• Title/Summary/Keyword: Soil sterilization

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Influence of Sterilization Methods on Atrazine Assimilation (멸균법이 atrazine의 분해과정에 미치는 영향)

  • 정규혁;오승민
    • Journal of Environmental Health Sciences
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    • v.23 no.1
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    • pp.1-7
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    • 1997
  • We investigated the influence of various known sterilization methods on atrazine assimilation. The present study was designed to investigate the effect of autoclaving, sodium azide and mercuric chloride treatment on the assimilation of atrazine in soil and sediment. The sterilization reactor treated with sodium azide resulted in $^{14}CO_2$ generation and atrazine was rapidly disappeared from reactor through chemical reaction with sodium azide. These findings seem to indicate that sodium azide sterilization is not recommended for atrazine studies. In sample reactors autoclaved or treated with mercuric chloride, $^{14}CO_2$ generation was not detected and most of the disappeared atrazine was found to exist as hydroxyatrazine. These results suggested that autoclaving or mercuric chloride treatment could be effective sterilization methods. However, chemical properties(pH and redox potential) of soil and sediment were altered by any of the sterilization methods applied. So it was suspected that these altered properties could affect distribution and mineralization of atrazine in soil and sediment. In addition, both autoclaving and mercuric chloride treatment have altered $K_d$ values of hydroxyatrazine more significantly than those of atrazine. Consequently, although autoclaving and mercuric chloride treatment are effective sterilization methods, one must be careful in using them in practice as these methods may cause chemical degradation of both of atrazine and its metabolites and changes in chemical properties of soil and sediment. In conclusion, careful assessment of sterilization methods must be made for the degradation studies of chemicals in soil and sediment in order to minimize possible undesirable chemical degradation of sample and/or changes in physico-chemical properties of soil and sediment by the selected sterilization methods.

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Development of Control Method for Strawberry Bacterial Angular Spot Disease (Xanthomonas fragariae) (딸기 세균모무늬병(Xanthomonas fragariae)의 방제를 위한 약제 선발)

  • Kim, Da-Ran;Gang, Geun-Hye;Cho, Hyun ji;Myung, Inn-Shik;Yoon, Hae-Suk;Kwak, Youn-Sig
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.287-294
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    • 2015
  • In Korea, Strawberry bacterial angular leaf spot disease was first reported in 2010. The disease of strawberry caused serious problem for strawberry producers and export. To field test, we applied the bactericides in June and October as nursery stage and cultivation stage, respectively. In nursery stage with high temperature condition, Oxolinic acid (97.2%) showed significant control effect. In cultivation stage with low temperature condition is soil sterilization and soil non- sterilization the control effect. In cultivation stage in soil sterilization stage Validamycin (93.3%) showed reliable control effect against the disease. Also, soil non-sterilization stage with low control effect of Validamycin (80%) showed control effect the lower than a soil sterilization stage.

Underground Heat Transfer Characteristics of the Underground Heating System for Soil Sterilization in Greenhouse (온실 내 토양소독을 위한 지중난방시스템의 지중 열전달 특성)

  • Park, Kyung-Kyoo;Ha, Yu-Shin;Hong, Dong-Hyuck;Jang, Seung-Ho;Kim, Jin-Hyun
    • Journal of Biosystems Engineering
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    • v.35 no.2
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    • pp.108-115
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    • 2010
  • This study was conducted to estimate the optimum temperature and required time for soil sterilization when heated water was circulated through underground heating pipes in the greenhouse which solar heat was influenced to the temperature of soil during the summer day. Two different types of heating pipes were used for the experiment. One was a polyethylene pipe(XL) and the other was a corrugated ring shaped stainless steel pipe(STS). The results of the studies were summarized as follows; By measuring the thermal characteristics of the XL and STS, it was examined that the average temperature differences of the inlet and outlet were $8.5^{\circ}C$ and $13.3^{\circ}C$, the average flowrates were 15.3 L/min and 5.6 L/min, and the average radiation powers were 9.1 kW and 4.1 kW, respectively. As results of the regression analysis of underground temperatures, when average soil temperature was$35^{\circ}C$, an average water temperature was $80^{\circ}C$, and XL was used, it was estimated that the possible heat transfer distance, the required time for heat transfer and heat flux to reach the underground temperature of $60^{\circ}C$ were 300 mm, 230 hours, and $7.57kW/m^2$, respectively.

Improved Cleaning Method for Dental Instruments

  • Kim, In-Geol;Lee, Yun-Ji
    • Journal of Korean Dental Science
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    • v.3 no.2
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    • pp.26-33
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    • 2010
  • We searched at the "PubMed.gov" and "jendodon.com" sites to conduct a literature review on dental instruments that are reused in clinical settings and on infection control involving pre-disinfection or sterilization cleaning/rinsing. The keyword "dental clean" was used for the Web search. We found the present official definition of instrument cleaning performed prior to disinfection or sterilization rather limiting ("removal of foreign matter (soil, organism, etc.) from the instruments"). Thus, we proposed to expand the definition to include the removal of oils applied to protect the metallic instruments and from corrosion, stains, and rust resulting from the frequent reuse of the instruments. Clinicians are found to clean their dental instruments (a) immediately after treating their patients or (b) following their treatment but not immediately afterward. In the latter case, we recommend presoaking to be added. Ultrasonic sterilization of 5~15 minutes is found to be more effective in terms of eliminating residual matter from the instruments compared to other methods. To check on the cleaning results, we recommend visual inspection, which can be quick and practical in clinical settings. The latest products being developed and marketed on the market address the related problems. Nonetheless, research must be continued on the effects of presoak, cleaning/rinsing, disinfection, and high-temperature or heating-based sterilization on the dental instruments and on dental clinicians' practices in cleaning, disinfection, and sterilization. We advise dental clinicians to select the proper cleaning methods and detergents for their instruments to help eliminate or prevent corrosion, staining, and rusting, to reduce the maintenance costs, and to ensure user-friendly instruments/apparatuses.

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Establishment of some Conditions for the Development of Legume Inoculant (근류균 접종제 개발을 위한 우수 증량제의 선발 및 생산 최적조건)

  • Kim, Chang-Jin;Rhee, Yoon;Yoo, Ick-Dong
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.2
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    • pp.146-151
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    • 1990
  • The viability of rhizobia according to various kinds of carrier materials, inoculum size, storage temperature and sterilization methods was investigated for the development of legume inoculat. The results were followings. 1. Peat and perlite were favorable as a carrier material. 2. Rhizobia counts were reached to $5{\times}10^8cells/g$ carrier 1-2 weeks after inoculation with inoculum size below $10^4cells/g$ carrier. 3. $10^9cells/g$ carrier was maintained 12 weeks after storage at room temperature. 4. Steam heat sterilization was the best method for carrier sterilization among methods used in this study. Dry heat and ${\gamma}$-ray sterilization were also applicable.

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Effect of Microbial Treatment Methods on Biogrout (미생물 처리 방법이 바이오그라우트에 미치는 영향)

  • Kim, Daehyeon;Park, Kyungho;Kim, Hochul;Lee, Yonghee
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.5
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    • pp.51-57
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    • 2012
  • The purpose of study is to understand the possibility of biogrout of soil induced by bacteria. Microbial Calcium Carbonate Precipitation(MCP) has been analysed using the microorganism Bacillus Pasteurii. In order to understand the biogrout of soft ground treated with microbial calcium carbonate precipitation, four types of specimens(sterilization soil, non-sterilization soil, reaction solution and microorganism solution with pre-treatment mix and reaction solution and microorganism solution with post-treatment mix) were made. Scanning Electron Microscope(SEM), EDX and X-ray diffraction(XRD) analyses were performed on the soft ground specimens. On the basis of the preliminary results, it appears that microbial treatment methods using calcium carbonate precipitation may be possible to improve property of biogrout.

Effects of microorganism density and mushroom yields according to the sterilization of casing soils at the cultivation of button mushrooms (복토살균 조건에 따른 양송이 재배과정별 복토내 미생물 밀도 및 수량 특성)

  • Lee, Chan-Jung;Yoo, Young-Mi;Jhune, Chang-Sung;Cheong, Jong-Chun;Moon, Ji-Won;Kong, Won-Sik;Suh, Jang-Sun;Kim, Yong-Gyun;Lee, Byung-Eui;Yoon, Min-Ho
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.220-225
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    • 2014
  • This study was conducted to set the proper sterilization standards of casing soil for the stable production of button mushroom(Agaricus bisporus) from mushroom disease that occurs in infection of casing soil material. Changes of aerobic bacteria are increased as the longer grow-out period and sharply increased after second flushes. Fluorescence Psuedomonas showed high density at high sterilization temperature and $100^{\circ}C$ treatment has extremely high density at 30 min and 60 min in casing 22 days. Density of thermophilic actinomyces is sharply increase from casing with soil and the highest density at 22 days of casing and rapidly decrease after first flushes. Sterilizing temperature of casing soil affects quality and quantity of button mushroom. Treatment of 60 min, 90 min at $80^{\circ}C$ and 30 min at $100^{\circ}C$ produced the highest mushroom yields, especially mushrooms yields of A grads were the highest at treatment of 90 min at $80^{\circ}C$. Treatment of 60min at $100^{\circ}C$ products many yields, however, this treatment has low economic feasibility for its yields. Sterilizing temperature of casing soil has an effect on generating diseases and insect pests. Treatment of 60 min, 90 min at $80^{\circ}C$ and 30 min $100^{\circ}C$ showed lower incidence than the other treatment. Although treatment of 30 min at $100^{\circ}C$ causes low diseases and mushroom fly damage, it has low mushroom yields. Furthermore, although treatment of 60 min at $100^{\circ}C$ has high mushroom yields, it causes high diseases and mushroom fly damage. Therefore the best conditions for the sterilization of casing soils was 60 min and 90 min at $80^{\circ}C$.

Principles and Skills of Organic Crop Production with special regards to Germany (유기작물재배의 이론 및 핵심기술 -독일을 중심으로-)

  • 손상목
    • Korean Journal of Organic Agriculture
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    • v.9 no.4
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    • pp.71-93
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    • 2001
  • Within the paper, an overview of organic farming in Europe countries is given and the Principle and skills of organic agriculture is shortly reported with special regard to Germany. The overview information on European organic forming is covered such as \circled1 development of organic farming, \circled2organic farming organizations, \circled3standards and certification, \circled4implementation of EU council regulation, \circled5state support, \circled6implementation of Agenda 2000, \circled7training and education, \circled8advisory service and research situation. In the paper the principle and skills for organic farming which are practiced actually in the German organic farms is also reported. How to maintain and increase the fertility and microbiological activity of the soil by \circled1cultivation of legumes, green manures or deep-rooting crops in multi-annual rotation system, \circled2incorporation in the soil organic material, by-products from livestock farming is one of the major principle to organic crop production. Pest and diseases and weeds are controlled by any one, or a combination of the following measure ; \circled1choice of appropriate species and varieties, \circled2appropriate rotation programs, \circled3mechanical cultivation, \circled4protection of natural enemies of pests through provision of favourable habitat and ecological buffer zone, \circled5diversified ecosystems, \circled6flame weeding, \circled7natural enemies, \circled8bio-dynamic preparations, \circled9mulching and mowing, \circled10grazing of animals, ⑪mechanical controls, ⑫steam sterilization.

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