• Journal of Microbiology and Biotechnology
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• v.5 no.1
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• pp.6-13
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• 1995

A gene coding for the $\beta$-galactosidase (lactase) of Kluyveromyces tragilis UCD 55-55 was isolated by complementation in Escherichia coli YMC9. From the plasmid library made from Sau3A-digested chromosomal DNA, one positive clone was selected. The cloned gene for $\beta$-galactosidase was on 7.3 kilobase pair DNA fragment, and a slightly low level of $\beta$-galactosidase enzyme activity was detecied in E. coli. It was also confirmed that the cloned gene comes from K. tragilis by DNA-DNA hybridization and immunochemical blotting experiments. In order to construct a new yeast strain having the metabolic ability for lactose, the cloned gene for K. tragilis $\beta$-galactosidase was inserted in yeast vector YEp24 and YRp17, and transformed into Saccharomyces cerevisiae YNN27 and Ml-2B. The yeast transformants showed the nearly the same $\beta$-galactosidase productivity as level of K. tragilis when uninduced, but these could not utilize lactose as a sole carbon source, presumably due to the lack of lactose transport system. Nevertheless, a slightly higher ethanol productivity was achieved by these transformants than S. cerevisiae or K. tragilis, in the medium containing glucose and lactose.

• Journal of Korea Society of Digital Industry and Information Management
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• v.10 no.1
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• pp.55-60
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• 2014

Today, there is increasing the elderly population in rural community, and people of returning from the urban to the rural community are demand to be of high value-added agriculture. In this time, there are required to regularization, standardization, automation, for getting of production of high value crops. In this paper, we are study for automation cultivation control system design for produce high-value crops. this system were designed of two parts that one part is measure and control unit, another part is server part for database and server side control. the main controller for measurement and control is used MC9S08AW60, server for Database and server-side control was using MySQL with CentOS. The source code of control program was coding C and compile with GCC. the functions of measurement and control unit are digital input and output each 8channels and can be scan-able of 20 Bit with 2CH/Sec. Analog Output were designed that can be output of 4-20mA or 0-5V on 4channel. The Digital input and output part were designed 8-channel, and using the high speed photo coupler and relays. We showed that system is possible to measure a 20bit data width, 2Ch/sec as 8 channel analog signals.

• Journal of Microbiology and Biotechnology
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• v.20 no.5
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• pp.853-861
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• 2010

High substrate costs decrease the profitability of polyhydroxyalkanoates (PHAs) production, and thus low-cost carbon substrates coming from agricultural and industrial residuals are tested for the production of these biopolymers. Among them, crude glycerol, formed as a by-product during biodiesel production, seems to be the most promising source of carbon. The object of this study was to characterize the mixed population responsible for the conversion of crude glycerol into PHAs by cultivation-dependent and -independent methods. Enrichment of the microbial community was monitored by applying the Ribosomal Intergenic Spacer Analysis (RISA), and the identification of community members was based on 16S rRNA gene sequencing of cultivable species. Molecular analysis revealed that mixed populations consisted of microorganisms affiliated with four bacterial lineages: ${\alpha}$, ${\gamma}$-Proteobacteria, Actinobacteria, and Bacteroides. Among these, three Pseudomonas strains and Rhodobacter sp. possessed genes coding for polyhydroxyalkanoates synthase. Comparative analysis revealed that most of the microorganisms detected by direct molecular analysis were obtained by the traditional culturing method.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.7A
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• pp.530-539
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• 2009

Wireless Relaying is a promising solutions to overcome the channel impairments and provides high data rate coverage that appear for beyond 3G mobile communications. In this paper we present end to end BER performance of dual hop wireless communication systems equipped with multiple Decode and Forward relays over Rayleigh fading channel with the best relay selection. We compare the BER performance of the best relay with the BER performance of single relay. We select the best relay based on the end to end channel conditions. We further calculate the outage probability of the best relay. It is shown that the outage probability of the best relay is equivalent to the outage probability when all relays take part in the transmission. We apply Orthogonal Space Time Block coding(OSTBC) at the source terminal. Numerical and simulation results are presented to verify our analysis.

• Genomics & Informatics
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• v.2 no.2
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• pp.86-91
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• 2004

Even though it represents $6-13\%$ of human genomic DNA, Alu sequences are rarely found in coding regions. When in exon region, over $80\%$ of them are found in 3' untranslated region (UTR). Pseudogenes are an important component of human genome. Their functions are not clearly known and the mechanism of how they are generated is still debatable. Both the Alu and Pseudogenes are important research problems in molecular biology. mRNA is thought to be a prime source of pseudogene and active research is going on its molecular mechanism. We report, for the first time, that mRNAs containing Alu repeats at 3' UTR has a significantly high correlation with processed pseudogenes, suggesting a possibility that Alu containing mRNAs have a high tendency to become processed pseudogenes. It is known that about $10\%$ of all human genes have been transposed. Transposed genes at 3' UTR without Alu repeat have about two processed pseudogenes per gene on average while we found with statistical significance that a transposed gene with Alu had over three processed Pseudogenes on average. Therefore, we propose Alu repeats as a new and important factor in the generation of pseudogenes.

• Proceedings of the KIEE Conference
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• 2003.07d
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• pp.2540-2542
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• 2003

This paper presents an internet-based self-learning educational system which can be enhancing efficiency in the learning process of Java language. The proposed self-learning educational system is called Java Web Player(JWP), which is a Java application program and is executable through Java Web Start technologies. In this paper, three important sequential learning processes : concept learning process, programming practice process and assessment process are integrated in the proposed JWP using Java Web Start technologies. This JWP enables the learners to achieve efficient and interesting self-learning since the learning process is designed to enhance the multimedia capabilities on the basis of educational technologies. Also, online voice presentation and its related texts together with moving images are synchronized for efficient language learning process. Furthermore, a simple/useful compiler is included in the JWP for providing language practice environment such as coding, editing, executing and debugging Java source files. Finally repeated practice can make the learners to understand easily the key concepts of Java language. Simple multiple choices are given suddenly to the learners while they are studying through the JWP and the test results are displayed on the message box.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.50-55
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• 2014

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

• ALGAE
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• v.21 no.2
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• pp.175-183
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• 2006

Recent phylogenetic studies of scytosiphonacean brown algae show many conflicts with current classification. In order to clearly define the phylogenetic relationships of the family, we newly sequenced the photosystem I coding psaA gene (1488 base pairs) from 13 taxa (15 samples), of the family, and, for comparison, rbcL from four taxa. The psaA region has more informative sites (17.9%) than the rbcL (13.1%) and the number of nodes supported by over 50% bootstrap values is more in the psaA phylogeny (53 /57 nodes; 93%) than in the rbcL (47/63 nodes; 74.6%). The psaA phylogenies are basically congruent with the rbcL trees, recognizing two major groups in the monophyletic Scytosiphonaceae. The first group included Myelophycus, Petalonia, Scytosiphon, and elongate sack-shaped species of Colpomenia, primarily cold temperate elements with unilocular zoidangia on sporophytes. The second group, although not resolved, consisted of Hydroclathrus, Chnoospora, Rosenvingea, and ball-shaped Colpomenia, primarily warm-temperate taxa with both unilocular and plurilocular zoidangia on sporophytes. Chnoospora is not monophyletic, as was previously shown the paraphyly of Colpomenia, Petalonia, and Scytosiphon. Hydroclathrus clathratus from Korea and Japan was not monophyletic. Our studies show that gametophytic characters are the main source of conflict for the present taxonomy of the family. The psaA region is a useful tool for resolution of phylogenetic relationships within the Scytosiphonaceae.

• Journal of Internet Computing and Services
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• v.5 no.2
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• pp.85-95
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• 2004

The web-based project is seriously not efficient area at software engineering. In order to develop a web-based system, UI(User Interface) designers usually first design display layout and then business logic developers bring to complete the coding of the display layout source. In the case of maintenance, it goes through the same process, This kinds of web-based software development method were proposed by the various methods same as the tag library of JSP, But there are many problems to apply them and they are low productive comparing to the CIS based software development method, WFS(Neb Form System), which is suggested on this thesis, separates UI design from business logic on a web project and offers the better environment to develop web based software, Eventually WFS will improve the productivity to develop web based software.

• Proceedings of the Korea Multimedia Society Conference
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• 2004.05a
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• pp.709-713
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• 2004

This Paper Presents an Internet-based Java self-learning educational system which consists of a management system named Java Web Player (JWP) and creative multimedia contents fer Java language. The JWP Is a Java application program free from security problems by the Java Web Start technologies that supports an Integrated learning environment including three Important learning Procedures: Java concept learning Process, Programming practice process and assessment process. This JWP enables the learners to achieve efficient and Interesting self-learning since the learning process is designed to enhance the multimedia capabilities on the basis of various educational technologies. On-line voice presentation and its related texts together with moving images are synchronized for efficiently conveying creative contents to learners. Furthermore, a simple and useful compiler is included in the JWP fur providing user-friendly language practice environment enabling such as coding, editing, executing and debugging Java source files on the Web. The assessment process with various items helps the learners not only to increase their academic capability but also to appreciate their current degree of understanding. Finally, simple multiple choices are given suddenly to the learners while they are studying through the JWP and the test results are displayed on the message box. The proposed system can be used for an efficient tool for learning system on the Web.