• Title/Summary/Keyword: Soil microbial biomass C

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Soil Microbial Diversity of the Plastic Film House Fields in Korea (우리나라 중부지방 시설재배지 토양 미생물의 다양성에 관한 연구)

  • Suh, Jang-Sun;Jung, Beung-Gan;Kwon, Jang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.2
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    • pp.197-203
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    • 1998
  • Although biological metabolism in soil is very important for evaluating the soil properties, most of researches have concerned mainly about physical and chemical sides. In this study, biological characteristics were examined to demonstrate the biota in the plastic film house soils. Contents of organic matter and phosphate in soil were increased with cultivation period. ECs of soil cultivated spinach and melon were $3.59dS\;m^{-1}$ and $3.46dS\;m^{-1}$ respectively: these values were higher than that of rose and flower, which were $1.23dS\;m^{-1}$ and $1.32dS\;m^{-1}$ respectively. The population of fluorescent Pseudomonas strains of the soil cultivated flowers: $113.8{\times}10^4{\sim}129.7{\times}10^4cfu\;g^{-1}$ was higher than that of leafy vegetables: $40.7{\times}10^4{\sim}97.9{\times}10^4cfu\;g^{-1}$ and fruiting vegetables: $25.0{\times}10^4{\sim}91.7{\times}10^4cfu\;g^{-1}$. However the number of Fusarium strains of the soil cultivated with flowers: $3.8{\times}10^2{\sim}4.0{\times}10^2cfu\;g^{-1}$ was lower than that of leafy vegetables: $4.3{\times}10^2{\sim}16.3{\times}10^2cfu\;g^{-1}$ and fruiting vegetables: $7.6{\times}10^2{\sim}30.0{\times}10^2cfu\;g^{-1}$. In relation to the cultivation period, the habitation density of aerobic bacteria, mesophilic Bacillus, thermophilic Bacillus, and fluorescent Pseudomonas strains was the highest in the soil cultivated over 11 years, but diversity index showed negative correlation with cultivation period. Microbial biomass C in these soils had positive correlation with each number of microorganisms including aerobic bacteria, actinomycetes, and strains of mesophilic Bacillus as well as the total number of these microorganisms.

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Soil Chemical Property and Microbial Community under Organic and Conventional Radish Farming Systems (무 유기재배와 관행재배 토양의 화학성과 미생물 군집 비교)

  • Kang, Ho-Jun;Yang, Sung-Nyun;Song, Kwan-Cheol;Cho, Young-Yuen;Kim, Yu-Kyoung
    • Korean Journal of Organic Agriculture
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    • v.27 no.4
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    • pp.479-499
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    • 2019
  • This study was conducted to investigate the responses of soil properties and microbial communities to different agricultural management and soil types, including organic management in Andisols (Org-A), organic management in Non-andisols (Org-NA), conventional management in Andisols (Con-A) and conventional management in Non-andisols (Con-NA) by using a pyrosequencing approach of 16S rRNA gene amplicon in Radish farms of volcanic ash soil in Jeju island. The results showed that agricultural management systems had a little influence on the soil chemical properties but had significant influence on microbial communities. In addition, soil types had significant influences on both the soil chemical properties and microbial communities. Organic farming increased the microbial density of bacteria and biomass C compared to conventional farming, regardless of soil types. Additionally, Org-NA had the highest dehydrogenase activity among treatments, whereas no difference was found between Org-A, Con-A and Con-NA and had the highest species richness (Chao 1) and diversity (Phyrogenetic diversity). Particularly, Chao 1 and Phyrogenetic diversity were increased in organic plots by 12% and 20%, compared with conventional plots, respectively. Also, regardless of agricultural management and soil types, Proteobacteria was the most abundant bacterial phylum, accounting for 21.9-25.9% of the bacterial 16S rRNAs. The relative abundance of putative copiotroph such as Firmicutes was highest in Org-NA plot by 21.0%, as follows Con-NA (13.1%), Con-A (6.7%) and Org-A (5.1%.), respectively and those of putative oligotrophs such as Acidobacteria and Planctomycetes were higher in Con-A than those in the other plots. Furthermore, LEfSe indicated that organic system enhanced the abundance of Fumicutes, while conventional system increased the abundance of Acidobacteria, especially in Non-andisols. Correlation analysis showed that total organic carbon (TOC) and nutrient levels (e.g. available P and exchangeable K) were significantly correlated to the structure of the microbial community and microbial activity. Overall, our results showed that the continuous organic farming systems without chemical materials, as well as the soil types made by long-term environmental factors might influence on soil properties and increase microbial abundances and diversity.

Nutrient Balances and Soil Properties Affected by Application of Soybean and Barley Residues

  • Oh, Taek-Keun;Sonn, Yeon-Kyu;Lee, Dong Sung;Kim, Myung-Sook;Kim, Seok-Cheol;Yun, Hong Bae;Lee, Deog-Bae;Lee, Chang-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.2
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    • pp.120-126
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    • 2014
  • An accurate analysis of nutrient balance in different cropping systems is necessary for improving soil fertilities, causing higher crop yields and quality. This study was carried out to investigate the nutrient balance, changes in soil properties, and their effects on crop yield in long-term field cultivation under mono- and rotation-cropping systems (MCS and RCS, respectively). The analytical results of the soil properties showed that the application of mineral fertilizers alone in the MCS leads the reduction of soil CEC, exchangeable Ca, and microbial biomass C and N. Compared with the MCS of soybean, the RCS of soybean and barley significantly improved the soil properties, which increased crop yield. It might be due to the barley residue added to the RCS soil. Mean nutrient balances for 4 years were -55.9 kg N, +34.7 kg $P_2O_5$, and -0.3 kg $K_2O$ $ha^{-1}$ for the MCS and +19.7 kg N, +107.4 kg $P_2O_5$, and -48.6 kg $K_2O$ $ha^{-1}$ for the RCS, respectively. These nutrient imbalances mean that conventional fertilizer recommendations were inadequate for maintaining soil nutrient balance. From these results, we can conclude that the crop rotation may change comprehensive physical, chemical, and biological soil properties. These changes could affect the nutrient balance and then the crop yield.

Effect of Defective Onion Extract on the Onion Productivity by Organic Farming (양파 파치 추출물이 유기농 양파성에 미치는 영향)

  • Lee, Chun-Hee;Lee, Sang-Dae;Lee, Sung-Ho;Min, Young-bong;Kim, HyeRan;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.1
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    • pp.40-48
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    • 2013
  • This study evaluated the close crop recycling method by using the extract from defective onions. The mixture of the diluted extract (1/500) and nutrients was applied seven times on the onion leaves without spilling it onto the field. The yield and quality of the onions produced by this method (recycle) were compared to those grown organically (control) and conventionally (normal). The yield from the recycling field was increased significantly by 9% compared to that of the control field, while it was decreased by 11% compared to the normal field (p < 0.05). This lower yield was explained by the differences of the mulching vinyl and the fertilizers between the treatments which effect the onion growth during the winter. The content of quercetin in the onions from the recycling filed was increased significantly by 34% and 44% compared to those of the control and normal field, respectively (p < 0.05). It seemed that minerals and biologically active substances in the defective onion extract were effective in increasing the onion growth. In terms of soil microbial biomass, arbuscular mycorrhizal fungi was increased significantly in the recycle field by 40.1% and 30.6% compared to those of the control and normal fields, respectively (p < 0.05). On the other hand, microbial stress (cy19:0/18:1w7c) was decreased in the recycle field by 21.0% and 14.1% compared to those of the control and normal fields, respectively.

Soil Carbon Storage in Upland Soils by Biochar Application in East Asia: Review and Data Analysis (바이오차를 이용한 밭 토양 탄소 저장: 동아시아 지역 연구 리뷰 및 데이터 분석)

  • Lee, Sun-Il;Kang, Seong-Soo;Choi, Eun-Jung;Gwon, Hyo-Suk;Lee, Hyoung-Seok;Lee, Jong-Mun;Lim, Sang-Sun;Choi, Woo-Jung
    • Korean Journal of Environmental Agriculture
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    • v.40 no.3
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    • pp.219-230
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    • 2021
  • BACKGROUND: Biochar is a solid material converted from agricultural biomass such as crop residues and pruning branch through pyrolysis under limited oxygen supply. Biochar consists of non-degradable carbon (C) double bonds and aromatic ring that are not readily broken down by microbial degradation in the soils. Due to the recalcitrancy of C in biochar, biochar application to the soils is of help in enhancing soil carbon sequestration in arable lands that might be a strategy of agricultural sector to mitigate climate change. METHODS AND RESULTS: Data were collected from studies on the effect of biochar application on soil C content conducted in East Asian countries including China, Japan and Korea under different experimental conditions (incubation, column, pot, and field). The magnitude of soil C storage was positively correlated (p < 0.001) with biochar application rate under field conditions, reflecting accumulation of recalcitrant black C in the biochar. However, The changes in soil C contents per C input from biochar (% per t/ha) were 6.80 in field condition, and 12.58 in laboratory condition. The magnitude of increment of soil C was lower in field than in laboratory conditions due to potential loss of C through weathering of biochar under field conditions. Biochar production condition also affected soil C increment; more C increment was found with biochar produced at a high temperature (over 450℃). CONCLUSION: This review suggests that biochar application is a potential measures of C sequestration in agricultural soils. However, as the increment of soil C biochar was affected by biochar types, further studies are necessary to find better biochar types for enhanced soil C storage.

Organic Carbon Distribution and Budget in the Pinus densiflora Forest at Mt. Worak National Park (월악산 소나무림의 유기탄소 분포 및 수지)

  • Lee, Ji-Young;Kim, Deok-Ki;Won, Ho-Yeon;Mun, Hyeong-Tae
    • Korean Journal of Environment and Ecology
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    • v.27 no.5
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    • pp.561-570
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    • 2013
  • Organic carbon distribution and carbon budget of a Pinus densiflora forest in the Songgye valley of Mt. Worak National Park were investigated. Carbon in above and below ground standing biomass, litter layer, and soil organic carbon were measured from May 2011 through April 2012. For the estimation of carbon budget, soil respiration was measured. The amount of carbon allocated to above and below ground biomass was 52.25 and 14.52 ton C $ha^{-1}$. Amount of organic carbon in annual litterfall was 4.71 ton C $ha^{-1}$. Amount of organic carbon within 50cm soil depth was 58.56 ton C $ha^{-1}$ 50cm-$depth^{-1}$. Total amount of organic carbon in this Pinus densiflora forest was estimated to 130.04 ton C $ha^{-1}$. Amount of organic carbon in tree layer, shrub and herb layer was 4.12, 0.10 and 0.04 ton C $ha^{-1}yr^{-1}$ and total amount of organic carbon was 4.26 ton C $ha^{-1}yr^{-1}$. Amount of organic carbon returned to the forest via litterfall was 1.62 ton C $ha^{-1}yr^{-1}$. The amount of carbon evolved through soil respiration was 6.25 ton C $ha^{-1}yr^{-1}$. The amount of carbon evolved through microbial respiration and root respiration was 3.19 and 3.06 ton C $ha^{-1}yr^{-1}$. The amount of organic carbon absorbed from the atmosphere of this Pinus densiflora forest was 1.07 ton C $ha^{-1}yr^{-1}$ when it was estimated from the difference between Net Primary Production and microbial respiration.

Biosorption of Pb and Cd by Indigenous Bacteria Isolated from Soil Contaminated with Oil and Heavy Metals (유류와 중금속으로 오염된 토양에서 분리한 미생물의 Pb와 Cd 생물흡착 특성)

  • Kim, Sang-Ho;Chon, Hyo-Taek;Lee, Jong-Un
    • Economic and Environmental Geology
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    • v.42 no.5
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    • pp.427-434
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    • 2009
  • Indigenous bacterium which shows a tolerance to high metal toxicity was isolated from soil concomitantly contaminated with oil and heavy metals. The characteristics of the bacterium for Pb and Cd biosorption was investigated under the various experimental conditions such as bacterial growth phase, the initial metal concentration, the input biomass amount, temperature and pH. The Langmuir adsorption isotherm modeling was described to know the capacity and intensity of biosorption. The low initial concentration of heavy metals and high biomass has a maximum heavy metal removal efficiency, but biosorption capacity of Pb and Cd has different values. Biosorption efficiency was highest in the end of the microbial growth stage and under pH 5~9 condition, but was less affected by temperature variation of 25~$35^{\circ}C$. The maximum biosorption capacity for Pb and Cd was 62.11 and 192.31 mg/g, respectively and each $R^2$ was calculated as 0.71 and 0.98 by applying Langmuir isothermal adsorption equation. Biosorption for Cd was considered as monomolecular adsorption to single layer on the surface of cells, whereas biosorption for Pb was considered as accumulation process into the cell by the microbial metabolism and precipitation reaction with anion of bacteria.

Valuation of Ecosystem Services through Organic Carbon Distribution and Cycling in the Quercus mongolica Forest at Mt. Worak National Park (월악산 신갈나무림의 유기탄소 분포와 순환을 통한 생태계서비스 가치평가)

  • Won, Ho-Yeon;Shin, Chang-Hwan;Mun, Hyeong-Tae
    • Journal of Wetlands Research
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    • v.16 no.3
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    • pp.315-325
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    • 2014
  • Valuation of ecosystem services through organic carbon distribution and cycling in the Quercus mongolica forest at Mt. Worak national park were investigated from May 2012 through April 2013. The amount of carbon allocated to above and below ground biomass was 81.94 and 20.53 ton C/ha. Amount of organic carbon in litter layer was 6.49 ton C/ha. Amount of organic carbon within 50 soil depth was 141.23 ton C $ha^{-1}$ $50cm-depth^{-1}$. Total amount of organic carbon in this Quercus mongolica forest was estimated to 250.19 ton C $ha^{-1}$. The estimated amount of won in this Quercus mongolica forest in terms of total organic carbon was about 5.27 million won $ha^{-1}$. The amount of carbon evolved through soil respiration was 7.31 ton C $ha^{-1}yr^{-1}$. The amount of carbon evolved through microbial respiration and root respiration was 3.58 and 3.73 ton C $ha^{-1}yr^{-1}$, respectively. The amount of organic carbon absorbed from the atmosphere of this Quercus mongolica forest was 1.61 ton C $ha^{-1}yr^{-1}$ when estimated from the difference between net primary production and microbial respiration. This amount will come to about 33,000 won $ha^{-1}yr^{-1}$ in Korean currency.

Valuation of Ecosystem Services through Organic Carbon Distribution and Cycling in the Pinus densiflora Forest in Mt. Worak National Park (월악산 소나무림의 유기탄소 분포와 순환을 통한 생태계서비스 가치평가)

  • Won, Ho-Yeon;Lee, Young-Sang;Mun, Hyeong-Tae
    • Journal of Wetlands Research
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    • v.17 no.4
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    • pp.332-338
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    • 2015
  • Valuation of ecosystem services through organic carbon distribution and cycling in the Pinus densiflora forest at Mt. Worak National Park were investigated from January 2013 through December 2013. The amount of carbon allocated to above and below ground biomass was 32.17 and 8.04 ton C $ha^{-1}$. Amount of organic carbon in litter layer was 5.55 ton C $ha^{-1}$. Amount of organic carbon within 50cm soil depth was 58.62 ton C $ha^{-1}$ 50cm-$depth^{-1}$. Total amount of organic carbon in this Pinus densiflora forest was estimated to 104.38 ton C $ha^{-1}$. The estimated amount of won in this Pinus densiflora forest in terms of total organic carbon was about 10.44 million won $ha^{-1}$. The amount of carbon evolved through soil respiration was 4.44 ton C $ha^{-1}yr^{-1}$. The amount of carbon evolved through microbial respiration and root respiration was 2.18 and 2.27 ton C $ha^{-1}yr^{-1}$, respectively. The amount of organic carbon absorbed from the atmosphere of this Pinus densiflora forest was 0.44 ton C $ha^{-1}yr^{-1}$ when estimated from the difference between net primary production and microbial respiration. This amount will come to about 44,000 won $ha^{-1}$ in Korean currency.

Effect of Continual Application of Liquid Pig Manure on Malting Barley Growth and Soil Environment in Double Cropping System of Rice-Malting Barley (벼.맥주보리 작부체계에서 돈분액비 연용이 맥주보리 생육과 토양 환경에 미치는 영향)

  • Lee, Seong-Tae;Seo, Dong-Cheol;Kim, Eun-Seok;Song, Won-Doo;Lee, Won-Gyu;Heo, Jong-Soo;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.3
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    • pp.341-348
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    • 2010
  • To investigate the effect of continual pre-plant application of liquid pig manure (LPM) on malting barley growth, quality and soil environment in double cropping system of rice and malting barley, the liquid pig manure was applied after harvesting rice and malting barley for 3 years. Field experiment was designed with non-fertilizer, chemical fertilizer (CF) recommended by soil testing, rice (LPM 50%+CF 50%)+malting barley (CF 100%), rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%), rice (LPM 100%)+malting barley (CF 100%) and rice (LPM 100%)+malting barley (LPM 100%). By continuous application of LPM 100%, the contents of available $P_2O_5$ and exchangeable K in the soil were increased. The available $P_2O_5$ increased from 243 to 350 mg $kg^{-1}$ and exchangeable K was changed 0.31 to 0.44 $cmol_{c}\;kg^{-1}$. However, the contents of available $P_2O_5$ and exchangeable K were not significant changes in rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%) plot. Bulk density of soil was not affected by application of LPM. The microbial density was high in order of bacteria > actinomycetes > fungi. The population of aerobic bacteria in rice (LPM 100%)+malting barley (LPM 100%) plot was higher than other plots. The ratio of aerobic bacteria/fungi and biomass C content were the highest in rice (LPM 100%)+malting barley (LPM 100%) plot. The yield of malting barley was increased 22% by increasing culm length, panicle length, No. of panicle and 1,000 grains as 358 kg $10a^{-1}$ in rice (LPM 100%)+malting barley (LPM 100%) plot compared with 294 kg $10a^{-1}$ in rice (CF 100%)+malting barley (CF 100%) plot. The content of ${\beta}$-glucan was low by 4.5 and 4.4% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot, respectively. The content of crude protein was the lowest by 8.2% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot and the quality of malting barley was good as within 11%.