• Journal of Soil and Groundwater Environment
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• v.26 no.5
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• pp.49-59
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• 2021

The effects of green manure crops, hairy vetch and sesban, supplemented with HS (humic substance) on biological soil health indicators was studied in a pot containing two kinds of reclaimed soil previously contaminated with petroleum hydrocarbons; a soil remediated by land-farming (DDC) and another soil by low-temperature thermal desorption (YJ). Treatments include no plant (C), plants only (H), and plants+2% HS (PH), which were evaluated in a pot containing respective soil. Biological indicators include microbial community analysis as well as soil enzyme activities of dehydrogenase, 𝛽-glucosidase, N-acetyl-𝛽-D-glucosaminidase (NAG), acid/alkaline phosphatase, arylsulfatase, and urease. Results showed an increase of enzyme activities in pot soils with plants and even greater in soils with plants+HS. The enzyme activities of DDC soil with plants (DDC_P) and with plants+HS (DDC_PH) increased 1.6 and 3.9 times on average, respectively than those in the control. The enzyme activities YJ soil with plants (YJ_P) and with plant+HS (YJ_PH) increased 1.8 and 3.8 times on average, respectively than those in the control. According to microbial community analysis, the relative abundance of nitrogen-fixing bacteria in DDC and YJ soil was increased from 1.5% to 7% and from 0 to 5%, respectively, after planting hairy vetch and sesban. This study showed that mixed planting of green manure crops with a supplement of humic substance is highly effective for the restoration of biological health indicators of reclaimed soils.

• Microbiology and Biotechnology Letters
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• v.42 no.1
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• pp.32-40
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• 2014

Methane oxidation and the production potentials of ground soil (soil A) and garden soil (soil B, C, & D) in an urban school were evaluated, and the methanotrophic and methanogen communities in the soil samples were quantified using quantitative realtime PCR. The methanotrophic community in the raw soil A sample possessed a $6.1{\times}10^3$ gene copy number/g dry weight soil, whereas those in the raw soils B~D samples were $1.6-1.9{\times}10^5$ gene copy numbers/g dry weight soil. Serum bottles added with the soil samples were enriched with methane gas, and then evaluated for their methane oxidation potential. The soil A sample had a longer induction phase for methane oxidation than the other soils. However, soil A showed a similar methane oxidation potential with soils B~D after the induction phase. The methanotrophic community in the enriched soil A sample was increased by up to $2.3{\times}10^7$ gene copy numbers/g dry weight soil, which had no significantly difference compared with those in soils B~D ($1.2-2.8{\times}10^8$ gene copy numbers/g dry weight soil). Methane production showed a similar tendency to methane oxidation. The methanogens community in raw soil A ($1.7{\times}10^5$ gene copy number/g dry weight soil) was much less than those in raw soils B~D ($1.3-3.4{\times}10^7$ gene copy numbers/g dry weight soil). However, after methane gas was produced by adding starch to the soils, soil samples A~D showed $10^7$ gene copy numbers/g dry weight soil in methanogens communities. The results indicate that methanotrophic and methanogenic bacteria have coexisted in this urban school's soils. Moreover, under appropriate conditions for methane oxidation and production, methanotrophic bacteria and methanogens are increased and they have the potential for methane oxidation and production.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.2
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• pp.125-131
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• 2016

The biodegradable film application can escape from plastic environmental pollution. This experiments studied the effect of biodegradable mulch film on the soil microbial community using fatty acid methyl ester method in soybean production field. The soil $NO_3$-N content in polyethylene mulch film (PE) soil was significantly higher than biodegradable mulch film soil (p < 0.05). The soil microbial community of Gram negative bacteria showed significantly higher in biodegradable mulch film soil than PE mulch film soil (p < 0.05). In addition, biodegradable mulch film soil had significantly low ratio of cy17:0 to $16:1{\omega}7c$ and cy19:0 to $18:1{\omega}7c$ compared with those of PE mulch film soil (p < 0.05), indicating that microbial stress decreased. The ratio of cy17:0 to $16:1{\omega}7c$ and cy19:0 to $18:1{\omega}7c$ should be considered as a potential responsible factor for the obvious differentiation that was observed between the biodegradable mulch film soil and PE mulch film soil in a upland field. The results of this experimentation show the potential of using biodegradable mulch film in place of PE.

• Korean Journal of Microbiology
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• v.7 no.3
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• pp.107-114
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• 1969

Relationship of soil properties and seasonal variation on microbilogical population to-continuous culture and first-time culture of ginseng was investigated by bimonthly from May 1976 to January 1977. pH and P contents of 2 years continuous culture of soil were higher than other culture plot of soil, and contraty to the above, 2 years first-time culture of ginseng soil was conplot of soil, and contraty to the above, 2 years first-time culture of ginseng soil was contained more potassium contents than other culture plot of soil. In microbiological fluctuation with seasonr in various soil conditions, the population, trends of Fusarium spp., Erwiniaspp., and flourescent Psedudomonas spp. were increased in May and July in general, but decreased in the other month. It was observed that in all type of soil, Fusarium spp. was distributed in abundance in and on rihizosphere, and decreased the propagules numbers as soil depth increase. The numbers of Erwinia spp. and fluorescent Pseudo-monas spp. were distributed greater in numbers on the surface zone of soil depth decreasing the numbers along the soil layer increase, and also in 2years continuous culture of soil especially, a great numbers of Erwinia spp. and fluorescent Pseudomonas were evenly distributed in surface zone and rhizosphere. Ginseng disease with a high incidence of bacterial disease in continuous culture of 2 and 4 years was seemed to be associated with soil bacteria that was high in numbers of Erwinia spp. and fluorescent Pseudomonas spp. in May and July.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.4
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• pp.63-71
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• 2015

Recently the roof gardening has become very popular and even on the apartment-veranda and on the roofs in high buildings many kinds of vegetables and berries are cultivated. And the demand of the nutritional supplement for the effective plant growth is also increasing. The general urban conditions are to be adapted for plant growth. A different hygroscopic and temperature-conditions after regions, sunshine and wind have strong influences on the plant growth and usually it is not optimal enough. It is because why a nutritional condition in soil for plant growth so important and essential. The usual compound-soil or -fertilizer cannot offer enough quantities of nutrients for plant growth and additional soil conditioner becomes more necessary. There are many kinds of soil conditioners like hydrogel in the market and we studied on Geohumus, Montigel and Geko, which are widely used in Europe and other countries. Water absorption and microbial immobilization with effective microorganisms were tested and compared. The EM solution was identified as bacteria, fungi and azotobacter etc. and they were immobilized at the soil conditioners at first. And the cultivated and immobilized at the soil conditioners EM-solution was added to the plant soil. 1 g of the soil conditioners absorbed ca. 20 g of water. The plant grew 10 cm more, got 3 times more branches and 2 times more fruits in the soil with soil conditioners immobilized with microorganisms. With water addition the plant with both conditions in the soil could stay fresh in comparison to without soil conditioners.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.261-261
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• 2005

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.102-102
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• 1998

No Abstract, See Full Text

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.73.1-73
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• 2002

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.10a
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• pp.66.2-66
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• 1999

• Proceedings of the KSEEG Conference
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• 2007.04a
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• pp.37-39
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• 2007

See Full Text