• Journal of Nutrition and Health
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• 제49권2호
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• pp.88-98
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• 2016

본 연구에서는 20대 초반 한국 여성의 짠맛 역치 및 선호도를 측정하여 음식섭취빈도법을 통해 추정한 나트륨 섭취량과의 연관성을 분석하고, 자기 평가 짠맛 선호도와 관련되어 있는 나트륨 섭취 관련 식행동과 나트륨 섭취 기여 음식을 조사하였다. 자기 평가 짠맛 선호도는 음식섭취빈도법을 이용하여 추정한 나트륨 섭취량과 유의한 양의 상관관계에 있었으며, 나트륨 증가 식행동과 양의 상관관계에 있었고, 나트륨 감소 식행동과 음의 상관관계에 있었다. 나트륨 섭취와 관련된 식행동 중에는 식탁에서 소금과 양념간장의 사용, 외식과 배달음식의 빈도, 국, 찌개, 국수류의 국물 섭취, 레토르트나 가공 식품의 이용, 생채소의 섭취, 싱거운 맛에 대한 수용태도 등의 항목이 자기 평가 짠맛 선호도와 유의한 관련성이 있었으며 나트륨 섭취 기여음식 중에는 라면, 순두부찌개, 총각김치 및 김치를 이용한 음식 등이 자기 평가 짠맛 선호도와 유의하게 관련되어 있어, 짠맛 선호도가 이들 식행동 및 음식을 통해 높은 나트륨 섭취와 관련되어 있는 것으로 생각된다. 예상과는 달리 짠맛 검출 및 인식 역치는 자기 평가 짠맛 선호도, 나트륨 섭취량, 나트륨 증가 및 감소 식행동과 유의한 상관관계를 보이지 않아 식품체계가 아닌 염화나트륨 수용액을 이용한 짠맛 역치보다는 자기 평가된 짠맛 선호도가 개인의 평상시 나트륨 섭취량을 간접적으로 평가할 수 있는 더 좋은 지표로서 생각된다. 결론적으로, 자기 평가 짠맛 선호도는 나트륨 섭취와 관련된 식행동과의 연관성으로 인하여 평상시 나트륨 섭취 정도를 잘 반영하는 것으로 나타났으며, 개인의 나트륨 섭취 수준을 평가하는 간단하고 효과적인 도구로서 활용될 수 있을 것이다.

• 분석과학
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• 제21권6호
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• pp.543-552
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• 2008

식품 중 클로로프로판올 화합물인 3-MCPD (3-monochloropropane-1,2-diol, $C_3H_7ClO_2$, MW. 110) 및 1,3-DCP (1,3-dichloropropan-2-ol, $C_3H_6Cl_2O$, MW. 128)를 분석하는 효과적인 방법을 확립하였다. 시료를 5M NaCl용액으로 균질화 한 후 알루미늄옥사이드와 섞어 유리컬럼에 충진하고 디클로로메탄으로 시료 중 클로로프로판올 화합물을 용출하였다. 용출된 클로로프로판올 화합물은 감압증류장치와 질소가스로 농축한 뒤 HFBA (Heptafluorobutyric anhydride, $C_8F_{14}O_3$, MW. 410)로 유도체화하여 GC/MS로 분석하였다. 3-MCPD-HFBA 유도체화 화합물(MW. 502)은 m/z 253, 275, 289, 291, 453를 선택이온으로 하고 1,3-DCP-HFBA 유도체화 화합물(MW. 325)은 110, 275, 277를 선택이온으로 설정하여 정성 정량 하였다. 확립된 분석법의 정량한계는 3-MCPD 및 1,3-DCP 모두 0.01 mg/kg이었고, 0.01~1.00 mg/kg의 농도범위에서 직선성($R^2{\geq}0.999$)이 좋았으며 평균회수율은 약 97%내외였다. 확립된 분석법을 이용하여 다양한 식품 중 클로로프로판올 화합물을 조사한 결과, 0.0~0.3 mg/kg (n=56/157) 수준으로 3-MCPD로 검출되었다.

• 동아시아식생활학회지
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• 제21권1호
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• pp.98-107
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• 2011

This study was conducted via survey of 300 enrolled male and female high school students in the Chungnam area. The survey focused on students' dietary habits, changes in dietary habits based on the school lunch menu, and satisfaction of school lunches, in order to identify new methods for changing dietary habits and improving satisfaction of school lunches. Regarding the general characteristics of those surveyed, most were 17 years old, and of the same gender. Ninety-nine percent of those surveyed lived in farming areas, and the majority of students' parents graduated from high school and are self-employed. Research on dietary habits according to gender found that male students ate more regularly than did female students, with a difference of (p<0.001) between the two genders. Regarding the degree of which students ate only what they want, differences of (p<0.05) were found between the genders. The types of snacks frequently consumed were found to be flour-based snacks for the male students, whereas the females mostly consumed sweet foods and bread snacks. Research on satisfaction of school lunches according to gender also showed a difference of (p<0.5). Specifically, 48.7% of male students responded 'average', whereas only 45.3% of female students responded in the same way. The reason for dissatisfaction of school lunches were quality and nutrition for the male students (30%) and taste for the female students (60.4%), with a significant difference according to gender (p<0.05). Regarding the types of food most left over, both female and male students answered fish, bean curd, and soy products.

• Toxicological Research
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• 제31권3호
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• pp.299-305
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• 2015

An HPLC analytical method was validated for the quantitative determination of biogenic amines in agricultural products. Four agricultural foods, including apple juice, Juk, corn oil and peanut butter, were selected as food matrices based on their water and fat contents (i.e., non-fatty liquid, non-fatty solid, fatty liquid and fatty solid, respectively). The precision, accuracy, recovery, limit of detection (LOD) and quantification (LOQ) were determined to test the validity of an HPLC procedure for the determination of biogenic amines, including tryptamine, ${\beta}$-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, in each matrix. The LODs and LOQs for the biogenic amines were within the range of 0.01~0.10 mg/kg and 0.02~0.31 mg/kg, respectively. The relative standard deviation (RSD) of intraday for biogenic amine concentrations ranged from 1.86 to 5.95%, whereas the RSD of interday ranged from 2.08 to 5.96%. Of the matrices spiked with biogenic amines, corn oil with tyramine and Juk with putrescine exhibited the least accuracy of 84.85% and recovery rate of 89.63%, respectively, at the lowest concentration (10 mg/kg). Therefore, the validation results fulfilled AOAC criteria and recommendations. Subsequently, the method was applied to the analysis of biogenic amines in fermented agricultural products for a total dietary survey in Korea. Although the results revealed that Korean traditional soy sauce and Doenjang contained relatively high levels of histamine, the amounts are of no concern if these fermented agricultural products serve as condiments.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.815-822
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• 2005

In order to extend the shelf-life of packaged or coated foods, an antibacterial edible film was developed from soybean meal that had been fermented with Bacillus subtilis under the optimum condition of pH 7.0-7.5 and $33^{\circ}C$ for 33 h. The water vapor permeability of the fermented film ($86.0 mg/cm^2{\cdot}h$) was higher than those of normal soybean films ($66.9 mg/cm^2{\cdot}h$). Protein solubility of the fermented film was also higher than ordinary soy protein film at the pH range of 3 -10. The fermented soybean film had higher tensile strength and lower $\%$ elongation (elongation rate) than the ordinary soybean film, mainly because partial hydrolysis of proteins in the soybean film occurred during fermentation. Antimicrobial properties of the fermented film on foodstuffs were measured by placing the films on surime, jerked beef, and mashed sausage media; containing $10^2-10^3$ CFU/plate of foodborne pathogenic bacteria, and showed significantly higher inhibitory effects on the growths of all the indicating bacteria. The film could be used as a packaging material in the food industry. However, before direct application of the fermented film to the commercial food industry, its poor mechanical and antibacterial properties need to be improved.

• 한국식생활문화학회지
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• 제12권5호
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• pp.509-517
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• 1997

The purpose of this study is to establish the one serving size of Korean Indigeneous Food. Serving size is necessary to make Nutrition Labeling which is required to export Korean food product especially to the United States of America. The basic data of 100 food items were collected through searching traditional and recent cookbooks. 4 industrial foodservices as noncommercial foodservice and 30 traditional ethnic restaurants and 12 gourmet restaurants in hotels as commercial foodservlce were explored to collect the data of actual serving size of each items. Moreover, experimental cooking and sensory evaluation by trained panels were conducted to assess quantity preference of selected food items. All data were rearranged through food type, that is, main dish, side dish, dessert and health food. One serving sizes showed wide variety according to the different menus that include selected food items. Therefore, means and ranges of serving size by four research methods were presented item by item. There were wide differences in intakes of main dishes, for example, noodles were around $50{\sim}100g$, cereals were 20 g, which means the one serving size can be differenciated by the food usage. In intakes of side dishes, average of side dishes were $20{\sim}30g$, but Kimches, the first traditional Korean food, were $30{\sim}50g$, and the other condiments, pepper paste and soy paste were $5{\sim}10g$. About desserts, liquid types were around 200 g, the other sugars were $10{\sim}20g$, the kind of teas were almost $2{\sim}3g$. The health foods-many kinds of that were Ginseng-were averaged 20 g; but dried mushrooms were around 2 g.

• 한국식생활문화학회지
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• 제35권5호
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• pp.467-477
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• 2020

Doenjang is a major fermented soy-based food in Korea. Recent investigations have shown that fermented soybean foods have immunity-enhancing, anti-cancer, anti-obesity and anti-diabetic effects. Several studies also have reported that genistein and daidzein, which are easily absorbed in the body are produced in larger quantities in aged doenjang. The purpose of this study was to evaluate the variations in the anti-cancer effects of commercialized doenjang as it ages. Four groups were formed for this study according to aging periods of doenjang, namely short (under 5 years, S group), mid (under 10 years, M group), long (under 15 years, L group) and very long (over 15 years, E group). The anti-cancer effects of doenjang were determined by cell cytotoxicity assays in A549, YAC-1, and HepG2 cancer cell lines. Also, NK cell activity and splenocyte proliferation were assayed for cancer immunotherapy. The quantities of phenolic compounds in doenjang at different ages were also measured. The results showed that the anti-cancer effects increased in the S and M groups for all three cancer cell lines. Interestingly, similar to this result, splenocyte proliferation and NK activity were also the highest in the S and M groups. In contrast, the E group showed significantly reduced splenocyte proliferation. The quantity of phenolic compounds was similar to that of the anti-cancer results. Collectively, these results suggest that the fermentation period of doenjang plays a very important role in determining its anti-cancer effects.

• 한국미생물·생명공학회지
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• 제45권2호
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• pp.143-148
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• 2017

메주와 된장에서 분리한 Bacillus 속 126균주를 대상으로 amine oxidase 활성을 나타낸 10균주를 분리하였다. 그 중, B. licheniformis 8MI05와 8MS03 균주는 동일한 염기서열을 가진 amine oxidase 유전자(yobN)를 보유하고 있었다. 공개된 B. licheniformis 유전체 정보를 대상으로 yobN 유전자의 보유를 검색한 결과, yobN 유전자 보유는 균주 특이적인 특성으로 확인되었다. 두 균주 모두 4종류 바이오제닉아민(cadaverine, histamine, putrescine, tyramine) 분해 활성을 나타냈고, yobN 유전자를 보유한 재조합균주에서도 바이오제닉아민 분해 활성이 확인되었다. Amine oxidase 유전자 보유 균주들은 14% NaCl 농도에서 생육 가능하였고, 균주 특이적인 protease 및 lipase 활성을 나타내었다.

• KSBB Journal
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• 제25권2호
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• pp.137-141
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• 2010

신맛이 요구되는 식품에 일반적으로 첨가되는 구연산의 산미 기능과 첨가물로써 물성을 향상시키기 위하여 식물성 유지를 이용하여 코팅 수율이 95%이상이며 코팅물질함량은 총 조성물 중 20~33% 내외인 코팅구연산 제조공정을 개발하였다. 개발된 코팅구연산 (coated citric acid)은 식품에 투입되었을 때 최종제품에서 본 식품의 물성을 변화시키지 않고 구연산 본래의 신맛을 감소시키며, 제품이 입에서 녹을 시점에서 자극적이지 않은 신맛을 서서히 방출 시키는 특징을 기대할 수 있다. 본 연구를 통하여 개발된 코팅 구연산은 식품산업 및 향장품 등 다양한 관련 산업분야적용이 가능함으로써, 고부가가치 제품으로 수입대체 및 해외수출 등 관련 산업 분야로의 파급효과가 클 것으로 기대된다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제10권2호
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• pp.117-128
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• 2007

Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE mediated hypersensitivity disorder, which is associated with mainly gastrointestinal symptoms and has a delayed onset. The vomiting and/or diarrheal symptoms of FPIES typically begin in the first month of life in association with a failure to thrive, metabolic acidosis, and shock. Therefore, the differential diagnosis of FPIES and neonatal or infantile sepsis-like illnesses or gastroenteritis is difficult. The early recognition of indexes of suspicion for FPIES may help in the diagnosis and treatment of this disorder. The diagnosis of FPIES is generally made through clinical practice and food-specific IgE test findings are typically negative in this condition. Therefore, oral cow's milk challenge (OCC) remains the valid diagnostic standard for FPIES. An investigation of positive OCC outcomes helps to find out a diagnostic algorithm of criteria of a positive challenge in FPIES. Moreover, it has not been clearly determined in infantile FPIES when $1^{st}$ follow up-oral food challenge (FU-OFC) should be performed, with what kind of food protein (e.g., cow's milk, soy), and how much protein should be administered. Hence, to prevent the risk of inappropriate FU-OFC or accidental exposure and achieve appropriate dietary management, it is necessary to identify tolerance rates to major foods under the careful follow up of infantile FPIES patients. On the other hand, small intestinal enteropathy with villous atrophy is observed in FPIES and this enteropathy seems to be in part induced by both of epithelial apoptosis and intercellular junctional complex breakdown. The purpose of this report is to introduce an update on diagnostic and therapeutic approaches in FPIES and suggest the possible histopathological evidences in this disorder.