• Title/Summary/Keyword: Soluble nitrogen

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Nitrogen Compounds of Korea ginseng and Their Physiological Significance (고려인삼의 함질소 화합물과 생리학적 중요성)

  • Park, Hoon;Cho, Byung-Goo;Lee, Mee-Kyoung
    • Journal of Ginseng Research
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    • v.14 no.2
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    • pp.317-331
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    • 1990
  • Nitrogen compounds of Panax ginseng and their biological activities in plant and animal were reviewed. Major nitrogen combounds found in P. ginseng are free amino acids, Water soluble teins, insoluble proteins and peptides. Minor nitrogen compounds are dencichine. glycol)roteins. amines, alkaloides, methoxy or alkyl pyrazine derivatives. free nucleosides and nllrleir arid bases. 4-me- thymi-5-thiazoleethanol and pyroglutamic acid. The contents of total nitrogen and protein in root increased until 13 years old rvhich was the highest age tinder investigation. Soluble protein content increased With the root weight and was higher in xylem pith than cortex-epidermis indicating the rlosc relation with root growth. Arginine which covered 58% of total free amino aroids may serve as a storage nitrogen. Arginine seems to be changed into proline in rhizome, threonine in stem and again threoning and arginine in leaf. The greater the root weight the higher the polyaminc content. Polyamine stimulated the growth of root callus. Physiological roles of other minor nitrogen compounds are unknown although dencichine content is relatively high (0.5% d.w.). biochemical and pharmatological activities of some nitrogen compounds for animal were more investigated than physiological roll iota plant itself. Radiation and U.V. protective function (heat stable protein), insulin-like activity in lipogenesis and lipolysis (adenosine and pyroglutamic acid), depression of blood sugar content (glycopeptide). hemostatir and nellrotoxic activity (denrichine) and. sedative and hypnotic activity (4-methyl-5-thiazoleethilnol) are reported. Heat stable protein increased with root age. The traditional quality critsria appear to be well in accordance with biological activities of nitrogen compounds. Chemical stlldies of nitrogen compounds seem relatively rare, probably dole to difficulty of isolation, subsequently the investigations of biological activities are little.

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Performance Estimation of SBR Aerobic Digestion Combined with Ultrasonication by Numerical Experiment (수치실험을 통한 초음파 결합형 SBR 호기성 소화의 거동 예측)

  • Kim, Sunghong;Kim, Donghan;Lee, Dongwoo
    • Journal of Korean Society of Water and Wastewater
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    • v.27 no.6
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    • pp.815-826
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    • 2013
  • Using a developed mathematical model and calibrated kinetic constants, numerical experiments for a aerobic digestion of wastewater sludge by SBR aerobic digestion process combined with ultrasonication (USSBR) were performed in this study. It simulated well the phenomena of the decomposition of particulate organics and the release of organic nitrogen and transformation. To achieve 40 % of particulate organics removal, USSBR process requires only 6 days of SRT and 14 W/L of ultrasonic power whereas SBR aerobic digestion process requires 12 days of SRT. Based on the model simulation results, an empirical equation was presented here. This equation will be used to predict digestion efficiency for the given variables of SRT and ultrasonic power dose. USSBR aerobic digestion process can reduce the nitrogen concentration. The optimal operation strategy for the simultaneous removal of solids and soluble nitrogen in this process is estimated to 7 days of SRT with 14 W/L of ultrasonic power dose while anoxic period was 6 hours out of 24 hours of cycle time. In this condition, 40 % of particulate organics as well as 36 % of total nitrogen will be removed and the soluble nitrogen concentration of the centrate will be lower less then 40 mg/L.

A study on the changes of the components in the steaming and roasting green tea after heat treatments according to time. (가열처리시간이 Steaming 및 Roasting green tea의 성분변화에 미치는 영향)

  • 고영수
    • Journal of the Korean Home Economics Association
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    • v.23 no.2
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    • pp.29-36
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    • 1985
  • This experiment was conducted to investigate changes of the components in steamed and roasted green tea after 10 min, 20 min and 30 min of heat treatment of heat treatment at $110^{\circ}C$. Vitamin C and tannin were determined by spetrophotometry. Total nitrogen, water-soluble nitrogen and water-soluble non protein nitrogen were determined by Kjeldahl analysis system. Aromas were identified by gas chromatography. After heat treatment, contents of most compounds decreased. Tannin and caffeine decreased a little bit, while vitamin C decreased considerably. There were both decreases and increases in aromas. Aromas which increased were geraniol, trans-2-hexenol, linalool, $\alpha$-ionone and $\beta$-ionone. From these results, it was concluded that the highest quality of green tea was one steam- treated for 10 min at $110^{\circ}C$.

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EFFECTS OF UREA NITROGEN ON THE METABOLISM OF PLANTS (1) Studies on Nitrogen Absorption and Metabolism in Sunflower Leavessprayed with Urea Solution

  • KIM, Joon Ho
    • Journal of Plant Biology
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    • v.4 no.2
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    • pp.51-61
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    • 1961
  • In order to detect the way of absorption and metaboism of the urea it is sprayed on the surface of the leaves of sunflower. The sunflowers used in this study are grown in different conditions such that the one in nittogen aboundant and the other in nitrogen deficient soil, respectively. The urea-N, ammonia-N, amide-N, and 80% alcohol soluble-N in the leaves were quantitatively determined. All of the nitrogenous components measured are generally tended to increased with rising the concentration of urea except only amide-N at 24 hours after sprayed, and these were highly significances. It seemed that hydrolizing of urea into ammonia and carbon dixide and the assimilation of ammonia into other organic nitrogenous constituents were rapid in the young leaves than in the mature. It is interest that the amide content, in the young leaves and nitrogen defieient one were enhanced with the increasing concentration of urea, although in the mature leaves it did not show any change in the urea treatment. It is presumed that the assimilation rate of ammonia and the urease activity were lower in the matture leaves than in the young and nitrogen deficient leaves. No significance at 5% level showed all of the nitrogenous components except total nitrogen between nitrogen abundant and deficent leaves. Urea content was a high peak at first 12 hours, ammonia at 48 hours, and amide and alcohol soluble nitrogen at 96 hours, whence decrease4d the content of these constituents gradually. The total nitrogen content is not incrased obviously by only one time of urea spray in this study. When the concentration of urea was relatively high there appeared the wilting spots on t도 edge of leaves. As a whole, it seemed that sprayed urea was rapidly absorbed and taken part in nitrogen metabolism within relatively short period.

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Changes of Nitrogen Compouds and Free Amino Acid of Mung-bean Sprout (녹두나물 성장과정중의 실소화합물과 유리아미노산의 변화에 관한 연구)

  • 김경자
    • Journal of the Korean Home Economics Association
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    • v.19 no.1
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    • pp.25-31
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    • 1981
  • The changes of various nitrogenfractions and free amino acid composition were investigated at various growing stages. The results are summarized as follows 1) Total nitrogen, water soluble protein nitrogen, and true protein nitrogen were decreased with growth. 2) Free amino acid increased with sprout-growth. the content of free amino acid were about 74.4%(combined Ser, Asp, Arg, Val) after 4 day sprout. It is belived that serine, aspartic acid, arginine and valine play an important role as taste compounds in mungbean sprout. 3) 16kinds of amino acid, including essential amino acids in human nutrition except tryptophan and cystin were quantified.

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Changes of Ingredient in Traditional Andong Sikhe using Lactic Acid Bacteria and Yeast (젖산균 및 효모를 이용한 전통 안동식혜의 성분 변화)

  • Kim, Sung;Lee, Son-Ho;Choi, Hee-Jin;Jo, Guk-Young;Choi, Cheong
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1388-1393
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    • 1998
  • Traditional Andong sikhe was produced by fermenting L. bulgaricus LBS 47 and S. cerevisiae SCS 5. The changes of nitrogen compound and amino acid during fermentation and storage were investigated. Crude protein was increased until 4days, the main fermentation period. Amino form nitrogen increased up to 37.50 mg% at the 2nd day of fermentation and the product tasted best at this time. Water soluble and salt soluble protein decreased during fermentation. Proline and aspartic acid were the two major free amino acids. The free methionine increased while the free lysine decreased in the process of fermentation. The amino acids of water soluble protein and salt soluble protin were totally 17 kinds. The major amino acids of water soluble and salt soluble protein were glutamic acid and aspartic acid. The arginine content of salt soluble protein increased as the fermentation proceeded.

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Effects of Commercial Food Grade Enzyme on Acceleration of Ripening in U.F. Cheese Base Slurries (효소 첨가가 U.F. 치즈베이스 slurries의 숙성촉진에 미치는 영향)

  • Yoon, Kyung;Kwak, Hae-Soo
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.510-516
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    • 1993
  • This study was conducted to investigate biochemical characteristics of enzyme-added cheese base slurries during accelerated ripening. Trichloroacetic acid (TCA) soluble nitrogen of cheese base slurries increased rapidly during the first day of ripening and the rate of increase slowed down thereafter. Cheese base slurries showed lower level in the production of the nitrogen than Cheddar cheese slurries. Producctions of phosphotungstic (PTA) soluble amino nitrogen also showed similar trends as TCA soluble nitrogen. Electrophoresis revealed that all caseins in both cheese base slurries and Cheddar cheese slurries were hydrolyzed, but whey proteins in cheese base slurries were little hydrolyzed. Cheese base slurries produced free amino acids little more than half of Cheddar cheese slurries. Both slurries showed similar increasing trend in production of short-chain free fatty acids. The specificity of the fatty acids in the slurries was similar to that of natural ripened cheese. The results of this study showed that addition of enzyme was effective to accelerate cheese base ripening.

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Studies on the Physiological Chemistry of Germination in Ginseng Seed (인삼종자발아에 있어서의 생리화학적 연구)

  • Jong-Kyu Hwang;Hee-Chun Yang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.17
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    • pp.135-142
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    • 1974
  • A study on the metabolism of the chemical companents of endosperm and enbryonic othans of ginseng seeds during their germination were inverstigated and the results of the changes in the contents of nitrogen conponds, carbohydrates, lipids and phosphorus conpounds are summarized as follow; 1. When a seeding grows to 5cm the fresh weight of the embryonic organ incerases 13 times compared with that of its ripened embryo veore germination and its dry weight increases 4.5 times. On the other hand, about 65% of the dry weight of the endosperm is lost. 2. During germinarion the total nitrogen content of a sedding (endosperm+embryonic organ) decreases and when the seeding grows to 5cm there is a loss of 10% of total nitrogen content. At this time, soluble nitrogen content amounts to 40~50% of the total nitrogen, a comparatively high content. 3. When theseeding grows to 5cm, the total phosphorus content decreases by 15%. During the germination period 70~75% of the total phosphorus is distributed in the embryonic orang and 25% of it is in endosperm.In the embryonic organ 35~50% of the acid soluble phosphorus is inorganic phosphorus and in the endisperm, 20~25% of the acid soluble phosphorus is inorganic phosphorus,75~80% of the organic phosphorus is contained in the endosperm. 4.One the seedling grew to 2~3cm, carbohydrates such as soluble sugars,reducong sugars,nonreducong sugars, and crude starch interconverted remarkably. 5.After stratification (just before germination) the lipid content of the endosperm is about 54% of the total weight and lipid content of the embryo is about 61%. During germination 6.81mg of the fat contained in the endosperm per seed decreases to 4.13mg while the change of fat content in the embryonic organ is not so great.

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Effect of Strawberry Puree on the Physicochemical Properties of Kochujang

  • Kim, Hui-Jeong;Seog, Eun-Ju;Lee, Jun-Ho
    • Preventive Nutrition and Food Science
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    • v.12 no.3
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    • pp.185-189
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    • 2007
  • Quality characteristics of kochujang prepared with strawberry puree (10, 20, and 30% on the total weight basis) were investigated after 30 and 300 days of storage. The moisture content of strawberry kochujang at 300 days was considerably higher than that of strawberry kochujang at 30 days and increasing strawberry content from 0 to 30% significantly increased moisture content of kochujang progressively (p<0.05). Water activity and titratable acidity also showed similar trends, but pH showed the reverse trend with the highest value in control at 30 days and the lowest value in 30% strawberry kochujang at 300 days. As the strawberry puree content increased, amino-nitrogen content consistently and correspondingly decreased. Amino-nitrogen content at 300 days was higher than that of strawberry kochujang at 30 days. Soluble solids content also showed a similar trend, but soluble solid content at 300 days was considerably lower than that of strawberry kochujang at 30 days. Reduction in soluble solids content with increasing strawberry concentration was more evident at 30 days than at 300 day.

Some Factors Affecting Glucoamylase Production from Aspergillus sp. (Aspergillus sp.의 Glucoamylase 생산에 미치는 요인)

  • Park, Inshik;Youngho Chung
    • Microbiology and Biotechnology Letters
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    • v.17 no.5
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    • pp.519-523
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    • 1989
  • The effects of carbon, nitrogen sources and culture conditions on glucoamylase production from Aspergillus sp. were investigated. Among tested carbon sources, soluble starch was most effective for the production of the enzyme, and the level of concentration for the optimal enzyme production was found to be 5%. For nitrogen sources, yeast extract was best for the enzyme production, with the level of 0.1%. The enzyme was maximally produced by cultivating the organism at medium of initial pH 6.0, and temperature of 28$^{\circ}C$. Wheat bran was most suitable for the enzyme production from the organism in solid state culture.

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