• Clinical and Experimental Reproductive Medicine
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• v.24 no.1
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• pp.67-81
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• 1997

To determine the concentration and the physiologic role of metal components in blood plasma and seminal plasma in relation to male infertility, the concentrations of twelve metal components in blood plasma and seminal plasma including Na, Mg, K, Ca, Cr, Mn, Fe, Cu, Zn, Se, Cd and Pb were measured by atomic absorbance spectrophotometery or ion selective electrode analysis. Semen and blood samples were obtained from a total of 110 men including 70 male infertility patients, 20 vasectomized persons and 20 fertility proven volunteers visited to the Male Infertility Clinic of Pusan National University Hospital. The concentrations of Ca, Zn, Mg, Cr and Cd in control group were higher in seminal plasma than in blood plasma, and additionally Pb were higher in infertility group. The concentrations of all metal components revealed no significant difference according to patients' age, resident, occupation, sperm density, motility and hormone level in blood plasma, but some metal components including Ca, Mg, Cu, Mn, Cd and Pb revealed a significant difference according to each these parameters except patient's age in seminal plasma. The concentrations of Mn, Cd and Pb in the vasectomy persons were higher than in the infertility group III including testicular and epididymal factors, but not in blood plasma. We conclude that the quantitative changes of metal components in the seminal plasma may have effects on not only spermatogenesis and sperm function, but also contribute to diagnostic parameter according to organ specificity of the metal in the male reproduction.

• Journal of Life Science
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• v.8 no.4
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• pp.400-409
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• 1998

Formation of adduct was studied in benzo(a)pyrene(BP)- and doxorubicin(Dx)-treated human NC-37 cells and isolated nuclei. Major adducts formed were determined by fluorescence absorption spectrophotometery and DNA-lin-ked protein assay. When isolated nuclei were exposed to carcinogens BP and DMBA, and anticancer drugs m-AMSA, ellipticine and Dx, varying degrees of adduct formation occured between DNA-protein complex and these drugs. When the mixture was centrifuged 1.7 M sucrose solution, binding BP and DMBA appeared to be similar between the sediment and the supernatant. When the sediment was centrifuged again with 0.35% polymin-P, the amount of BP bound was 2-fold greater in the protein(1077$\pm$55cpm) than in DNA fraction (470$\pm$20cpm), whereas that of DMBA was 1.6-fold greater in the DNA than in protein fraction. In the case of m-AMSA, ellipticine and Dx, the amount of binding was slightly greater in supernatant than in sediment in centrifugation with 1.7 M sucrose, and more than 3 times greater in the DNA- than in protein- fraction in centrifugation with 0.35% polymin P. DNA fractions which associated with a subset of nonhistone chromosomal protein were isolated from NC-37 cells exposed to $^{3}$H-BP and $^{14}$C-Dx. They were separated into two distince components DNA-S and DNA-P by centrifugation with 2M Nacl chromatin extraction. The results indicated that the amount of $^{3}$H-BP bound was 6.0-fold greater in DNA-P as compared with DNA-S, while that of $^{14}$C-Dx binding appreaed to be 6.2-fold greater in DNA-S than in DNA-P fraction. When $^{3}$H-BP binding wasdetermined in the presence of cold Dx, the amount of binding was reduced only in the DNA-P fraction, indicating that the interaction between DNA and protein is decreased. Gene expression by these drugs, BP treated cells were increased to compare with nomal cells but reduced by treatment with BP-Dx. These results suggest that the protein moiety which tightly bound to DNA-P fraction may play an important role in the regulation of gene expression.

• Analytical Science and Technology
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• v.6 no.1
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• pp.121-129
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• 1993

UV/VIS spectrophotometer interfaced with HPIC(High Performance Ion Chromatography) has been applied to the determination of lanthanide elements. The separation of lanthanide elements with HPIC helped to avoid erroneous analytical results due to interferences. Individual lanthanide elements at ppm level were separated on a HPIC CS5 column using pyromellitic acid and oxalic acid. The individual lanthanide elements were detected at 520nm following post-column reaction with PAR. Sm, Eu, Gd, Y, Tb, Dy, Ho, Er, Tm, Tb, and Lu were separated by pyromellitic acid. La, Ce, Pr and Nd were separated by oxalic acid. Appropriate pH of pyromellitic acid for separation was at pH 2.99.

• Journal of the Korean Chemical Society
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• v.53 no.2
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• pp.152-158
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• 2009

Spectrophotometric investigation of the interaction of Cefpodoxime proxetil (CFP) with $Ca^{2+},\;Mg^{2+},\;Mn^{2+},\;Fe^{3+},\;Co^{2+},\;Ni^{2+},\;Cu^{2+}$ and $Zn^{2+}$ in acidic medium showed the formation of 1:1 complex. The absorption spectrum of pure drug exhibits two prominent peaks at 270 and 345 nm. Its spectra scanned at several pH exhibited two isosbestic points (305 and 330 nm) indicating the presence of zwitterionic condition of drug in solution phase. The fluorescence emission spectra of CFP in presence of different concentrations of metal ions showed enhancement in fluorescence intensity which is ascribed to chelating enhancement fluorescence effect (CHEF). The stoichiometry of the complexes was determined by Job’s and Benesi-Hildebrand method. The stability of the complexes follow the order $Ca^{2+}\;<\;Mg^{2+}\;<\;Co^{2+}\;<\;Ni^{2+}\;<\;Zn^{2+}\;<\;Mn^{2+}\;<\;Cu^{2+}\;<\;Fe^{3+}$.

• Journal of the Korean Chemical Society
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• v.53 no.2
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• pp.144-151
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• 2009

An optode for cadmium ion determination has been designed by immobilization of dithizone on triacetylcellose membrane. When the optode membrane is introduced into a real samples containing cadmium, there is a color change from green to red, making it possible to use the change in absorbance at 611 nm as the analytical signal. The sensor could be used in the range of 0.3-3 ${\mu}g\;ml^{-1}$ (2.67-26.67 ${\mu}M$) of $Cd^{2+}$ ions with a limit of detection of 0.025 ${\mu}g\;ml^{-1}$ (25 ng $ml^{-1}$). The response time of optode is within 15 min depending on the concentration of $Cd^{2+}$ ions. It can be easily and completely regenerated by dilute EDTA solution. The effect of different possible interfering species has been examined and was shown the optode has a good selectivity. The results obtained for the determination of cadmium ion in polluted soil and water samples using the proposed optode was found to be comparable with the well-established atomic absorption method.

• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.730-736
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• 2007

The interaction of a polyoxometal (POM), K6SiW11Co(H2O)O39.10H2O (K6) as a Keggin, with human serum albumin (HSA) was studied by different methods and techniques. Binding studies show two sets of binding sites for interaction of POM to HSA. Binding analysis and isothermal calorimetery revealed that, the first set of binding site has lower number of bound ligand per mole of protein (ν), lower Hill constant (n), higher binding constant (K), more negative entropy (ΔS) and more electrostatic interaction in comparison to the second set of binding site. In addition, differential scanning calorimetery (DSC) and spectrophotometery data showed that, there are two energetic domains. The first domain is less stable (lower Tm and Cp) which corresponds to the tail segment of HSA and another with more stability is related to the head segment of HSA. Polyoxometal also decreases the stability of protein as Tm, secondary and tertiary structure as well as quenching of the fluorescence decrease. On other hand, perturbations in tertiary structure are more than secondary structure.

• Journal of the Korean Applied Science and Technology
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• v.13 no.1
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• pp.75-85
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• 1996

The absorption spectra of synthesis of long chain fatty acid containing azobenzene start from p-(p'-hydroxy phenyl azo)-benzoic acid was investigated by ultraviolet spectrophotometery in chloroform solvent at the various temperature. In addition, The pressure-area of the water-air interface was obtained and the LB film was fabricated onto a quartz slide and quartz crystal by conventional Langmuir-Blodgett(LB) method. The UV absorption spectra of Langmuir-Blodgett(LB) film on quartz slide and spectrum of monolayer formed on quartz crystal have been measured. Long chain fatty acid containing azobenzene are induced phtoisomerization by the application of u. v. and visible light irradiation alternatively the reversibility of phtoisomerization was more clear difference when the number of $C_{n}$ increased but, not so good at $C_{14}-azo$. At the pressure-area isotherms, the value of surface pressure increment were decreased when the number of $C_{n}$ increased. A surface pressure of 20mN/m was obtained as a proper one for a film deposition. The photoisomerization at LB films were induced by application of UV and visible light irradiation alternatively. So the LB film of long chain fatty acid containing azobenzene has possibility to being applied to functional molecular devices such as photomemory and light switching.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.3
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• pp.302-313
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• 2008

The purposes of this study are to investigate the concentration levels of fine particles, so called PM$_{2.5}$, to identify the affecting sources, and to estimate quantitatively the source contributions of PM$_{2.5}$. Ambient air sampling was seasonally carried out at two sites in Pohang(a residential and an industrial area) during the period of March to December 2003. PM$_{2.5}$ samples were collected by high volume air samplers with a PM$_{10}$ Inlet and an impactor for particle size segregation, and then determined by gravimetric method. The chemical species associated with PM$_{2.5}$ were analyzed by inductively coupled plasma spectrophotometery(ICP) and ion chromatography(IC). The results showed that the most significant season for PM$_{2.5}$ mass concentrations appeared to be spring, followed by winter, fall, and summer. The annual mean concentrations of PM$_{2.5}$ were 36.6 $\mu$g/m$^3$ in the industrial and 30.6 $\mu$g/m$^3$ in the residential area, respectively. The major components associated with PM$_{2.5}$ were the secondary aerosols such as nitrates and sulfates, which were respectively 4.2 and 8.6 $\mu$g/m$^3$ in the industrial area and 3.7 and 6.9 $\mu$g/m$^3$ in the residential area. The concentrations of chemical component in relation to natural emission sources such as Al, Ca, Mg, K were generally higher at both sampling sites than other sources. However, the concentrations of Fe, Mn, Cr in the industrial area were higher than those in the residential area. Based on the principal component analysis and stepwise multiple linear regression analysis for both areas, it was found that soil/road dust and secondary aerosols are the most significant factors affecting the variations of PM$_{2.5}$ in the ambient air of Pohang. The source apportionments of PM$_{2.5}$ were conducted by chemical mass balance(CMB) modeling. The contributions of PM$_{2.5}$ emission sources were estimated using the CMB8.0 receptor model, resulting that soil/road dust was the major contributor to PM$_{2.5}$, followed by secondary aerosols, vehicle emissions, marine aerosols, metallurgy industry. Finally, the application and its limitations of chemical mass balance modeling for PM$_{2.5}$ was discussed.