• Title/Summary/Keyword: Solanum tuberosum

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Identification of Potato mop-top virus from Solanum tuberosum cv. Gawon in Korea

  • Lee, Young-Gyu;Park, Jong-A;Yoon, Young-Nam;Cheon, Jeong-Uk;Lee, Key-Woon
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.138.1-138
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    • 2003
  • Potato mop-top virus(PMTV) was identified from Solanum tuberosum cv. Gawon showing bright chlorotic mottle symptom in Namwon, Korea. Samples were collected green-house in February, 2003. Electron microscopic examination of negatively stained preparation revealed that PMTV were rigid-rod shaped particles about 100-150, 250-300 nm x 18-20 nm in length. In ultrathin sections of leaf tissue from diseased potato plants, cluster of viruses particles were observed in the cytoplasm. TAS-ELISA determined that the virus was serologically related to PMTV. PMTV produced double ring necrotic local lesion in inoculated leaf of Chenopodium amaranticolor in incubated at 15$^{\circ}C$. The PMTV could be detected with RT-PCR using PMTV detectable primer set designed to amplify about 540 bp of the partial CP gene of PMTV

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PVY Resistant Transgenic Potato Plants (cv Claustar) Expressing the Viral Coat Protein

  • Gargouri-Bouzid Radhia;Jaoua Leila;Mansour Riadh Ben;Hathat Yemna;Ayadi Malika;Ellouz Radhouane
    • Journal of Plant Biotechnology
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    • v.7 no.3
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    • pp.143-148
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    • 2005
  • The coat protein mediated resistance to potato virus Y is assessed here in transgenic potato plants (Solanum tuberosum L., cv Claustar). Therefore, the corresponding cDNA from tunisian isolate of the virus was cloned into Agrobacterium tumefaciens binary vector. The transgenic lines were subsequently analysed for the presence and expression of the transgene. The CP cDNA copy number was determined for kanamycin resistant plants. Three selected transgenic lines and their S1 progeny resulting from tuber germination showed a high protection level against the virus. These data appear to support the hypothesis that the virus resistance is mediated by the translated viral coat protein expressed in transgenic potato lines.

Thermostability of Polyphenol Oxidase from Potato (Solanum tuberosum L.) (감자 Polyphenol Oxidase의 열안정성)

  • 김나영;이민경;박인식;방극승;김석환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.844-847
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    • 2001
  • Factors affecting thermostability of polyphenol oxidase (PPO) from potato were studied for the purpose of providing useful information for food processing operations. The enzyme was most stable at pH 7.0 and it was inhibited to 70% after heat treatment at 8$0^{\circ}C$ for 1 min. The z-value for the thermal inactivation of the PPO was 12.17$\pm$0.58$^{\circ}C$. The thermostability of the enzyme was reduced by addition of sodium chloride. And the activity was inhibited by addition of reducing reagents such as 2-mercaptoethanol and dithiothreitol.

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Suppressive Effects of Potato (Solanum tuberlosum) on Type II Collagen-Induced Arthritis in DBA/1J Mice

  • Choi, Eun-Mi
    • Food Science and Biotechnology
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    • v.16 no.1
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    • pp.43-48
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    • 2007
  • Collagen-induced arthritis (CIA) is a model for some types of human autoimmune rheumatoid arthritis (RA). In this study, we examined whether ethanol extract of potato (Solanum tuberosum) is efficacious against CIA in mice. Potato extracts (100 and 200 mg/kg) were orally administered to DBA/1J mice once daily for 49 day after initial immunization with type II collagen. Clinical assessment of disease and measurement of paw edema were conducted throughout the study. The production of CIA-related rheumatoid factor, anti-type II collagen antibody, and cytokines were examined in DBA/1J mice. Serum levels of AST, ALT, creatinine, and lipids were measured, and antioxidant enzyme activity in the spleen was also determined. The arthritis score and paw edema were markedly suppressed in the groups treated with potato extract. Levels of rheumatoid factor, anti-type II collagen antibody, interleukin (IL)-1, IL-6, LDL-cholesterol, and malondialdehyde in sera were also reduced by potato extract treatment. The activities of glutathione peroxidase and glutathione reductase were increased in the spleens of CIA mice treated with potato extract. These findings suggest that potato extract has suppressive effects on type II collagen-induced arthritis, an animal model for human RA.

Anti-hypertensive Activity of New Potato(Solanum tuberosum) Variety of Gui Valley Via Inhibition of Angiotensin Converting Enzyme (안지오텐신 전환효소활성 저해에 의해 항고혈압 효과를 갖는 신품종 감자 구이벨리 추출물)

  • Kim, Kwan-Hyun;Kim, Sun-Hee;Lee, Eun-Ok;Kwon, Hyun-Jung;Choi, Jong-Won;Lim, Hak-Tae;Kim, Sung-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.1
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    • pp.93-96
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    • 2009
  • This study was attempted to investigate the anti-hypertensive activity of Solanum tuberosum, cv. Gui valley. Ethanol extract of Gui vally (EGV) increased free radical scarvenge activity up to 91.6% of control at $200{\mu}g/m{\ell}$. It's anti-oxidant activity is similar to 10 uM of ascorbic acid, well known as antioxidant. EGV inhibited Angiotensin-I-converting enzyme (ACE) activity in vitro. ACE plays a important role in regulation of blood pressure and ACE inhibitors are important for the treatment of hypertension. Anti-hypertensive activity of EGV in vivo was assessed in lead acetate (LAT)-induced hypertensive rats for 8 weeks. Elevated blood pressure in control group was significantly decreased by EGV at 200 mg/kg. Also, ACE activity in blood was also suppressed by EGV treatment. Taken together, these results suggest that EGV has an anti-hypersive activity via inhibition of ACE and can be used for the treatment or prevention of hypertension.