The present experiment was conducted to investigate the effects of seeding date on agronomic characters including seed weight of soybean (Glycine max (L.) Merr.) at Sunchon, the southern coastal area of Korea. One hundred eighteen native and improved varieties were used in this study. As the seeding date was delayed, the number of days to flowering for the cultivars was reduced. This trend was more obvious in late maturing cultivars(LMC) than in early and medium maturing cultivars (EMC and MMC). Late seeding also resulted in decrease in the number of leaves, stem length, and number of nodes. The heaviest seed weight was obtained with EMC and MMC planted on May, and seed weight decreased with delayed seeding date. Seed weight was positively correlated with number of days to flowering, number of total leaves at flowering, stem length and number of nodes on main stem at maturity. Based on seed weight the cultivars was classified into five types: Type I; Seed weight of the cultivars decreases with delayed seeding date. Type II; Seed weight of the cultivars does not vary with seeding date. Tyep III; Seed weight of the cultivars increases with delayed seeding date. Type IV; Seed weight of the cultivars increases when the seeding date approached the appropriate seeding date, but decreases thereafter. Type V; The reversed type IV. Type I, II, III, IV and V occupied 37, 16, 17, 10 and 20% of the tested cultivars, respectively.
An attempt was made to survey soybean cultivars that were nonsensitive to the photoperiod for selection as possible breeding materials for wide area adaptable varieties. Fifty-five cultivars were subjected to different day length conditions. The number of days from sowing until flowering was shortened under short day length conditions, and late varieties tended to be more sensitive to day length than early varieties. The response to day length of the tested cultivars might be divided into three groups; low, intermediate and high degree, by their accelerated rate of flowering. Especially four varieties in the low responding group, Gembokin, Wirth, Wayne and Pi.54613 were identified to be nonsensitive to day lengths, that is, neutral varieties. They also showed little variation in morphological characteristics under different day length conditions.
To clarify the effects of sink demand for assimilate on leaf photosynthetic rate, tissue composition, and leaf senescence of soybean [Glycine max (L.) Merr.] J plants, pod and leaf tissues were removed at growth stage $R^3$. Plant responses were measured every 10days from 2 through 42days following treatment. Leaves of depodded plants exhibited increased starch and chlorophyll contents and specific leaf weight. Stomatal resistance was also increased and leaf photosynthetic rate was reduced. Dry weight of vegetative tissues except leaves was increased by pod removal. Leaf removal resulted in a decreased starch content of leaves from 22 to 42days after treatment and that of roots at all sampling times. Specific leaf weight was decreased while leaf photosynthetic rate was increased. Stomatal resistance and chlorophyll content were little affected. Weight per seed was decreased 3.0% by leaf removal. Except for the seed, tissue protein contents were increased by pod removal but decreased by leaf removal, however, seed protein content was not affected by either. Apparent senescence was delayed by depodding. Both apparent and functional senescence were accelerated by leaf removal.
The objective of this study was to optimize the mixing ratio of mixed bean protein concentrate (MBPC) and to improve the quality of sausage analogues. Soybean (Glycine max MERR), mung bean (Phaseolus radiatus L.), red bean [Vigna angularis (Wild.)], and pea (Pisum sativum L.) were mixed and processed to produce a MBPC, which was used to make a sausage analogue. The protein, moisture, and carbohydrate content were significantly (p<0.05) different among the samples. A significant (p<0.05) improvement was observed in textural properties (hardness, gumminess, and chewiness), cooking loss, frying loss, and emulsion stability of the sausage analogue. This study suggested the possibility of attaining high-quality sausage analogues and partial sausage analogues using MBPC, which could serve as a potential ingredient in meat analogues.
Soybean [$Glycine$$max$ (L.) Merr.] protein is a high quality source for food and feed. But, antinutritional factors in the raw mature soybean are exist. Kunitz trypsin inhibitor (KTI) protein is a main antinutritional factor in soybean seed. Also, P34 protein, referred as $Gly$$m$ Bd 30K, has been identified as a predominant immunodominant allergen. Genetic relationship between KTI protein and P34 protein could be useful in soybean breeding program for the genetic elimination or reduction of these factors. The objective of this study was to determine the independent inheritance or linkage between KTI protein and P34 protein in soybean seed. A total of 479 $F_2$ seeds were obtained from the cross of 07B1 and PI567476 parents. KTI protein and relative amount of P34 protein were analysed from $F_2$ seeds harvested from the F1 plants by using SDS-PAGE and Western blot analysis. The segregation ratios of 3 : 1 for KTI protein (353 KTI protein present : 126 KTI protein absent) and relative amount of P34 protein (363 normal amount of P34 protein : 116 low amount of P34 protein). The segregation ratio of 3 : 1 suggested that KTI protein and relative amount of P34 protein in mature soybean seed were controlled by a single major gene. The segregation ratios of 9 : 3 : 3 : 1 (266 KTI protein present, normal amount of P34 protein: 88 KTI protein present, low amount of P34 protein: 102 KTI protein absent, normal amount of P34 protein: 23 KTI protein absent, low amount of P34 protein) and Chi-square value (${\chi}^2$=3.31, P=0.346) were observed in $F_2$ seeds. This data showed that KTI protein was inherited independently with relative amount of P34 protein in soybean. These results will be helpful in breeding program for selecting the line with lacking KTI protein and reduced amount of P34 protein in soybean.
Acetolactate synthase activity inhibition and herbicidal activities were investigated with 2 sulfonylureas [chlorsulfuron{2-chloro-N-{{(4-methoxy-6-methyl-1,3,5-triazin-2-yl) amino} carboxyl} benzenesulfonamide}, metsulfuron-methyl{methyl-2{{{{(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino}carbonyl}amino}sulfonyl}benzoic acid}, and 2 imidazoli-nones [imazethapyr{2-{4,5-dihydro-4-methyl-4-(1-methyl)-5-oxo-1H-imidazol-2-yl}-5-ethyl-3-pyridinecarboxylicacid}, imazaquin{2-{4,5-dihydro-4-methyl-4-(1-methyl)-5-oxo-1H-imidazol-2-yl}-3-quinoline carboxylic acid} herbicides. A broad weeding spectrum was observed with the treated herbicides at low application rates. Both corn(Zea mays L.) and sorghum(Sorghum bicolor Moench) were very sensitive to the two herbicide groups. Although legumes, such as soybean(Glycine max Merr.), clover(Trifolium repense L.), and indian jointvetch(Aeschnomene indica L.) were sensitive to the sulfonylureas, they were tolerant to the imidazolinones. On the contrary, wheat(Triticum aestivum L.) and barley(Hoderum sativum Jess.) showed the reverse responses of the legumes to the two herbicide groups. Quackgrass(Agropyron repens(L.) P. Beauv.). however, was commonly tolerant to the two herbicide groups. Degrees of crop injury and acetolactate synthase inhibition also varied with the crops examined. The 50% inhibition concentrations of sulfonylureas on acetolactate synthase in vitro activity($IC_{50}$) from corn, wheat, and soybean did not relate to the greenhouse herbicidal activities ($GI_{50}$). With chlorsulfuron, for example, wheat had more than 100 times higher $GI_{50}$ than corn and soybean, but the $IC_{50}$ was 4 to 10 times lower. Similar observation was made with metsulfuron-methyl. However, closer relationships between $IC_{50}$ and $GI_{50}$ were found with the imidazolinones. When imazethapyr was applied, the order of $GI_{50}$ values against com, wheat, and soybean was the same as that of $IC_{50}$.
Park, Gyu-Hwan;Baek, In Youl;Han, Won Young;Kang, Sung Taek;Choung, Myoung Gun;Ko, Jong Min
KOREAN JOURNAL OF CROP SCIENCE
/
v.58
no.4
/
pp.383-387
/
2013
This study was carried out to examine whether the leaf size is likely to be used as a selection criterion for large seed genotype in soybean (Glycine. max (L.) Merr.) breeding program. Two hundred twenty nine soybean germplasms which had collected in Korea, United States, China and Japan were used in this experiment. The area of unifoliate leaf, middle leaflet of first trifoliate and third trifoliate leaf ranged from $3.2cm^2$ to $33.8cm^2$, 9.2 to $29.5cm^2$, and 7.2 to $58.9cm^2$, respectively. One hundred seed weight also showed great variation from 2.7 to 39.0 gram. The average leaf area of unifoliate, middle leaflet of first trifoliate and third trifoliate leaf were $15.7cm^2$, $18.1cm^2$ and $32.7cm^2$, respectively, and that of seed average weight was 17.2 gram per one hundred seed. Significantly positive correlations were observed between seed weight and leaf area of unifoliate (r=$0.80^{**}$), first trifoliate (r=$0.75^{**}$) and third trifoliate (r=$0.67^{**}$), respectively. Both the leaf length and leaf width of unifoliate, middle leaflet of first trifoliate and third trifoliate leaf were significantly positively correlated with seed weight and both the correlations of unifoliate were higher than the other leaves. The correlations of leaf width in soybean leaflet were higher than those of leaf length. Leaf length/width (L/W) ratio of upper leaf was higher than that of lower leaf in the leaf size. Both the leaf area and leaf width of unifoliate leaf are the most suitable predictive characteristics of early selection in related to seed weight for soybean breeding program.
Koh, Hong-Min;Park, Sumin;Kim, Kyung Hye;Kim, Ji Min;Lee, Taeklim;Heo, Jinho;Lee, Ju Seok;Jung, Jinkyo;Kang, Sungteag
KOREAN JOURNAL OF CROP SCIENCE
/
v.63
no.3
/
pp.257-264
/
2018
The recent global climate change induced the drought, flooding, and insect pest outbreaks. These caused the severe damage to crop yield in the domesticated field and occurrence change of insect pest species. The sap-sucking insect pest, aphids are common in soybean [Glycine max (L.) Merr.] and cause serious yield losses. Thus, developing resistance cultivars is promising and efficient strategy to prevent the significant yield losses by aphid and screening germplasm is the essential procedure to achieve this goal. We tried to establish a resistance test indicator for foxglove aphid, Aulacorthum solani (Kaltenbach), in soybean and found that plant damage degree or infested plant damage is most suitable one. Also we screened around 1,200 of soybean germplasm including wild and cultivated species for its resistance to foxglove aphid from the various origins, and 67 soybeans, including PI 366121, showed antixenosis resistance, 31 germplasms among 67 antixenosis germplasms were showed antibiosis to foxglove aphid with non-choice test. The identified foxglove aphid resistant soybean resources showed significantly low rate in survival test. Furthermore, resistance type, (i.e., antibiosis or antixenosis) of each candidate were varied. In this research, we established the screening index for foxglove aphid resistance in soybean, and identified the resistance varieties. This result could be useful resources in breeding for new foxglove aphid resistance soybean cultivars, and provide fundamental information to investigate the resistance mechanism in soybean.
Sung, Mi Kyung;Han, Sung Jin;Seo, Hyung Jin;Choi, Sang Woo;Nam, Sang Hae;Chung, Jong Il
KOREAN JOURNAL OF CROP SCIENCE
/
v.59
no.3
/
pp.319-324
/
2014
Soybean (Glycine max (L.) Merr.) is an important crop for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. But, antinutritional factors in the raw mature soybean are exist. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. The objective of this research is to obtain the information on raffinose and stachyose content according to genotype and environment. A total of twenty two soybean genotypes (11 cultivars, 3 germplasms and 8 breeding lines) were selected. Each genotype was grown in the field for two years with two replications and harvested in bulk at natural maturity for two years. Content of raffinose and stachyose was detected by HPLC. The raffinose content (g/kg) of 22 genotypes was $2.68{\pm}0.21-5.87{\pm}2.43$ in year 1 and was $3.24{\pm}0.37-9.05{\pm}0.16$ in year 2. The stachyose content (g/kg) was $4.23{\pm}0.98-27.68{\pm}9.90$ at year 1 and was $5.11{\pm}1.09-25.32{\pm}0.35$ in year 2. Genotype and environment have highly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyose content at 5% significant level in two years. A positive correlation ($R^2=0.1985^*$) between raffinose and stachyose was observed in year 2. These informations are valuable in soybean genetics and breeding program related with raffinose and stachyose content.
In order to establish a optimum level and proper method of fly ash application for soybean cultivation, the successive three years experiment was conducted in the field applied with four application levels of fly ash, 0, 30, 60, 90 MT/ha during the 1991 to 1993. Influence of successive application and residue of fly ash in soil on soybean growth and yield was discussed. Fly ash application had a favorable effect on soybean growth, however over application such as 90 MT/ha caused to turn the color into the brown of young leaf edge and eventually to have necrosis on the leaf. This symptom was prominent under the application of bituminous coal fly ash. In the 1st year cultivation of soybean, the highest yield was obtained at application level of 30 MT/ha. In the 2nd year, application of anthracite fly ash showed the highest yield at 60 MT/ha for successive application and at 90 MT/ha for the 1st year application followed by the 2nd year residue. Application of bituminous coal fly ash showed the highest yield at 60 MT/ha for the both successive application and residue. In the 3rd year, successive application of the both fly ash was given the highest yield at 30 MT/ha, respectively indicating the decrease of yield with increasing level of application. In case of residue plot, the highest yield by the application of anthracite fly ash was made at 90 MT/ha for the 1st year application followed by 2 years residue and at 60 MT/ha for the 1st and 2nd year application followed by the 3rd year residue. But in the residue plot of bituminous coal fly ash, yield was highest at 30 MT/ha showing the decrease of yield with increasing level of residue. Enhancement in growth and yield of soybean by application of fly ash was due to the fact that fly ash contained some plant nutrients such as phosphorus, silicon, and boron etc. and reformed soil pH that caused to increase availability of nutrients in soil.
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