• 대한의생명과학회지
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• 제10권4호
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• pp.361-366
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• 2004

The purpose of this study was to determine the effect of high voltage pulsed current (HVPC) stimulation on proliferative activities of basal keratinocytes by measured nucleolar organizer region (NOR) expression and thickness of spinous layer in aged rat skin. Fifty-one weeks old twelve male Sprague-Dawley rats (300∼350g) were divided into control and HVPC stimulation groups. Each animal's hair on the back were removed. The HVPC stimulation group received an negative monophasic twin peak pulsed current stimulation with 50 V, while the control group was given the same treatment without electricity. The rats were sacrificed after 3 weeks. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with hematoxyline-eosin and silver nitrate. The thickness of basal to granular layer of the epidennis were measured using a light microscope and computerized image analysis system. The number of argyrophilic nucleolar organizer region (AgNOR) were counted using a light microscope and computerized image analysis system and calculated as the mean number of AgNOR per nucleus in the basal keratinocyte. By using a Student's t-test, an increase in the thickness of basal-spinous layer (P<0.001) of epidermis can be observed in HVPC stimulation rats as compared with the control rats, whereas the thickness of the granular layer is not affected. A Student's t-test showed a significantly higher mean NOR number per nucleus of the basal keratinocyte in the HVPC stimulation rats than control rats (P<0.001). There was significantly positive correlation between the NOR number and the thickness of basal-spinous layer (r=0.80, P<0.05). These results suggest that the HVPC stimulation may increase the thickness of spinous layer in the epidennis due to increased proliferative activities of basal keratinocytes in epidennis in aged rat skin.

• Clinical Pain
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• 제19권2호
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• pp.138-141
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• 2020

Clay-shoveler's fractures are rare stress-type avulsion fractures of the spinous processes especially in sports. There have been two case reports that discussed clay-shoveler's fractures in golf. A 36-year-old beginner golfer presented with a pain in the back after practicing golf swing. No fractures were detected using cervical radiography; however, computed tomography (CT) and magnetic resonance imaging (MRI) revealed T2~T3 spinous process fractures. The patient was treated conservatively and his pain subsided. The mechanism of injury is speculated to that of clay-shoveler's fractures. Therefore, if a golfer suffers persistent pain in the cervicothoracic region, clay-shoveler's fracture is one possibility to consider.

• Journal of Korean Neurosurgical Society
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• 제51권1호
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• pp.54-58
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• 2012

We present the rare case of solitary xanthogranuloma in the upper cervical column mimicking a Brown-Sequard syndrome. A 29-year-old man complained with right hemiparesis and left hypoesthesia after a car accident. Computed tomography and magnetic resonance images revealed a lobulated homogenously well-enhancing mass in between posterior arch of the atlas (C1) and spinous process of the axis (C2) resulting in a marked spinal canal narrowing with cortical erosions. The patient was managed by complete resection of the tumor with partial laminectomy with lower half of C1 posterior arch and upper half of C2 spinous process. The authors advise complete removal of the xanthogranuloma and consideration as a differential diagnosis of lesions among upper cervical lesions.

• Applied Microscopy
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• 제27권4호
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• pp.347-355
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• 1997

These studies were performed to observe the morphological changes of synapses in the visual cortex of rat during early postnatal development. Specimens of the visual cortex were taken from rats (Sprague Dawley) at 1, 3, 7, 14 and 21 days of age, and prepared for silver impregnation and electron microscopy. The number of synapse and the length of postsynaptic thickening were increased progressively with age, especially 14 and 21 days. The number of dendritic spine was increased conspicuously on postnatal days 14-21. And asymmetic, curved and axo-spinous synapses were increased markedly at the same ages. The present findings suggest that spurt of synaptogenesis in the rat visual cortex occurs during early postnatal development, especially in second to 3rd week period and asymmetric and/or curved axo-spinous synapse is a matured form of synapse with advanced age.

• 대한치과의사협회지
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• 제10권10호
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• pp.654-658
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• 1972

The toplications of 50% nicotin paste in oral mucous membrane of male mice weigh-ingabout 30 gram, were executed for 10, 15 and 20 days. And the tissues were observed in HE and PAS stain. The findings observed are as follows; I. Hyperplastic changes were revealed in underlying connective tissue and spinous cell layer. There were moderate vasodilatations with out edema but PAS reactions were dictinctive. in 10th day of experiment. II. There was remarkable increment of mortality up to 40percent and weight loss, but microscopic findings were not significant differences on than 10th days, in 15th day of experiment. III. Cellular anisocytosis, hyperchromatism and degenerations were observed in spinous cell layer, and vascular engergement in connec tivetissues were observed. And the mortality was up to 70 percent in 20th day of experment.

• The Journal of Korean Physical Therapy
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• 제35권6호
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• pp.163-166
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• 2023

A 30-year-old male novice golfer was diagnosed with a clay-shoveler's fracture. During golf practice, he experienced persistent posterior neck and upper back pain for a month. Cervical radiographs and computed tomography revealed a series of sequential spinous process fractures from C7 to T3. The patient was prescribed analgesic medication and fitted with a cervical brace alongside extracorporeal shockwave therapy (ESWT) directed explicitly toward the upper back region, subsequently leading to a notable reduction in pain. Therefore, ESWT could be considered an additional method for pain management in patients with clay-shoveler's fractures.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2009년도 추계학술대회 논문집
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• pp.621-622
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• 2009

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권2호
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• pp.132-136
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• 2000

The cell surface glycoprotein CD44 is a kind of adhesion molecule, which binds hyaluronic acid, type I collagen and fibronectin. Although there have been numerous reports on the expression and the function of CD44 in lymphocytes and macrophages, very little is known about its distribution and definite role in epithelial tissue, especially in oral epithelial one. The present study was performed to investigate the distribution and expression of the CD44 in human gingiva and squamous cell carcinoma(SCC) arising in human gingiva. And the authors compared CD44 expression with histopathologic grade of SCC. The results were as follows: 1. The CD44 was strongly expressed in granular, spinous and basal layers of normal marginal and attached gingiva, in spinous and basal layers of normal sulcular gingiva, and in all epithelial layers of normal junctional gingiva. 2. In SCC of gingiva, the CD44 was expressed in all but one case. In most of the cases the CD44 was expressed at cell membrane and the degree of expression was relatively strong. 3. In low-grade SCC of gingiva, the CD44 was strongly expressed, especially at the basal and spinous layers of abundantly keratinized cancer nests. In high-grade SCC of gingiva, the CD44 expression tended to be weak but was strong at cells showing individual keratinization. This study suggest that the CD44 expression of normal and cancerous gingival epithelium is associated with the degree of proliferation and differentiation of epithelial cells.

• 한국수산과학회지
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• 제9권3호
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• pp.209-213
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• 1976

여러가지 반문형을 가진 기름종개는 우리나라의 어디에나 널리 분포하지만 횡반B형과횡반 C형은 영산강과 섬진강에서만 서식하고 있어 주목된다. 이 2형은 반문과 지리적 분포상 차이를 보이면서도 이들의 체장분포, 아가미뚜껑뒤의 친한 흑갈색의 반점, 골질반의 구조, 비늘의 형태는 동일하다. 그러나 반문, 2차성징, 체장분포 및 비늘의 형태상 특징은 본속 기지종과는 분명희 구별된다. 이것은 지리적 격리에 의하여 생긴 고유한 형태로서 원래 동일종 이었던 것이 오랜 세월이 지남에 따라 한때 연결되었으리라고 추측되는 영산강과 섬진강이 각각 독립되므로 양개체군간에 교배가 불가능하기 때문에 반문의 분화가 생긴 것으로 본다.

• Journal of Trauma and Injury
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• 제34권1호
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• pp.31-38
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• 2021

Purpose: Traumatic vertebral injuries have a prevalence of 4-5% at level I centers. Studies have demonstrated that isolated thoracolumbar transverse process fractures (iTPF) rarely require brace or surgical interventions. We hypothesized that similarly isolated thoracolumbar spinous process fractures (iSPF) would have less need for bracing and operative interventions than SPFs with associated vertebral body (VB) fractures (SPF+VB). We performed a similar analysis for iTPF compared to transverse process fractures associated with VB injury (TPF+VB). Methods: In this single-center, retrospective study from 2012 to 2016, patients were classified into iSPF, SPF+VB, iTPF, and TPF+VB groups. Data including the fracture pattern, neurologic deficits, and operative intervention were obtained. The primary outcome studied was the need for bracing and/or surgery. A statistical analysis was conducted. Results: Of 98 patients with spinous process fractures, 21 had iSPF and 77 had SPF+VB. No iSPF patients underwent surgery, whereas 24 (31.17%) SPF+VB patients did undergo surgery (p=0.012). In the iSPF group, three patients (15%) received braces only for comfort, whereas 37 (48.68%) of the SPF+VB group required bracing (p=0.058). Of 474 patients with transverse process fractures, 335 had iTPF and 139 had TPF+VB. No iTPF patients underwent surgery, whereas 28 (20.14%) TPF+VB patients did (p≤0.001). Of the iTPF patients, six (1.86%) were recommended to receive braces only for comfort, while 68 (50.75%) of the TPF+VB patients required bracing (p<0.001). Conclusions: No patients with iSPF or iTPF required surgical intervention, and bracing was recommended to patients in these groups for comfort only. It appears that these injures may be safely managed without interventions, calling into question the need for spine consultation.