• Journal of fish pathology
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• v.26 no.3
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• pp.303-310
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• 2013

Since March in 2013, Inland Aquaculture Research Center, NFRDI has cultivated 1,000 wild swamp eel(Monopterus albus) for species conservation research. While cultivating, 100 fishes showed clinical sign that darkness color, mucus hypersecretion and anus rubor. Even some of them were died. Result of anatomical test, all 100 fishes were infected with intestinal parasite, acanthocephalan. So we were going to determine the case of acanthocephalans infection in swamp eel(Monopterus albus) as histopathologically. Acanthocephalan was founded in alimentary canal only. Parasite were confirmed 19 unit in individual fish, averagely. Heavy infected fishes were confirmed enterocleisis by acanthocephalans. Worms were attachment in submucosa layer of alimentary canal by invading proboscis. Characteristic symptoms were observed in stomach and intestine, including hyperemia in mucous epithelium, infiltration of eosinophills in submucosa layer, inflammation, parasitic granuloma. Some fishes showed vacuolization of gastricgland epithelium, necrosis of intestinal mucosa. Other organs, excluding alimentary canal, were not found lesion. The results of this study, the effect of the acanthocephalan infection on swamp eel(Monopterus albus) could find and it seems to be a big help in the future swamp eel(Monopterus albus) cultivation.

• Korean Journal of Plant Taxonomy
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• v.47 no.3
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• pp.264-288
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• 2017

In order to organize a new checklist for endemic vascular plants of the Korean Peninsula, 358 taxa of the Korea Forest Service and 375 taxa of the National Institute of Biological Resources were reviewed. In addition, the names of previously published endemic plants above the rank of variety were checked up to 2016. From the two lists, a total of 444 taxa were identified. Among them, 289 taxa were common on the two lists, but 69 and 86 taxa were listed only by the Korea Forest Service and by the National Institute of Biological Resources, respectively. Taxonomic documents including journal papers and dissertations were examined with regard to the 444 taxa. In some cases, opinions of Korean experts in this field were also reflected. From the results, 312 taxa in total were recognized as plants endemic to the Korean Peninsula, while 132 taxa could not be included on the endemic list. On the other hand, 48 taxa, none of which existed on the two lists, were newly listed. In conclusion, the new checklist of vascular plants endemic to Korea was confirmed, with a total of 360 taxa consisting of 64 families, 172 genera, 297 species, 4 subspecies, 51 varieties and 8 hybrids. The new common name Halla-seol-aeng-cho is given to Primula farinosa L. subsp. modesta (Bisset & S. Moore) Pax var. hannasanensis (T. Yamaz.) T. Yamaz. Sillaphyton Pimenov (Deok-u-gi-reum-na-mul-sok) is newly identified as a genus endemic to Korea. However, Pentactina Nakai could not be included on the list of Korean vascular endemic genera.

• Korean Journal of Plant Resources
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• v.20 no.5
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• pp.451-460
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• 2007

This study was carried out to construct the data base for biodiversity conservation, and offer the raw data for obtaining the genetic resources. The vascular plants were summarized as 306 taxa; 79 families, 199 genera, 266 species, 35 varieties and 5 forma. The Korean endemic plants were 7 taxa; Salix caprea, S. purpurea var. japonica, Deuitzia coreana, Akebia quinata var. plyphylla, Spiraea prunifolia for. simpiciflora, Lonicera subsessilis and Weigela subsessilis. The naturalized plants were 16 taxa; Dactylis glomerata, Rumex acetocella, R. crispus, Phytolacca esculenta, Tlaspi arvense, Robinia pseudo-acacia, Trifolium repens, Ailanthus altissima, Oenothera lamarckiana, Veronica persica, Ambrosia artemisiifolia var. elatior, Erigeron annuus, Erigeron canadensis, Bidens frondosa, Taraxacum officinale and Sonchus asper. The specific plants by floristic region were 26 taxa; Chloranthus japonica, Populus davidiana, and so forth.

• Korean Journal of Heritage: History & Science
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• v.54 no.4
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• pp.78-89
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• 2021

Organic residues analysis is an analysis method that reveals the types of organic material samples by using the characteristic that main components constituting substances are different depending on the species of animals and plants. In this study, scientific analysis of the organic residues attached to wood coffins in the Joseon Dynasty Hoemyo excavated from the site of Singok-dong, Uijeongbu was used to identify the types of remnants and to use them as information to restore the uses of organic materials and the way they lived in the past. As a result of FT-IR analysis of the residue attached to the inside of the wood, it was estimated to be a natural plant resin material. In addition, as a result of analysis by GC-MS to confirm the characteristic factors of natural resins, diterpenoids (abietane) and pimaran (pimarane), such as dehydroabietic acid and pimaric acid (diterpenoid) compounds, and saturated and unsaturated fatty acid components were detected together. Diterpenoid compounds are components mainly found in Pinaceae resins. It is confirmed in the literature that rosin, a representative material of Pinaceae resin, was used as an adhesive material. Considering the situation where an organic material remained at the joint of the wood, the organic material attached to the wood is judged to be an adhesive material made of Pinaceae resin. In addition, the fatty acid component detected together is a component derived from plant oil, and it is presumed to be made by mixing rosin and oil as recorded in previous studies. This study confirms that organic residues remain in the burial environment without losing their characteristics. It is expected that scientific analysis of organic residues will be conducted in the future to accumulate information necessary for the interpretation of past living culture.

• Genes and Genomics
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• v.40 no.12
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• pp.1319-1329
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• 2018

SSRs were successfully isolated from the Perilla crop in our current study, and used to analyze Perilla accessions from East Asia. Analyses of the clear genetic diversity and relationship for Perilla crop still remain insufficient. In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 alleles per locus and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from 0.229 to 0.943, with an average of 0.466. The average polymorphic information content (PIC) value was 0.603, ranging from 0.102 to 0.837. The genetic diversity (GD) ranged from 0.108 to 0.854, with an average of 0.654. Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. This study demonstrated the utility of new SSR analysis for the study of genetic diversity and population structure among 35 Perilla accessions. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. The new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of the cultivated and weedy types of P. frutescens in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/QTLs for Perilla breeding programs.

• Korean Journal of Environment and Ecology
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• v.32 no.6
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• pp.591-602
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• 2018

Clithon retropictus has been designated as an endangered wildlife Class II due to its high value as a biological indicator species capable of judging environmental quality such as salinity, water flow, and ground conditions. However, basic research on its physiological and ecological characteristics is still lacking. As such, this study intended to examine the impact of environmental conditions such as salinity and soil particle size on the size and density of Clithon retropictus at the Yeoncho river estuary. The investigation of the salinity, which is a key variable that affects the distribution of organisms in the estuary, showed that Clithon retropictus could grow at a salinity ranging from 0#x2030; (freshwater) to 25‰ (brackish water). The coarse gravel (19-75mm) tended to increase nearer the upper stream (under the Yeoncho weir), while the proportion of particles smaller than sand (less than 19mm) increased toward the downstream. The population and the size of the individuals decreased rapidly in the downstream where water stagnated near the Yeoncho weir, and the salt water joined. The results indicated that Clithon retropictus had a high tolerance to salinity, but the adaptability was weaker toward the extremes since the population, and the size tended to decrease as the salinity increased. The correlation analysis revealed that both salinity and soil particle size affected the population and individual size. The correlation between the individual size and salinity was -0.242 (P <0.01), indicating that the size decreased with increasing salinity. The correlation between individual size and coarse gravel having a particle size of 19mm or more was 0.420 (P <0.01), indicating that the size increased with increasing the particle size.

• Journal of Life Science
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• v.29 no.7
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• pp.762-768
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• 2019

Doellingeria scabra Thunb. (syn. Aster scaber Thunb.), a perennial herb in the family Asteraceae, is frequently found in the wild mountain regions of Korea. This aim of this work was to measure the characteristics of patchiness of D. scabra in a local population on Mt. Maebong in Taeback-ci, Gangwon-do. The spatial distribution pattern of this species was estimated by analyzing ecological data by methods including the index of dispersion, Lloyd's mean crowding, and Morisita's index. The mean population density of the D. scabra population was 2.94. The D. scabra individuals were uniformly or randomly distributed in small-scale plots and were aggregately distributed in two large-scale plots ($16{\times}32m^2$ and $32{\times}32m^2$). The mean crowding ($M^*$) was 0.916. The mean patchiness index (PAI) was 0.796. Morisita's coefficient tended to decrease the density of the population as the plot size increased. The expected value of Eberhardt's index ($I_E$) in the local population was 2.623. Moran's I of D. scabra significantly differed from the expected value in 6 of 8 cases (75.0%). The first five classes were positive, with four showing statistical significance, indicating similarity among individuals in the first four distance classes (I-IV, 8 m), The results presented here could provide a theoretical basis for the conservation of D. scabra (Korean: chamchwi) and for the rehabilitation and sustainable management of forest ecosystems on Mt. Maebong, as well as on other mountains.

• Korean Journal of Heritage: History & Science
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• v.45 no.1
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• pp.174-189
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• 2012

This study aims to examine the traditional living space to reflect the environment-friendly design methods and principles of reestablishment. To do so, this study carried on a related literature study and field survey. The traditional living space in terms of the environmental friendliness is reflected on site selection and space composition, and utilization of natural energy and natural materials. Focused on the Kwangajeong in Yangdong Village, this study is to identify eco-friendly techniques on the traditional living space. As a result, it shows that Kwangajeong with the side of the southeast in site selection had been considered in aspect of environmental control such as sunshine and solar radiation. Also building construction techniques had been used to minimize the variation of terrain. In aspect of environmental conservation, Kwangajeong had structurally arranged the gate, an inner yard and an inner floor to allow good ventilation. In aspect of space composition, Kwangajeong with the emphasis of scenic view had utilized the methods that attract external landscape through the control of its fences. Environment-friendly techniques and control principles in traditional space had been developed by a long experience through the long periods and the traditional techniques suitable for local climate and local environment have been developed. The technical development of these techniques to resolve the global environmental and energy issues and create a more pleasant living environment of human beings might be critical.

• Korean Journal of Heritage: History & Science
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• v.43 no.1
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• pp.86-99
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• 2010

Among Natural monument birds, a total of 11 species and 2,209 individuals were found in Nakdong river estuary from March 2007 to February 2008 including Eurasian Oystereacher (Haematopus ostralegus; No. 326), Mandarin Duck (Aix galericulata; No.327), Tundra Swan (Cygnus columbianus; No.201-1), Whooper Swan (Cygnus cygnus; No.201-2), Black-faced Spoonbilll (Platalea minor; No.205-1), Eurasian Spoonbill (Platalea leucorodia ; No.205-2), Steller's Sea Eagle (Haliaeetus pelagicus; No.243-3), White-tailed Sea Eagle (Haliaeetus albicilla ; No.243-4), Hen Harrier (Circus cyaneus; No.323-6), Common Kestrel (Falco tinnunculus; No.323-8) and Swan Goose (Anser cygnoides; No.325-1). Among the habitats for migratory birds in Korea, the Nakdong river estuary is the only place where more than 2,000 individuals of Whooper Swan(Cygnus cygnus) spend winter. Eulsukdo, Myeongji Flat and neighboring tidal flats (Myeonggeummeori, Baikhapdeung, Doyodeung, Jangjado, etc.) are major habitats for natural monument birds such as Eurasian Spoonbill(Platalea leucorodia ), Whooper Swan(Cygnus cygnus) and Swan Goose(Anser cygnoides). A colony of aquatic plants such as Common Bulrush(Scirpus triqueter) and Common Reed(Phragmites communis) serves as a feeding and resting place for birds in the Anatidae family including ducks, swans and geese. Plans for management and conservation of the habitats and the natural monument birds in Nakdong river estuary are as follows: minimization of water contamination, construction and management of aquatic plants habitats, protection of sandbanks, security of stable habitats, and consistent monitoring.

• Molecules and Cells
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• v.42 no.9
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• pp.672-685
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• 2019

Currently, liver transplantation is the only available remedy for patients with end-stage liver disease. Conservation of transplanted liver graft is the most important issue as it directly related to patient survival. Carbonyl reductase 1 (CBR1) protects cells against oxidative stress and cell death by inactivating cellular membrane-derived lipid aldehydes. Ischemia-reperfusion (I/R) injury during living-donor liver transplantation is known to form reactive oxygen species. Thus, the objective of this study was to investigate whether CBR1 transcription might be increased during liver I/R injury and whether such increase might protect liver against I/R injury. Our results revealed that transcription factor Nrf2 could induce CBR1 transcription in liver of mice during I/R. Pre-treatment with sulforaphane, an activator of Nrf2, increased CBR1 expression, decreased liver enzymes such as aspartate aminotransferase and alanine transaminase, and reduced I/R-related pathological changes. Using oxygen-glucose deprivation and recovery model of human normal liver cell line, it was found that oxidative stress markers and lipid peroxidation products were significantly lowered in cells overexpressing CBR1. Conversely, CBR1 knockdown cells expressed elevated levels of oxidative stress proteins compared to the parental cell line. We also observed that Nrf2 and CBR1 were overexpressed during liver transplantation in clinical samples. These results suggest that CBR1 expression during liver I/R injury is regulated by transcription factor Nrf2. In addition, CBR1 can reduce free radicals and prevent lipid peroxidation. Taken together, CBR1 induction might be a therapeutic strategy for relieving liver I/R injury during liver transplantation.