glauca and faberii, Imperata cylindrica var. koenigii, Miscanthus sinensis, Pennisetum alopecuroides, Lespedeza cuneata and the seeds of 3 shrubs, such as Lespedeza bicolor., Rosa multiflora, Spiraea prunifolia for. simpliciflora. The findings are as follows. In case of herbs, Setaria glauca and faberii. have stone seeds. In the treatment with $H_2SO_4$, they were germinated through dormancy breaking, even though there were some differences depending on immersion time, and the germination rate of Setaria faberii was a bit higher than that of Setaria glauca. The germination rate of Imperata cylindrica var. koenigii was low in the treatment with $GA_3$, NAA, and the plant hormone, but the seed germination rate has improved and the germination rate of Miscanthus sinensis and Pennisetum alopecuroides showed the improved seed germination rate in low temperature treatment. In case of Lespedeza cuneata and bicolor, the leguminous plants, the treatment with $H_2SO_4$ was most effective for the improvement of seed germination and the decrease in germination time. Only Spiraea prunifolia for. simpliciflora, the rose among 3 woody plants showed the seed germination rate above 50% without any additional treatment. Thus, it was thought to be appropriate for greening. Rosa multiflora showed the attribute of germination in a dark condition at low temperature through stratification, despite its low germination rate. Based on the results as above, with respect to the greening using directly collected seeds, Miscanthus sinensis, Pennisetum alopecuroides, and Spiraea prunifolia for. simpliciflora are suitable for greening, because they do not need any additional treatment, and Lespedeza cuneata and bicolor are also suitable for greening because it is possible to improve the germination rate and reduce the germination time through the treatment on the seeds before sowing.

• Journal of Applied Biological Chemistry
• /
• 제61권4호
• /
• pp.397-403
• /
• 2018

조팝나무(Spiraea prunifolia) 잎을 80% MeOH 수용액으로 추출한 뒤, 감압 농축한 추출물을 EtOAc, n-BuOH과 $H_2O$층으로 계통 분획을 실시하였다. n-BuOH분획에 대하여 $SiO_2$, ODS column chromatograph 및 중압분취 장비를 반복실시하여 2종의 phenolic glucoside화합물을 분리 및 정제하였다. NMR 및 Mass데이터를 해석하여, isosalicin (1)와 crenatin (2)로 구조 동정 하였다. 분리한 2종의 화합물에 대하여 UPLC-MS/MS 질량 분석기를 기반으로 다중반응검지 (MRM mode) 분석법을 이용하여 정량분석 하였다. 그 결과, crenatin은 9.53 mg/g 및 isosalicin은 0.65 mg/g이 조팝나무 잎 추출물에 함유된 것을 확인하였다. 조팝나무 잎에서 분리된 화합물이 $H_2O_2$에 의해 유발된 산화스트레스로부터 신경세포 보호효과를 검증한 결과, 신경모세포종인 SK-N-MC 세포에 대해서 화합물 1 및 2 (100 mM) 모두 세포 독성은 보이지 않았으며, 특히 화합물 2 (crenatin)는 $H_2O_2$처리에 따른 신경세포의 외형적 변화, 세포 손상, 세포 사멸을 억제하여 산화스트레스로부터 신경세포를 보호하는 효과가 있음을 확인하였다.

• 한국환경복원기술학회지
• /
• 제15권1호
• /
• pp.15-25
• /
• 2012

Arundinaria munsuensis population were classified into Lespedeza maximowiczii dominant population and Spiraea prunifolia for. simpliciflora dominant population in the habitat of Jiri Mt. In the study sites, soil organic matter, total nitrogen, available phosphorous, exchangeable K, exchangeable Ca, exchangeable Mg contained, cation exchange capacity, and soil pH were ranged from 14.52~16.13%, 0.57~0.59%, 5.57~10.24 (mg/kg), 0.44~0.49 ($cmol^+/kg$), 0.62~0.92 ($cmol^+/kg$), 0.31~0.32 ($cmol^+/kg$), 28.26~32.44 ($cmol^+/kg$) and 4.33~4.48 respectively. DCCA ordination analysis showed that Lespedeza maximowiczii dominant population dwells where pH, exchangeable Ca and exchangeable Mg content are high and Spiraea prunifolia for. simpliciflora dominant population is placed where CEC, total nitrogen and organic matter content are high.

• Journal of Nutrition and Health
• /
• 제55권1호
• /
• pp.59-69
• /
• 2022

본 연구실의 선행 연구에서 항산화 활성이 가장 높았던 조팝나무 H₂O-EtOH (40:60) 분획물(SP60)의 항염 효능을 확인하여 기능성 소재로의 개발 가능성을 알아보고자 하였다. 항염활성 측정을 위해 RAW 264.7 세포에서 먼저 WST assay를 실시하여 SP60이 125 ㎍/mL 농도까지 세포 생존율에 영향을 미치지 않는 것을 확인하였다. Raw264.7 세포에 LPS와 SP60을 동시에 처리하여 NO, TNF-α, IL-6, IL-1β 생성과 iNOS, COX-2, NF-κB 발현을 측정하였다. SP60은 NO, TNF-α, IL-6, IL-1β 생성량을 모두 유의하게 억제시켰으며 western blot으로 iNOS, COX-2의 발현과 NF-κB의 활성을 측정한 결과 SP60을 처리한 군에서 농도 의존적으로 그 발현량과 활성을 억제하였다. 따라서 조팝나무 분획물은 항염 효능을 가진 것으로 판단되며 항염 관련 기능성 식품 및 소재로의 개발 가능성을 확인하였다.

• 한국환경복원기술학회지
• /
• 제7권2호
• /
• pp.87-95
• /
• 2004

For the slope stability and revegetation of retaining wall, Eco-Stone was built beside a newly constructed road in August, 2002. Eco-Stone blocks were constructed in 4 different combinations of irrigation and soil types. Within the Eco-Stone, planted were 6 species such as Forsythia koreana, Rhododendron mucronulatum, Spiraea prunifolia var. simpliciflora, Rhododendron sp. Euonymus japonica, and Aster koraiensis. Shoot growth was greater on common soils than at a better soil treatment for Forsythia koreana(P<0.01) and Spiraea prunifolia var. simpliciflora(P<0.05), while there were no significant differences for the other species. Biomass increment of the planted species also did not show significant differences between irrigation types, except Rhododendron sp. and Aster koraiensis of which biomass was higher under irrigation than at no irrigation for common soils. Most of the planted individuals were alive, showing survival ratio of 90~97% with no significant differences among treatment blocks. These results imply that the Eco-Stone can be used economically for slope stability and revegetation instead of concrete blocks, without a specific soil and irrigation requirement.