• BMB Reports
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• v.40 no.5
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• pp.708-714
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• 2007

The two-component signal transduction, which typically consists of a histidine kinase and a response regulator, is used by bacterial cells to sense changes in their environment. Previously, the SphS-SphR histidine kinase and response regulator pair of phosphate sensing signal transduction has been identified in Synechocystis sp. PCC 6803. In addition, some response regulators in bacteria have been shown to be cross regulated by low molecular weight phosphorylated compounds in the absence of the cognate histidine kinase. The ability of an endogenous acetyl phosphate to phosphorylate the response regulator, SphR in the absence of the cognate histidine kinase, SphS was therefore tested in Synechocystis sp. PCC 6803. The mutant lacking functional SphS and acetate kinase showed no detectable alkaline phosphatase activity under phosphate-limiting growth conditions. The results suggested that the endogenous acetyl phosphate accumulated inside the mutants could not activate the SphR via phosphorylation. On the other hand, exogenous acetyl phosphate could allow the mutant lacking functional acetate kinase and phosphotransacetylase to grow under phosphate-limiting conditions suggesting the role of acetyl phosphate as an energy source. Reverse transcription PCR demonstrated that the transcripts of acetate kinase and phospho-transacetylase genes in Synechocystis sp. PCC 6803 is up-regulated in response to phosphate limitation suggesting the importance of these two enzymes for energy metabolism in Synechocystis cells

• International Journal of Naval Architecture and Ocean Engineering
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• v.9 no.4
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• pp.390-403
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• 2017

Smoothed Particle Hydrodynamics (SPH) method has a good adaptability for the simulation of free surface flow problems. There are two forms of SPH. One is weak compressible SPH and the other one is incompressible SPH (ISPH). Compared with the former one, ISPH method performs better in many cases. ISPH based on Rankine source solution can perform better than traditional ISPH, as it can use larger stepping length by avoiding the second order derivative in pressure Poisson equation. However, ISPH_R method needs to solve the sparse linear matrix for pressure Poisson equation, which is one of the most expensive parts during one time stepping calculation. Iterative methods are normally used for solving Poisson equation with large particle numbers. However, there are many iterative methods available and the question for using which one is still open. In this paper, three iterative methods, CGS, Bi-CGstab and GMRES are compared, which are suitable and typical for large unsymmetrical sparse matrix solutions. According to the numerical tests on different cases, still water test, dam breaking, violent tank sloshing, solitary wave slamming, the GMRES method is more efficient than CGS and Bi-CGstab for ISPH method.

• Journal of Korean Tunnelling and Underground Space Association
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• v.15 no.3
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• pp.345-356
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• 2013

Numerical simulation on rock cutting by a TBM disc cutter was carried out using SPH (Smoothed Particle Hydrodynamics) code. AUTODYN3D, a commercial software program based on finite element method, was used in this study. The three-dimensional geometry of a disc cutter and a rock specimen were modeled by Lagrange and SPH code respectively. The numerical simulation was carried out for Hwangdeung granite for 10 different cutting conditions. The results of the numerical simulation, i.e. the relation between cutter force and failure behavior, had a good agreement with those from LCM test. The cutter forces measured in the numerical simulation had 10% deviation from the LCM test results. Moreover, the optimum cutter spacing was almost identical with the experimental results. These results indicate that SPH code can be successfully used had applicability for simulation on rock cutting by a TBM disc cutter. However, further study on Lagrange-SPH coupled modelling would be necessary to reduce the computation time.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.2
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• pp.625-631
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• 2012

In order to investigate whether SKI-II could reverse drug resistance and its possible mechanisms, we treated SGC7901/DDP cells with SKI-II or SKI-II in combination with DDP. Then cell growth, apoptosis, micromorphological changes, and expression of SphK1, P-gp, NF-${\kappa}B$, Bcl-2 and Bax were assessed by MTT assay, flow cytometry, electron microscopy, immunocytochemistry and Western blot assay respectively. SGC7901/DDP cells were insensitive to cisplatin 2.5mg/L, but when pretreated with SKI-II, their proliferation was inhibited by cisplatin 2.5mg/L significantly, the inhibition rate increasing with time and dose. The apoptosis rate was also significantly elevated. Expression of SphK1 and P-gp was decreased significantly, Pearson correlation analysis showing significant correlation between the two (r=0.595, P<0.01). Expression of NF-${\kappa}B$ and Bcl-2 was decreased significantly,while that of Bax was increased, compared to the control group. There were significant correlations between SphK1 and NF-${\kappa}B$(r=0.723, P<0.01), NF-${\kappa}B$ and Bcl-2(r=0.768, P<0.01). All these data indicated that SKI-II could reverse drug resistance of SGC7901/DDP to cisplatin by down-regulating expression of P-gp and up-regulating apoptosis through down-regulation of SphK1. The increased apoptotic sensitivity of SGC7901/DDP to cisplatin was due to the decreasing proportion of Bcl-2/Bax via down-regulating NF-${\kappa}B$.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.1
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• pp.32.2-32.2
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• 2018

We present the structural parameters of ~ 910 dwarf elliptical-like galaxies in the local universe ($z{\lesssim}0.01$) derived from the r-band images of the Sloan Digital SKy Survey (SDSS). We examine the dependence of structural parameters on the morphological types (dS0, dE, dEbc, dSph, and dEblue) and the environment. There is not much difference in the structural parameters among the five subtypes but the mean surface brightness within the effective radius (<${\mu}e$>) of dSph galaxies is clearly different from that of other subtypes. The frequency of disk features such as spiral arm, bar, lens, and rings strongly depends on the morphology of dwarf elliptical-like galaxies with no disk features in dSph galaxies. The absence of disk features and the low surface brightness of dSph galaxies are thought to be closely related to their low mass which leads to different evolution from other subtypes of dwarf elliptical-like galaxies. Density Environments Using IMSNG.

• Annals of Surgical Treatment and Research
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• v.95 no.5
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• pp.233-239
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• 2018

Purpose: In Korea, the working-hour limitation regulation has been implemented in December 2017. We aimed to define the difference in operative experience of surgical residents before and after implementing this policy in 2 hospitals among 8 affiliated hospitals of the Catholic Medical Center where implemented the 80 working-hour limitation policy since March 2015. Methods: All the operation records were reviewed, and the number of resident-participated surgeries between March and August in 2002 and 2017 were compared. Operations performed or participated in by residents as first assistants were defined as resident participated surgery. Results: After 2 years from the initiation of the resident work-hour limitations, the number of resident participated surgery has slightly decreased in both hospitals (Yeouido St. Mary's Hospital [YSM]: 317 to 302, St. Paul Hospital [SPH]: 635 to 461). For each resident, changes were like followings: $0{\rightarrow}21$ cases for R1, $65{\rightarrow}72$ cases for R2, $83{\rightarrow}192$ cases for R3, and $169{\rightarrow}17$ cases for R4 in YSM. In SPH, number of resident participating surgery was changed like followings: $4{\rightarrow}32$ cases for R1, $222{\rightarrow}100$ cases for R2, $317{\rightarrow}300$ cases for R3, and $92{\rightarrow}29$ cases for R4. In both hospital, while, total number of resident participating oncologic surgery has been decreased, number of resident participating appendectomy has been far increased. Activity of each grade resident is different according to hospital. Conclusion: Although total number of resident participating surgery decreased, variable changes were observed in each grade of resident according to each type of surgery and different hospitals. It is believed that comparisons of experiences from more hospitals in the future would be helpful in establishing the guidelines for surgical experience requirement of residents in Korea.

• Journal of The Korean Astronomical Society
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• v.29 no.1
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• pp.19-30
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• 1996

We have examined consequences of strong tidal encounters between a neutron star and a normal star using SPH as a possible formation mechanism of isolated recycled pulsars in globular clusters. We have made a number of SPH simulations for close encounters between a main-sequence star of mass ranging from 0.2 to 0.7 $M_\bigodot$ represented by an n=3/2 poly trope and a neutron star represented by a point mass. The outcomes of the first encounters are found to be dependent only on the dimensionless parameter $\eta'{\equiv}(m/(m+ M))^{1/2}(\gamma_{min}/R_{MS})^{3/2}(m/M)^{{1/6)}$, where m and M are the mass of the main-sequence star and the neutron star, respectively, $\gamma_{min}$ the minimum separation between two stars, and $R_{MS}$ the size of the main-sequence star. The material from the (at least partially) disrupted star forms a disk around the neutron star. If all material in the disk is to be acctreted onto the neutron star's surface, the mass of the disk is enough to spin up the neutron star to spin period of 1 ms.

• Journal of The Korean Astronomical Society
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• v.50 no.4
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• pp.111-124
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• 2017

The environmental dependence of the morphology of dwarf galaxies in isolated satellite systems is analyzed to understand the origin of the dwarf galaxy morphology using the visually classified morphological types of 5836 local galaxies with $z{\leq}0.01$. We consider six sub-types of dwarf galaxies, dS0, dE, $dE_{bc}$, dSph, $dE_{blue}$, and dI, of which the first four sub-types are considered as early-type and the last two as late-type. The environmental parameters we consider are the projected distance from the host galaxy ($r_p$), local and global background densities, and the host morphology. The spatial distributions of dwarf satellites of early-type galaxies are much different from those of dwarf satellites of late-type galaxies, suggesting the host morphology combined with $r_p$ plays a decisive role on the morphology of the dwarf satellite galaxies. The local and global background densities play no significant role on the morphology of dwarfs in the satellite systems hosted by early-type galaxies. However, in the satellite system hosted by late-type galaxies, the global background densities of dE and dSph satellites are significantly different from those of $dE_{bc}$, $dE_{blue}$, and dI satellites. The blue-cored dwarf satellites ($dE_{bc}$) of early-type galaxies are likely to be located at $r_p$ > 0.3 Mpc to keep their cold gas from the ram pressure stripping by the hot corona of early-type galaxies. The spatial distribution of $dE_{bc}$ satellites of early-type galaxies and their global background densities suggest that their cold gas is intergalactic material accreted before they fall into the satellite systems.

• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.6
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• pp.743-751
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• 2017

To evaluate the structural integrity of the helicopter radome, we performed bird strike analysis using SPH (Smoothed Particle Hydrodynamics) technique. Since the SPH method is a meshfree method, there is no phenomenon such as mesh tangling and it is suitable to predict the dispersion behavior of debris and debris cloud generated by high-speed impact. In order to observe the scattering direction of fractured bolts, the analysis were performed under the condition that the fracture occurs at the proof load. As a result of bird strike analysis, there is no secondary damage as well as the damage due to, the dispersion behavior of the bird model, and the scattering of the fractured bolts and radome. From the additional analysis that were performed to determine the actual bolt fracture, only plastic deformation is predicted since the maximum stress of the bolt does not exceed the ultimate stress.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.26 no.5
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• pp.343-349
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• 2013

Crashworthy fuel cells have a great influence on improving the survivability of crews. Since 1960's, the US army has developed a detailed military specification, MIL-DTL-27422, defining the performance requirements for rotorcraft fuel cells. In the qualification tests required by MIL-DTL-27422, the crash impact test should be conducted to verify the crashworthiness of fuel cell. Success of the crash impact test means the improvement of survivability of crews by preventing post-crash fire. But, there is a big risk of failure due to huge external load in the crash impact test. Because the crash impact test itself takes a long-term preparation efforts together with costly fuel cell specimens, the failure of crash impact test can result in serious delay of a entire rotorcraft development. Thus, the numerical simulations of the crash impact test has been required at the early design stage to minimize the possibility of trial-and-error with full-scale fuel cells. Present study performs the numerical simulation using SPH(smoothed particle hydro-dynamic) method supported by a crash simulation software, LS-DYNA. Test condition of MIL-DTL-27422 is reflected on analysis and material data is acquired by specimen test of fuel cell material. As a result, the resulting equivalent stresses of fuel cell itself are calculated and vulnerable areas are also evaluated.