• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.20-27
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• 1997

Objectives of this study was to investigate effects of supplemental chromium (Cr) as CrP in growing pigs treated with pST. Seventy two Landrace pigs weighing average 60 kg were alloted to the three treatments during the 52-d experimental period: control (corn-soybean basal diet); pST treatment (4 mg/head/day); pST + CrP treatment (4 mg and 200 ppb/head/day). Upon termination of feeding trial weighing average 105 kg, thirty-six pigs randomly selected from each treatment were slaughtered to compare carcass traits. For the study of lipid metabolism, eighteen pigs were alloted to the same treatments. Adipose tissue samples from eighteen pigs were collected to investigate lipid metabolism. All treated samples with pST and pST + CrP showed improvements in daily weight gain, regardless of sex. Feed/gain ratio significantly improved in pigs treated with pST and pST + CrP. Dressing percentages were higher in pigs treated with pST and pST + Crp. Carcass grades were significantly higher in pigs treated with pST and pST + CrP. Lipolysis of adipose tissue measured in vitro was significantly increased in pigs treated with pST, lipogenesis in vitro showed opposite tendency. Even though the current data does not show synergistic effects on the above parameters when CrP and pST were supplied at the same time, but CrP supplementation tended to improve growth performance and carcass traits of pigs treated with pST.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.290-290
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• 2004

유전자재조합 소 소마토트로핀(recombinant bovine somatotropin, rBST)은 인슐린양성장인자(IGF)-1의 합성과 분비량 조절에 의해 당과 아미노산의 흡수 및 단백질합성의 촉진을 통한 체내흡수 영양소 이용효율 증가, 산유량 증진 및 증체 효과, 세포증식의 촉진작용 등이 있는 것으로 보고(Bauman, 1982; Mears, 1995; 권 등, 1997)된 바 있으며, 또한 최근에 젖소 및 육우빈우의 인공수정시 rBST를 투여하였을 때 1회 수정수태율, 혈중 progesterone 농도 및 IGF-1 농도 등에 영향을 미치는 것으로 보고하였다(Lucy 등, 1994; Bilby 등, 1999; Morales-Roura 등, 2001). (중략)

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.14-14
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• 2002

The purpose of this study was to determine the effect of bST treatment on progesteron concentration, embryo recovery and pregnancy rate following embryo transfer. Donor cows were superovulated with Folltropin-V and PGF₂ α combination method and then inseminated with frozen semen 3 times 12 hrs interval. Donor and recipient cows were assigned to control and bST group, of which was given a single injection of bST (500 ㎎, sc) at insemination or estrus detection. Embryo collection of superovulated cows were flushed nonsurgical method at 7 to 8 days after artificial insemination. (omitted)

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.5
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• pp.732-737
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• 2003

Anti-antibodies against three mouse monoclonal antibodies viz. IIB5D6, VIA6E8 and VIC1F9 (specific to bovine growth hormone) in rabbits have been generated and characterized. Ammonium sulfate fractionated and affinity-purified monoclonal antibodies were used for producing anti-antibodies. The generated anti-antibodies were against common as well as uncommon antigenic determinants present in mouse monoclonal antibodies. The raised anti-antibodies replaced [$I^125$ ]bGH bound to goat liver microsomes indicating production of anti-idiotypic antibodies against bovine growth hormone. These antibodies can have profound implications in vivo in lactating bovines for enhancing milk yield.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.1
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• pp.101-106
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• 1996

This experiment was conducted to investigate the effect of bST administration on growth performance and carcass characteristics of finishing Korean native bulls. Fifty four Korean native bulls of about 14 months of age weighting an average 420 kg were assigned to receive no injection of bST, 250 mg of bST s.c. injection every week, or 500 mg bST s.c. injection every 2 weeks (Control, 250ST, 500ST) in a $3{\times}3$ randomized block design for a period of 20 weeks Animals administered with bST responded with decreased feed DMI by 8%(p < 0.01), increased ADG by 12%(p < 0.05), and increased gain/feed ratio by 20%(p < 0.01). But there was no significant difference in the growth performance between 250ST and 500ST. Administration of bST increased dressing percentage by 1.8%(p < 0.05), and tended to increase longissimus muscle area and decrease backfat thickness and marbling score.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1794-1802
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• 2001

Biotechnology will play critical role in improving animal productivity. Animal growth rate and muscle deposition potential can be greatly improved by the application of biotechnology and biotechnological products. Administration of recombinant somatotropin (ST) or other compounds such as IGF-1 and growth hormone-releasing peptides (GHRPs) can enhance growth rate and carcass lean percentage. Gene transfer offers a powerful approach to manipulate endocrine system and metabolic pathways toward faster growth and better feed efficiency. Biotechnology is also extensively used for improving metabolism and activity of gut microorganisms for better nutrient digestibility. Knockout of growth-inhibiting genes such as myostatin results in considerable acceleration of body weight and muscle growth. Animal growth can also be improved by the use of gene therapy. Immunomodulation is another approach for efficient growth through controlling the activity of endogenous anabolic hormones. All the above aspects will be discussed in this review.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1785-1793
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• 2001

The interaction of growth hormone with it's receptor results in dimerization of receptor, a feature known in action of certain cytokines. The interaction results in generation of number of signalling molecules. The involvement of Janus kinases, mitogen activated kinases, signal transduction and activator of transcription proteins, insulin like substrate, phosphatidylinositol 3-kinase, phospholipase C, protein kinase C is almost established in growth hormone action. There are still many missing links in explaining diversified activities of growth hormone. Amino acid sequence data for growth hormones and growth hormone receptors from a number of species have proved useful in understanding species specific effects of growth hormone. Complete understanding of growth hormone action can have implications in designing drugs for obtaining desired effects of growth hormone.

• Journal of Veterinary Clinics
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• v.29 no.3
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• pp.213-219
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• 2012

The recombinant bovine somatotropin (rbST) has been used for increasing milk production of dairy cows without adverse health effects. This study was conducted to compare effects of supplementation with $Boostin^{(R)}$-250 containing 250 mg of rbST on milk production with those of $Posilac^{(R)}$ and $Boostin^{(R)}$-S. And safety of rbST supplementation on target animals was also observed. Each twenty-five lactating dairy cows were assigned randomly to one of four groups. $Boostin^{(R)}$-250 and vehicle (control) were administered weekly. $Boostin^{(R)}$-S and $Posilac^{(R)}$ were administered two week intervals. Milk yield, milk components, milk somatic cell count, health status, and body condition score of cows were examined. Supplementation with $Posilac^{(R)}$, $Boostin^{(R)}$-S, and $Boostin^{(R)}$-250 induced more milk yield than control group by 2.9 kg/day (12.3%), 4.2 kg/day (17.9%), and 4.1 kg/day (17.4%), respectively. There was a significant difference in milk yield among three rbST treatment groups and control group (${\alpha}$ = 0.05). The rbST supplementation did not increase the incidence of clinical mastitis and milk somatic cell counts. Supplementation with rbST did not significantly affect milk components (milk fat, protein, and solid not fat). The rbST supplementation of the dairy cows after peak milk yield did not cause negative effect on BCS. However, some cows less than 100 days in milking had decreased BCSs after rbST supplementation. In conclusion, milk production in 250 mg of rbST administered cows every week was similar to that of 500 mg of rbST administered cows every 2 weeks. And supplementation of 250 mg of rbST every week could reduce metabolic stress in cows.

• Fisheries and Aquatic Sciences
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• v.11 no.4
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• pp.183-189
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• 2008

In this study, tests were conducted to investigate the effects of three dietary growth hormones, administered in various amounts, on the growth performance and lysozyme activity in juvenile olive flounder, Paralichthys olivaceus. Three dietary growth hormones, recombinant human growth hormone (rHGH), recombinant bovine somatotropin A (rBST A) and recombinant bovine somatotropin B (rBST B) were tested at three different supplemental levels (10, 20 or 40 mg/kg body weight per week) by a $3{\times}3$ factorial design and a complete randomized design in comparison to a control group. Fish were fed one of the ten experimental diets (control, $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{10}$, rBST $B_{20}$ and rBST $B_{40}$) for 6 weeks and afterward were analyzed for growth performance by measuring weight gain (WG), feed efficiency (FE), specific growth rate (SGR) and protein efficiency ratio (PER). Based on the factorial design analysis, fish fed rHGH diets demonstrated significantly higher growth performance than fish fed rBST A or rBST B diets. However there were no significant differences in WG, FE, SGR and PER between fish fed rBST A and rBST B diets. Neither hormone level nor the interaction between the different hormones and their various levels had a significant effect on WG, FE, SGR, PER, lysozyme activity or whole-body proximate composition. A complete randomized design analysis confirmed fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{20}$ and rBST $B_{40}$ diets for 6 weeks showed higher WG than fish fed the control diet (P<0.05). A higher FE was observed in fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{20}$ and rBST $A_{40}$ diets in comparison to fish fed the control diet. Fish fed all graded rHGH, rBST A and rBST B supplemented diets showed a higher SGR than fish fed the control diet. Regarding PER, fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$ and rBST $B_{20}$ diets were higher than fish fed the control diet. Furthermore, the lysozyme activity of fish fed a diet of $rHGH_{20}$ was significantly higher than that of fish fed any other diet. The results measuring the growth and development of the fish clearly suggest the biopotency of dietary rHGH could be higher than those of both dietary rBST A and rBST B. Further implied is the probability that within the range of 10 to 40 mg/kg BW/week the dietary growth hormones could accelerate growth performance, and that 20 mg rHGH/kg BW/week could possibly enhance lysozyme activity in juvenile olive flounder, Paralichthys olivaceus.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.3
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• pp.355-365
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• 2010

The aim of this study was to investigate the effect of supplemental recombinant bovine somatotropin (rbST) and cooling with misters and fans on renal function in relation to regulation of body fluids in different stages of lactation in crossbred Holstein cattle. Ten, 87.5% crossbred Holstein cattle were divided into two groups of 5 animals each, housing in a normal shaded barn (NS) and in a shaded barn with a mister-fans cooling system (MF). The experiment in each group was divided into 3 phases, early- (Day 75 postpartum), mid- (Day 135 postpartum), and late stage of lactation (Day 195 postpartum). The pre-treatment study was conducted on the starting day of each stage of lactation and the treatment study was performed after the end of the pre-treatment, during which the animal was injected with 500 mg of rbST (POSILAC) every 14 days for three times. During the study, ambient temperature at the hottest period daily in the MF barn was significantly lower, while relative humidity was higher than that of the NS barn. The temperature humidity index (THI) in both barns ranged from 79-85 throughout the periods of study. Cows in the MF barn showed a lower rectal temperature and respiration rate as compared with cows in the NS barn. The effect of rbST administration increased both rectal temperature and respiration rates of cows housed in either the NS or MF barn. Milk yield significantly increased in cows treated with rbST in all stages of lactation. Increases in mammary blood flow, accompanied by increases of total body water (TBW), extracellular fluid (ECF), blood volume (BV) and plasma volume (PV), were observed in both groups of cows receiving rbST in all stages of lactation. No alterations of renal blood flow and glomerular filtration rate were observed in cows receiving rbST, but decreases in urinary excretion and fractional excretion of sodium, potassium and chloride ions appeared to correlate with reduction in the rate of urine flow and osmolar clearance during rbST administration. These results suggest that the effect of rbST supplementation to cows housed either in NS or MF barns on body fluid volume expansion is attributable to changes in the rate of electrolyte excretion by the kidney. The increased availability of renal tubular reabsorption of sodium, potassium and chloride ions during rbST treatment was a major factor in retaining body water through its colligative properties in exerting formation of an osmotic force mechanism.