• Title/Summary/Keyword: SphK1

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Nucleotide Sequencing Analysis of a Gene Coding for 3-Isopropylmalate Dehydrogenase of Kluyveromyces fragilis

  • Hong, Soon-Duck;Kim, Jong-Guk;Lee, Dong-Sun;Woo, Ju-Hyung;Lee, Sang-Yong
    • Journal of Microbiology and Biotechnology
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    • v.3 no.2
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    • pp.91-94
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    • 1993
  • A 3-isopropylmalate dehydrogenase (3-IPMD) gene was cloned from Kluyveromyces fragilis. pJK104 could complement Escherichia coli leuB and Saccharomyces cerevisiae leu2 auxotrophs. The coding region was subcloned and the nucleotide sequence was determined. A 1.8 Kb EcoRI/SphI fragment of pJK104 subcloned in pUC18 could still complement the leuB mutation. An open reading frame of 1164 bp that corresponds to a polypeptide of 387 amino acids was found in the cloned fragment. The homology between the 3-isopropylmalate dehydrogenase of S. cerevisiae and that of K. fragilis was 68.13% in nucleotides.

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A Study of K-9 engine Proper Depot maintenance Cycle (K-9 자주포 엔진 적정 창정비주기 연구)

  • Seo, Seong-Cheol;Kim, Tae-Gyun;Song, Bang-Won
    • Journal of the military operations research society of Korea
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    • v.31 no.2
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    • pp.75-85
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    • 2005
  • Even though K-series combat equipment's engine depot maintenance cycle of ROK army is 10years In average, that of the K-9 Self Propelled(SP) howitzer which has been fielded since 99 $3{\sim}4$years causing limitations to effective equipment operations and combat-readiness. Therefore, the current K-9 self-propelled howitzer engine operation period of 1,500 hours, which is greatly shorter than other equipments, had to be verified. In order to find the optimum depot maintenance cycle, related field operation conditions were verified and opinions were collected, and also the background on current depot maintenance cycle setting was studied.

Laboratory-Acquired Infections with Hantavirus at a Research Unit of Medical School in Seoul, 1996 (의과대학 연구동에서 발생한 유행성출혈열 역학조사)

  • Cho, Soo-Hun;Kang, Dae-Hee;Kim, Suhng-Gwon;Kim, Ik-Sang;Hong, Sung-Tae;Ju, Young-Su
    • Journal of Preventive Medicine and Public Health
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    • v.32 no.3
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    • pp.269-275
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    • 1999
  • Background : In April 6, 1990, a male researcher who has worked at a research unit at the Basic Research Building of Seoul National University(SNU) College of Medicine admitted to SNU Hospital due to persistent fever. He was diagnosed serologically as hemorrhagic fever with renal syndrome(HFRS). Another female researcher in the same unit was also diagnosed as HFRS at the same hospital several days later. Epidemic investigation of HFRS was conducted to determine the magnitude of the problems since these two cases were strongly suspected to have laboratory-acquired infections of HFRS. Methods : All researchers and employees working at the Basic Research Building(BRB) of SNU College of Medicine as of April 1, 1996 were recruited for the study, information on symptoms of HFRS and history of contact to experimental animals were collected by self-administered questionnaires and serological tests among study subjects were also conducted by indirect immunofluorescent antibody(IFA) to hantavirus. The experimental animals were also serologically tested for infection with hantavirus by IFA. Results : Among 218 surveyed, six researchers and an animal caretaker had hantavirus antibodies above 1:20 in IFA titer. Five of seven sero-positive subjects had antibodies above 1:640 in IFA titer and had shown clinical symptoms compatible to HFRS during Jan. 1 to Apr, 20, 1996. The sero-positive persons had handled animals more frequently than sero-negative persons (OR, 19,68; 95% Cl, 1.11-350.40) and handling animals at the animal quarter at School of Public Health(SPH) had shown consistently higher risk to get infected with hantavirus irrespective of types of animals handled (OR, 4.90-6.37). Sero-positivity of rats of the aniamal quarter at BRB was 30-60%, whereas 80% of rats at SPH tested were shown sero-positivity. Conclusion: There was a epidemic of HFRS in research units of a medical school during the period from Jan. through Apr, Further investigation is needed to determine the extent and the mode of transmission of the laboratory-acquired infection with hantavirus in other research facilities.

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Hydrodynamic simulations in the Galactic Center : Tilted HI disk

  • Lee, Joowon;Kim, Sungsoo S.
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.2
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    • pp.40.3-41
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    • 2016
  • Previous HI survey data have shown that the central HI gas in the Milky Way that resides within ~1.5 kpc of the Galactic Centre (GC) is tilted by ${\sim}15^{\circ}$ with respect to the Galactic plane. Although several models, such as a tilted disk model, have been suggested to interpret the observed morphology of the HI layer, it is still unknown what causes and how it preserves its tilted structure. We study the behavior of a gas disk near the GC using an N-body / SPH code. Our galaxy model includes four components; nuclear bulge, bulge, disk and halo. We construct a HI model whose radius is 1.3 kpc, scale height is 100 pc and mass is $3.6{\times}10^6M_{\odot}$. We also assume that the gas disk is initially tilted $30^{\circ}$ with respect to the Galactic plane. Here we report our simulation results and discuss the evolution of the tilted gas disk.

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BCCOMICS: Baryon-Cold dark matter COsMological Initial Condition generator for Small-scale structures

  • Ahn, Kyungjin
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.2
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    • pp.35.3-36
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    • 2016
  • Density and velocity perturbations in scales most relevant for the first galaxy formation are strongly affected by large-scale density perturbations, velocity-divergence perturbations and the baryon-cold dark matter (CDM) streaming velocities. Even at redshifts as high as z~200, this mode-mode coupling imprints a significant impact on the small-scale perturbations, at the wavenumber k >${\sim}100Mpc^{-1}$, as was calculated in our recent work. This implies that cosmological initial conditions based on the usual linear theory is no longer valid in these scales. We present a new cosmological initial condition generator, BCCOMICS, which generates initial conditions for the cold dark matter (CDM) and baryons in scales most relevant for the first galaxy formation. BCCOMICS is based on the linear perturbation theory including the mode-mode coupling terms, and generates cosmological initial conditions for the SPH-basded code GADGET and the AMR-based code ENZO. We also present our preliminary result on the cosmic variance of the first galaxy formation, studied by using BCCOMICS.

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Effects of galaxy-galaxy encounters on galactic spin and central mass distribution

  • Hwang, Jeong-Sun;Park, Changbom
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.61.4-62
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    • 2018
  • We use smoothed particle hydrodynamics (SPH) models to study the evolution of galactic spin and the distribution of gas and young stars in the inner region of the galaxies through galaxy encounters. Specifically, we perform numerical simulations of interactions of a late- or an early-type galaxy with either a late- or an early-type galaxy with and without a gas halo at the closest approach distances of 25 and 50 kpc. We find that an early-type galaxy encountering a late-type galaxy have a higher galactic spin and more gas and young stars in the central region of the galaxy after the collision. We are analyzing the role of a gas halo on the changes of galactic spin and central mass distribution during various galaxy-galaxy encounters.

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Hydrodynamics Simulation of the Off-Axis Cluster Merger Abell 115

  • Lee, Wonki;Kim, Mincheol;Jee, M. James
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.60.3-61
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    • 2018
  • Abell 115 is a renowned cluster merger at z=0.197. It exhibits an asymmetric X-ray distribution with cometary tails and a megaparsec-sized radio relic stretching in the northeastern direction from the core of the northern cluster. Many observations have concluded that this cluster merger has a large impact parameter, but there has been no numerical analysis on the structure of Abell 115. In this study, we simulate Abell 115 with Gadget2 N-body/SPH code to reproduce the X-ray and weak lensing features of Abell 115. We find a new plausible merger scenario of Abell 115, wherein the northern cluster is currently in an outgoing phase. The predicted X-ray emission has a similar morphology to the observed tail of the northern cluster. However, in order to reproduce the observed line-of-sight velocity and projected distance while maintaining the two systems gravitationally bound, the system should possess a large projection angle, which makes the shock look considerably more diffused than the observed radio relic.

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Construction of an Escherichia-Pseudomonas Shuttle Vector Containing an Aminoglycoside Phosphotransferase Gene and a lacZ' Gene for $\alpha$-Complementation

  • Lee, Bheong-Uk;Hong, Ja-Heon;Kahng, Hyung-Yeel;Oh, Kye-Heon
    • Journal of Microbiology
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    • v.44 no.6
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    • pp.671-673
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    • 2006
  • A new 4.87 kb Escherichia-Pseudomonas shuttle vector has been constructed by inserting a 1.27 kb DNA fragment with a replication origin of a Pseudomonas plasmid pRO1614 into the 3.6 kb E. coli plasmid pBGS18. This vector, designated pJH1, contains an aminogly-coside phosphotransferase gene (aph) from Tn903, a lacZ' gene for $\alpha$-complementation and a versatile multiple cloning site possessing unique restriction sites for EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, BspMI, PstI, SphI, and HindIII. When pJH1 was transformed into E. coli DHS${\alpha}$ and into P. putida HK-6, it was episomally and stably maintained in both strains. In addition, the enhanced green fluorescent protein (EGFP) gene which was transcriptionally cloned into pJH1 rendered E. coli cells fluorescence when its transformants were illuminated at 488 nm.

Crystallization and Electrical properties of $CuO-P_2O_5-V_2O_5$ Glass for solid state Electrolyte (고체 전해질용 $CuO-P_2O_5-V_2O_5$ 유리의 결정화와 전기 전도도)

  • Son, Myung-Mo;Lee, Heon-Soo;Chun, Yon-Soo;Gu, Hal-Bon;Lee, Sang-Geun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.07b
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    • pp.934-937
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    • 2003
  • Glasses in the system $CuO-P_2O_5-V_2O_5$ were prepared by a press-quenching method on the copper plate. The glass-ceramics from these glasses were obtained by post-heat treatment, and the crystallization behavior and DC conductivities were determined. The conductivities of the glasses were range from $10^{-6}s.Cm^{-1}$ at room temperature, but the conductivities of the glass-ceramics were $10^{-3}s.Cm^{-1}$ increased by $10^3$ order. The crystalline product in the glass-ceramics was $CuV_2O_6$. Heat-treatment conditions influenced the crystal growth of $CuV_2O_6$ and conductivity. The linear relationship between in (${\sigma}T$) and $T^{-1}$ suggested that the electrical conduction in the present glass-ceramics would be due to a small polaron hopping(SPH) mechanism.

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Procedural Fluid Animation using Mirror Image Method

  • Park, Jin-Ho
    • International Journal of Contents
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    • v.7 no.4
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    • pp.1-5
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    • 2011
  • Physics based fluid animation schemes need large computation cost due to tremendous degree of freedom. Many researchers tried to reduce the cost for solving the large linear system that is involved in grid-based schemes. GPU based algorithms and advanced numerical analysis methods are used to efficiently solve the system. Other groups studied local operation methods such as SPH (Smoothed Particle Hydrodynamics) and LBM (Lattice Boltzmann Method) for enhancing the efficiency. Our method investigates this efficiency problem thoroughly, and suggests novel paradigm in fluid animation field. Rather than physics based simulation, we propose a robust boundary handling technique for procedural fluid animation. Our method can be applied to arbitrary shaped objects and potential fields. Since only local operations are involved in our method, parallel computing can be easily implemented.