• Title/Summary/Keyword: Spatio-temporal Distribution

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Selective and Random Patterning of Programmed Cell Death in Zebrafish Embryonic Development

  • Hwang, Chang-Nam;Kim, Joon;Lee, Sang-Ho
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.118-118
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    • 2002
  • Programmed cell death (PCD) is thought as a well-controlled process by which unwanted cells are selectively eliminated. During the last decade many researches have elucidated molecules and their interactions involved in cell death by using largely in vitro induction of cell death or survival signals in a more defined manner, While these critical information and novel findings provide us with clearer understanding of mechanisms underlying cell death, it does by no means explain how PCD occurs and which cells or tissues are affected during normal embryonic development in vivo. In this study, we used zebrafish to examine whether the PCD is occurring selectively or randomly in developing embryos by whole mount in situ TUNEL analysis with specific markers for neural cells. The result revealed that the degree and distribution of TUNEL staining varied considerably throughout gastrulation stage, and there was also a number of TUNEL-negative embryos. Most of TUNEL-positive cells were scattered randomly throughout the blastoderm. During the gastrulation stage about 75 % of the embryos analyzed exhibited more than 5 TUNEL-positive cells. As the dorsal epiblast begins to thicken rather abruptly near the end of gastrulation, TUNEL-positive cells were mainly located along the dorsal side. Although there were some variations in TUNEL staining during segmentation and pharyngeal stages, TUNEL staining continued to be localized to the central nervous system, and was also detected in the sensory organs, trigeminal ganglions, and the primary sensory neurons. High levels of the cell death in developing brain between 20-somite and prim-6 stages are thought to play a role in the morphogenesis and organization of the brain. At prim-16 stage, cell death is considerably reduced in the brain region. Dying cells are mainly localized to the prospective brain region where ectodermal cells are about to initiate neurogenesis. As development progressed, high levels and more reproducible patterns of cell death were observed in the developing nervous system. Intensive TUNEL staining was restricted to the trigeminal ganglions, the primary sensory neurons, and sensory organs, such as olfactory pits and otic vesicles. Thus, PCD patterning in zebrafish embryos occurs randomly at early stages and becomes restricted to certain region of the embryos. The spatio-temporal pattern of PCD during the early embryonic development in zebrafish will provide basic information for further studies to elucidate genes involved in. regulation of PCD largely unknown in vivo during vertebrate embryogenesis.

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Projection of Future Snowfall and Assessment of Heavy Snowfall Vulnerable Area Using RCP Climate Change Scenarios (RCP 기후변화 시나리오에 따른 미래 강설량 예측 및 폭설 취약지역 평가)

  • Ahn, So Ra;Lee, Jun Woo;Kim, Seong Joon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.35 no.3
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    • pp.545-556
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    • 2015
  • This study is to project the future snowfall and to assess heavy snowfall vulnerable area in South Korea using ground measured snowfall data and RCP climate change scenarios. To identify the present spatio-temporal heavy snowfall distribution pattern of South Korea, the 40 years (1971~2010) snowfall data from 92 weather stations were used. The heavy snowfall days above 20 cm and areas has increased especially since 2000. The future snowfall was projected by HadGEM3-RA RCP 4.5 and 8.5 scenarios using the bias-corrected temperature and snow-water equivalent precipitation of each weather station. The maximum snowfall in baseline period (1984~2013) was 122 cm and the future maximum snow depth was projected 186.1 cm, 172.5 mm and 172.5 cm in 2020s (2011~2040), 2050s (2041~2070) and 2080s (2071~2099) for RCP 4.5 scenario, and 254.4 cm, 161.6 cm and 194.8 cm for RCP 8.5 scenario respectively. To analyze the future heavy snowfall vulnerable area, the present snow load design criteria for greenhouse (cm), cattleshed ($kg/m^2$), and building structure ($kN/m^2$) of each administrative district was applied. The 3 facilities located in present heavy snowfall areas were about two times vulnerable in the future and the areas were also extended.

Zooplankton Community and Distributions of Copepods in Relation to Eutrophic Evaluation in Chinhae Bay (진해만 수질 환경과 동물플랑크톤 군집 및 요각류 분포 특성)

  • KANG Young-Shil;PARK Joo-Suck;LEE Sam-Seuk;KIM Hak-Gyoon;LEE Phil-Yong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.4
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    • pp.415-430
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    • 1996
  • Spatio-temporal variations in zooplankton community and ropepod indicator species were investigated along with the interaction between zooplankton distribution and environmental factors in Chinhae Bay. Zooplankton samples were monthly collected at 7 stations from February to September in 1993. A NORPAC net was vertically hauled from bottom to surface, At the same station, environmental factors such as temperature, salinity and COD (chemical oxygen demand) were measured at two different water layers, surface and bottom. In August and September, salinity declined below 30.00‰ , while eutrophic parameters such as COD showed the higher concentrations than those in other months, with higher concentrations at inner bay stations. Salinities were, however, higher at bay mouth areas. These distributional patterns were believed to be caused by input and dispersion of organic matters from nearby land. Zooplankton communities were composed of 7~14. Of these, Noctiluca scintillans was predominant and occupied 90.6‰ of total zooplankton abundance. Cladocera and Copepoda were secondly abundant taxa. Among 6 to 10 copepod species appeared, Acartia omorii and A. hudsonics were most common species during the survey months except March and September. Species diversities were greater, in general, at inner bay than outer bay. The lowest diversity index was observed in February, while the highest in July. Cluster analysis could divide the study area into 2 or 4 zones for each month. Zone 1, mouth area of the bay, was characterized by the influence of offshore waters. Zone II was mixing area. Zone III and IV seemed to be affected by nearby land.

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How are the Spatio-Temporal Distribution Patterns of Benthic Macrofaunal Communities Affected by the Construction of Shihwa Dike in the West Coast of Korea? (시화방조제의 건설은 저서동물군집의 시${\cdot}$공간 분포에 어떠한 영향을 미쳤는가?)

  • HONG Jae-Sang;JUNG Rae-Hong;SEO In-Soo;YOON Kon-Tak;CHOI Byong-Mee;YOO Jae-Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.5
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    • pp.882-895
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    • 1997
  • Changes in the benthic communities have been studied to investigate the environmental effects before and after the construction of Shihwa Dike in the West coast of Korea. It is suggested that sequential changes in macrofaunal assemblages progressed in two sucressional directions. In the Shihwa lake under the influence of organic enrichment. First, the appearance of 'azoic tone' or 'grossly polluted zone' developed in the area of less than 6 m in depth resulted from the severe dissolved oxygen depletion due to the eutrophication from the increased organic loading. Second, the 'polluted zone' characterized by the proliferation of the opportunistic species in organically enriched area, was found in the vicinity of the industrial discharges and nearby fluvial inputs. This benthic community succession in the Shihwa lake seemed to be caused by the various ecological events such as an eutrophication in this organically enriched environment after construction of the dike and other physico-chemical parameters like salinity and dissolved oxygen in the bottom water, which may be influenced by the irregular surface water discharge and dilution by outer seawater inflow through the water gate of the dike. On the other hand, the benthic communities in the outside of the dike showed that the species richness was more than doubled and the abundance increased almost seven times more than that before the dike construction. This may be a typical characteristics of the initial phase in benthic eutrophication, suggesting that an increased organic input area may have been reponsible for this faunal change in the study area.

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Characteristics of Long-term Variability of the Net Heat Flux on the Sea Surface in the East Asian Marginal Seas (동아시아 해역 해수면 순열속의 장기 변동 특성)

  • Lee, Seong-Wook;Na, Jung-Yul
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.5 no.2
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    • pp.86-94
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    • 2000
  • In order to extract the spatio-temporal characteristics of long-term variability of the net heat flux on the sea surface in the East Asian marginal seas, empirical orthogonal function (EOF) analysis was conducted using data set calculated every 12 hours interval during 1978-1995. Among the first three modes explaining 73% of the total variance, the first mode having high peak at 1 year period indicates high variability area around the Sandong Peninsula and central and northeastern part of the East Sea. In the second mode which has spatial distribution of dipole type at the north and south, the peaks appear at 3.6 year and 2.3 year cycles. Time coefficient of the second EOF is believed to have close relation with the E1 Nino and has out-of-phase variation with NINO3 SST. Lagged correlation between NINO3 SST and time coefficient of the second EOF indicates four month time delay in the NINO3 SST. In the third mode which has opposite sign at the east and west, the periodicity of 6-9 year cycle has relatively clear appearance compared to other two EOFs. Also, high heat loss exceeding 800 W/$m^{2}$ in winter time occured at the south part of the Sandong Peninsula and Vladivostok. It reveals more frequent occurrence of about two times at the Sandong Peninsula than Vladivostok. The event is concentrated in January at Vladivostok, but it occurs primarily in December and January at the Sandong Peninsula.

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Reordering Scheme of Location Identifiers for Indexing RFID Tags (RFID 태그의 색인을 위한 위치 식별자 재순서 기법)

  • Ahn, Sung-Woo;Hong, Bong-Hee
    • Journal of KIISE:Databases
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    • v.36 no.3
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    • pp.198-214
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    • 2009
  • Trajectories of RFID tags can be modeled as a line, denoted by tag interval, captured by an RFID reader and indexed in a three-dimensional domain, with the axes being the tag identifier (TID), the location identifier (LID), and the time (TIME). Distribution of tag intervals in the domain space is an important factor for efficient processing of a query for tracing tags and is changed according to arranging coordinates of each domain. Particularly, the arrangement of LIDs in the domain has an effect on the performance of queries retrieving the traces of tags as times goes by because it provides the location information of tags. Therefore, it is necessary to determine the optimal ordering of LIDs in order to perform queries efficiently for retrieving tag intervals from the index. To do this, we propose LID proximity for reordering previously assigned LIDs to new LIDs and define the LID proximity function for storing tag intervals accessed together closely in index nodes when a query is processed. To determine the sequence of LIDs in the domain, we also propose a reordering scheme of LIDs based on LID proximity. Our experiments show that the proposed reordering scheme considerably improves the performance of Queries for tracing tag locations comparing with the previous method of assigning LIDs.

Variability and Horizontal Structure of Sea Surface Height Anomaly Estimated from Topex/poseidon Altimeter in the East (Japan) Sea (동해의 Topex/Poseidon 고도계로부터 추정된 해면고도이상치의 수평구조와 변동성)

  • Kim, Eung;Ro, Young-Jae;Kim, Chang-Shik
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.94-110
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    • 2003
  • This study utilizes the dataset of Topex/Poseidon(T/P) altimeter sea surface height (1992-2000 yr., 286 cycles)to investigate the tempore-spatial variability in the East (Japan) Sea. Optimal interpolation (Ol) technique was applied to the pre-processed T/P dataset (level 2) to produce sea surface height anomaly (SSHA) map on regular grids. Spectral analyses of the timeseries of the SSHA at chosen stations and empirical orthogonal function (EOF) analysis of the SSHA in the entire East Sea were made. Distribution of the SSHA can be divided by the southern and northern regions sharply by the polar front situated in the middle of the East Sea. The southern region under the direct influence of the Tsushima Current exhibits higher amplitude of the SSHA fluctuation, while the northern region does relatively smaller one. The spatio-temporal variability of the SSHA in the East Sea can be characterized by the five modes of the EOFs accounting for more than 85% of the total variance. The first mode dominates the SSHA variation in the entire domain with strong seasonal and inter-annual periods accounting for the 72.3% of the total variance. The other modes (up to 5th account for 14%) are responsible for the SSHA variation associated with the local current system, meandering of the polar frontal axis, and mesoscale eddies. Spectral peaks with significant confluence level show semi-annual, annual and interannual (2, 3-4 years) periods.

Community Structures of Macrozoobenthos in the Subtidal Area of the Nakdong River estuary, Korea (낙동강 하구 조하대 대형저서동물의 군집구조)

  • SEO, JIN-YOUNG;KIM, JEONG-HYUN;CHOI, JIN-WOO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.21 no.3
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    • pp.112-124
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    • 2016
  • In order to see the spatio-temporal distribution patterns of macrobenthic fauna in subtidal areas of Nakdong River estuary, benthic fauna samples were collected seasonally at 8 sites in subtidal areas of Nakdong River estuary from February, 2013 to November, 2015. Sandy sediment facies was found in the southern part of the barrier islands in Nakdong River estuary while muddy sediment facies occupied at sites in offshore areas. The bottom salinity of the study area was measured greater than 30 psu during the study period. The mean species richness and density were estimated as 266 species and $859ind\;m^{-2}$, respectively. Polychaete worms were the most abundant fauna group, and four polychaete species such as Goniada maculata, Sternaspis chinensis, Magelona japonica, and Heteromastus filiformis occurred as dominant species in this study area. Pseudopolydora kempi known as an opportunistic species occurred dominantly only in August, 2013. From the multivariate analyses, the macrobenthic fauna in the subtidal area of the Nakdong River estuary could be divided into 2 assemblages that each occupied sites near barrier islands and offshore areas by sediment facies.

Derivation of Synergistic Aerosol Model by Using the ECMWF/MACC and OPAC (ECMWF/MACC와 OPAC자료를 이용한 시너지 에어로솔 모델 산출)

  • Lee, Kwon-Ho;Lee, Kyu-Tae;Mun, Gwan-Ho;Kim, Jung-ho;Jung, Kyoung-Jin
    • Korean Journal of Remote Sensing
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    • v.34 no.6_1
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    • pp.857-868
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    • 2018
  • The microphysics and spatio-temporal distribution of atmospheric aerosols are responsible for estimating the optical properties at a given location. Its accurate estimation is essential to plan efficient simulation for radiative transfer. For this sake, synergetic use of reanalysis data with optics database was used as a potential tool to precisely derive the aerosol model on the basis of the major representative particulates exist within a model grid. In detail, mixing of aerosol types weighted by aerosol optical depth (AOD) components has been developed. This synergetic aerosol model (SAM) is spectrally extended up to $40{\mu}m$. For the major aerosol event cases, SAM showed that the mixed aerosol particles were totally different from the typical standard aerosol models provided by the radiative transfer model. The correlation among the derived aerosol optical properties along with ground-based observation data has also been compared. The current results will help to improve the radiative transfer model simulation under the real atmospheric environment.

Considerations and Alternative Approaches to the Estimation of Local Abundance of Legally Protected Species, the Fiddler Crab, Austruca lactea (법정보호종, 흰발농게(Austruca lactea) 서식 개체수 추정에 대한 검토와 대안)

  • Yoo, Jae-Won;Kim, Chang-Soo;Park, Mi-Ra;Jeong, Su-Young;Lee, Chae-Lin;Kim, Sungtae;Ahn, Dong-Sik;Lee, Chang-Gun;Han, Donguk;Back, Yonghae;Park, Young Cheol
    • Journal of Wetlands Research
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    • v.23 no.2
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    • pp.122-132
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    • 2021
  • We reviewed the methods employed in Korean tidal flat surveys to measure the local abundance of the endangered wildlife and marine protected species, the fiddler crab, Austruca lactea. A complete census for infinite population is impossible even in a limited habitat within a tidal flat, and density estimates from samples strongly vary due to diverse biological and ecological factors. The habitat boundaries and areas shift with periodicities or rhythmic activities of organisms as well as measurement errors. Hence the local abundance calculated from density and habitat areas should be regarded as transient. This conjecture was valid based on the spatio-temporal variations of the density averages, standard error ranges, and spatial distribution of the crab, A. lactea observed for 3 years (2015-2017) in Songdo tidal flat in Incheon. We proposed the potential habitat areas using the occurrence probability of 50% from logistic regression model, reflecting the importance of habitat conservation value as an alternative to local abundance. The spatial shape of potential habitat predicted from a generalized model would remain constant over time unless the species' critical environmental conditions change rapidly. The species-specific model is expected to be used for the introduction of desired species in future habitat restoration/creation projects.