• 식물병연구
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• 제30권2호
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• pp.181-188
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• 2024

Southern blight, caused by the soil-borne fungus Sclerotium rolfsii, is a serious disease that affects many economically important crops. In this study, we selected Bacillus subtilis GJ6-14, from a total of 260 strains, to control Southern blight in pepper plants. In both seedling and plant tests, GJ6-14 significantly suppressed disease incidence and severity compared to control, furthermore, GJ6-14 demonstrated efficient colonization in the rhizosphere by maintaining the population from log 5.41 to log 3.92 in the pathogen-inoculated plants, indicating its potential as a biocontrol agent. Molecular analysis revealed up-regulation of defense-related genes, such as a 7.6-fold increase in LOX1 and 15.5-fold increase in PR1, at 72 hr after inoculation of S. rolfsii in GJ6-14-treated plants, suggesting activation of plant defense mechanisms. Overall, our findings highlight the promising role of B. subtilis GJ6-14 as a potential biocontrol agent in sustainable management of Southern blight in pepper plants.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 춘계학술대회
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• pp.144-144
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• 2018

• The Plant Pathology Journal
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• 제32권5호
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• pp.481-488
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• 2016

Dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. Dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. Dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. The objective of this study was to develop a strategy to effectively control plant disease with reduced use of dithiocarbamtes. Southern corn leaf blight was the model pathosystem for the investigation. When corn plants were drench-treated with Bacillus cereus C1L, a rhizobacterium able to induce systemic resistance in corn plants against southern leaf blight, frequency of spraying dithiocarbamate fungicides could be decreased. The treatment of B. cereus C1L was able to protect maize from southern leaf blight while residues of dithiocarbamates on leaf surface were too low to provide sufficient protection. On the other hand, frequent sprays of mancozeb slightly but significantly reduced growth of corn plants under natural conditions. In contrast, application of B. cereus C1L can significantly promote growth of corn plants whether sprayed with mancozeb or not. Our results provide the information that plant disease can be well controlled by rhizobacteria-mediated induced systemic resistance in combination with reduced but appropriate application of dithiocarbamate fungicides just before a heavy infection period. An appropriate use of rhizobacteria can enhance plant growth and help plants overcome negative effects caused by dithiocarbamates.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 추계학술대회
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• pp.216-216
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• 2018

• The Plant Pathology Journal
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• 제15권5호
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• pp.308-310
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• 1999

A fungus associated with gray blight on tea plant (Camellia sinensis) was identifed as Pestalotiopsis theae based on the mycological characteristics. Mycelial growth on potato dextrose agar and size and shape of conidia of P. theae were similar to those of P. longiseta, but P. theae was different from P. longiseta in the color of three median cells and the number of apical appendages. Artificial inoculation of conidial suspension or mycelial mats on the wounded leaves and shoots of healthy plants induced the same disease, respectively. The Korean native variety was relatively. The Korean native variety was relatively more resistat to P. theae than a Japanese variety‘Yabukita’which has bee recently introduced and planted in large areas of southern parts of Korea. Here, we report the report the first record of gary blight caused by P. theae on tea plant in Korea.

• 한국농림기상학회지
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• 제24권4호
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• pp.305-317
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• 2022

배 화상병의 성공적 방제를 위해 2018년부터 2022년까지 우리나라 중부지방의 주요 발병지와 남부지방의 미발병 주산지 주요지점 25곳에 대한 Maryblyt를 구동하여 꽃감염 위험도를 조사하였다. 최근 5년 중 2019년과 2022년 개화기간 중 꽃감염 위험도가 가장 높았다. 한편, 개화기간 중 최적의 꽃감염 방제 처리는 High 경보 다음날에 방제하고, 강우예보를 발령한 전날 방제하는 처리가 배 꽃감염을 낮추는 것으로 평가하였다. 월동 궤양으로부터 활성화된 궤양이 병징을 보일 것으로 Maryblyt가 예측한 날은 대략 중부지방 기준 5월 중순이었는데 이때부터 현장에서 궤양 모니터링을 개시하도록 권장하였다. 천안, 이천, 상주, 나주 등 4곳의 배 과수원에 설치한 영상자료로부터 배 개화기간을 이론적으로 계산한 값과 실제 관측한 값의 차이점을 비교한 결과 남부지방은 이론치나 실측치보다 늘 빠르게 개화를 예측하므로 재조정이 필요하였다. 향후 현장 관리자와 농민들로부터 과원에서 관측한 기상, 기주인 과수, 병징 출현일 등의 정보들이 축적된다면 발병 예측 모델은 현재보다 더 정확한 정보를 제공할 수 있을 것으로 기대된다.

• 한국식물병리학회지
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• 제13권1호
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• pp.22-29
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• 1997

Etiological and ecopathological studi88888es on the leaf blight of kiwifruit (Chinese gooseberry), a disease newly found in 1993 in Korea, were carried out in this experiment. The leaf blight symptom was prevalent in the southern area of Chonnam Province where kiwifruits were widely cultivated. The disease occurred from July to fall, causing early defoliation of kiwifruit vines. Small brownish circular or irregular lesions appeared initially on the leaves, starting from the marginal leaf areas, and enlarged. Afterwards small grayish black granules (acervuli) were formed on the lesions. The color of old lesions changed to grayish brown or grayish white. The causal fungus was identified as Pestalotiopsis menezesiana (Bress. & Torr.) Bissett according to its morphological characters and pathogenicity (pathogenic to Chinese gooseberry and grapevine, but not to other hosts of Pestalotiopsis spp.such as tea, apple, persimmon, and chestnut). As P. longiseta and P. neglecta were reported recently in Japan as pathogens causing similar blight symptoms in kiwifruit, P. menezesiana is a new leaf blight pathogen in kiwifruit. Development of the disease was stimulated by wounding, prevailing wind, and drought. The causal fungus was proven to be overwintered in the diseased leaves on the ground surface.

• 한국식물병리학회지
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• 제10권1호
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• pp.54-60
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• 1994

Incidence of Rhizoctonia blight ranged from 22.2% to 100% in the golf courses at six geographical locations in Korea from 1989 to 1993. Rhizoctonia blight occurred more severly in southern area than in northern area. Fifty seven isolates of Rhizoctonia solani obtained from diseased parts of zoysiagrasses were grouped to AG2-2 by anastomosis test. Pathogenicity testes revealed that this pathogen was strongly pathogenic to Korean lawngrasses(Zoysia japonica, Z. matrella, Z. tenuifolia), but not pathogenic to creeping bentgrass(Agrostis palustris), bermuldagrass(Cynodon dactylon), Kentucky bluegrass(Poa pratensis), perennial ryegrass(Lolium prenne), and creeping red fescue(Festuca rubra subsp. rubra L.). The isolation frequency of R. solani AG2-2 fro sheaths of the infected plants was the highest by 91.67%, and that from stolons and roots was 11.13% and 5.63% respectively. The pathogen was not isolated from the leaves. Population density of R. solani in the lawn of large circular patch was highest on surface soils down to 1 cm deep with the value of 4.9$\times$104 (CFU/g soil), but below 1 cm population density decreased sharply down to 0.8~9.8$\times$103 (CFU/g soil). Horizontal distribution of propagules in turfgrass soil was higher in the margin than in center of patch, where the number of propagules was similar to these of healthy looking soils close to the margin of diseased patch. The meteorological factors influencing the outbreak of the disease were temperature, the number of rainy days and precipitation. Optimum temperature for disease development of Rhizoctonia blight in field was 20~22$^{\circ}C$, and that for hyphal growth of R. solani AG2-2 in vitro was 25~3$0^{\circ}C$. In Pusan area, Rhizoctonia blight first occurred in late April and rapidly developed in late June. The disease slightly decreased during July to August and developed again in late September in 1993. The monthly disease progress in Pusan area was similar to that in Kyeonggi province.

• 식물병연구
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• 제24권4호
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• pp.265-275
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• 2018

Cashew (Anacardium occidentale L.) is an export crop and source of income in Tanzania. However, its productivity is challenged by insect pests and diseases. Cashew Leaf and Nut Blight Disease (CLNBD) caused by Cryptosporipsis spp. has been cited as one of the most devastating diseases in Tanzania. Studies were conducted to investigate incidences and severities of CLNBD on cashew in farmers' fields and elite cashew hybrids developed in 1996 and 1998 in eastern and southern zones of Tanzania. Furthermore, a screen house experiment was conducted to screen these hybrids against CLNBD at Naliendele Agricultural Research Institute (NARI), Mtwara, Tanzania. The results indicated significant differences (P<0.001) in CLNBD incidences and severities in cashew in farmers' fields across Bagamoyo, Nachingwea and Mtwara districts. Further, there were significant differences (P<0.001) among hybrids in CLNBD severities in the screen house experiment. In ranking the elite cashew hybrids, 38 were tolerant and 14 were susceptible to CLNBD. This observation suggests that elite cashew hybrids developed in 1996 and 1998 are more tolerant to CLNBD compared to cashew found in farmers' fields. These findings strongly suggest that the elite cashew hybrids can be recommended for commercial farming in Tanzania.

• Journal of Forest and Environmental Science
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• 제34권6호
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• pp.481-489
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• 2018

In this study, the succession and stand dimension attributes related to the disaster prevention function of Pinus thunbergii coastal forests were examined after damage from Diplodia tip blight. In 2015, 101 years after the Taisho eruption, field investigations were performed on the vegetation, soil thickness, and pH of surface soil of P. thunbergii coastal forests in western Sakurajima (Hakamagoshi plot) and Taisho lava flows in southeastern Sakurajima (Seto plot). The Hakamagoshi plot had more woody plant species with larger basal areas than that in the Seto plot. The mean age and height, maximal age and height of plant species, and H/D ratio were all larger in the Hakamagoshi plot than in the Seto plot. These results may be explained by the relatively smaller effect of volcanic ash and gas on forests in the Hakamagoshi plot compared to the Seto plot, resulting in a more suitable environment for many plant species. Although P. thunbergii coastal forests in Sakurajima are currently recovering from damages owing to Diplodia tip blight, there has not yet been a sufficient recovery compared to the results from a 1997 study. Furthermore, the results of assessment based on the H/D ratio and abundance of trees in P. thunbergii forests indicate that both regions are not yet effective in disaster prevention. Thus, it is necessary to establish Pinus trees, which can adjust to harsh environments like coastal areas and are resistant to volcanic ash and gas, to enhance the disaster prevention function of P. thunbergii coastal forests in volcanic regions. It may also be helpful to establish coastal forests with ectotrophic mycorrhizal fungi and organic matter coverage. Additionally, it is necessary to ensure the continuous maintenance of stand density and soil quality, and further develop efforts to prevent Diplodia tip blight and promote forest recovery.