• Korean Journal of Ecology and Environment
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• v.40 no.4
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• pp.537-545
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• 2007

The heavy metal removal capacity of filamentous green alga Cladophora sp. cultured together with wet-mixed solidified soil (loess) was tested. A Cladophora sp. was cultured for 5d, with added Chu No. 10 medium, in stream water contaminated by high concentration of heavy metals from a closed mine effluent. Heavy metal ion concentrations of the medium and in algal tissue were measured every day during the experiment. Dissolved metals (Al, Cd, Cu, Fe, Mn, Zn) in medium were rapidly removed (over 90% elimination) within 1-2d when alga and loess were added. Dissolved heavy metals dropped by only 10% when algae were cultured without loess. The Cladophora sp. accumulated much more heavy metals when cultured with loess than when the alga was cultured alone. Cladophora sp. exhibited a maximum uptake capacity for Al ($17,000{\mu}g^{-1}$ algal dry weight). The metal bioremoval capacities of the algae were in the order Al, Fe, Cu, Mn, Zn and Cd. The heavy metal removal capacity of Cladophora sp. showed significant increases when wet-mixed solidified soil was added to culture media.

• Journal of Soil and Groundwater Environment
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• v.17 no.3
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• pp.49-58
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• 2012

Batch experiments using indigenous and commercialized adventive microorganisms were performed to investigate the feasibility of the biodegradation process for the diesel contaminated soil, which was taken in US Military Camp 'Hialeah', Korea. TPH concentration of the soil was determined as 3,819 mg/kg. Four indigenous microorganisms having high TPH degradation activity were isolated from the soil and by 16S rRNA gene sequence analysis, they were identified as Arthrobacter sp., Burkholderia sp., Cupriavidus sp. and Bacillus sp.. Two kinds of commercialized solutions cultured with adventive microorganisms were also used for the experiments. Various biodegradation conditions such as the amount of microorganism, water content and the temperature were applied to decide the optimal bioavailability condition in the experiments. In the case of soils without additional microorganisms (on the natural attenuation condition), 35% of initial TPH was removed from the soil by inhabitant microorganisms in soil for 30 days. When the commercialized microorganism cultured solutions were added into the soil, their average TPH removal efficiencies were 64%, and 54%, respectively, which were higher than that without additional microorganisms. When indigenous microorganisms isolated from the contaminated soil were added into the soil, TPH removal efficiency increased up to 95% (for Bacillus sp.). According to the calculation of the average biodegradation rates for Bacillus sp., the remediation goal (87% of the removal efficiency: 500 mg/kg) for the soil would reach within 24 days. Results suggested that TPH removal efficiency of biodegradation by injecting indigenous microorganisms is better than those by injecting commercialized adventive microorganisms and only by using the natural attenuation.

• Korean Journal Plant Pathology
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• v.11 no.1
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• pp.39-46
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• 1995

Ditylenchus destructor isolated from diseased ginseng roots was cultured on ginseng calli, fungal mycelium (Fusarium solani), carrot discs and radish sprouts. Effects of temperature, organic material and flooding on the nematode population changes were examined. D. destructor multiplied readily on the culture media except radish sprout medium, and was cultured best on the fungal culture at 2$0^{\circ}C$. Feeding of the fungal hypha and radish root hairs, molting and mating in the fungal culture medium were observed. Addition of organic materials (perilla, sesame, soybean and ginseng leaves) in soil significantly increased Aphelenchus avenae and saprophytic nematode populations, while D. destructor populations changed little and the nematode population growths were limited by the organic amendments (except sesame leaves). The nematode populations in soil including D. destructor were decreased by flooding. The results indicate that D. destructor may survive but not multiply readily in soil without host plants and that it can be effectively controlled by flooding.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.382-388
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• 2002

In order to assess the effects of organic fertilizer on soil microbial community structure and diversity in the greenhouse fields, fatty acid methyl ester (FAME) was analyzed by the MIDI (Microbial ID, Inc., Newark, DE, U.S.A.) system and enumerations were performed. In relation to bacterial division of each sample, low GC Gram-positive bacteria were predominant among bacteria cultured on aerobic bacteria media. On the other hand, alpha subdivision was predominant on proteobacteria of control and OM (organic matter) 1 treated plot, and Flavobacterium spp. existed in OM2 plot on crystal violet media of all samples. Shannon-weaver Index (H) of OM1 plot varied most by 1.9 and 5.0 among bacteria cultured on aerobic bacteria media and crystal violet media, respectively. Our results revealed that addition of the organic wastes to soil led to a highly diverse microbial community, but the excessive amounts of organic and mineral fertilizer applied in the greenhouse fields produced excess nutrients in soil and led to simplification on bacterial populations.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.03b
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• pp.489-495
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• 2000

When microorganisms such as bacteria and fungi are injected into porous medium such as soils along with appropriate substrate and nutrients, biomass retained in the soil pore. Soil pore size and shape are varied from the initial condition as a result of biofilm formation, which make hydraulic conductivity reduced. In this research, hydraulic conductivity reduction was measured after microorganism are inoculated and cultured with synthetic substrates and nutrients. Biomass-soil mixture was evaluated its applicability to the field condition as an alternative liner material in landfill by measuring hydraulic conductivity change after repetitive freeze-thaw cycles.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.2
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• pp.162-167
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• 1983

These studies were carried out to investigate the effects of organic matter on the leaching of chemical constituents in paddy soil. Rice plants were cultured in paddy soil to which rice straw and compost were applied and then chemical properties of percolated water were analysed. The results were as follows: In the paddy soil cultured with rice plants, the pH value of soil was higher in the compost plot than in the rice straw plot. The leaching of iron, managanese, calcium, magnesium and phosphate was increased by cultivation of rice plant, but that of potassium, ammonium, and silica was decreased. When rice straw was applied in the paddy soil, the leaching of iron, managanese, phosphate and silica was increased during the growing periods, while that of calcium, magnesium, potassium and ammonium was increased by application of compost.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.324-328
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• 2008

One hundred-eighty extracts of soil collected from ginseng (Panax ginseng C.A. Meyer) fields were subjected to lettuce germination test, electrolyte leakage, cell viability and antioxidant enzyme activity test. Regardless of various cultivation periods, there was no significant difference in soil pH, the content of organic matter and available phosphate in ginseng fields. Based on lettuce seed germination test, six soil extracts showing inhibition of germination and/or seedling growth were selected for further study. Selected soil extracts markedly inhibited cell viability of ginseng cultured cells but leakage of electrolytes were not affected by the treatment. Enzyme activity of superoxide dimutase in ginseng cultured cells was not affected by the treatment with the soil extracts. However, those of peroxidase and catalase were significantly inhibited by the treatment with soil extracts which showed inhibition of lettuce seed germination and seedling growth.

• Korean Journal of Environmental Biology
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• v.33 no.2
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• pp.215-222
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• 2015

During our investigation of ciliates from Korea, four ciliates were isolated from the non-flood Petri dish culture of soil. They were cultured under laboratory condition and studied using standard methods morphologically, including living observations, protargol impregnations, and morphometrics. The four species are Metopus hasei Sondheim, 1929, Urosomoida agiliformis Foissner, 1982, Oxytricha proximata Shibuya, 1930, and Cladotricha sp. in Nie${\ss}$en (1984) respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.1
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• pp.36-41
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• 1983

These studies were carried out to investigate the effects of potassium salts on the changes of chemical properties in submerged soil. Rice plants were cultured in submerged soil using potassium salts. Obtained results were as follows. In the submerged soil cultured with rice plants the value of pH was higher in the potassium chloride plot than in the potassium sulfate plot. The leaching of cations such as calcium, magnesium, potassium and ammonium were higher in the potassium chloride plot than in the potassium sulfate plot. On the other hand, the leaching of phosphate ion was slightly higher in the potassium sulfate plot than in the potassium chloride plot. The leaching of iron was higher in the planted plot than in the non-planted plot, but that of silica was higher in the non-planted plot than in the planted plot. However, the leaching of iron and silica was not affected by potassium salts.

• Journal of Environmental Science International
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• v.21 no.4
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• pp.451-459
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• 2012

PCDDs(Polychlorinated dibenzo-$p$-dioxins) and PCDFs(polychlorinated dibenzofurans) are measured in soil of Yeungju and Sangju on North Gyeongbuk to investigate the risk assessment of dioxins. Dioxins are analyzed by HRGC/HRMS(high resolution gas chromatography - high resolution mass spectrometer). 2,3,7,8-T4CDD and 1,2,3,7,8-Pe5CDD in soil on Yeungju and Sangju are not detected. Also, 2,3,7,8-T4CDF is not detected in soil on Yeungju and Sangju. PCDDs and PCDFs in Yeungju soil are 1.957 pg/g and 0.294 pg/g, respectively. Total of dioxins in Yeungju soil are 2.251 pg/g. PCDDs and PCDFs in Sangju soil are 1.220 pg/g and 0.420 pg/g, respectively. Total of dioxins in Sangju soil are 1.640 pg/g. PCDDs and PCDFs in Yeungju soil are 0.0049 pg WHO-TEQ/g and 0.0123 pg WHO-TEQ/g, respectively. Total of dioxins with PCDDs and PCDFs in Yeungju soil are 0.0172 pg WHO-TEQ/g. PCDDs and PCDFs in Sangju soil are 0.0065 pg WHO-TEQ/g and 0.0213 pg WHO-TEQ/g, respectively. Total of dioxins with PCDDs and PCDFs in Sangju soil are 0.0278 pg WHO-TEQ/g. Amount for pg/g concentration unit of PCDDs is higher than amount of PCDFs in soil. But, WHO-TEQ of PCDFs is higher than WHO-TEQ of PCDDs in soil.