• Korean Journal of Soil Science and Fertilizer
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• v.21 no.3
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• pp.254-263
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• 1988

Contents and distribution of amino acids in the humic acid and fulvic acid fractions of different types ($R_p$, B, A, P) were investigated. Extracted humic and fulvic acids were purified and analyzed. The results are summarized as the following: (1) Composition of Humus The total humus ($H_T$), amount of humic acid (a), amount of fulvic acid (b), and ${\Delta}logK$ all decrease in the order of $R_p$ > B > A > P type. The same trend was observed in the total nitrogen and carbon. (2) Contents and composition of amino acids in humic acids. 1) The total amounts of amino acids in the humic acid fraction of different types were in the following order for soils under coniferous forest trees: $R_p$ > B > A > P type, but for soils under deciduous forest trees the order was P > A > $R_p$ > B type. There were positive correlationships between total amino acids and total carbon and ${\Delta}logK$ for humic acids from soils under coniferous forest trees, but a negative correlationship was existed. between total amino acids and C/N ratios. No significant correlation was found for samples taken from soils under deciduous forest trees. 2) The ratios of one group of amino acids to the others were compared. The ratios of acidic amino acids were in the order of P > $R_p$ > B > A type. those of neutral amino acids followed the order of $R_p$ > B > A > P type and those of the basic amino acids were in the order of B > A >$R_p$ > P type for soils under coniferous forest trees. Contents of total amino acids were in the order of the neutral > the acidic > the basic amino acids. For the soils under deciduous forest trees the order of the ratio was different. Acidic amino acids followed the order of A > P > B > $R_p$ type, neutral ones followed the order of P > $R_p$ > A > B type, and the basic amino acids did the order of $$P{\geq_-}$$ A > B $$\geq_-$$ $-R_p$ type where the difference was very small. 3) In general aspartic aicd, glycine and glutamic acid were the major components in all samples. Histidine, tyrosine and methionine belonged to the group contained in a small amount. (3) Contents and composition of amino acids in fulvic acids. 1) The total amounts of amino acids of different types of fulvic acids were in the order of $R_p$ > B > P > A type regardless of origin of samples. There were positive correlationships observed between the toal amino acids and total carbon and ${\Delta}logK$ for soils under coniferous forest trees. For soils under deciduous forest trees, positive correlationships were observed among total amino aicds, total nitrogen, total humus ($H_T$), total humic aicd (a), and ${\Delta}logK$, but a negative correlationship existed between total amino acids and C/N ratio. 2) Thr ratio among acidic amino acids, neutral amino acids and basic amino acids of different types were $R_p$ > B > P > A type. In this respect there was no difference between the two soils. 3) In general glycine, aspartic acid, and alanine were the major constituents in all samples of different types, while tyrosine and methionine were contained in a small amount. Virtually no amount of arginine was measured.

• Applied Biological Chemistry
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• v.12
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• pp.115-118
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• 1969

In a series of studies on the formation processes and chemical properties of humic substances from paddy soils the photoabsorption spectra of hymatomelanic and humic acids were recorded in the visible, ultraviolet and infrared regions. The spectra patterns of both fractions in the visible range were indicative of the decrease in optical density with the rise of wavelength and the UV-spectra showed the maximum peak at $235\;m{\mu}$, the minimum at $395\;m{\mu}$. The IR-spectra indicate a typical pattern showing a few sharp peaks in both fractions. The spectra of both fractions were compared and discussed.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.49-53
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• 2006

The possibility of removal of PCBs (Polychlorinated Biphenyls) in soil was studied using biological method. The effects of soil and co-substrate on both PCBs reduction rate and chloride ion concentration in soil were investigated. It was shown that PCBs concentration of all the soils used in this study were reduced from 5ppm to below 1ppm after 60days, also chloride ion concentration in slurry increased, Results showed that leaf mold and humic acid as co-substrate do not seem to be effective for biological treatment of PCBs in soil,

• Applied Biological Chemistry
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• v.38 no.3
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• pp.263-268
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• 1995

A study was undertaken to compare degradation patterns of carbofuran in soils between submerged and upland moisture conditions [$3-^{14}C$]Carbofuran was treated in each soils at the rate of 1.0 mg/kg (87.8 kBq $^{14}C/50g$ soil) and the time-course analysis for distribution of radioactivity and degradation products were conducted. Differences in the pathway and rate of carbofuran degradation in soils were observed between submerged and upland moisture conditiona major degradation being hydrolysis at 7-C position and oxidation at 3-C position, respectively. Carbofuran showed less persistence in soils of higher moisture contents A significant portion, $24{\sim}39%$ of the total radioactivity, resided in soils as nonextractable residues at 60 days after treatment The nonextractable radioactivity was mainly located in soil organic matter, fulvic acid, humic acid and humin factions Gel filtration chromatography confirmed the incorporation of carbofuran and its degradation products into the organic matter.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.4
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• pp.429-439
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• 1999

Potentiometric titration data were collected for some humic acids purified from Korean upland and paddy soils over a range of pH (3.0 - 11.0) with $NaNO_3$ background electrolyte concentrations (0.01, 0.10, 0.50 and 1.00 M). The data were applied to model A and V which included both intrinsic heterogeneity of humic materials and electrostatic interaction influences on binding sites. The elemental analysis were conducted for various type of humic samples. The $E_4/E_6$ ratio proposed negative correlation with the total carboxyl groups ($r^2$= 0.9988). The charge ($cmol_c\;kg^{-1}$) on the humic acids became more negative as the ionic strength increased. In both continuous and batch titrations, the ionic strength effect was greater in Namweon series (pH 6.39) than others at pH 5.00. The effect of ionic strength on surface charge appears to be greater in batch titrations. This could suggest that continuous titrations do not represent an equilibrium state and the effects of electrolyte concentration was not fully realized during the course of titrations. Both models described experimental data obtained from continuous and batch titrations well over a range of ionic strengths. Model A is more simpler than model V but adaptes more fitted parameters. Thus, the observed change in apparent binding constants with surface charge is regarded solely due to electrostatic influences rather than functional group heterogeneity. However, Model V is more mechanistically realistic in a number of discrete ligand binding sites.

• Korean Journal of Soil Science and Fertilizer
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• v.21 no.4
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• pp.416-421
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• 1988

Humic acids were extracted from straw of wheat and rye at three different stages of decomposition. Contents and distribution of amino acids in the hydrolysates of humic acids were examined and the results obtained can be summarized as the following: 1. Contents and distribution of amino acids in the hydrolysates of humic acids differ from plant to plant and from one stage of decomposition to another. 2. Neutral amino acids as a group take the largest portion of the total amino acids in humic acid hydrolysates followed by the acidic and the basic. 3. The total amount of amino acids in decomposed wheat straw at the 90 days of humification was greater than that in the case of rye straw. 4. Contents of amino acids other than arginine, histidine and tyrosine were increased in the case of wheat straw, while only the contents of lysine, phenylalanine, tyrosine and methionine were observed to increase in the case of rye straw. 5. Exceptionally high contents of phenylalanine and tyrosine were measured in the hydrolysate from rye straw taken at the end of experimental period. 6. No amount of arginine was detected in any hydrolysate of humic acids from decomposed plant residues.

• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.561-563
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• 1994

For the more effective isolation of soil actinomycetes, we have developed HHV (Hair hydrolysate-vitamin) agar medium, containing hair as the sole source of carbon and nitrogen. The HHV agar medium was superior to other media such as colloidal chintin agar, glycerol-arginine agar and starch-casein-nitrate agar, and HV (humic acid-vitamin) agar. The maximum effect of this medium has been shown in hair dry weight 0.4 g/l medium. Of each soil sample, the greatestest number of actinomycetes was isolated from the potato annual planted soil among the tested samp- les. The genus of actinomycetes isolated from the potato annual planted soil sample was identified such 5 group as Stretomyces, Micromonospora, Microbispora, Nocardia and Saccharopolyspora.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.775-786
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• 2018

To test the hypothesis that humic acid (HA), anaerobically digested pig slurry filtrate (APS), and their combination would differently affect the chemical speciation and extractability of metals (cadmium, copper, and zinc) and their uptake by plants, we conducted a pot experiment using wormwood in two texturally contrasting soils (sandy loam and clay loam) collected from a field near an abandoned Cu mine. Four treatments were laid out: HA at $ 23.5g\;kg^{-1}$ (HA), APS at $330mL\;kg^{-1}$ (APS), HA at $ 23.5g\;kg^{-1}$ and APS at $330mL\;kg^{-1}$ (HA + APS), and a control. Each treatment affected the chemical speciation and mobility of the metals, and thereby resulting in variable patterns of plant biomass yield and metal uptake. The APS supported plant growth by increasing nutrient availability. HA supported or hindered plant growth by impacting the soil's water and nutrient retention capacity and aeration, in a soil texture-dependent manner, while consistently enhancing the immobilization of heavy metals. Temporal increases in whole-plant dry matter yield and metal accumulation suggested that the plants were capable of metal hyperaccumulation. The results were discussed in terms of the mobility of metals and plant growth and corroborated by the $^{15}N$ recovery of soil- and plant-N pools under H and HS treatments. Therefore, for effective phytoremediation of polluted soils, an appropriate combination of plant growth promoters (APS) and chelating agents (HA) should be predetermined at the site where chemical stabilization of pollutants is desired.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.1
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• pp.26-33
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• 1990

The pot experiment was conducted to compare the effects of BIO-COM (Organic fertilizer made from industrial by-product) with the compost and humic acid on the physico-chemical properties of soils and the yield of paddy rice (CV. Chucheong-byeo). The results obtained were as follows: 1. All the fertilizer treatments, except humic acid, increased rice yield compare to no fertilizer treatment, and Bio-Com was the highest among the fertilizer treatments. 2. Rice yields were increased with increasing BIO-COM only up to 4% but 2% with N.P.K. fertilizers. 3. Rice yield showed highly positive correlation with number of panicle per hill but negative correlation with 1000 grain weight. 4. BIO-COM application induced the noticeable increase of pH, EC, OM, T-N, Av-P, Ex-Ca, Ex-Mg, Ex-Na, and $Av-SiO_2$, contents in the soils after experiments, and there were highly significant positive correlation among them. 5. BIO-COM application was positively affected on plant height, number of tiller, and fresh weight and dry weight of rice plant throughout entire growing stages and these are positively correlated with rice yield. 6. BIO-COM application also induced the increase of chlorophyll, N, P, Ca, and Mg contents in rice plant 7. It is revealed that there were highly positive correlations between rice yields and pH, EC, OM, T-N, Ex-Ca, and Ex-Mg in the soils. 8. It was also shown the highly significant positive correlations between rice yields, and N and P contents in the rice plant at heading stages, respectively.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.11
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• pp.1067-1074
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• 2008

In this study, the organic carbon normalized-sorption coefficients (Koc) for the binding affinity of phenanthrene (PHE) to 16 different soil humic and fulvic acids of various origins were determined by fluorescence quenching. The humic and fulvic acids used in this study were isolated from 6 different domestic soils including Mt. Hanla soil, IHSS standard soil and peat as well as Aldrich humic acid and characterized by elemental composition, ultraviolet absorption at 254 nm, composition of main structural fragments determined by CPMAS $^{13}$C NMR. The Koc values($\times$10$^4$, L/kg C) for each of HA and FA samples were in the range of 1.48$\sim$8.65 and higher in HA compared to that of FA(3.13$\sim$8.65 vs 1.48$\sim$2.48) in the experimental condition([PHE]/[HS] = 0.02$\sim$0.2(mg/L)/(mg-OC/L), pH 6). The correlation study between the structural descriptors of humic and fulvic acids and log Koc values of phenanthrene, show that the magnitude of Koc values positively correlated with the UV$_{254}$ absorptivity([ABS]$_{254}$) and two $^{13}$C NMR descriptors (C$_{Ar-H,C}$, $\sum$C$_{Ar}$/$\sum$C$_{Alk}$), while negatively correlated with the independent descriptors of the(N+O)/C atomic ratios and $^{13}$C NMR descriptors (I$_{C-O}$/I$_{C-H,C}$). These results confirmed that the binding affinity for the hydrophobic organic compound, phenanthrene are significantly influenced by the polarity and aromaticity of soil humc and fulvic acids.