• Title/Summary/Keyword: Specific Growth Rate

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Growth Rate and Yield of a Methanotrophic Bacterium Methylosinus Trichosporium OB3b : I. Experimental Measurements (메탄자화균 Methylosinus trichosporium OB3b의 성장 속도와 수율 : I. 실험적 고찰)

  • 황재웅;송효학;박성훈
    • KSBB Journal
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    • v.13 no.4
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    • pp.391-398
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    • 1998
  • The effect of culture medium copper availability on the specific growth rate(${\mu}$) and carbon conversion efficiency (CCE) was sutided for an obligatory methanotroph Methylosinus trichosporium OB3b under various combinations of carbon and nitrogen sources. Methane or methanol was used as a carbon source, and nitrate or ammonium was used as a nitrogen source. Medium copper availability determined the intracellular location or kind of methane monooxygenase (MMO), cell-membrane (particulate or pMMO) when copper was present and cytoplasm (soluble or sMMO) when copper was deficient. When methane was used as a carbon source, copper-containing medium exhibited higher ${\mu}$ and CCE than copper-free medium regardless of the kind of nitrogen source. When methanol was used as a carbon source, however, the effect of copper disappeared. Ammonium gave the higher ${\mu}$ and CCE than nitrate for both methane and methanol. Those observation suggest that there exist an important difference in energy utilization efficiency for methane assimilation between sMMO and pMMO.

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The effect of dry matter allocation at the early seedling stare on the growth of two grass species (두 초본 종의 생장에 있어서 실생 초기의 물질분해 효과)

  • Park, Young-Mok
    • The Korean Journal of Ecology
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    • v.15 no.3
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    • pp.297-309
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    • 1992
  • The growth of digitaria adscendens and eleusine indica, annual weeds common in japan, was experimentally analyzed under varying untrient conditions. compared with e. indica, d. adscendensshowed a higher the two under high nitrogen availability. Higher net assimilation rate(nar) in d. adscendens than e. india was responsible for high RGR in D. adscendens under the low nitrogen availability. The different of NAR in the two species was induced not by leaf nitrogen content but by nitrogen use efficiency. Under unfertilized conditions nitrogen uptake rate(nur) was greater in d. adscendens than e. indica. Specific absorption rate in two species was similar, but root mass was greater in d. adscendens than e. indica.d. adscendens allocated more dry matter to roots than e. india earlier stage of seedlings. The contributed to higher rot mass and in turn resulted in higher nur in d. adscendens than that in e. india. It is concluuded that the larger allocation of dry matter to roots at early seedling stage in d. adscendens plays an important role in obtaining nitrogen for the continuation of growth under low nitrogen availability.

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A Study of the growth rate of licensed dental technicians by sex and the state of sex ratio on the year basis (성별에 따른 치과기공사 면허등록자의 성장비 및 연도별 성별비 현황조사 연구)

  • Kwon, Soon-Seog
    • Journal of Technologic Dentistry
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    • v.28 no.1
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    • pp.103-120
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    • 2006
  • In this study, we investigated the growth rate on the basis of the sex and the year among the subjects of the licensed dental technicians from the year 1970 to 2003 in Korea. In line with this investigation, this study aims to provide a basic material with regard to the state of dental technician workforce by sex and to suggest a need to reassignment of workforce by sex in accordance with the specific job description. The results of the study are as follows; First, among the licensed dental technicians from 1970 to 2003, males came to 11,589 and females, 5,976, which shows the number of males are 1.9 times higher than that of females. To put the mean sexual composite from 1970 to 2003, male was 82.9% and female 17.1%, indicating the number of males are 4.8 higher than that of females. Second, Of the mean increasing rate until the year 2003 in contrast to 1970, male was about 351 and female was about 181, which shows the number of males increased 1.94 times higher than the females. Comparing to the mean increasing rate of the last year, female came to 25.56% and male 10.73%, which reveals that the females were about 2.4 times higher than the males. In the case of the growth rate, females increased by 1,196,20 times and the males, 26,41. As a result the growth of females are about 45.3 times higher than the males. Third, in the case of the growth rate from 1980 to 2003, females came to 47.85 times and males 9.16, which shows the growth rate of the females was about 5.2 times higher than the males. In contrast to the year 1987, the growth rate until 2003 shows that the females came to 5,13 times and the males 2.70, which indicates the growth rate of females was about 1.9 times higher than males. Fourth, Compared to the year 1990, the growth rate until 2003 shows that females came to 3.40 times and males 2.03 indicating the growth rate of the females was about 1.7 times higher than males. In contrast to the year 2000, the growth rate till 2003 shows that females was 1,34 times and males 1.15 times increased. Fifth, the ratio of sex by the year among the licensed dental technicians is as follows; ratio of male to female in 1970 was 91.2 to 1. In 2003 the ratio came to 2.0 to 1. The number of growth by sex in 1970 came to 24.0 males to 1 female. But the figure was equalled in 2003.

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Optimum Cultivation Condition of Dunaliella Salina: Effects of Light Emitting Diode as a Lighting Source, Temperature, Light Intensity and Air Flow Rates (해양미세조류 Dunaliella Salina 최적 배양을 위한 연구: LED 조명, 온도, 광도 및 공기주입 속도에 따른 효과를 중심으로)

  • Choi, Boram;Kim, Dongsu;Lee, Taeyoon
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.9
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    • pp.630-636
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    • 2012
  • The purpose of this study was to determine optimum wavelength, light intensity, aeration rate, and temperature for the cultivation of Dunaliella salina illuminated by various types of light emitting diode. Growth rates of Dunaliella salina were faster at higher temperature than the growth rate at lower temperature. Among the culturing temperatures, $22^{\circ}C$ was the optimum temperature for the growth of Dunaliella salina. White LED was the most efficient light source and lower light intensity (3,000 Lux) resulted in better biomass production (1.30 g/L). The value of aeration varied between 0 and 2.4 vvm at the illumination of 3,000 Lux of white light emitting diode. Highest specific growth rate of $1.12day^{-1}$ was obtained at no-aeration and lower specific growth rates were obtained for other aeration tests, which indicated that aeration could be harmful for the cultivation of Dunaliella salina.

Effects of Limiting Factors on Cultivation of Chlorella Sp. under Red Light Emitting Diode: Light Intensity, Blowing of Air or Carbon Dioxide (적색 발광다이오드(Light Emitting Diode)를 이용한 클로렐라 배양에 미치는 영향인자 분석: 빛세기, 공기 및 이산화탄소 주입)

  • Choi, Boram;Lee, Taeyoon
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.2
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    • pp.41-47
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    • 2012
  • The purpose of this study was to determine optimum condition for the cultivation of Chlorella sp. FC-21 using red light emitting diodes (LED). Specific growth rate and cell concentration were measured for the reactors at the illuminations of different light intensity of red LED. Under the illumination of red LED, specific growth rate increased as light intensity increased but cell concentrations decreased. To determine beneficial effect of aeration to cell cultivation, micro-air bubbles were aerated at 0.7 vvm in the reactor at the illumination of red LED. Two and ten times greater specific growth rate and cell concentration were obtained when aeration was applied. In case of blowing of carbon dioxide, pH of culture medium decreased below to pH 3, which resulted in decreases of cell concentration. From this study, we found that red LED with aeration were the most appropriate light source for the cultivation of Chlorella sp. FC-21.

Production and Characteristics of Cellulase from Sporocytophaga congregata and Mixed Culture with Yeast (Sporocytophaga congregata에 의(依)한 Cellulase 생산(生産) 및 그 효소특성(酵素特性)과 효모(酵母)와의 혼합배양(混合培養))

  • Kim, Chang-Jin;Kim, Sang-Soon;Lee, Ke-Ho
    • Applied Biological Chemistry
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    • v.29 no.1
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    • pp.36-43
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    • 1986
  • In order to produce cellulosic single cell protein from the cellulose, 163 strains of cellulose assimilating bacteria were isolated from 95 sources and one of them was screened by its strong cellulose assimilating activity. and was identified as Sporocytophaga congregate A-7. The optimum temperature and pH for cellulase production were $30^{\circ}C$ and 6.0, and the optimum temperature, pH and heat stability of the enzyme were $50^{\circ}C$, 7.0 and below $55^{\circ}C$. When the bacteria was cultured in fermentor, the specific growth rate was $0.034hr^{-1}$ and when the bacteria was mixed cultured with Candida guilliermondii var. guilliermondii, the specific growth rate of the bacteria and yeast were $0.06hr^{-1}$ and $0.08hr^{-1}$ respectively and total cell dry weight was $4{\sim}5g/l$.

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The Toxic Effects of Parathion on Survival and Growth of Juvenile Bagrid Catfish (Pseudobagrus fulvidraco) (동자개 (Pseudobugrus fulvidraco) 치어의 생존 및 성장에 미치는 Parathion의 영향)

  • CHO Kyu-Seok;PARK Jong-Ho;JEE Jung-Hun;KANG Ju-Chan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.5
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    • pp.380-384
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    • 2004
  • The toxic impact of parathion on bagrid catfish (Pseudobagrus fulvidraco) juvenile was examined 96 hr $LC_{50}$ value of parathion estimated as 1 90 mg/L. Chronically, bagrid catfish were exposed for 30 days to concentrations of parathion, i.e. 63, 95, 190 and $380\;{\mu}g/L$, representing 3.3, 5.0, 10.0 and $20.0{\%}$ of the 96 hr $LC_{50}$. Survival rate was significantly affected by the concentration over $63\;{\mu}g/L$ after 10 days. Growth rate, specific growth rate (SGR) and feed efficiency were reduced in time as concentration dependent manner. Growth rates of the exposed groups were significantly reduced as $28.06-60.63{\%}$ to the control fish, SGR was decresed as $1.50-4.72{\%}$ and feed efficiency was declined as $6.10-11.90{\%}$. It can be concluded that if p. lulvidraco is chronically exposed to parathion over $63\;{\mu}g/L$ concentration, the physiological damages might affect growth factors of the fish.

Comparative Evaluation of Modified Bioreactors for Enhancement of Growth and Secondary Metabolite Biosynthesis Using Panax ginseng Hairy Roots

  • Jeong, Gwi-Taek;Park, Don-Hee
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.528-534
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    • 2005
  • Hairy root cultures have demonstrated great promise in terms of their biosynthetic capability toward the production of secondary metabolites, but continue to constitute a major challenge with regard to large-scale cultures. In order to assess the possibility of conducting mass production of biomass, and the extraction of useful metabolites from Panax ginseng. P. ginseng hairy roots, transformed by Rhizobium rhizogenes KCTC 2744, were used in bioreactors of different types and sizes. The most effective mass production of hairy roots was achieved in several differently Sized air bubble bioreactors compared to all other bioreactor types. Hairy root growth was enhanced by aeration, and the production increased with increasing aeration rate in a 1 L bioreactor culture. It was determined that the hairy root growth rate could be substantially enhanced by increases in the aeration rate upto 0.5vvm, but at aeration rates above 0.5vvm, only slight promotions in growth rates were observed. In 20 L air bubble bioreactors, with a variety of inoculum sizes, the hairy roots exhibited the most robust growth rates with an inoculum size of 0.1% (w/v), within the range 0.1 to 0.7% (w/v). The specific growth rates of the hairy root decreased with increases in the inoculum size.

Fish farm monitoring report for outdoor aquaculture of far eastern catfish Silurus asotus in Korea

  • Hyeongsu Kim;Jongsung Park;Bokki Choi
    • Fisheries and Aquatic Sciences
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    • v.26 no.11
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    • pp.660-668
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    • 2023
  • This study aimed to investigate the growth performance of far eastern catfish (Silurus asotus) on outdoor fish farms to obtain basic data for the domestic eastern catfish aquaculture industry. An outdoor fish farm was directly monitored from June 2018 to October 2019 to determine the farming conditions, growth performance, and water quality. The growth performance in 2017 was analyzed using data from the same fish farm. Three years of monitoring showed that the fish farm required approximately 5-6 months between stocking, harvesting, and selling an S. asotus batch. The growth parameters, namely, the weight gain rate (WGR), specific growth rate (SGR) for culture periods, SGR for feeding periods, and feed coefficient rate (FCR), were 4,664.7%, 1.27%, 2.43%, and 1.25 in 2017; 6,452.0%, 1.52%, 2.79%, and 1.42 in 2018; and 3,270.0%, 1.11%, 2.12%, and 1.38 in 2019, respectively. Moreover, the WGR was two-fold higher in 2018 than 2019, whereas the FCR was more effective in 2019 than 2018, presumably because of the stocking density. No mass mortality was observed during the water quality analysis. The results of this study provide basic data for the development of the catfish industry.

Application of Oxygen Uptake Rate Measured by a Dynamic Method for Analysis of Related Fermentation Parameters in Cyclosporin A Fermentation:Suspended and Immobilized Cell Cultures

  • Chun, Gie-Taek;Agathos, S.N.
    • Journal of Microbiology and Biotechnology
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    • v.11 no.6
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    • pp.1055-1060
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    • 2001
  • Experimental data for the on-line estimation of cell concentration and growth rate are presented. For this purpose, we utilized the on-line calculation of the oxygen uptake rate (OUR), which was derived from a liquid phase dynamic mass balance for the oxygen during the active growth phase in cyclosporin A (CyA) fermentation. The cell yield coefficient, based on the oxygen $(Y_{x/o})$for both suspended and immobilized cells of Tolypocladium inflatum, was estimated as $1.9 gDCW/gO_2$ from a very good linear correlation between the cell mass produced and the total oxygen consumed. The calculated yield showed a good agreement with the value of $(Y_{x/o})$ generated from the correlation between the cell growth rate and the oxygen uptake rate. In addition, further experimental data are given, which were also applied to determine the specific oxygen uptake rate of T. inflatum cells during the exponential phase of CyA fermentation. A theoretical basis for the analysis of these fermentation parameters is also provided.

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