• 한국수산과학회지
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• 제21권1호
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• pp.50-56
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• 1988

T. japonius의 배양에 유효한 먹이 미생물을 탐색하기 위하여, T. japonicus가 서식하는 tide pool 면장 해수 및 해조류에서 264균주를 분리, 각 균주에 대한 먹이효과 실험을 하였던 결과, 각 생장단계별로 세균종이 달랐다. 즉 nauplius기, copepodite기 및 성체의 각기에 가장 유효한 먹이 세균은 Acinetobacter sp. AG-3뿐이고, Moraxella spp., Flavobacterium spp.는 copepodite기 이후에, Pseudomonas spp.는 성체기의 먹이 효과가 있었다. 이것은 tide pool속의 많은 세균중 극히 일부에 속하며, 먹이 세균의 최적 농도는 $10^6/ml$로 tide pool 평균 생균수 농도에 거의 일치하였다.

• 대한한방부인과학회지
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• 제21권2호
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• pp.203-213
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• 2008

Purpose: This study is to evaluate the efficacy of non pharmacotherapy on dysmenorrhea. Methods: Survey was done through web site and directly searched dysmenorrhea -related articles in journals of Korean medicine Gynecology, published during 1996-2008. Results: 1. All articles reported non pharmacotherapies were significant efficacy in the decrease of dysmenorrhea. 2. The distribution of therapy was acupunture treatment 14 articles and the other treatments 6 articles. 3. The frequency of acupoint was Sanyinjiao(三陰交, SP6) 10 articles, Guanyuan(關元, CV4) 5 articles, Qihai(氣海, CV6) 4 articles and mainly used meridians were Immaek(任脈), Liver Meridian of Foot Gworeum(足厥陰肝經), Spleen Meridian of Foot Taeum(足太陰脾經). 4. The distribution of period for treatment were the most predominant as 7(35%) articles from one day before menstruation to the second day of menstruation. 5. The distribution of duration for treatment were the most predominant as 7(35%) articles in one day. 6. VAS was mainly used in the evaluation of dysmenorrhea and MDQ, MMSL, MVRS were used in premenstrual syndrome. Conclusion: In this study, non pharmacotherapies were effective on dysmenorrhea.

• International Journal of Precision Engineering and Manufacturing
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• 제7권2호
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• pp.30-35
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• 2006

The purpose of this study is to develop a new cuff to improve the accuracy of blood pressure measurement, and to evaluate the performance of the developed system. We added a small bladder to the normal cuff, which we refer to as the double bladder system. The system that we developed for blood pressure measurement was based on the oscillometric method using a double bladder. This system was developed in order to reduce the oscillation noise and to amplify the signal of pure blood pressure. An oscillometric signal database based on the developed system was evaluated according to the ANSI/AAMI/SP10-1992 standard. The correlation coefficients between the cuff of the double bladder and the normal cuff were 0.98 for systolic pressure and 0.94 for diastolic pressure. The mean differences and the standard deviations between the average blood pressure obtained from a mercury manometer and that obtained from an automated sphygmomanometer were -0.7mmHg and 4.9mmHg for systolic, and -1.4mmHg and 5.4mmHg for diastolic pressure. We conclude that the proposed double bladder-based cuff system improves the accuracy of oscillometric blood pressure measurement. The developed system reduces the range of error by about $44{\sim}62%$ for systolic pressure and about $6{\sim}21%$ for diastolic pressure compared to the most recently developed, commercially available sphygmomanometers.

• Animal Systematics, Evolution and Diversity
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• nspc3호
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• pp.45-58
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• 1992

Tetranychoid mites found from Korea until now are 42 species belonging to 12 genera, 2 families as follows: 1 Bryobia japonica Ehara et Yamada, 2. B. praetiosa Koch, 3. B. rubrioculus(Scheuten), 4. Petrobia latens(Muller), 5. Aponychus corpuzae Rimando, 6. A firmianae(Ma et Yuan). 7. Panonychus citri(McGregor), 8. P. ulmi(Koch), 9. Eotetranychus carpini Oudemans, 10. E. hicoriae(McGregor), 11. E. populi(Koch), 12. E. rubiphilus (Reck), 13. E. sexmaculatus (Riley), 14. E. smithi Pritchard et Baker, 15. E. tiliarium (Hermann), 16. E. uchidai Ehara, 17. Schizotetranychus bambusae Reck, 18. S. celarius(Banks), 19. S. leguminosus Ehara, 20. Oligonychus aceris(Shimer), 21. O. clavatus(Ehara), 22. O. hondoensis(Ehara), 23. O. ilicis(McGregor), 24. O. karamatus(Ehara), 25. O. orthius Rimando, 26. O. peridtus Pritchard et Baker, 27. O. shinkajii Ehara, 28. O. pustulosus (Ehara), 29. O. ununguis(Jacobi), 30. O. sp. 31. Tetranychus kanzawai Kishida, 32. T. phaselus Ehara, 33. T. truncatus Ehara, 34. T. urticae Koch, 35. T. vienensis Zacher, 36. Aegyptobia nothus Pritchard et Baker, 37. Pentamerismus taxi (Haller), 38. P. oregonensis McGregor, 39. Brevipalpus californicus(Banks), 40. B. lewisi McGregor, 41. B. obovatus Donnadieu, 42. Tenuipalpus zhizhilashviliae Reck. On the above species, a taxanomic key was made and ecological data including distribution and host plant are presented in this paper.

• Journal of Microbiology and Biotechnology
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• 제21권9호
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• pp.885-892
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• 2011

The bacterial communities in the intestinal tracts of earthworm were investigated by culture-dependent and -independent approaches. In total, 72 and 55 pure cultures were isolated from the intestinal tracts of earthworms under aerobic and anaerobic conditions, respectively. Aerobic bacteria were classified as Aeromonas (40%), Bacillus (37%), Photobacterium (10%), Pseudomonas (7%), and Shewanella (6%). Anaerobic bacteria were classified as Aeromonas (52%), Bacillus (27%), Shewanella (12%), Paenibacillus (5%), Clostridium (2%), and Cellulosimicrobium (2%). The dominant microorganisms were Aeromonas and Bacillus species under both aerobic and anaerobic conditions. In all, 39 DNA fragments were identified by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis. Aeromonas sp. was the dominant microorganism in feeds, intestinal tracts, and casts of earthworms. The DGGE band intensity of Aeromonas from feeds, intestinal tracts, and casts of earthworms was 12.8%, 14.7%, and 15.1%, respectively. The other strains identified were Bacillus, Clostridium, Enterobacter, Photobacterium, Pseudomonas, Shewanella, Streptomyces, uncultured Chloroflexi bacterium, and uncultured bacterium. These results suggest that PCR-DGGE analysis was more efficient than the culturedependent approach for the investigation of bacterial diversity and the identification of unculturable microorganisms.

• 한국정밀공학회지
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• 제22권5호
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• pp.181-188
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• 2005

The purpose of this study is to develop the new cuff improving the accuracy of blood pressure measurement, and to evaluate the performance of the developed system. We added a small bladder to the normal cuff which is called the double bladder system. The developed system for blood pressure measurement was based on the oscillometric method using a double bladder. This system was developed in order to reduce the oscillation noise and to amplify the signal of pure blood pressure An oscillometric signal database based on the developed system were evaluated following the standard ANSI/AAMI/SP10-1992. The correlation coefficients between cuff of double bladder and normal cuff were 0.98 for systolic and 0.94 for diastolic. Mean differences and the standard deviations between average blood pressure of mercury sphygmomanometer and automated sphygmomanometer were -0.7mmHg and 4.9mmHg for systolic, and -1.4mmHg and 5.4mmHg for diatolic, respectively. We conclude that the proposed double bladder based cuff system improves the accuracy of the oscillometric blood pressure measurement. The developed system reduces the error range about $44\~62\%$ for systolic and about $6\~21\%$ for diastolic compared to the recently developed commercially available sphygmomanometers.

• Parasites, Hosts and Diseases
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• 제59권4호
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• pp.393-397
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• 2021

Although research conducted in East Asia has uncovered parasite eggs from ancient toilets or cesspits, data accumulated to date needs to be supplemented by more archaeoparasitological studies. We examined a total of 21 soil samples from a toilet-like structure at the Hwajisan site, a Baekje-period royal villa, in present-day Korea. At least 4 species of helminth eggs, i.e., Trichuris trichiura, Ascaris lumbricoides, Clonorchis sinensis, and Trichuris sp. (or Trichuris vulpis) were detected in 3 sediment samples of the structure that was likely a toilet used by Baekje nobles. The eggs of T. trichiura were found in all 3 samples (no. 1, 4, and 5); and A. lumbricoides eggs were detected in 2 samples (no. 4 and 5). C. sinensis and T. vulpis-like eggs were found in no. 5 sample. From the findings of this study, we can suppose that the soil-transmitted helminths were prevalent in ancient Korean people, including the nobles of Baekje Kingdom during the 5th to 7th century.

• Journal of Veterinary Science
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• 제25권4호
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• pp.54.1-54.16
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• 2024

Importance: As one of the main etiologic agents of infectious diseases in pigs, pseudorabies virus (PRV) infections have caused enormous economic losses worldwide. EP0, one of the PRV early proteins (EP) plays a vital role in PRV infections, but the mechanisms are unclear. Objective: This study examined the function of EP0 to provide a direction for its in-depth analysis. Methods: In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells. Results: This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization. Conclusions and Relevance: These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.

• 통합자연과학논문집
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• 제7권2호
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• pp.92-102
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• 2014

The crystal structure of the title compounds with both coumarin and sulfonamide moieties were examined. These two groups have very special for their pharmaceutical and medicinal properties have been determined from single crystal X-ray diffraction data. In the title compound crystallizes in the monoclinic space group C2/c with unit cell dimension a = 28.633(3) ${\AA}$, b= 9.3215(7) ${\AA}$ and c= 24.590(2) ${\AA}$ [alpha & gamma=$90^{\circ}$ beta= $115.976(3)^{\circ}$]. In the structure The S1 atom shows a distorted tetrahedral geometry, with O1-S1-O2 [119.74 $(2)^{\circ}$] and N1-S1-C5 [$105.57(1)^{\circ}$] angles deviating from ideal tetrahedral values are attributed to the Thrope-Ingold effect. The sum of bond angles around N1 ($316.2(1)^{\circ}$) indicates that N1 is in sp2 hybridization. The Pyridine ring adopts boat conformation and pyran rings adopt a sofa conformation. The carboxylate group of atoms were disordered over two positions with site occupancy factors 0.598 (9):0.402 (9). Crystal structure and packing is stabilized by $C-H{\ldots}O$ intra and inter molecular hydrogen bond interactions.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6697-6701
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• 2013

Objective: To explore the inhibiting effect and mechanism of Endostar injection concomitant with cryoablation on lung adenocarcinoma A549 xenografts in nude mice. Materials and Methods: A total of 24 nude mice with subcutaneous xenografts of the A549 cell line were established and divided into 4 groups when the maximal diameters of tumors became 1 cm: control group, Endostar group, cryoablation group and combination group (Endostar concomitant with cryoablation). The nude mice were sacrificed after 21-days treatment, tumour tissues were removed to measure their volume, in situ test of TdT-mediated dUTP nick end labeling (TUNEL) was adopted to determine the cellular apoptosis around freezing injury zones, and immunohistochemical SP test was applied for the detection of micro-vessel density (MVD) and vascular endothelial growth factor (VEGF) expression levels. Results: At 21-days after treatment, the growth velocities of control group, Endostar group, cryoablation group and combination group were $236.7{\pm}51.2%$, $220.0{\pm}30.6%$, $159.5{\pm}29.3%$ and $103.3{\pm}25.5%$ (P<0.01), while cellular apoptosis rates of tumors were $21.7{\pm}2.34%$, ($22.17{\pm}1.47$)%, $38.3{\pm}1.37%$ and $49.2{\pm}1.72%$, (P<0.01), respectively, according to the immunohistochemical test. MVD and VEGF expression levels in the combination group were both lower than in other groups (P<0.01), also being positively related (r=0.925, P<0.01). Conclusions: Endostar can significantly improve the inhibitory effects of cryoablation on xenografts of lung adenocarcinoma A549, and the mechanism is probably associated with its function as an inhibitor of tumour neo-angiogenesis through down-regulating VEGF expression.