• Title/Summary/Keyword: Soil sampling

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Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development

  • Park, Jun-Kyung;Lee, Seung-Hwan;Lee, Jang-Hoon;Han, Songhee;Kang, Hunseung;Kim, Jin-Cheol;Kim, Young Cheol;McSpadden Gardener, Brian
    • The Plant Pathology Journal
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    • v.29 no.2
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    • pp.144-153
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    • 2013
  • Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.

Studies on the Mechanical Properties of Weathered Granitic Soil -On the Elements of Shear Strength and Hardness- (화강암질풍화토(花崗岩質風化土)의 역학적(力學的) 성질(性質)에 관(關)한 연구(硏究) -전단강도(剪斷强度)의 영향요소(影響要素)와 견밀도(堅密度)에 대(對)하여-)

  • Cho, Hi Doo
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.16-36
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    • 1984
  • It is very important in forestry to study the shear strength of weathered granitic soil, because the soil covers 66% of our country, and because the majority of land slides have been occured in the soil. In general, the causes of land slide can be classified both the external and internal factors. The external factors are known as vegetations, geography and climate, but internal factors are known as engineering properties originated from parent rocks and weathering. Soil engineering properties are controlled by the skeleton structure, texture, consistency, cohesion, permeability, water content, mineral components, porosity and density etc. of soils. And the effects of these internal factors on sliding down summarize as resistance, shear strength, against silding of soil mass. Shear strength basically depends upon effective stress, kinds of soils, density (void ratio), water content, the structure and arrangement of soil particles, among the properties. But these elements of shear strength work not all alone, but together. The purpose of this thesis is to clarify the characteristics of shear strength and the related elements, such as water content ($w_o$), void ratio($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$), and the interrelationship among related elements in order to decide the dominant element chiefly influencing on shear strength in natural/undisturbed state of weathered granitic soil, in addition to the characteristics of soil hardness of weathered granitic soil and root distribution of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands. For the characteristics of shear strength of weathered granitic soil and the related elements of shear strength, three sites were selected from Kwangju district. The outlines of sampling sites in the district were: average specific gravity, 2.63 ~ 2.79; average natural water content, 24.3 ~ 28.3%; average dry density, $1.31{\sim}1.43g/cm^3$, average void ratio, 0.93 ~ 1.001 ; cohesion, $ 0.2{\sim}0.75kg/cm^2$ ; angle of internal friction, $29^{\circ}{\sim}45^{\circ}$ ; soil texture, SL. The shear strength of the soil in different sites was measured by a direct shear apparatus (type B; shear box size, $62.5{\times}20mm$; ${\sigma}$, $1.434kg/cm^2$; speed, 1/100mm/min.). For the related element analyses, water content was moderated through a series of drainage experiments with 4 levels of drainage period, specific gravity was measured by KS F 308, analysis of particle size distribution, by KS F 2302 and soil samples were dried at $110{\pm}5^{\circ}C$ for more than 12 hours in dry oven. Soil hardness represents physical properties, such as particle size distribution, porosity, bulk density and water content of soil, and test of the hardness by soil hardness tester is the simplest approach and totally indicative method to grasp the mechanical properties of soil. It is important to understand the mechanical properties of soil as well as the chemical in order to realize the fundamental phenomena in the growth and the distribution of tree roots. The writer intended to study the correlation between the soil hardness and the distribution of tree roots of Pinus rigida Mill. planted in 1966 and Pinus rigida ${\times}$ taeda in 199 to 1960 in the denuded forest lands with and after several erosion control works. The soil texture of the sites investigated was SL originated from weathered granitic soil. The former is situated at Py$\ddot{o}$ngchangri, Ky$\ddot{o}$m-my$\ddot{o}$n, Kogs$\ddot{o}$ng-gun, Ch$\ddot{o}$llanam-do (3.63 ha; slope, $17^{\circ}{\sim}41^{\circ}$ soil depth, thin or medium; humidity, dry or optimum; height, 5.66/3.73 ~ 7.63 m; D.B.H., 9.7/8.00 ~ 12.00 cm) and the Latter at changun-long Kwangju-shi (3.50 ha; slope, $12^{\circ}{\sim}23^{\circ}$; soil depth, thin; humidity, dry; height, 10.47/7.3 ~ 12.79 m; D.B.H., 16.94/14.3 ~ 19.4 cm).The sampling areas were 24quadrats ($10m{\times}10m$) in the former area and 12 in the latter expanding from summit to foot. Each sampling trees for hardness test and investigation of root distribution were selected by purposive selection and soil profiles of these trees were made at the downward distance of 50 cm from the trees, at each quadrat. Soil layers of the profile were separated by the distance of 10 cm from the surface (layer I, II, ... ...). Soil hardness was measured with Yamanaka soil hardness tester and indicated as indicated soil hardness at the different soil layers. The distribution of tree root number per unit area in different soil depth was investigated, and the relationship between the soil hardness and the number of tree roots was discussed. The results obtained from the experiments are summarized as follows. 1. Analyses of simple relationship between shear strength and elements of shear strength, water content ($w_o$), void ratio ($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$). 1) Negative correlation coefficients were recognized between shear strength and water content. and shear strength and void ratio. 2) Positive correlation coefficients were recognized between shear strength and dry density. 3) The correlation coefficients between shear strength and specific gravity were not significant. 2. Analyses of partial and multiple correlation coefficients between shear strength and the related elements: 1) From the analyses of the partial correlation coefficients among water content ($x_1$), void ratio ($x_2$), and dry density ($x_3$), the direct effect of the water content on shear strength was the highest, and effect on shear strength was in order of void ratio and dry density. Similar trend was recognized from the results of multiple correlation coefficient analyses. 2) Multiple linear regression equations derived from two independent variables, water content ($x_1$ and dry density ($x_2$) were found to be ineffective in estimating shear strength ($\hat{Y}$). However, the simple linear regression equations with an independent variable, water content (x) were highly efficient to estimate shear strength ($\hat{Y}$) with relatively high fitness. 3. A relationship between soil hardness and the distribution of root number: 1) The soil hardness increased proportionally to the soil depth. Negative correlation coefficients were recognized between indicated soil hardness and the number of tree roots in both plantations. 2) The majority of tree roots of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands distributed at 20 cm deep from the surface. 3) Simple linear regression equations were derived from indicated hardness (x) and the number of tree roots (Y) to estimate root numbers in both plantations.

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Evaluation of Ecological Function of Mangrove Soil on Absorbing Heavy Metals: A Case Study from the Dongzhaigang Mangrove in China

  • Xin, Kun;Huang, Xing;Zhou, Qingqing;Chen, Zhili
    • Journal of Ecology and Environment
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    • v.33 no.1
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    • pp.15-18
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    • 2010
  • Mangroves are special plant communities that live along intertidal zones in tropical and subtropical areas. They are regarded as one of the most important types of natural ecosystem in the world because of the many ecosystem functions that they perform, of which water purification is the most complex. Mangrove ecosystems are conducive to the deposition and retention of heavy metals. So it is important to understand the impact of heavy metals on mangrove ecosystems, and especially on soil subsystems. We examined the levels of heavy metals in the soil of mangroves in the Dongzhaigang Mangrove National Nature Reserve. Dongzhaigang, the first mangrove nature reserve established in China, is located south of Haikou in Hainan Island and encompasses $33.37\;km^2$, of which mangroves comprise $20.56\;km^2$. To assess the impact of human activities, we collected a large number of soil samples in four sampling areas (the protection station, the harbor, a tour area, and Yeboluo island) in the study area. We measured the concentrations of Cu, Pb, Zn and Cd in the soil samples using the spectra of polyatomic molecules. The average concentrations of Cu, Pb, Zn and Cd were $5.04\;{\mu}g/g$, $10.36\;{\mu}g/g$, $20.06\;{\mu}g/g$ and $0.06\;{\mu}g/g$, respectively, and the heavy metal concentrations were lowest in the protected area, highest in the harbor, and intermediate in Yeboluo Island and the tour area. The heavy metal concentrations in the soil collected from different sample plots are related not only to the physical and chemical properties of the soil, but also to the heavy metal emitted by nearby pollution sources. Our analysis indicates that tourist boats are the main pollution sources in the study area.

Measurements of Gases Emissions form Agricultural Soils and Their Characteristics with Chamber Technique: Emissions of NO and $N_2O$ (챔버를 이용한 농작지로부터의 기체배출량의 측정과 배출특성연구: 일산화질소(NO)와 아질산가스($N_2O$)의 배출량산정)

  • 김득수
    • Journal of Korean Society for Atmospheric Environment
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    • v.17 no.2
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    • pp.203-212
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    • 2001
  • During the growing season from June to August, 2000, the soil NO and $N_2$O fluxes were measured to elucidate characteristics of soil nitrogen emissions from different types of intensively managed agricultural soils at outskirts of Kunsan City, located in the western inland of Korea, Flux measurements were made using a closed chamber technique at two different agricultural fields; one was made from upland field, and the other from rice paddy field. The flux data from upland field were collected for both the green onion and soybean field. Concentrations of NO and $N_2$O inside a flux chamber ar 15 minute sampling interval were measured to determine their soil emissions. Either polyethylene syringes of teflon air bags were used for gas samples of $N_2$O and NO. The analysis of NO and $N_2$O was made using a chemiluminesence NO analyzer and GC-ECD, respectively no later than few hours after sample collection at laboratory. The gas fluxes were varied more than one standard deviation around their means. Relatively high soil gas emissions occurred in the aftermoon for both NO and $N_2$O. A sub-peak for $N_2$O emission was observed in the morning period, but not in the case of NO. NO emissions from rice paddy field were much less than those from upland site. It seems that water layer over the rice paddy field prevents gases from escaping from the soil surface covered with were during the irrigation and acts as a sink of these gases. The NO fluxes resulted from these field experiments were compared to those from grass soil and they were found to be much higher. Diurnal and daily variations of NO and $N_2$O emission were discussed and correlated with the effects of nitrogen fertilizer application on the increase of the level of soil nitrogen availability.

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An Analysis of the Relationship of Soil Factors to the Height Growth of Pinus densiflora within the Young Natural Stands in Central Korea (중부한국의 자연생 소나무의 연 신장성장율에 영향을 미치는 토양요인들에 대한 다요인 분석)

  • 오계칠
    • Journal of Plant Biology
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    • v.15 no.4
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    • pp.1-12
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    • 1972
  • To study on the annual height growth of Pinus densiflora within natural pine stands in central Korea, twenty two pure closed Pinus densiflora stands were selected subjectively in the west-central region of Korea. In each stand twenty trees were chosen randomly. For each tree, abotu ten to fifteen measurements of internodal lengths were made from leader top to trunk base. A total of one hundred thirty four soil samples was collected. Each soil sample was bulked with three subsamples. The ranges of the growth measurements per stand, per tree and per observation were 14.9-35.4cm, 9.0cm-54.4cm and 2.4cm-69.0cm respectively. The total mean value was 23.5cm. The Student-Newman-Keul's tests for the multiple comparison among the mean values of the height growth per stand were very highly significant. The resutls of the analysis of variance of the height growth data for the selected fifteen stands among the twenty two stands indicate that sampling efficiency might be increased to 744% if measurement of the growth were made on fifteen trees per stand from twenty stands instead of twenty trees per stand from fifteen stands. The annual height growths of Pinus densiflora and Pinus koraiensis for the period from 1960 to 1968 were 21.74$\pm$5.29cm (10) and 20.56$\pm$5.59cm (10) respectively. The total means of easily-soluble phosphorus, total nitrogen, loss on ignition and pH for the soil samples were 2.8 ppm, 0.09%, 5.4% and 4.7 respectively. The ranges of those amounts were 18.7-1.7ppm, 0.17-0.05%, 11.6%-3.1%, 3.9-5.1 respectively. The relationship of the annual height growth of P. densiflora to soil was studied in terms of standard partial multiple regression. Among soil properties such as non-capillary pore space, capillary pore space, maximum field capacity, loss on ignition, soil reaction, total nitrogen and easily-soluble phosphoros investigated, the easily soluble phosphorus in one analysis and loss on ignition and soil reaction in the other analysis seem to have significant positive influence on the annual height growth.

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Soil properties of cultivation sites for mountain-cultivated ginseng at local level

  • Kim, Choonsig;Choo, Gap Chul;Cho, Hyun Seo;Lim, Jong Teak
    • Journal of Ginseng Research
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    • v.39 no.1
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    • pp.76-80
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    • 2015
  • Background: Identifying suitable site for growing mountain-cultivated ginseng is a concern for ginseng producers. This study was conducted to evaluate the soil properties of cultivation sites for mountain-cultivated ginseng in Hamyang-gun, which is one of the most well-known areas for mountain-cultivated ginseng in Korea. Methods: The sampling plots from 30 sites were randomly selected on or near the center of the ginseng growing sites in July and August 2009. Soil samples for the soil properties analysis were collected from the top 20 cm at five randomly selected points. Results: Mountain-cultivated ginseng was grown in soils that varied greatly in soil properties on coniferous, mixed, and deciduous broad-leaved stand sites of elevations between > 200mand < 1,000 m. The soil bulk density was higher in Pinus densiflora than in Larix leptolepis stand sites and higher in the < 700-m sites than in > 700-m sites. Soil pH was unaffected by the type of stand sites (pH 4.35-4.55), whereas the high-elevation sites of > 700mwere strongly acidified, with pH 4.19. The organic carbon and total nitrogen content were lower in the P. densiflora stand sites than in the deciduous broad-leaved stand sites. Available phosphorus was low in all of the stand sites. The exchangeable cationwas generally higher in the mixed and low-elevation sites than in the P. densiflora and high-elevation sites, respectively. Conclusion: These results indicate that mountain-cultivated ginseng in Korea is able to grow in very acidic, nutrient-depleted forest soils.

Water Saving Irrigation Manual of Spring Chinese Cabbage (봄배추의 물 절약형 관개기준 설정)

  • Eom, Ki-Cheol;Jung, Pil-Kyun;Koh, Mun-Hwan;Kim, Sang-Hee;Yoo, Sung-Yung;Park, So-Hyun;Hur, Seung-Oh;Ha, Sang-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.812-822
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    • 2010
  • Water management is the most important and difficult problems in crop cultivation. The farmers are doing irrigation, those when to irrigate and how much to irrigate, depending on their experiences. The irrigation manual as water saving is possible, those irrigation interval and amount of irrigation, are developed based on the lysimeter experiments carried out by the RDA for 11 years about potential evapotranspiration, crop coefficient. The manual can be used with easy to the farmer without soil sampling and any kinds of sensors measuring soil water status.

A Study on Isolation of Mixed Heavy Metal-Contaminated Soil and the Waste in Railroad Workshop (철도 정비창의 폐기물과 혼합된 중금속 오염토 분리에 관한 연구)

  • Son, Woohwa;Lee, Seungho
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.12
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    • pp.59-66
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    • 2012
  • In this study, it was sampling from heavy metal-contaminated soil with the waste in railroad workshop. And, the pollution concentration and analysis of particle-size distribution were conducted to design efficient purification process that it was aimed at high contaminated area, low contaminated area and samples containing waste foundry sand. But, it was the other signs of general soil contamination, as construction waste of waste concrete and waste wood, waste foundry sand, incinerator ash, etc is overall buried on the grounds. Thus, the common heavy metal purification technology has not decreased the pollution. However, heavy-metal contamination was reduced by magnetic separation utilizing the magnetic component of the mixed waste.

Natural frequency of bottom-fixed offshore wind turbines considering pile-soil-interaction with material uncertainties and scouring depth

  • Yi, Jin-Hak;Kim, Sun-Bin;Yoon, Gil-Lim;Andersen, Lars Vabbersgaard
    • Wind and Structures
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    • v.21 no.6
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    • pp.625-639
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    • 2015
  • Monopiles have been most widely used for supporting offshore wind turbines (OWTs) in shallow water areas. However, multi-member lattice-type structures such as jackets and tripods are also considered good alternatives to monopile foundations for relatively deep water areas with depth ranging from 25-50 m owing to their technical and economic feasibility. Moreover, jacket structures have been popular in the oil and gas industry for a long time. However, several unsolved technical issues still persist in the utilization of multi-member lattice-type supporting structures for OWTs; these problems include pile-soil-interaction (PSI) effects, realization of dynamically stable designs to avoid resonances, and quick and safe installation in remote areas. In this study, the effects of PSI on the dynamic properties of bottom-fixed OWTs, including monopile-, tripod- and jacket-supported OWTs, were investigated intensively. The tower and substructure were modeled using conventional beam elements with added mass, and pile foundations were modeled with beam and nonlinear spring elements. The effects of PSI on the dynamic properties of the structure were evaluated using Monte Carlo simulation considering the load amplitude, scouring depth, and the uncertainties in soil properties.

Distribution Levels of Phthalates and Di-2-ethylhexyladipate in Domestic Environment (국내 환경 중 프탈레이트 및 디에틸헥실아디페이트 잔류분포 특성)

  • Shin, Hye-Seoung;Shin, Jeoung Hwa;Ahn, Yun Gyong
    • The Korean Journal of Community Living Science
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    • v.23 no.4
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    • pp.411-418
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    • 2012
  • Phthalates and di-2-ethylhexyladipate are both widely used as industrial chemicals and exposure high levels over long periods of time can cause toxicity, estrogenic responses and endocrine disruption properties in both humans and animals. So far, their global monitoring in environmental matrices has been performed continuously. A developed method, including modified SPEED'98 (Japan Environment Agency) and USEPA was established for simple and rapid determination of phthalates and di-2-ethylhexyladipate in various matrices. This method was applied to explore the distribution levels in domestic environmental media such as water, soil and sediment. Eight phthalates (DEP(di-ethyl), DEHP(di-[2-ethyl-hexyl]), DprP (di-propyl), DBP(di-n-butyl), DPP(di-n-pentyl), DHP(di-n-hexyl), DCHP(di-cyclohexyl), BBP(butyl benzyl) and Adipate (di-2-ethylhexyl adipate) were investigated by seasonal sampling(spring, autumn) at 24 domestic sites. Phthalates and adipate were not detected in water samples and DEP, DBP, and DEHP were mainly detected in soil and sediment samples. The concentrations of DEP and DBP excluding DEHP in spring were higher in soil than those of sediment. Total concentrations of phthalates were significantly decreased in autumn for both soil and sediment.