• 제목/요약/키워드: Soil reaction

검색결과 832건 처리시간 0.024초

PCR 기법을 이용한 인공감염토양 및 감염동물 장기로 부터 Bacillus anthracis의 검출 (Establishment of PCR to detect Bacillus anthracis in the experimentally infected soil and mice)

  • 이지연;유한상;김종염
    • 대한수의학회지
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    • 제38권3호
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    • pp.574-580
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    • 1998
  • Anthrax caused by Bacillus anthracis is one of the most important zoonotic diseases in the worldwide. To control and prevent the disease effectively, several methods such as development of a fast and specific diagnostic method and vaccine, education etc, have been carried out. However, it still has a problem in the control and prevention. To control, the most important method is the prevention of direct or indirect contact of the causative agent with susceptible host. Therefore, we developed a fast and specific detection method, polymerase chain reaction, of B anthracis from soil and infected animals because the organism could survive long time in the environment including soil due to formation of spore. With the method, virulence genes of B anthracis were successfully amplified from experimentally infected soil and mice. Up to $4.2{\times}10$ of the organisms per gram could be detected with the PCR method from experimentally infected soil. These results suggested that this PCR method could be effectively used not only to detect B anthracis in soil and infected animal but also to provide the information to prevent the disease.

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Spud-can penetration depending on soil properties: Comparison between numerical simulation and physical model test

  • Han, Dong-Seop;Kim, Moo-Hyun
    • Ocean Systems Engineering
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    • 제7권2호
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    • pp.107-120
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    • 2017
  • Spud-can is used for fixing jack-up rig on seabed. It needs to be inserted up to the required depth during the installation process to secure enough soil reaction and prevent overturning accidents. On the other hand, it should be extracted from seabed soils as fast as possible during the extraction process to minimize the corresponding operational cost. To achieve such goals, spud-can may be equipped with water-jetting system including monitoring and control. To develop such a smart spud-can, a reliable numerical simulation tool is essential and it has also to be validated against physical model tests. In this regard, authors developed a numerical simulation tool by using a commercial program ANSYS with extended Drucker-Prager (EDP) formula. Authors also conducted small-scale (1/100) physical model tests for verification and calibration purpose. By using the numerical model, a systematic parametric study is conducted both for sand and K(kaolin)-clay with varying important soil parameters and the best estimated soil properties of the physical test are deduced. Then, by using the selected soil properties, the numerical and experimental results for a sand/K-clay multi-layer case are cross-checked to show reasonably good agreement. The validated numerical model will be useful in the next-stage study which includes controllable water-jetting.

광촉매 반응에 의한 환경호르몬 분해특성

  • 박재홍;안상우;장순웅
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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    • pp.358-361
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    • 2004
  • The photocatalytic degradation of Endocrine Discruptors, dibuthyl phthalate(DBP) has been investigated over TiO$_2$ photocatalysts irradiated with a ultraviolet (UV) light. The effect of operational parameters, i.e., reaction time, light intensity, pH and additive on the degradation rate of aqueous solution of Endocrine Discruptors has been examined. Results show that the employment of efficient photocatalysts and the selection of optimal operational parameters may lead to degradation of Endocrine Discruptors solutions.

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A Mathematical Model Development for the Nitrification-Denitrification Coupled Process

  • 이미선;이강근
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2003년도 총회 및 춘계학술발표회
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    • pp.430-433
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    • 2003
  • Nitrogen pollution in urban and rural groundwater is a common problem and poses a major threat to drinking water supplies based on groundwater. In this work, the kinetics of nitrification-denitrification coupled reactions are modeled and new reaction modules for the RT3D code describing the fate and transport of nitrogen species, dissolved oxygen, dissolved organic carbon, and biomass are developed and tested. The proposed nitrogen transformations and transport model showed very good match with the results of other public codes.

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Effects of Organic Acids on Availability of Phosphate and Growth of Corn in Phosphate and Salts Accumulated Soil

  • Kim, Myung-Sook;Park, Seong-Jin;Lee, Chang-Hoon;Yun, Sun-Gang;Ko, Byong-Gu;Yang, Jae E.
    • 한국토양비료학회지
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    • 제49권3호
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    • pp.265-270
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    • 2016
  • Accumulated Phosphate can be released by ligand exchange reaction of organic acids. The objective of this study was to evaluate effects of the organic acids on the availability of phosphate and the growth of crop in phosphate and salts accumulated soil. Soil samples were collected from farmer's plastic film house. Available phosphate and electrical conductivity of soil were $3,005mg\;kg^{-1}$ and $16.63mg\;kg^{-1}$ which were 6 and 8 times higher than the optimum range of soil for crop growth, respectively. Corns were cultivated in pots for 2 months. Treatments were no treatment (control), phosphate fertilizer (P), citric acid (CA) 1, 5, 10 mM, and oxalic acid (OA) 1, 5, 10 mM. Water soluble phosphorus, available phosphate, corn growth and uptake were determined after cultivation. Results showed that organic acids increased water soluble phosphorus and available phosphate. For the level of 10 mM, the order of effectiveness of organic acids for water soluble P was citric acid (44%) > oxalic acid (32%). Height and dry weight of corns were increased significantly by the treatment of citric acid 1 and 5 mM. Also, corn absorbed more phosphorus, nitrogen, potassium, calcium and magnesium in the treatment of citric acid 1 mM than these of other treatments. Even though phosphate availability of soil was enhanced by addition of citric acid 10 mM, the growth of corns decreased because high concentration of citric acid caused salt damage by increasement of electrical conductivity. Thus, the citric acid of 1 mM has the potential to improve the availability of phosphate and the healthy growth of corns.

난분해 PAHs의 화학적산화에 의한 유사기질동시대사 (Analogue Substrate Cometabolism by Chemical Oxidation of Recalcitrant PAHs)

  • 류선정;박갑성
    • 한국토양환경학회지
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    • 제3권3호
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    • pp.87-92
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    • 1998
  • The effect of chemically oxidized intermediates of Polynuclear Aromatic Hydrocarbon (PAH) compounds on the degradation of the parent PAHs was characterized and evaluated for the context of cooxidation. Anthracene and pyrene exhibited extensive degradation (mean percent removal of 57.5%) after 28 days of incubation by introducing the Fenton oxidation intermediate of the PAH compounds, while unoxidized anthracene and pyrene exhibited 12.5% removal The chemical oxidation products can serve as a structually similar analogue substrates for a consortia of soil microorganisms and as a metabolic intermediates in the biodegradation sequence of the parent PAH compounds. These results may be interpreted in the context of cooxidation mechanism whereby high recalcitrant PAH compounds are biodegraded in the soil and suggest a potential tool for bioremediation of PAHs contaminated soils and protection of groundwater.

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Strength Characteristics of Stabilized Dredged soil and Correlation with Index Properties

  • 김윤태;도탕하이;강효섭
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2010년도 춘계 학술발표회
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    • pp.489-494
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    • 2010
  • A geo-composite soil (GCS) is a stabilized mixture of bottom ash, cement and dredged soil. Various samples with different mass ratios of mixtures were tested under curing time of 7 and 28 days to investigate physical properties and compressive strength. This paper focused on the effect of bottom ash on the strength characteristics of Busan marine dredged soil. Cement has been added as an additive constituent to enhance self-hardening of the blended mixture. The unconfined compressive strength of GCS increases with an increase in curing time due to pozzolanic reaction of the bottom ash. The strength after 28 days of curing is found to be approximately 1.3 to 2.0 times the strength after 7 days of curing, regardless of mixture conditions. The secant modulus of GCS is in the range of 55 to 134 times the unconfined compressive strength. The correlation of unconfined compressive strength with bottom ash content and initial void ratio are suggested.

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고온공기주입시 지중온도에 의한 ISR의 변화

  • 박기호;박민호;이의신;신항식
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2003년도 추계학술발표회
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    • pp.91-94
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    • 2003
  • A field pilot-scale demonstration of an enhanced SVE using hot air injection and extraction was conducted to remove diesel range compounds from subsurface soils at a site in J-city, Korea. The objective of demonstration was to evaluate field ISR by intrinsic microorganism after an application study of hot air-SVE technology and to calculate each first-order kinetic with soil temperature. TPH concentration of contaminated soil at the site was approximately 2, 000~11, 000 mg/kg (average 6, 900 mg/kg) with depths greater than 5 m bgs. The 1st-order reaction rate constants, k were 0.0438(@about5$0^{\circ}C$), 0.0564(@4$0^{\circ}C$), and 0.0685(@33$^{\circ}C$) d-1 respectively.

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토양안정제에 의한 폐기물 매립장 차수재의 수리전도도 특성

  • 임은진;이재영;이복일
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2002년도 추계학술발표회
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    • pp.390-393
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    • 2002
  • Many researchers have studied for the barrier liner in the landfill that is mixed with clay mineral, native soils and solidified agent. However, they have a littel but problems for safety construction and maintenance as a bottom liner systems in the landfill. In this paper the authors studied the effects on hydraulic conductivity by electric-chemical ion-exchange agent that is a soil stabilization agent(Sulphonated Oil), The application of the soil stabilization agent to meet the hydraulic conductivity of clay liner in landfill is possible if the additive quantity and a proper reaction time is determined relevantly in the laboratory test.

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2차원 토양 실험장치에서 동전기-펜턴 공정의 이해

  • 양지원;박지연;김상준;이유진;기대정
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2003년도 총회 및 춘계학술발표회
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    • pp.310-313
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    • 2003
  • Removal of phenanthrene by electrokinetic method combined with Fenton-like process was studied in a model system. The scale of reactor was 120 cm in length, 10 cm in width, and 50 cm in height. Sand was selected as a model soil. Bentonite was filled in the space between reservoir and contaminated soil to control the flow rate of water. When constant voltage of 100 V was applied to this system, current varied from 1000 mA to 290 mA for 28 days. pH of anode and cathode reservoirs became to 2 and 13, respectively. Removal efficiency of phenanthrene was more than 60 %.

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