• BMB Reports
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• v.34 no.4
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• pp.316-321
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• 2001

Dehydroascorbate (DHA) reductase (DHAR, EC 1.8.5.1) catalyzes the reduction of DHA to reduced ascorbate (AsA) using glutathione (GSH) as the electron donor in order to maintain an appropriate level of ascorbate in plant cells. To analyze the physiological role of DHAR in environmental stress adaptation, we developed transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants that express a human DHAR gene isolated from the human fetal liver cDNA library in the chloroplasts. We also investigated the DHAR activity, levels of ascorbate, and GSH. Two transgenic plants were successfully developed by Agrobacterium-mediated transformation and were confirmed by PCR and Southern blot analysis. DHAR activity and AsA content in mature leaves of transgenic plants were approximately 1.41 and 1.95 times higher than in the non-transgenic (NT) plants, respectively In addition, the content of oxidized glutathione (GSSG) in transgenic plants was approximately 2.95 times higher than in the NT plants. The ratios of AsA to DHA and GSSG to GSH were changed by overexpression of DHAR, as expected, even though the total content of ascorbate and glutathione was not significantly changed. When tobacco leaf discs were subjected to methyl viologen at $5\;{\mu}M$, $T_0$ transgenic plants showed about a 50% reduction in membrane damage compared to the NT plants.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.3
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• pp.31-54
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• 2019

The purpose of this study is to present the basic data for establishing the management plan of forest ecosystem in Honam-Jeongmeak and Geumnamhonam-Jeongmaek by surveying and analysing the vascular plants distributed in Jangansan(A-site), Palgongsan(B-site), Mandeoksan(C-site), Chuwolsan (D-site), Cheonunsan(E-site) and Jeamsan(F-site). The numbers of flora were 813 taxa including 115 families, 406 genera, 700 species, 4 subspecies, 94 varieties and 15 forms. There were 500 taxa in A-site, 427 taxa in B-site, 402 taxa in C-site, 449 taxa in D-site, 345 taxa in E-site and 524 taxa in F-site. The rare plants were 12 taxa including Paeonia japonica, Iris minutoaurea, Chloranthus fortunei and so forth. In IUCN Red List categories, there were 1 taxon of EN, 2 taxa of VU, 8 taxa of LC and 1 taxon of DD. The Korean endemic plants were 20 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Saussurea pseudogracilis and so forth. The specific plants by floristic region were 108 taxa including 58 taxa of grade I, 30 taxa of grade II, 16 taxa of grade III, 2 taxa of grade IV and grade V, respectively. The plants adaptable to climate change were 18 taxa including 11 taxa of southern plant and 7 taxa of northern plants. The naturalized plants were 49 taxa including Rumex nipponicus, Brassica juncea, Trifolium repens and so forth. The invasive alien plants were 4 taxa including Rumex acetocella, Solanum carolinense, Ambrosia artemisiifolia and Aster pilosus.

• Journal of Life Science
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• v.31 no.6
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• pp.537-542
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• 2021

Schisandra nigra Max., a dioecious plant native to Jeju Island in Korea, is cultivated on a small scale for fruit production. As fruit-producing female individuals are generally considered to be more valuable than male, early identification of plant sex at the seedling stage is important. In this study, a sequence-characterized amplified region (SCAR) marker associated with a female-specific region in the genome of S. nigra was investigated. Of 120 randomly amplified polymorphic DNA (RAPD) primers, one primer (OPB-03) consistently amplified a 749 bp band in female plants. The female-specific PCR product was isolated and cloned, and the nucleotide sequences were then determined. Southern hybridization performed using the female-specific fragment as a probe produced positive signals only in genomic DNA from the female plants. This result revealed that the 749 bp segment of DNA was present in the genome of female plants but absent in the genome of male plants. A SCAR primer pair was designed based on the RAPD marker to amplify a 436 bp fragment in the genomic DNA of female plants. This primer pair amplified the expected size of DNA fragment in female plants and four monoecious individuals collected from a natural population. The SCAR marker identified in this study can be used to distinguish female-flowering individuals at the seedling stage.

• Journal of the Korean Professional Engineers Association
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• v.38 no.6
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• pp.1-9
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• 2005

The Republic of Korea (South) and the Peaples Republic of Korea (North) had agreed to exploit the North Korean rich natural resources starting from the year of 2006. Through this mutual agreement DPRK shall quarantee ROK's investment in the North Korean Mines and supply mineral products for the compasation of the financial investment supported by ROK. In the area of northern part of Korea, many kind of natural resources such as Magnesite, Iron, Gold & Silver, Copper, Lead & Zinc, and Coal are plentifully deposited. In the area of southern part of Korea, however, most kind of natural resources have been exhausted and so presently major minerals such as Gold & Silver, Copper, Lead & Zinc and Coal are being imported and relying on foreign countries in whole quantity of the requirement. On the other hand Northern Part of Korea is making very slow progress in mining and exploitation owing to the lack of investment even though there are rich deposits of natural resources. And in Southern Part of Korea, they have most advanced production facilities and technologies in the world in the fields of Mineral Floatation, Steel Manufacturing, Nonferrous Metal Smelting & Refining and those plants fabrications. A combination of Southern Technologies together with Financial Support and Northern rich natural resources & Labour Power will be a most hopeful, desirable and mutually required 'South-North economic Cooperation' as the mining industries are labour intensive.

• The Korean Journal of Ecology
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• v.17 no.4
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• pp.513-521
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• 1994

Distribution patern of 30 species that are occurring predominantly in the mantle communities (Mantelgesellschaften) in South Korea was studied. The study was arried out by geographic and bioclimatic analysis on 368 releves obtained from the Zurich-Montpellier School's method, which involves direct analysis on the latitude, altitude, annual mean temperature and the lowest temperature of the site. Rosa multiflora and Pueraria thunbergiana which are regarded as repersentative pioneer species to the mantle community has the highest frequency, 70.1% and 60.3%, respectively. Three distribution patterns were recognized, i.e. northern type, central type and southern type, and each type was characterized by horizontal and altitudinal amplitude. Their concetrate distribution ranges on the annual mean temperature were 8~11℃, 9~12℃ and 10~13℃, respectively. It was recognized that tendencies of overlapping and continuous distribution pattern of the types and species exist. Geographically, the souther limit f the northern type is 35.5。N and the northern limit of the southern type 37.0。N. The central type is located at an coincided with the previous study in which cool-temperate forests were synchorologically indentified into northern/altimontane, certral/montane and southern/submontane type. The subsidiary knowledges from this study will provide practical information on the constructuin of the fence plant community for environmental conservation.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.2 no.2
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• pp.135-138
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• 1998

This area is plant-geographically located at the Temperate Subtropical Zone and Evergreen Broad Leaf Zone which is included in the sea of southern area of the Korean Peninsula. The Japanese black pine is the most common tree in this area. The sorts of trees that are living in this area Camellia, Alder, Oak, Acorn. It is reported that this area is located at the Evergreen Broad Leaf Zone of Korean Peninsula that is living in the southern sea. The construction place was originally a grassy place. About 40 familly 100 species different plants were living at this area. The most common animals were the Rodents and the most common birds were the Colombiformes.

• Journal of The Korean Association For Science Education
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• v.15 no.2
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• pp.158-163
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• 1995

The 40th Annual Southern Arizona Regional Science and Engineering Fair was analyzed in terms of contents of exhibits and research methods. Elementary school students like to choose first, biology; secondly. physics; thirdly, chemistry; fourthly. consumer science; fifthly, S.T.S; sixthly. earth science area topics. Chemistry area topics are 20 %, which are much more than rates appeared in American elementary science textbooks. Elementary school students like topics such as pollutions, energy saving materials and characteristics/selection of products required in every-day life. In the most preferential area, biology, students do experiments or survey about plants growth, microbiology, learning/behavior of animals, health/exercise, which are reconcile with the analysis of American elementary science contents.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.563-566
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• 2012

To investigate the possibility of horizontal gene transfer between agricultural microorganisms and soil microorganisms in the environment, Bacillus subtilis KB producing iturin and the PGPR recombinant strain Pseudomonas fluorescens MX1 were used as model microorganisms. The soil samples of cucumber or tomato plants cultivated in pots and the greenhouse for a six month period were investigated by PCR, real-time PCR, Southern hybridization, and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting. Our data from Southern blotting and T-RFLP patterns suggest that the model bacteria do not give significant impacts on the other bacteria in the pots and greenhouse during cultivation.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.87-90
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• 2001

BcGRl gene encoding cytosolic glutathione reductase of Chinese cabbage (Brassica campestris var. Pekinensis cv. Seoul) was placed under the control of the CaMV 35S promoter and introduced into tobacco (Nicotiana tabacum L. cv. Samsun) via Agrobacterium-mediated transformation. T$_{0}$ 32 independent plants transformed with BcGRl gene were selected with kanamycin and they were confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Northern blot analysis revealed that the constitutive expression of BcGRl gene and there was no relationship between the copy number of introduced gene and the levels of BcGRl transcripts.

• Korean Journal of Plant Resources
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• v.21 no.3
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• pp.192-195
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• 2008

This study was conducted to find out some important agronomic characteristics and qualities in response to different transplanting dates in Alisma plantago at southern parts of Korea. Yield components such as number of floral axis per plant, plant height and number of leaves were highest at the transplanting date of Aug. 15 and Aug. 25. Plants sown at Aug. 15 and Aug. 25 also showed highest yield. Considering from our results, optimum transplanting date were semed to be transplanting date of Aug. 15 and Aug. 25.