• The Plant Pathology Journal
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• v.37 no.2
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• pp.200-203
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• 2021

Rice blast caused by the filamentous fungus Magnaporthe oryzae, is arguably the most devastating rice disease worldwide. Development of a high-throughput and reliable field blast resistance evaluation system is essential for resistant germplasm screening, resistance genes identification and resistant varieties breeding. However, the occurrence of rice blast in paddy field is easily affected by various factors, particularly lack of sufficient inoculum, which always leads to the non-uniform occurrence and reduced disease severity. Here, we described a procedure for adequately inducing the occurrence of rice seedling blast in paddy field, which involves pretreatment of diseased straw, initiation of seedling blast for the first batch of spreader population, inducing the occurrence of the second batch of spreader population and test materials. This procedure enables uniform and consistent infection, which facilitates efficient and accurate assessment of seedling blast resistance for diverse rice materials.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.79-79
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• 2022

옥수수(Zea mays L.)의 생육 및 수확량은 온도, 일장, 강수량 등에 의해 영향을 밭는다. 본 연구에서는 남부지역 유휴 논을 이용하여 파종시기를 달리하여 옥수수를 재배하였을 때 생육 특성과 수확량을 분석하여 적정 파종시기를 규명하고자 하였다. 3월 하순에서 6월 중순까지 6차로 구분하여 파종하였을 때 출사일수와 성숙일수는 파종기가 늦어질수록 짧아졌다. 옥수수는 단일 및 고온 조건에 의해 생식생장으로의 전환이 빨라지는데, 본 연구에서도 파종이 늦어짐에 따라 단일과 고온에 의해 출웅과 출사가 촉진되었다. 간장과 착수고는 4월 상순까지는 높으나 파종이 지연됨에 따라 짧아졌다. 옥수수의 수량구성요소 중에 이삭장은 5월 하순까지는 유의적인 차이가 없으나 6월에 파종하였을 때에는 짧아졌으며, 이삭직경과 착립장도 파종이 늦어짐에 따라 가늘어지고 짧아져, 파종이 지연되었을 때 생육이 부진함을 알 수 있다. 이삭의 수확량은 4월 상순까지 파종하였을 때에는 800kg/10a 이상으로 높으나, 파종시기가 늦어짐에 따라 점차 감소하여 6월 중순에는 40% 이상 크게 감소하였다. 이는 수량구성요소 중에 이삭장, 이삭직경과 착립장 등 이삭 발달의 부진한 결과이다. 그리고 이삭을 제거한 지상부의 무게는 5월 하순까지의 파종에서는 유의적인 차이가 없으나 6월에는 큰 폭으로 감소하였는데, 이는 영양생장 기간이 짧아짐에 따른 결과로 보인다. 이상의 결과를 토대로 남부지역 논에서 옥수수를 재배할 때에는 늦어도 5월 중·하순까지는 파종하여야 할 것으로 판단된다.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.87-90
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• 2001

BcGRl gene encoding cytosolic glutathione reductase of Chinese cabbage (Brassica campestris var. Pekinensis cv. Seoul) was placed under the control of the CaMV 35S promoter and introduced into tobacco (Nicotiana tabacum L. cv. Samsun) via Agrobacterium-mediated transformation. T$_{0}$ 32 independent plants transformed with BcGRl gene were selected with kanamycin and they were confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Northern blot analysis revealed that the constitutive expression of BcGRl gene and there was no relationship between the copy number of introduced gene and the levels of BcGRl transcripts.

• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.189-195
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• 1996

In order to obtain basic information for breeding varieties of onion which is suitable at the southern part of Korea. 222 early mature variety and 219 late mature variety were grown from sep.1993 to jun. 1994 at experimental field of Muan-Gun area, and yield components and yield of onion plant ware observed. Early mature onion cv. Changnyeongchunghwan-gkeukjoseng and Keumjungjoseng showed higher yield than other early mature variety used in the experiment. It showed relatively long plant height and leaf sheath length and, height of bulb and diameter of bulb and high weight of bulb. Late mature onion cv. Chunjudaego showed higher yield than other late mature variety used in the experiment. It showed relatively long plant height and leaf sheath length and diameter of bulb, and high weight of bulb. Therefore, it was concluded that Changnyeongchunghwangkeukjoseng,Keumjungjoseng and Chunjudaego were the most suitable early and late mature varieties with high yield at the southern part of Korea.

• Korean Journal of Breeding Science
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• v.49 no.3
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• pp.224-229
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• 2017

A new peanut variety 'Daan'(Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2014. This was developed from the cross between 'Iksan 31' with Virginia typed short stem and 'Dakwang' with Shinpung-typed larger grain. 'Daan' which is a Shinpung plant type had 13 branches per plant and its length of main stem was 44cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 34 pods per plant, 127 g of 100-seed-weight and 75% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 47.8% of crude oil and 28.3% of protein content. This variety showed resistant to early leaf spot and had more resistant to late leaf spot, stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years 'Daan' was more productive than reference variety by 16% with 5.00 MT/ha for grain production.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.240-246
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• 1998

A cDNA encoding desiccation-related protein was isolated from a flower bud cDNA library of Chinese cabbage (C-DH) and its nucleotide sequence was characterized. It contains 679 bp nucleotides with 501 bp open reading frame. The amino acid sequence of the putative protein showed the highest amino acid sequence homology (79 % identity) to dehydrin protein in Gossypium hirsutum. Also, the C-DH shares 48-52% amino acid sequence identity with the other typical dehydrin proteins in plant cells. When the amino acid sequence of their proteins were aligned, several peptide motifs were well conserved, of which function has to be solved. Particularly the C-DH contains 15 additional amino acids at its N-terminus. Genomic Southern blot analysis using the coding region of C-DH showed that the C-DH consists of a single copy gene in Chinese cabbage genome. The C-DH mRNA, whose transcript size is 0.7 kb, was expressed with a tissue-specific manner. It was highly expressed in seed, flower buds and low expression as detected in root, stem or leaf tissues of Chinese cabbage. And the transcript level of C-DH was significantly induced by the treatment of plant hormone, abscisic acid and water-deficit conditions.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.55-58
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• 1994

Agrobacterium tumefaciens LABA4404 harboring plant binary vector, pBI121, was used for genetic transformation of lettuce (Lactuca sativa t.). Cotyledon segments were infected with A. tumefaciens LBA4404 by cocultivation method and regenerated. Regenerated letture was subject to molecular analyses for integration into plant nuclear genome and expression of ${\beta}$-glucumnidase (GUS) gene. Southern and Northern blot analyses demonstrated that GUS gene was integrated into plant nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by specetrophotometric assay of GUS activity.

• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.188-194
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• 1998

This experiment was conducted to introduce phosphinothricin acetyl -transferase(PAT) gene, resistant to basta and non-selective herbidide, into tobacco(Nicotiana tabacum cv.BY4). For shoot formation,tobacco leaf disks were placed on the MS medium supplemented with 2.0mg/L BA and 0.1mg/L NAA. In this medium condition, tobacco leaf disces were cocultivated with A. tumefaciens MP90 containing NPT IIand PAT resistant to kanamycin and Basta, respectively. Shoots were obtained in the medium containing antibiotics, and those were transferred to rooting medium supplemented with 0.1mg/L NAA and antibiotics. The plants obtaining roots were transplanted into soil. Phenotype of transgenic tobacco plant was mostly as normal plant. However, about 5% was abnormal plant, which did not set seeds. PCR analysis and southern blot were performed to determine transformation. As the results, it was confirmed that PAT gene was stably integrated into tobacco genome.When herbicide, basta, was sprayed to the plants confirmed by PCR, the transgenic plants showed normal growth, whereas normal plants died. Therefore, the result of this experiment show that tobacco transformation for the resistance to basta, non-selective herbicide, was successful because PAT gene was stably integrated into tobacco.

• Korean Journal of Plant Taxonomy
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• v.48 no.3
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• pp.218-229
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• 2018

Myanmar's plant diversity is expected to be very high given the wide variety of climates and the diverse vegetation and geographical features of the country. Since the publication of Kress et al.'s plant checklist in 2003, new and unrecorded species have been constantly reported by various botanists, but much of Myanmar's flora requires more intensive examinations. We conducted joint floristic surveys of several Ywangan areas, including the Panlaung-Pyadalin Cave Wildlife Sanctuary in southern Shan State of Myanmar. The initial identification of seed plant specimens collected from three short floristic expeditions revealed that 23 species were newly recorded species in Myanmar. More than half of these were found to be geographically notable species, which are known to be endemic to neighboring countries such as China (4 spp.), Thailand (6 spp.), and India (2 spp.). A considerable number of these unrecorded species are distributed in the limestone areas of neighboring countries, reflecting the geological characteristics of the survey area. The results of this study reemphasize the need for intensive and continuous research on the flora of Myanmar for a more comprehensive understanding of the distribution patterns of flowering plants in Southeast Asia.

• KEPCO Journal on Electric Power and Energy
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• v.6 no.4
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• pp.483-484
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• 2020