• Title/Summary/Keyword: Solution growth

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Effect of Feeding Bypass Protein with Urea Treated Grass on the Performance of Red Kandhari Calves

  • Wankhede, S.M.;Kalbande, V.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.7
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    • pp.970-973
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    • 2001
  • An experiment was carried out to assess the effect of feeding concentrate mixtures varying in bypass protein levels with urea-treated or untreated grass on the performance of twelve Red Kandhari calves (14 months of age and 78.15 kg body weight) for a period of 75 days. Dry grass was treated with 4 percent urea solution and ensiled for 30 days. The CP ($N{\times}6.25$) content in urea treated grass increased from 3.96 to 8.89 percent. Two iso-caloric and iso-nitrogenous concentrate mixtures (CM-I and CM-II) varying in RDP to UDP ratio viz., 65:35 and 55.45 were prepared. The calves in control group ($T_1$) were fed concentrate mixture-I with ad libitum untreated dry grass and those in experimental group ($T_2$) were fed concentrate mixture-II with ad libitum urea treated dry grass. The dry matter consumption in group $T_2$ was significantly (p<0.01) higher as compared to group $T_1$. The total DMI in $T_1$ and $T_2$ was 146.92 and 166.95 kg respectively, whereas the DMI per day and per 100 kg body weight was 1.94 and 2.22 and 1.90 and 2.35 kg, respectively. The average total gain in body weight (kg) and average daily gain (g) of calves in $T_2$ was significantly (p<0.01) higher as compared to those in $T_1$ the values being 28.66, 18.33 and 382.16, 244.44, respectively. Feed efficiency in terms of kg DM per kg gain in body weight was significantly (p<0.01) lower in group $T_1$ than in $T_2$. The cost of feed per kg gain in body weight for $T_2$ and $T_1$ group was Rs. 21.14, 28.22, respectively. The digestibility coefficients of DM, CP, EE, CF, NFE, NDF and ADF were 59.60, 57.50, 53.00, 65.04, 45.82, 48.48, 52.48 and 55.73 for $T_1$ group. The coressponding values were 68.78, 67.80, 59.83, 71.41, 49.93, 53.37 and 57.81, respectively for $T_2$ group. The digestibility coefficients for all the proximate principles in $T_2$ were significantly (p<0.01) higher as compared to $T_1$. However, NDF and ADF digestibilities were not significantly different. Nutritive value determined in terms of DCP and TDN for The experimental ration was significantly (p<0.01) higher than control ration, the values being 7.32 and 47.34 and 9.39 and 52.40% respectively. The blood urea nitrogen levels at 0, 3 and 6 h interval after feeding were significantly (p<0.01) lower in calves fed experiment ration as compared to control. The overall results indicated that in Red Kandhari calves an optimum growth can be economically achieved by feeding 4 percent urea treated dry and mature grass as basal roughage supplemented with a concentrate mixture containing 20 percent CP, 70% TDN and 45% UDP/bypass protein.

Bioinformatics services for analyzing massive genomic datasets

  • Ko, Gunhwan;Kim, Pan-Gyu;Cho, Youngbum;Jeong, Seongmun;Kim, Jae-Yoon;Kim, Kyoung Hyoun;Lee, Ho-Yeon;Han, Jiyeon;Yu, Namhee;Ham, Seokjin;Jang, Insoon;Kang, Byunghee;Shin, Sunguk;Kim, Lian;Lee, Seung-Won;Nam, Dougu;Kim, Jihyun F.;Kim, Namshin;Kim, Seon-Young;Lee, Sanghyuk;Roh, Tae-Young;Lee, Byungwook
    • Genomics & Informatics
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    • v.18 no.1
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    • pp.8.1-8.10
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    • 2020
  • The explosive growth of next-generation sequencing data has resulted in ultra-large-scale datasets and ensuing computational problems. In Korea, the amount of genomic data has been increasing rapidly in the recent years. Leveraging these big data requires researchers to use large-scale computational resources and analysis pipelines. A promising solution for addressing this computational challenge is cloud computing, where CPUs, memory, storage, and programs are accessible in the form of virtual machines. Here, we present a cloud computing-based system, Bio-Express, that provides user-friendly, cost-effective analysis of massive genomic datasets. Bio-Express is loaded with predefined multi-omics data analysis pipelines, which are divided into genome, transcriptome, epigenome, and metagenome pipelines. Users can employ predefined pipelines or create a new pipeline for analyzing their own omics data. We also developed several web-based services for facilitating downstream analysis of genome data. Bio-Express web service is freely available at https://www. bioexpress.re.kr/.

ATM-induced Radiosensitization in Vitro and in Vivo

  • Choi, E.K.;Ahn, S.D.;Rhee, Y.H.;Chung, H.S.;Ha, S.W.;Song, C.W.;Griffin, R.J.;Park, H.J.
    • Journal of Radiation Protection and Research
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    • v.28 no.3
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    • pp.233-237
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    • 2003
  • It has been known that ATM plays a central role in response of cells to ionizing radiation by enhancing DNA repair. We have investigated the feasibility of increasing radiosensitivity of tumor cells with the use of ATM inhibitors such as caffeine, pentoxifylline and wortmannin. Human colorectal cancer RKO.C cells and RKO-ATM cells (RKO cells overexpressing ATM) were used in the present study. The clonogenic cell survival in vitro indicated that RKO-ATM cells were markdely radioresistant than RKO.C cells. Treatment with 3 mM of caffeine significantly increased the radiosensitivity of cells, particulary the RKO-ATM cells, so that the radiosensitivity of RKO.C cells and RKO-ATM cells were almost similar. The radiation induced G2/M arrest in RKO-ATM cells was noticeably longer than that in RKO.C cells and caffeine treatment significantly reduced the length of the radiation induced G2/M arrest in both RKO.C and RKO-ATM cells. Pentoxifylline and wortmannin were also less effective than caffeine to radiosensitize RKO.C or RKO-ATM cells. However, wortmannin was more effective than caffeine against human lung adenocarcinoma A549 cells indicating the efficacy of ATM inhibitor to increase radiosensitivity is cell line dependent. For in vivo study, RKO.C cells were injected s.c. into the hind-leg of BALB/C-nuslc nude mice, and allowed to grow to 130mm3 tumor. The mice were i.p. injected with caffeine solution or saline and the tumors irradiated with 10 Gy of X-rays. The radiation induced growth delay was markedly increased by 1-2 mg/g of caffeine. It was concluded that caffeine increases radiosensitivity of tumor cells by inhibiting ATM kinase function, thereby inhibiting DNA repair, that occurs during the G2/M arrest after radiation.

Electrochemical Characteristics of Supercapacitor Based on Amorphous Ruthenium Oxide In Aqueous Acidic Medium (비정질 루테늄 산화물을 사용한 수계 Supercapacitor의 전기화학적 특성)

  • Choi, Sang-Jin;Doh, Chil-Hoon;Moon, Seong-In;Yun, Mun-Su;Yug, Gyeong-Chang;Kim, Sang-Gil
    • Journal of the Korean Electrochemical Society
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    • v.5 no.1
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    • pp.21-26
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    • 2002
  • A supercapacitor was developed using an amorphous ruthenium oxide material. The electrode of supercapacitor was prepared using an amorphous ruthenium oxide, which was synthesized from ruthenium trichloide hydrate$(RuCl_3{\cdo5}xH_2O)$. Thin film of tantalum was used as a current collector because it had wide. potential window characteristics than titanium and 575304 materials. A supercapacitor was assembled with ruthenium oxide as an electrode active material and 4.8M sulfuric acid solution as an electrolyte. The specific capacitance of the electrode was tested by a cyclic voltammetry using a half cell. The maximum differential specific capacitances during the oxidative and the reductive scans were 710 and $645\;F/g-RuO_2{\cdot}nH_2O$, respectively. The average specific capacitance was $521\;F/g-RuO_2{\cdot}nH_2O$. The assembled supercapacitor was protonated to the potential level of 0.5V vs. SCE. Super-capacitor, which was adjusted to the appropriate protonation level, had the specific capacitance of $151\;F/g-RuO_2{\cdot}nH_2O$ based on the concept of full cell.

A High Viscosity of Curdlan at Alkaline pH Increases Segregational Resistance of Concrete (염기성 pH에서의 고점도 커들란에 의한 콘크리트의 재료분리 억제 효과 증진)

  • 이인영;김선원;이중헌;김미경;조인성;박영훈
    • KSBB Journal
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    • v.14 no.1
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    • pp.114-118
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    • 1999
  • In order to use a polysaccharide, curdlan, as a concrete admixture, we first developed a pilot-scale fermentation process for the mass production of curdlan. We also examined the rheological properties of curdlan, and tested how well the curdlan obtained in this work increased the segregational resistance of the cement slurry. Fermentation was performed in a 300-liter fermenter equipped with 3 disk-turbine impellers. Since curdlan production is stimulated under nitrogen-limiting conditions, the culture pH was shifted from the optimal pH for cell growth (pH 7.0) to the optimal pH for curdlan production (pH 5.5) at the onset of ammonium exhaustion. We obtained a curdlan production of 65 g/L in 120 hr batch cultivation of Agrobacterium species. The insoluble curdlan at the final stage of fermentation was readily harvested by centrifugation together with the cells. The freeze-dried sample contained 78% (w/w) of curdlan. The solubility and viscosity of the curdlan increased with the increase of the solution pH, which enhances the viscosity of concrete since the pH of concrete is extremely high (pH 13.0). Test results of the curdlan as a concrete admixture with cement slurry demonstrated that it prohibits the leakage of water. In conclusion, this work certifies and enlarges curdlan's industrial potential as a concrete admixture.

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Pile Load Transition and Ground Behaviour due to Development of Tunnel Volume Loss under Grouped pile in Sand (사질토 지반에서 터널체적손실 증가에 따른 군말뚝의 하중변이와 지반거동)

  • Oh, Dong Wook;Lee, Yong Joo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.37 no.2
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    • pp.485-495
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    • 2017
  • A development of underground space is very useful solution to slove problem occurred from ground surface enlargement in urban areas due to the growth of population, tunnelling is the most popular way and widely used. Researches regarding tunneling-induced pile-soil interactive behaviour have been conducted by many researchers. A study on pile axial force distribution due to tunnelling through laboratory model test, however, is being rarely carried out. In this study, therefore, authors investigate ground behaviour due to tunnelling below grouped pile subjected vertical load as well as pile axial force distribution. A concept of volume loss is used to express tunnel excavation, which is normally applied to 1~2% for tunnelling in soft ground. In this study, however, 10% of that applied to investigate failure mechanism. As a result of laboratory model test, a decrease of pile axial force occurs at 1.5% of volume loss, settlement of grouped pile is 1.2~4.7 times greater than the adjacent ground surface one. Ground deformations at 1.5% of volume loss are measured using Close Range Photogrammetry and compared with results from numerical analysis.

Herbicidal Property and Soil Behavior of a New Herbicide, Azimsulfuron (신제초제(新除草劑) Azimsulfuron의 제초활성(除草活性)과 토양중(土壤中) 행동(行動))

  • Chun, J.C.;Ma, S.Y.
    • Korean Journal of Environmental Agriculture
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    • v.15 no.4
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    • pp.501-505
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    • 1996
  • Azimsulfuron [1H-pyrazole-5-sulfonamide,N-(((4,6-dimethoxy-pyridine-2-yl-aminocarbonyl-4-(2-methyl-2H-tetrazole-5-yl)] is a new sulfonamide herbicide that selectively controls a wide range of weeds in lowland rice (Oryza sativa). It effectively controlled Cyperus serotinus, Eleocharis kuroguwai, Sagittaria pygmaea, S. trifolia, and Scirpus juncoides at 7.5 - 30 g ai/ha. In the tolerance test on grasses carried out in a nutrient solution containing 0.3 - 30 ppm of azimsulfuron, greater inhibition occurred in roots of both rice and barnyardgrass (Echinochloa crus galli) than in shoots. However, rice root was approximately 5-fold more tolerant than that of barnyardgrass. The downward movements as determined by 50% growth inhibition of S. juncoides were 4-cm in clay loam and 6.5-cm in sandy loam soil with 3-cm/day leaching for 3 days. When incubated at 20 and $30^{\circ}C$, the residual effect in clay loam soil lasted for 30 and 21 days, respectively. In a soil column applied at 15 g ai/ha of azimsulfuron followed by 3-cm/day leaching for 3 days, dry weights of S. trifolia emerging at 5, 10, and 15-cm depth were reduced to 87, 85, and 79% of the corresponding untreated control, respectively. Susceptibility of S. trifolia to azimsulfuron did not greatly vary with the emergence depth.

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The Policy Guideline of Outdoor Advertising Signs Design Study for Street Scened of City (도시의 가로경관 향상을 위한 옥외광고물 디자인 정책 방향 연구)

  • 이중엽
    • Archives of design research
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    • v.11 no.2
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    • pp.71-81
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    • 1998
  • Outdoor advertising signs of sity is recorgnized as the most basic element forming street scenes of city as well as establishing the citys identity in firsthand and secondhand. It has just started by commercial measure but recntly, in the rapid economical growth and diversified social structure a grent number of business have been generated so that is brings an absoluce increage of kind and quantity in outdoor advertising signs. Such quantitative increase has caused now envirenmental city problems so a more effective control of outdoor advertising signs and sophisticatided and logical design is needed. Thus, I would like to set up the direction with street scenes of city can be formed through this study. Firstly, understanding the concept, special features, and strong, weark points of street scenes of city and outdoor advertising signs. Secondly, in present sitiation analysis, studing and comparing the chracteristic of our present outdoor advertising regulations with those of Japanese regulations. Thirdly performing the reserch upon now advertising signs can be influentia in city scenes and thereafter, issuing possible problem censequantly, I would like to suggest the direction of control policy of outdoor advertising signs as following (1). Leading differentiation of each reggion for the solution of environmental and scenic problem. (2). Non-realistic advertising regulation are needed to be amended for uping changing outdoor advertising signs. (3). Advertising problems should be settleed through effectiveness of outdoor advertising signs administration. (4). The problem of advertising agenics should be leaded short term and long term plan. (5). Collection of scenic pollution should be operated for building up a scenic and community consciousness. Therefore, with these suggestion of clear direction of polish, the improvement of city scenes and regional differentiation followed by activated local autonomy system can be induced so that a more beautiful city scenes can be achiveved.

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Biological Probiotic Properties of Lactobacillus rhamonosus GG-4 Isolated from Infant Feces (유아분변으로부터 분리한 Lactobacillus rhamonosus GG-4의 생균제적 특성)

  • Kang, Jin-Hae;Kim, Dae-Hwan;Lee, Sang-Won;Kim, Hong-Chul;Cho, Young-Un;Gal, Sang-Wan
    • Journal of Life Science
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    • v.20 no.12
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    • pp.1882-1888
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    • 2010
  • To develop probiotics, a kind of Lactobacillus sp. was isolated from infant feces. The bacterium was identified as Lactobacillus rhamnosus through 16S rDNA sequence analysis. The strain was a facultative anaerobe which grew better in aerobic conditions. The bacterium lowered the pH of the culture solution down to 2.4 during 48 hr in the MRS medium. The strain inhibited the growth of 6 pathogens - S. aureus, L. monocytogens, S. typhimurium, E. coli O-157, V. parahaemolyticus and P. aeruginosa. When the Lactobacillus were fed to chickens, along with commercial feed, for one month, amounts of $H_2S$ and $NH_3$ in the feces of the chicken decreased to 50% and 70%, respectively, compared to those of control group chickens. Amounts of other bad smells such as $(CH_3)SH$, $(CH_3)_2S$ and $(CH_3)_2S_2$ were not much different in the Lactobacillus-fed chickens compared to the control group. On the other hand, egg weights of the chickens fed Lactobacillus were higher by about $5{\pm}1\;g$ than those in the control group.

Measurement of $\textrm{CO}_2$ Concentration and Leaf Area Index for Crop Photosynthesis Model in Sweet Pepper (단고추의 작물 광합성 모델을 위한 $\textrm{CO}_2$ 농도와 엽면적지 수 측정)

  • Lee, Beom-Seon;Chung, Soon-Ju;Jang, Hong-Gi
    • Journal of Bio-Environment Control
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    • v.8 no.3
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    • pp.192-201
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    • 1999
  • This study was aimed to introduce the measurement of $CO_2$ concentration and leaf area index in the phytotron for predicting the effect of CO.E, light and leaf area index on the instantaneous photosynthetic rate of sweet pepper with the existing ASKAM model. Measurements were made in 2 semi-closed phytotron compartments in which three different $CO_2$ concentrations were applied at random. Plants were grown on containers with circulating nutrient solution at 21$^{\circ}C$ and 80-95% relative humidity. The model estimates crop net $CO_2$ uptake for short time intervals during the day based on short-term data of daily radiation, temperature and $CO_2$ concentration. During the photosynthesis measurements, $CO_2$ concentrations in both compartments and in the basement were measured every minute. This was also done for the flow of pure $CO_2$ into the compartment, global radiation, photosynthetic active radiation inside the compartment, temperature and relative humidity. Crop growth models summarize our knowledge on crop behavior and have as such a wide range of applications in analysis, crop management and thus as a farm management tool.

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