• Journal of the Korean Wood Science and Technology
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• v.33 no.5 s.133
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• pp.92-98
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• 2005

This study was performed to get the basic information concerned to the optimum culture condition of Inonotus obliquus. Several solid media, PDA, MEA and Czapek-Dox, and three liquid media were adopted for the in vitro cultivation. Some main features of the fungal morphological characteristics under cultivation conditions were observed and described. Preliminary results showed that appearance of the mycelial mat, hyphal size and substrate pigmentation differed according to the media. The PDA medium was the most favorable substrate for the growth on solid culture, followed by MEA and Czapek-Dox media. Concerned to the addition of amino acids, 5 amino acids, such as alanine, alginine, isoleucine, leucine and threonine, enhanced to the mycelial growth. Isoleucine was shown the best fungal growth. An important morphological hyphal structure for the fungus, the setae, was found in abundance and diverse its shape and size. In liquid culture, fresh potato broth was the best growth stimulant of the fungus, followed by Malt extract and potato broth. Addition of yeast extract to the liquid media had improved the biomass, but not laccase production.

• The Journal of the Korean dental association
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• v.11 no.8
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• pp.525-530
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• 1973

True collagenolytic enzymes in animal tissues were first demonstrated by Gross and Lapiere (1962), who showed the ability of such an enzyme in the culture medium of living explants of tadpole tissue to degrade a specific substrate of undenatured collagen under physiological conditions. Recently, tumor-associated collagenolytic activity has been demonstrated in human neoplasm and in ascites V Carcinoma. This investigation have been peforme to determine whether or not a collagen lytic enzyme could e found in isolated solid Tawa sarcoma of Donryu female rat obtained the culture medium. The results were as follows. 1. 11.5mg% of hydroxyproline contained in Donryu rat skin collagen, which was extracted by 0.5M acetic acid. 2. Cultivation of solid Tawa sarcoma tissues on reconstituted rat skin collagen gels showed lysis of adjacent gel after 18 hours, and much more extensive lysis after 5 days. 3. Collagen substrate was not attacked by the common proteolytic enzymes, trypsin, pepsin, and pronase.

• Journal of Mushroom
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• v.22 no.1
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• pp.8-16
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• 2024

Paecilomyces tenuipes (P. tenuipes) is a fungus cultivated artificially by South Korean researchers, utilizing rice bran as its substrate. The increased demand for this fungus has not been met with successful cultivation methods for fruiting body production in natural environments. Therefore, we tested the effect on the growth of P. tenuipes using a Solid media based on pests. In this results, the Solid media based on M.alternatus was effective in increasing the growth of P. tenuipes and the content of cordycepin. Moreover, we confirmed the conditions for manufacturing a Solid media based on M.alternatus for P. tenuipes growth. We suggested that the growth-promoting compounds offers valuable insights for optimizing fungal cultivation conditions, thereby enhancing productivity and contributing to a broader understanding of fungal physiology in varying nutritional environments.

• Mycobiology
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• v.42 no.4
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• pp.346-352
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• 2014

Recently, the Q biotype of tobacco whitefly has been recognized as the most hazardous strain of Bemisia tabaci worldwide, because of its increased resistance to some insecticide groups. As an alternative control agent, we selected an Isaria javanica isolate as a candidate for the development of a mycopesticide against the Q biotype of sweet potato whitefly. To select optimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between 2 substrates (barley and brown rice), and we also compared the effects of various additives on conidia production and virulence. Barley was a better substrate for conidia production, producing $3.43{\times}10^{10}$ conidia/g, compared with $3.05{\times}10^{10}$ conidia/g for brown rice. The addition of 2% $CaCO_3+2%$ $CaSO_4$ to barley significantly increased conidia production. Addition of yeast extract, casein, or gluten also improved conidia production on barley. Gluten addition (3% and 1.32%) to brown rice improved conidia production by 14 and 6 times, respectively, relative to brown rice without additives. Conidia cultivated on barley produced a mortality rate of 62% in the sweet potato whitefly after 4-day treatment, compared with 53% for conidia cultivated on brown rice. The amendment of solid substrate cultivation with additives changed the virulence of the conidia produced; the median lethal time ($LT_{50}$) was shorter for conidia produced on barley and brown rice with added yeast extract (1.32% and 3%, respectively), $KNO_3$ (0.6% and 1%), or gluten (1.32% and 3%) compared with conidia produced on substrates without additives.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.525-531
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• 2010

This work was aimed at utilizing rice bran as a substrate for $\beta$-carotene production by Rhodotorula glutinis DM 28 under optimized conditions of solid-state fermentation. The biomass and $\beta$-carotene content of Rhodotorula glutinis DM 28 grown on rice bran as a sole substrate under solid-state fermentation were 54 g/kg rice bran and 1.65 mg/kg rice bran, respectively. Its biomass and $\beta$-carotene content, however, could be improved by 60% and 30%, respectively, using the Central Composite Design for the optimization of its cultivation conditions. The optimized conditions obtained were a pH of 5, a moisture content of 70% (w/w), and a carbon-to-nitrogen ratio of 4. Under these conditions, rice bran containing R. glutinis DM 28 had nutritional values of $\beta$-carotene, protein, and fat higher than those of rice bran alone. Yeast-grown rice bran could be suitable, therefore, to use as a $\beta$-carotene-enriched supplement in animal feeds.

• Journal of Environmental Science International
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• v.33 no.1
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• pp.17-25
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• 2024

This study aimed to determine the most suitable coir substrate mixing ratio for optimizing the growth and yield of the "lpduelkkae 1" cultivar. We comprehensively analyzed the physicochemical properties, growth, and yields of four different substrate combinations: perlite (coir with mixing ratios of 70:30 (PC30), 50:50 (PC50), and 30:70 (PC70)) and 100% coir (C100). The results revealed substantial differences in substrate properties. C100 exhibited the highest total porosity and the lowest solid phase, indicating excellent air permeability. The pH levels and electrical conductivity (EC) values ranged from 5.4-6.8 and 1.2-3.1 dS·m^-1, respectively. Leaf growth parameters, including length, width, and dry weight, showed positive correlations with high coir ratios, except for PC30. PC70 and C100 outperformed other substrates in stem growth, exhibiting superior stem diameter and fresh and dry weights. The quantity of marketable leaves was the highest in the C100 substrate. Furthermore, C100 comprised integrated levels of essential nutrients, such as Ca and Mg, owing to its high coir content. In conclusion, a coir ratio of approximately 70% (v/v) should be maintained in the substrate for creating an optimal cultivation environment. Furthermore, the selection of humidity-resistant varieties as well as precise nutrient and moisture management for different seasons and growth stages are crucial for a successful perilla leaf hydroponic cultivation.

• Journal of Environmental Science International
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• v.28 no.9
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• pp.729-735
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• 2019

The aim of this study was to determine the effects of different compositions of environmental substrates on hydroponic tomato cultivation. Three different substrates were used in coir chip:dust (v/v=50:50; CP1), coir chip:dust (v/v=80:20; CP2), and rock wool cube with CP2 (CPR). The amount of irrigation during the cultivation period was 190 mL/(plant time) in all substrates. The pH and EC were 5.8-6.2 and 2.6-2.9 dS/m, respectively. The drainage rate in CP1 was 31%, in CP2 was 36%, and in CPR was 29%. The growth of tomato plants in terms of height was higher in CP1 and CPR. The leaf area was greater in CP2. The fresh and dry weights were greater in CP2 and CPR treatments. The net photosynthesis in CP2 ($19.31{\mu}mol\;CO_2/m^2s$) and root activity in CP2 were higher among all three treatments. The soluble solid content of fruit was not significantly different among treatments. The yield per plant in CP2 and CPR treatments was 17% greater than the yield per plant in CP1. Therefore, the most suitable substrate for hydroponic tomato cultivation is the substrate mixed with coir chip:dust (v:v=80:20; CP2), i.e., CPR.

• Microbiology and Biotechnology Letters
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• v.31 no.3
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• pp.257-263
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• 2003

The cellulases production in solid state fermentation (SSF) of Trichoderma harzianum FJ1 with high cellulases productivity using cellulosic wastes was investigated. Physical and chemical conditions of the fermentation, such as moisture content, initial pH, and composition of mixed substrate (wine waste, rice straw, and soybean flour) on FPase (Filter paper activity) production were examined. The enzyme production was optimized in the conditions of moisture content of 70%, pH 5.0, 3$0^{\circ}C$, and 1:1:1 composition of mixed substrate containing wine waste, rice straw, and soybean flour. The highest activities of FPA, CMCase, Xylanase, $\beta$-glucosidase, and Avicelase in the optimized culture conditions were 15.2, 69.1, 83.9, 29.2, and 4.2 unit/g-SDW in 5 day cultivation, respectively. Economical and efficient production of cellulolytic enzymes by T harzianum FJ1 using cellulosic wastes in solid state fermentation will contribute to the biological saccharification of cellulosic wastes with enormous potential resource value in future.

• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.29-29
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• 2018

Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

• Journal of Applied Tourism Food and Beverage Management and Research
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• v.13 no.1
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• pp.69-80
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• 2002

The grains were used as solid-substrate for the cultivation of basidiomycetes. The grains were fermented with Ganodema lucidum 7094, Fomitella fraxinea 81003, Phellinus igniarius 26005, and its free sugar composition was investigated. For the mass cultivation of mycelia, the hydrated grains with cold water were put into the plastic bottle. The mycelial growth rate in the bottled grains was high in the early stage with inoculation of homogenized mycelium. The activity of mycelium was maintained by adding sterilized water in the middle of cultivation. There was marked difference in the content of total free sugar and composition ratio of free sugar according to kinds of basidiomycetes. The content of free sugar increased far more in grains fermented with mycelium than in grains which was not fermented.