• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.323-327
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• 1998

The flavonoid biosynthetic pathway has been studied as a genetic model system, particularly in Petunia hybrida. In order to study the flavonoid biosynthetic pathway, we constructed a fusion gene system between Cauliflower Mosaic Virus (CaMV) 35S promoter and eggplant flavonoid 3', 5'-hydroxylase in pBI 121 plasmid. An optimal condition for plant regeneration was observed when internode explants were cultured on MS medium supplemented with IAA 0.2 mg/L plus BA 3 mg/L. For plant transformation internode explants of Petunia hybrida were precultured on BM medium supplemented with IAA 0.2 mg/L plus BA 3 mg/L. Putative transgenic plants were selected on medium containing kanamycin 50 mg/L plus cefotaxim 300 mg/L. Putative selected transformants were confirmed by amplification of selectable marker gene (nptII) by polymerase chain reaction (PCR) and Southern hybridization of flavonoid 3',5'-hydroxylase gene.

• Plant Resources
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• v.7 no.1
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• pp.39-46
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• 2004

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.64-64
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• 2023

콩은 중요한 식량 작물로 단백질, 기름 및 이차대사산물인 이소플라본을 다량으로 함유하고 있다. 이소플라본은 폴리페놀의 일종으로 일부 암을 예방하며 심혈관 질환을 완화하는 데에도 도움을 준다고 알려져 있다. 콩에 인위적으로 식물호르몬인 ABA를 처리할 때 이차대사산물인 kaempferol의 함량이 증가한다고 보고 되었다. 따라서 이 연구는 식물 생장조정제 처리가 콩잎의 이소플라본 함량에 미치는 영향을 알아보기 위해 수행되었다. 연구는 밀양 남부작물부 유리온실에서 수행되었으며, 콩(품종:선풍)은 와그너포트 1/2000에 1주 2본 파종하여 재배하였다. Ethephone(500, 1000, 2000 ppm)과 ABA(100, 200, 400 ppm)를 사용하였으며 각각 R2, R5, R7기에 처리하였다. 처리 후 잎을 5일 간격으로 3회 채취하여 이소플라본 6종과 쿠메스트롤의 함량을 분석하였다. 초고성능 액체 크로마토그래피(Acquity UPLC H-Class system, Waters)를 사용하여 분석하였다. 이소플라본 함량은 R5 단계에서 전반적으로 높게 나타났으며, R2 및 R7 단계에서는 무처리와 유사한 수준을 보였다. ABA를 처리하였을 때 생장조정제 간의 차이가 에테폰보다 더 큰 것으로 나타났다. 쿠메스트롤 함량은 처리시간에 따라 R7, R5, R2 순으로 높은 것으로 확인되었으며, 처리시간이 지날수록 함량이 증가함을 알 수 있었다. 생장조정제 처리에 쿠메스트롤 함량은 ABA보다 에세폰 처리 시 더 높은 경향을 보였다.

• Korean Journal of Plant Taxonomy
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• v.44 no.3
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• pp.208-221
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• 2014

The Holocene Hypsithermal Interval(or climatic optimum) was the warmest post-glacial period: temperatures rose to as much as $1-4^{\circ}C$ above present temperatures. We hypothesize that southern plants expanded northward and upward during the Hypsithermal Interval. The Hypsithermal relict populations are defined as populations which distribute mainly in Is. Cheju and Southern subregions and have populations of short-distance dispersion separated by more than 100 km from the nearest distribution. The Hypsithermal relict species were identified and their distributions were mapped based on botanical floras and checklist, herbaria specimens, ecological studies, and field surveys of selected species. Evaluation of the hypothesis was based on reviews of published pollen stratigraphic and paleoecological studies. The results showed that the Holocene Hypsithermal relicts such as Corylopsis coreana Uyeki, Carpinus turczaninowii Hance, Stewartia koreana Nakai ex Rehder were expanded northward during the Holocene Hypsithermal Interval.

• Korean Journal of Plant Resources
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• v.16 no.2
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• pp.160-167
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• 2003

In order to investigate the effects of developmental stage of embryos and plant growth regulators on mass production of Zantedeschia spp. Southern Light, immature zygotic embryos of Zantedeschia spp. Southern Light were cultured on Murashige and Skoog(1962) basal media or containing 2,4-D, NAA and BA. Globular embryos did not grow on any of the 2,4-D, NAA and BA combinations. The most suitable stage of immature zygotic embryo culture on the induction callus and multiple shoot was at early cotyledonary embryo stage, and at this stage of embryos were germinated up to 87.5%. The whitish watery callus and yellowish compact nodular callus produced on all 2,4-D, NAA and BA media. The best combination for inducing embryogenic callus was 0.5 mgL NAA and 1.0 mg/L BA. Whitish watery calli have been subcultured for more than 8 months and have retained their producing ability, Plant regeneration was only obtained by direct shoot development and yellowish compact nodular calli. Abundant plantlets were regenerated from cotyledonary stage of embryo culture on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA. Supplementation of the media with 10％ coconut water showed as the best concentration for plant differentiation from direct developed of shoots. The number of regenerated plants from one embryo could be seperated 25-35s plantlets. All yellowish compact callus-derived plantlets were transferred to pots containing a mixture of vermiculite, perlite and sand(1:1;1 v/v) and 100% of divided plantlets were phenotypically normal.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.10a
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• pp.33.2-33
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• 1999

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.10a
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• pp.52.1-52
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• 1999

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.143-149
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• 2003

This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacerium-mediated transformation. Twenty-five transgenic plants T$_{0}$ plants, back cross (BC$_1$) populations to wild type and F$_1$plants between different T$_{0}$ plants were prepared, and polymerase chain reaction(PCR), PCR-Southern blot analysis, and field test with 0.1% Basta treatment were done. The results of PCR, PCR-Southern blot hybridization, and field test indicated that NPTII and bar gene introduced into the genome of petuina plants were stably transmitted to their progenies, and conferred the plants resistance to herbicide, Basta.sta.

• Journal of Plant Biology
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• v.19 no.2
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• pp.49-61
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• 1976

A survey was conducted on the vegetation of Judo and Gamagseum in warm temperate zone of Southern Korea. They are covered with natural vegetation, evergreen forest dominated with Castanopsis cuspiadata in Judo and Machilus thunbergii in Gamagseum. Judo is a small island, ca. 1.75 ha, located within the Wando port. Since the flora of Wando including the Judo was reported in 1924, some investigator have reported evergreen trees of Judo. But the list of plant species of Judo is still unavailable. Gamagseum, located at the 15km morth of Judo, is a small island, ca. 1.45 ha, cosisting of two islands, Dae-o-do and So-o-do in low tide, and the report of its flora and any other survery on its is almost none. The vegetatons of Judo and Gamagseum are an example of natural forest vegetation occured rarely in warm temperate zone because of human disturbance in the southern coast zone of the Korean Peninsula. However, the ecological study of those vegetation has not ever been made, and the ecological or plant geographical situations of their vegetation is not clear. To determine the vegetation type, listing of plant species in the islands, calculation of basal area of trees over DBH=4.5cm, Raunkiaer's life form, leaf size class, Pte.-Q and etc., were studied. Total plant species of Judo was 110 species and that of Gamagseum was 99 species. In Judo, Castanopsis cuspidata was 1384 individuals among 2359 individuals over DBH=4.5cm, and in Gamagseum, Machilus thunbergii was remarkably abundant and Castanopsis cuspidata could not be found.

• Journal of Korean Society on Water Environment
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• v.22 no.3
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• pp.437-443
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• 2006

The Water usage is relationship which is close with the administrative cost from industrial facility. It is not easy to reduce a water usage. This research is the optimization of the waste water quantity which process waste water integration of the standard thermal power plant in system operate time. The turbine rotates by force of the steam and it produces an electricity. Demineralization Water is manufacture purity manufacturing equipment and it is supplied in power plant channel. We knew a possibility of reducing from pure control process. When it is reduced the Back Washing time, Rinsing time of the gravity filter and the activated carbon filter. Also, It is possible even from regeneration phase in Condensate Polishing Demineralization System. In addition, There is also the water which the drain of the sampling water for watching the condition of power plant process will be able to use. Integrates these processes it will be able to reduce an annual 30,000 ton degree. The research is want to use the fundamental data for the water curtailment of the power plant.