• Title/Summary/Keyword: Sp1-21

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Effect of Monochromatic Light Emitting Diode on the Growth of Four Microalgae Species (Chlorella vulgaris, Nitzschia sp., Phaeodactylum tricornutum, Skeletonema sp.) (미세조류 4종(Chlorella vulgaris, Nitzschia sp., Phaeodactylum tricornutum, Skeletonema sp.)의 성장에 미치는 발광다이오드 단일파장의 영향)

  • Oh, Seok-Jin;Kwon, Hyeong-Kyu;Jeon, Jin-Young;Yang, Han-Seob
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.21 no.1
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    • pp.1-8
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    • 2015
  • We investigated the effect of monochromatic light emitting diode (LED) on the growth of diatoms Nitzschia sp., Phaeodactylum tricornutum, Skeletonema sp. and green algae Chlorella vulgaris. The four microalgae species were cultured under blue LED (450 nm), yellow LED (590 nm), red LED (650 nm) and fluorescent lamp (mixed wavelengths). The maximum growth rates and cell densities of Nitzschia sp., P. tricornutum and Skeletonema sp. were highest under blue LED, followed by fluorescent lamp, red LED and then yellow LED, however those of C. vulgaris were highest under red LED. This result indicates that blue LED is favorable for the growth of diatoms. Thus, the growth of microalgae under monochromatic light might be species-specific or taxon-specific. Also, these results could be used as an important information in future for remediation of heavy metal contamination in the sediments using LED and microalgae.

THE GROWTH OF FOOD ORGANISMS FOR THE MASS PRODUCTION OF MOLLUSCAN SEEDLINGS 1. Phaeodactylum, Platymonas and Chlorella (패류 종묘의 대 생산시에 필요한 먹이 생물의 배양에 관한 연구 1. Phaeodactylum, Platymonas 및 Chlorella)

  • Yoo Sung Kyoo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.1
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    • pp.1-6
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    • 1970
  • A series of experiments have been made on the mass culture of Phaeodactylum tricornutum, Platymonas sp. and Chlorella sp. in the laboratory. The shortest lag phase was found In the culture of Ph. tricornutum followed by Platymonas sp. and Chlorella sp. As compared to the aeration culture, the stagnant culture, in general, showed long duration of the lag phase, short period of the exponential phase and extremely small daily increment. The relative growth constants of Ph. tricornutum, Chlorella sp. and Platymonas sp. were $0.302{\pm}0.028$, $0.226{\pm}0.013$, and $0.151{\pm}0.008$, respectively The maximum daily increment of the three species and then daily ages are as follows: Ph. tricornutum Maximum daily increment : 47.5, Daily age : 10, Platymonas sp. Maximum daily increment : 5.6, Daily age : 14, Chlorella sp. Maximum daily increment : 21.1, Daily age : 14 Comparing the packed cell volume with a certain number of cells, the largest value was found in the population of Ph. tricornutum followed by Platymenas sp. and Chlorella sp. A straight line relationship exists between the two values, and the magnitude of the relationship coincides well with the size of the cells. The culture of Ph. tricornutum was proved satisfactory for feeding the larvae of bivalves at about 12 days after innoculation and both of Platymenas sp. and Chlorella sp. were about 16 days respectively.

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Betulinic Acid, a Naturally Occurring Triterpene found in the Bark of the White Birch Tree induces Apoptotic Cell Death in KB Cervical Cancer Cells through Specificity Protein 1 and its Downstream

  • Shin, Ji-Ae;Choi, Eun-Sun;Jung, Ji-Youn;Cho, Nam-Pyo;Cho, Sung-Doe
    • Journal of Food Hygiene and Safety
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    • v.26 no.2
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    • pp.150-153
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    • 2011
  • Betulinic acid (BA), a naturally occurring triterpene found in the bark of the white birch tree, has been investigated to induce apoptosis in various cancer cells and animal models. However, there is no report of the chemopreventive effect of BA in cervical cancer cells. Using KB human cervical cancer cells as a model, we currently show that BA decreases cell viability and induces apoptotic cell death. The mechanism of the BA-induced anti-growth response in KB cells is due to the down-regulation of specificity protein 1 (Sp 1) and its downstream targets, myeloid cell leukemia-1(Mcl-1) and survivin. Thus, BA acts as a novel chemopreventive agent through the regulation of Sp1 that is highly expressed in tumors.

The effects of Acupuncture at Taep'o(SP21) on Serum glucose and Urine glucose in Diabetic patient (대포혈(大包穴) 자침(刺鍼)이 당뇨병 환자의 혈당 및 뇨당에 미치는 영향)

  • Park, Jai-Young;Yoo, Chang-Kil;Han, Jai-Seop;Kim, Hyung-Seok;Lee, Young-Hoon;Park, Hee-Soo
    • Journal of Acupuncture Research
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    • v.19 no.1
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    • pp.1-10
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    • 2002
  • Objective : This study was designed to evaluate the possibility of treatment of Diabetes Mellitus by the Acupuncture. Methods : We reviewed 8 patients of Diabetes Mellitus who were diagnosed CVA, low back pain, knee joint pain, etc. They were hospitalized at Sangji University Oriental Medical Hospital during 2001.3.22~2001.10.22. First, we divided into two groups. Group I was administrated by acupuncture at Taep'o(SP21), and was not given any western medicine about Diabetes Mellitus after admission. Group II was administrated by Western medicine. We observed the change of serum glucose(FBS/PP2hrs), urine glucose figure after acupuncture at Taep'o(SP21) for 2 weeks, and compared Group I with Group II. Results : The results obtained as follows ; 1. There was a improvement in Group I(62.5%) and Group II (87.5%). 2. The figure of FBS in Group I was decreased, but there was no signification. There was a significant decrease in Group II (P<0.05). 3. The figure of PP2hrs in Group I was showed a significant decrease(P<0.05). There was also a significant decrease in Group II(P<0.05). 4. The urine glucose of Group I was showed a non-significant increase. There was a significant decrease in Group II(0.05

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Diversity and Plant Growth-Promotion of Endophytic Fungi Isolated from the Roots of Plants in Dokdo Islands (독도의 자생식물 뿌리에서 분리한 내생진균의 다양성과 생장촉진활성)

  • You, Young-Hyun;Yoon, Hyeok-Jun;Lee, Gil-Seong;Woo, Ju-Ri;Rim, Soon-Ok;Shin, Jae-Ho;Lee, In-Jung;Choo, Yeon-Sik;Kim, Jong-Guk
    • Journal of Life Science
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    • v.21 no.7
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    • pp.992-996
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    • 2011
  • Endophytic fungi were isolated from the roots of plants growing naturally on the island of Dokdo. Plant samples, such as Miscanthus sinensis, Achyranthus japonica and Echinochloa crusgali were isolated from Dongdo, and those such as Honkenya peploides and Artemsia koidzumii were isolated from Seodo. Twenty one strains of endophytic fungi were isolated from these plants. To identify the strains, PCR (polymerase chain reaction) amplification of the partial ITS (Internal Transcribed Spacer) regions was done with universal primers ITS-1 and ITS-4 to determine the nucleotide sequence of the ITS regions. Of the strains isolated from Miscanthus sinensis, 75% were Penicillium sp. and 25% were Aspergillus sp. Fifty five percent of strains isolated from Achyranthus japonica were Penicillium sp., 30% were Aspergillus sp. and 15% were Zygorhynchus sp. Strains isolated from Echinochloa crusgali were Penicillium sp. (50%), Aspergillus sp. (12%), Giberella sp. (13%), Talaromyces sp. (9%) and Umbelopsis sp. (8%). Of the strains isolated from Honkenya peploides, 76% were Penicillium sp. and 24% were Pestalotiopsis sp. Strains isolated from Artemisia koidzumii were Penicillium sp. (81%) and Mucor sp. (19%). As a result of bioassay, Ec-3-1 strain isolated from Echinochloa crusgalli showed plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp. was the most abundantly distributed fungal strain in all plants used in this study.

Four New Species of the Genus Hyattella (Dictyoceratida: Spongiidae) from Korea

  • Sim, Chung Ja;Lee, Kyung Jin
    • Animal Systematics, Evolution and Diversity
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    • v.30 no.1
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    • pp.16-21
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    • 2014
  • Four new species of the genus Hyattella (Dictyoceratida: Spongiidae); H. sinchangensis n. sp., H. mara n. sp., H. chuja n. sp. and H. lendenfeldi n. sp., were collected from Jeollanam-do and Jeju-do, Korea. Hyattella sinchangensis n. sp., H. mara n. sp., H. chuja n. sp. and H. grobosa Lendenfeld, 1889 are very similar to each other in some characters. However, Hyattella sinchangensis n. sp. characterized by no special dermal lamella and no tertiary fibres, and more thin secondary fibres than H. globosa. Hyattella mara n. sp. is differs in having well developed dermal lamella, slightly fasciculated primary fibres, and thick tertiary fibres. Hyattella chuja n. sp. differs in surface characters with a slightly elevated part, skeleton with abundant spongine, and very complex meshes. Hyattella lendenfeldi n. sp. is similar to H. tenella (Lendenfeld, 1889) in skeletal structure, thickness of primary and secondary fibres, but differs in growth form.

Germination of Some Fungal Spores under Different Soil Conditions (토양조건(土壤條件)에 따른 수종(數種) 진균포자(眞菌胞子)의 발아(發芽))

  • Shim, Jae-Ouk;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.16 no.1
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    • pp.41-48
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    • 1988
  • To investigate germination characteristics of soils on Fusarium species under different soil conditions, this study was carried out to test spore germination of 4 Fusarium species. Among 102 soil samples, spore germinations of Fusarium solani f. sp. pisi, F. oxysporum f. sp. cucumerium, F. oxysporum f. sp. raphani and F. coccophilum were under 50% in 98, 85, 82 and 83 soil samples, respectively. The highest spore germination of F. solani f. sp. pisi, F. oxysporum f. sp. cucumerium and F. coccophilum was obtained in 21 soil samples of pH 5.1-6.0, whereas F. oxysporum f. sp. raphani was in 33 soil samples of pH 4.1-5.0. The highest spore germination of F. solani f. sp. pisi, F. coccophilum and F. oxysporum f. sp. cucumerium was obtained in 4 soil samples holding soil moisture content of 41-60%, while F. oxysporum f. sp. raphani was in 22 soil samples of 21-30%. F. oxysporum f. sp. raphani only showed a direct correlation(r=0.29) between spore germination and soil moisture content. On the basis of each spore germination in cultivated and non-cultivated soils, there was significant difference(p=0.01) in 4 Fusarium species. F. solani f. sp. pisi only indicated significant difference(p=0.0l) between two contrasting-soils, cultivated and non-cultivated soils. The numbers of microbial population were higher in soils suppressive to spore germination than soils conducive to spore germination.

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Studies on the Rheology of the Biopolymer produced by Bacillus sp. K-1 Strain and its Mutants (Bacillus sp. K-1과 그의 변이주가 생산하는 Biopolymer의 물성에 관한 연구)

  • Kim, Sung-Ho;Yim, Moo-Hyun;Choung, Nack-Hyun
    • Applied Biological Chemistry
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    • v.39 no.3
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    • pp.165-171
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    • 1996
  • The rheological properties of biopolymers produced by Bacillus sp. K-1 and its mutant strains(KM-21, KM-83) were studied. Apparent viscosity of biopolymers decreased as rising the temperature. The biopolymer produced by KM-21 strain showed 1.7 times low viscosity, 2.7 times low viscosity by K-1 and 1.9 times low viscosity by KM-83 at $80^{\circ}C$ compared with at $20^{\circ}C$ respectively. The viscosity of biopolymer solution also increased with increasing the polymer concentration and showed pseudoplastic characteristics which is high temperature dependency in all concentration. The change of the biopolymer viscosity on the pH showed the highest value at the pH 7.0 and it showed lower at acidic condition than at alkaline condition comparatively.

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Characterization and Identification of Bacteria from Putrefying Soybean Curd (부패하는 두부로부터 미생물의 분리ㆍ동정 및 특성조사)

  • 주길재;허상선;최용희;이인구
    • Food Science and Preservation
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    • v.5 no.3
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    • pp.292-298
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    • 1998
  • The isolates from putrefying soybean curd were identified as Acinetobacter calcoaceticus, Bacillus cereus, Bacillus sp., Cardiobacterium sp., Escherichia coli, Klebsiella pneumoniae, Pantoea sp., Salmonella typhimurium, Staphylococcus aureus, Xenorhabdus luminescens, Yersinia sp.. The existence percentages of the bacteria from putrefying soybean curd at room temperature storage were Bacillus cereus J55 23.57%, Xenorhabdus luminescens J48 22.73%, Acinetobacter calcoaceticus J61 22.26%, Klebsiella pneumoniae J62 21.25%, Salmonella typhimurium J51 2.87%, Pantoea sp. J57 2.65%, Bacillus sp. J58 1.43%, Cardiobacterium sp. J54 1.26%, Escherichia coli J53 1.20%, Staphvlococcus aureus J6O 0.93%, Yersinia sp. J50 0.05%, respectively. Four out of eleven bacteria as B. cereu J55, X. luminescens J48, Ac. calcoaceticus J61, Kl. pneumoniae J62 putrefied soybean curd and those bacteria produce amylase or proteinase as a extracellular enzyme. But S. typhimurium J51, Pantoea sp. J57, Bacillus sp. J58, Cardiobacterium sp. J54, E. coli 153, St. aureus J60, Yersinia sp. J50 were not putrefied soybean curd. The isolates detected to resistant on various antimicrobial agents. The majority were resistant to aminoside antiboitics as amicacin, gentamicin, tobramycin and were susceptible to ${\beta}$-lactamine antibiotics as penicillin G, oxacillin, cephalothin cefazolin, cefamandole.

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