• 제목/요약/키워드: Solid culture

검색결과 602건 처리시간 0.024초

더덕 속 식물의 부정근 유도와 증식 (Induction and Proliferation of Adventitious Roots in Codonopsis spp.)

  • 김지아;박응준;최용의
    • 한국약용작물학회지
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    • 제20권6호
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    • pp.493-499
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    • 2012
  • We have established adventitious root culture systems of Codonopsis lanceolata, Codonopsis pilosula and Codonopsis ussuriensis. Root segments of C. lanceolata were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $0.5mg/{\ell}$ NAA and produced $18.8{\pm}1.9$ roots per explant. Root segments of C. pilosula were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $1.0mg/{\ell}$ NAA and produced $8.5{\pm}1.8$ roots per explant. Leaf segments of C. ussuriensis were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $0.5mg/{\ell}$ NAA and produced $7.8{\pm}0.4$ roots per explant. In liquid culture, the best production of adventitious root (fresh weight) was obtained in 1/2 MS medium with $1.0mg/{\ell}$ NAA. This study demonstrated for the first time to produce adventitious roots in C. pilosula and C. ussuriensis.

Glucoamylase Production in Batch and Fed-Batch Solid State Fermentation: Effect of Maltose or Starch Addition

  • Bertolin, Telma Elita;Jorge Alberto Vieira Costa;Gean Delise Leal Pasquali
    • Journal of Microbiology and Biotechnology
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    • 제11권1호
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    • pp.13-16
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    • 2001
  • Maltose and soluble starch were used as secondary sources of carbon for glucoamylase production by Aspergillus awamori in solid state fermentation. During batch cultivation, maltose above 2.5%(w/w) repressed glucoamylase production, but, by adding either 2.5% (w/w) maltose or 1.25% (w/w) soluble starch to fed-batch cultivations, glucoamylase activity was increased by 15% and 170% over standard medium, respectively. The data showed that maltose is a weak inducer of glucoamylase production in solid stat fermentation.

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Overexpression of Shinorhizobium meliloti Hemoprotein in Streptomyces lividans to Enhance Secondary Metabolite Production

  • Kim, Yoon-Jung;Sa, Soon-Ok;Chang, Yong-Keun;Hong, Soon-Kwang;Hong, Young-Soo
    • Journal of Microbiology and Biotechnology
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    • 제17권12호
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    • pp.2066-2070
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    • 2007
  • It was found that Shinorhizobium meliloti hemoprotein (SM) was more effective than Vitreoscilla hemoglobin (Vhb) in promoting secondary metabolites production when overexpressed in Streptomyces lividans TK24. The transformant with sm (sm-transformant) produced 2.7-times and 3-times larger amounts of actinorhodin than the vhb-transformant in solid culture and flask culture, respectively. In both solid and flask cultures, a larger amount of undecylprodigiocin was produced by the sm-transformant. It is considered that the overexpression of SM especially has activated the pentose phosphate pathway through oxidative stress, as evidenced by an increased NADPH production observed, and that it has promoted secondary metabolites biosynthesis.

The Introduction of Polycrylamide Gel into the Solid Culture of Streptomyces spp

  • Han, Hong-Ui;Yang, Moon
    • 미생물학회지
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    • 제30권1호
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    • pp.65-69
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    • 1992
  • It is proposed the polyacrylamide gel, instead of agar, could be used for the solid cultures of microorganisms including Streptomyces strains. Polymerization and gellation of 5% acrylamide solution were done by autoclaving for 5 min at 121.deg.C and no hindered by the addition of nutrient-rich media. In particular, pH buffer solution suitable for corresponding microorganisms must be used in the preparation of culture media. Comparing with agar, it was discussed that polycrylamide gel had many advantages such as gellation within the wide range of strong acid Carbon and Nitrogen sources, requirement tests of growth factors and minerals, sterization at high temperature, diffusion assays of products depending on the pore size of gel, and stability and standarization of microbial cultures.

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위탁 경영사업체 및 대학교 급식소의 수행평가 (The Performance Measurement of Business & Industry and University Foodservice Operated by Contracted Foodservice Management Company)

  • 한경수
    • 한국식생활문화학회지
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    • 제17권3호
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    • pp.252-259
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    • 2002
  • The purpose of this study examined important factor of performance on Contract Foodservice management. Three contract Foodservice companies were selected which were located in Seoul & kuonggi province. 300 units were chose. The questionnaire was composed of seven part. There were the operation of food management, the operation of menu, the operation of equipment and facilities management, the operation of solid waste and safety management, the operation of waiting time management. As a result of the survey B&I foodservice was listed higher than University foodservice as food hygiene, FIFO and food shelf life. University foodservice employees worked 4 hour longer than B&I foodservice employees, so turnover rate was higher than it. Comparing the number of feeding, B&I foodservice equipment and facilities scale was bigger than University foodservice equipment and facilities. The rate of solid waste on B&I foodservice was 11.468% and the rate of University foodservice was 16.23%, the waiting time of University foodservice was about 8mins longer than B&I foodservice.

Increasing Trend of Isolation of Non-Tuberculous Mycobacteria in a Tertiary University Hospital in South Korea

  • Yoo, Jung-Wan;Jo, Kyung-Wook;Kim, Mi-Na;Lee, Sang-Do;Kim, Woo-Sung;Kim, Dong-Soon;Shim, Tae-Sun
    • Tuberculosis and Respiratory Diseases
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    • 제72권5호
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    • pp.409-415
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    • 2012
  • Background: The isolation of non-tuberculous mycobacteria (NTM) has been increasing in South Korea. To date, however, the cause of this increase has not been determined, and it remains unclear whether the use of liquid media has contributed to this increase. The aim of this study was to evaluate the factors associated with NTM isolation and the impact of liquid media on NTM culture. Methods: Mycobacterial smear/culture results of respiratory specimens (sputum and bronchial aspirates), obtained during the years 2002, 2005, and 2010, were retrieved and analyzed retrospectively. Results: During the years 2002, 2005, and 2010, 83,096 sputum specimens were collected from 31,104 patients, and were cultured for mycobacteria, using solid media only in the 2002 and 2005 specimens and both solid and liquid media in the 2010. Of these, 3,516 (4.2%) specimens were smear-positive for acid-fast bacilli (AFB). The annual rate of NTM among positive culture specimens increased from 21% in 2002 to 57.8% in 2010 (p<0.001), as did the proportion of NTM, among AFB smear- and culture-positive specimens, from 12.2% in 2002 to 45.2% in 2010 (p<0.001). In 2010, the NTM culture rate was higher in the liquid than in the solid media (13.9% vs. 8.4%, p<0.001). The NTM rate among AFB-positive specimens was higher in patients aged >50 than ${\leq}$50 years. Conclusion: The rate of NTM isolation has steadily been increasing at the hospital in South Korea, likely due in part to the use of liquid media for the culture.

Xylanase Production by Mixed Culture Using Crude Hemicellulose from Rice Straw Black Liquor and Peat Moss as an Inert Support

  • Shata, Hoda Mohamed Abdel Halim;El-Deen, Azza Mohmed Noor;Nawwar, Galal Abdel Moen;Farid, Mohmed Abdel Fattah
    • Journal of Applied Biological Chemistry
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    • 제57권4호
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    • pp.313-320
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    • 2014
  • Black liquor (BL) is a by-product of rice straw pulping process. It is a low costs raw material for production value-adding proteins and enzymes, which has been paid more and more attention to reduce its environmental pollution. Mixed cultures of micelial fungi, Trichoderma reesei Northern Regional Research Laboratory (NRRL)11236, Trichoderma reesei NRRL 6165 and Aspergillus niger strains NRC 5A, NRC 7A, and NRC 9A were evaluated for their ability to produce xylanase using crude hemicellulose (CHC) prepared from BL and peat moss as an inert support under solid state fermentation (SSF). The most potent strains, A. niger NRC 9A (818.26 U/g CHC) and T. reesei NRRL 6165 ($100.9{\pm}57.14$ U/g CHC), were used in a mixed culture to enhance xylanase production by co-culturing under SSF. In the mixed culture, xylanase production ($1070.52{\pm}12.57$ U/g CHC) was nearly1.3 and 10.6-fold increases over the activities attained in their monocultures, A. niger NRC 9A and T. reesei NRRL 6165, respectively. Optimization of the culture parameters of the mixed culture SSF process, concentration of ammonium sulfate and corn steep liquor, CHC/peat moss ratio, inoculum size and ratios of the two strains, initial pH value, initial moisture content and incubation time, exhibited a significant increase ($2414.98{\pm}84.02$ U/g CHC) in xylanase production than before optimization.

생물반응기에서 현삼의 신초 형성과 발근 (Production and Rooting of Shoots in Bioreactor Culture of Scrophularia buergeriana Miquel)

  • 한석훈;채영암
    • 한국약용작물학회지
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    • 제8권2호
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    • pp.117-122
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    • 2000
  • 본 실험은 생물반응기를 이용한 현삼 종묘의 기내생산과 기내 발근에 관여하는 요인을 검토하여 추후 종묘의 건전한 생육과 안정적 생산에 활용할 수 있는 기초자료를 얻고자 실험한 결과는 다음과 같다. 1. 생물반응기에서 현삼 배양은 MS 기본배지의 $NH_4NO$ 양을 1/4 로 줄여 413mg/ l 의 농도로 처리하고 6주간 배양하는 것이 신초의 유도에 유리하였다. 2. 생물반응기 배양시 신초의 형성과 생장에는 $NH_4NO_3$를 413mg/ l로줄인 MS 배지가 유리하였다. 3. 배지량 1.5L 의 생물반응기에서 줄기 절편의 접종량이 300개 이상일 경우 기내 식물체가 과밀화 되어 신초의 생육이 저해되고, 생물반응기 내부 및 배지 위로 노출된 신초는 괴사되었다. 따라서 신초의 생체중이 무거우면서도 신초수를 많게 하기 위해서는 적정 접종량을 300개체로 하는것이 유리하였다. 4. 신초의 발근 수와 길이생장에는 IBA 0.05mg/ l 가 효과적이었다. 5. 생물반응기에서 6주간 배양하는 것이 신초의 생육이 정상적으로 되었으며 또한 이들 신초에서 발근과 뿌리의 발육에도 유리하였다.

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Effect of Abiotic Factors on Fumosorinone Production from Cordyceps fumosorosea via Solid-State Fermentation

  • Tahir Khan;Dong-Hai Hou;Jin-Na Zhou;Yin-Long Yang;Hong Yu
    • Mycobiology
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    • 제51권3호
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    • pp.157-163
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    • 2023
  • Cordyceps fumosorosea is an important species in the genus of Cordyceps, containing a variety of bioactive compounds, including fumosorinone (FU). This study was a ground-breaking assessment of FU levels in liquid and solid cultures. The present study focused on the impacts of solid-state fermentation (SSF) using solid substrates (wheat, oat, and rice), as well as the effects of fermentation parameters (pH, temperature, and incubation period), on the generation of FU. All the fermentation parameters had significant effects on the synthesis of FU. In a study of 25 ℃, 5.5 pH, and 21 days of incubation period combinations calculated -to give maximal FU production, it was found that the optimal values were 25 ℃, 5.5 pH, and 21 days, respectively. In a solid substrate medium culture, FU could be produced from SSF. At 30 days, a medium composed of rice yielded the most FU (798.50 mg/L), followed by a medium composed of wheat and oats (640.50 and 450.50 mg/L), respectively. An efficient method for increasing FU production on a large scale could be found in this approach. The results of this study might have multiple applications in different industrial fermentation processes.

작약의 화분배양에 의한 캘러스 및 배발생 (Callus Induction and Embryogenesis Through Pollen Culture in Paeonia albiflora PALL)

  • 김영숙;이병기
    • 식물조직배양학회지
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    • 제22권1호
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    • pp.13-17
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    • 1995
  • 작약의 화분배양에 의한 반수체 식물을 유기시킬 목적으로 화분배양을 실시하여 배양중 소포자의 변화를 관찰하고 캘러스 및 배발생에 미치는 저온전처리(5$^{\circ}C$/10 days)의 효과와 배양화분의 생존율을 조사하였다. 작약의 화분배양시 캘러스 및 배발생에는 저온전처리의 효과가 다소 인정되었으나 약배양의 효과에는 미치지 못하였다. 화분를 첨가한 고체배지에 이식했을 때 구형배가 형성되었다. 화분의 생존율에는 저온전처리 및 배지조성간 의 영향이 미치지 않았으며 배양기간이 지남에 따라 화분의 생존율도 저하되었다.

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