• Journal of Mushroom
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• v.18 no.4
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• pp.331-338
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• 2020

This study was conducted to cultivate new Lepista nuda varieties with shorter cultivation period and better fruiting body compared to that of wild strains, for mass production and commercial application. Eighteen genetic resources of L. nuda were collected and grown in boxes using rice straw-fermented growth medium. Four lines with fruiting bodies were formed and selected as cross-breeding lines. Although 657 combinations were crossed through monospore crossing, only 17 combinations were bred between the 'CBMLN-19' line and the 'CBMLN-30' line. Among them, 8 lines with fast mycelial growth and high density were selected. After inoculating the rice straw-fermented growth medium with 14 genetic resources and 8 cross-breeding lines, their incubation period was investigated. Six of the cross-breeding lines completed their incubation in 20 days, while 7 of the 14 genetic resources took more than 40 days to complete their incubation, reducing the incubation period by more than 20 days in most cross-breeding lines. After the incubations were completed, the clay loam soil was covered with for post-cultivation, and when the mycelial cultivation was complete, the formation of fruiting bodies was induced after scraping the mycelial bodies under these environmental conditions: 14℃, 95% relative humidity or higher, and 1,500 to 2,000 ppm CO2 concentration. The temperature was reduced to 6℃ at night, resulting in a low temperature shock. Thus, 4 lines of fruiting bodies occurred from two genetic resources 'CBMLN-31' and 'CBMLN-44' and two cross-bred lines 'CBMLN-96' and 'CBMLN-103'. After inoculation, the longest period for fruiting bodies to occur was 100 days for the control:, the genetic resource 'CBMLN-31', and the shortest period (45 days) was observed for the cross-breeding line 'CBMLN-103'. The result of the investigation of the fruiting body characteristics shows that the cross-bred line 'CBMLN-103' showed a small form with 1.9 g of individual weight and 123validstipes per box, which was the highest incidence among the four lines. Another cross-bred line, 'CBMLN-96', had an individual weight of 5.5 g, which is larger than that of 'CBMLN-103'; however, the number of valid stipes per box was 30 less than that of 'CBMLN-103'. Quantity analysis showed that the control, 'CBMLN-31', had the highest quantity of 783 g per box, followed by the cross-bred line, 'CBMLN-96' with 165 g per box, and then the 'CBMLN-103' with 232 g. The quantity of the two crossbred lines was lower than that of the control 'CBMLN-31'; however, the amount of fruiting bodies was higher, and the cultivation period was shortened by 32 to 33 days. Therefore, these two lines would be selected as superior lines.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.4
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• pp.279-288
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• 2021

Low-density transplanting is a cultivation technology that reduces labor and production costs. In this study, the growth and yield of several varieties with different tillering characteristics were analyzed in order to establish an appropriate planting density for low-density transplanting. Varieties with Low-Tillering (LT), Medium-Tillering (MT), and High-Tillering (HT) were planted at a density of 37-80 hills/3.3 m². As the planting density decreased, the number of tillers per hill increased, but the number of tillers per square meter of hill decreased, especially for the LT variety. Decreasing density extended the tillering stage, which was longest in the LT variety. As the planting density decreased, SPAD(Soil plant analysis development, chlorophyll meter) values just before heading increased while canopy light interception decreased. Such changes were much greater in the LT variety than in the MT and HT varieties. The heading date tended to be delayed by 0-2 days as the planting density decreased, and there was no difference in the length of the period from first heading to full heading. As the number of spikelets per panicle increased, the number of spikelets per square meter did not differ according to the planting density. Decreasing planting density did not affect the grain weight; nevertheless, the yield ultimately decreased because of the decreasing ripening rate. The optimal planting density for stable low-density transplanting cultivation was determined to be over 50 hills/3.3 m2. In addition, these results suggest that LT varieties should be avoided, since these showed large decreases in growth and yield with decreasing planting density.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.4
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• pp.251-267
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• 2021

Remotely sensed vegetation indices (VIs) are empirically related with gross primary productivity (GPP) in various spatio-temporal scales. The uncertainties in GPP-VI relationship increase with temporal resolution. Uncertainty also exists in the eddy covariance (EC)-based estimation of GPP, arising from the partitioning of the measured net ecosystem CO2 exchange (NEE) into GPP and ecosystem respiration (RE). For two forests and two agricultural sites, we correlated the EC-derived GPP in various time scales with three different near-surface remotely sensed VIs: (1) normalized difference vegetation index (NDVI), (2) enhanced vegetation index (EVI), and (3) near infrared reflectance from vegetation (NIRv) along with NIRvP (i.e., NIRv multiplied by photosynthetically active radiation, PAR). Among the compared VIs, NIRvP showed highest correlation with half-hourly and monthly GPP at all sites. The NIRvP was used to test the reliability of GPP derived by two different NEE partitioning methods: (1) original KoFlux methods (GPP_Ori) and (2) machine-learning based method (GPP_ANN). GPP_ANN showed higher correlation with NIRvP at half-hourly time scale, but there was no difference at daily time scale. The NIRvP-GPP correlation was lower under clear sky conditions due to co-limitation of GPP by other environmental conditions such as air temperature, vapor pressure deficit and soil moisture. However, under cloudy conditions when photosynthesis is mainly limited by radiation, the use of NIRvP was more promising to test the credibility of NEE partitioning methods. Despite the necessity of further analyses, the results suggest that NIRvP can be used as the proxy of GPP at high temporal-scale. However, for the VIs-based GPP estimation with high temporal resolution to be meaningful, complex systems-based analysis methods (related to systems thinking and self-organization that goes beyond the empirical VIs-GPP relationship) should be developed.

• Journal of Life Science
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• v.32 no.7
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• pp.510-522
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• 2022

In this study, to biosynthesize PHA with properties more similar to polypropylene, a Bacillus sp. EMK-5020 strain that biosynthesized poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was isolated from soil. Bacillus sp. EMK-5020 strain biosynthesized PHBV containing 1.3% 3-hydroxyvalerate (3HV) using reducing sugar contained in Makgeolli lees enzymatic hydrolysate (MLEH) as a single carbon source. As the amount of propionic acid, which was added as a second carbon source, increased, the content of 3HV also increased. PHBV containing up to 48.6% of 3HV was synthesized when 1.0 g/l of propionic acid was added. Based on these results, the strain was cultured for 72 hr in a 3 l fermenter using reducing sugar in MLEH (20 g/l) and propionic acid (1 g/l) as the main and secondary carbon sources, respectively. As a result, 6.4 g/l DCW and 50 wt% of PHBV (MLEH-PHBV) containing 8.9% 3HV were biosynthesized. Through gel permeation chromatography and thermogravimetric analysis, it was confirmed that the average molecular weight and the decomposition temperature of MLEH-PHBV were 152 kDa and 273℃, respectively. In conclusion, the Bacillus sp. EMK-5020 strain could biosynthesize PHBV containing various 3HV fractions when MLEH and propionic acid were used as carbon sources, and PHBV-MLEH containing 8.9% 3HV was confirmed to have higher thermal stability than standard PHBV (8% 3HV).

• Journal of Korean Society of Forest Science
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• v.111 no.2
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• pp.201-223
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• 2022

Currently, species extinctions are increasing due to climate change and continued anthropogenic impact. We selected 300 species for conservation with emphasis on plants co-occurring in the Baekdudaegan area, which is a large ecological axis of Korea. We aimed to investigate the vegetation community and environmental characteristics of Zabelia tyaihyonii in the limestone habitat among the target plant species in the Baekdudaegan region to derive effective conservation strategies. In Danyang-gun, Yeongwol-gun, and Jecheon-si, we selected 36 investigation sites where Z. tyaihyonii was present. We investigated the vegetation, flora, soil and physical environment. We also found notable plants such as Thalictrum petaloideum, Sillaphyton podagraria, and Neillia uekii at the investigation sites. We classified forest vegetation community types into 4 vegetation units and 7 species group types. With canonical correspondence analysis (CCA) of the vegetation community and habitat factors, we determined the overall explanatory power to be 75.2%, and we classified the environmental characteristics of the habitat of Z. tyaihyonii into a grouping of three. Among these, we detected a relationship between the environmental factors elevation, slope, organic matter, rock ratio, pH, potassium, and sodium. We identified numerous rare and endemic plants, including Thalictrum petaloideum, in the investigation site, and determined that these groups needed to be preserved at the habitat level. In the classification of the vegetation units analyzed based on the emerging plants and the CCA, we reaffirmed the uniqueness and specificity of the vegetation community in the habitat of Z. tyaihyonii. We anticipate that our results will be used as scientific evidence for the empirical conservation of the native habitats of Z. tyaihyonii.

• Journal of Life Science
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• v.32 no.11
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• pp.872-881
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• 2022

In this study, we optimized the composition of the indole-3-acetic acid (IAA) production medium using response surface methodology on Pantoea agglomerans SRCM 119864 isolated from soil. IAA-producing P. aglomerans SRCM 119864 was identified by 16S rRNA gene sequencing. There are 11 intermediate components known to affect IAA production, hence the effect of each component on IAA production was investigated using a Plackett-Burman design (PBD). Based on the PBD, sucrose, tryptone, and sodium chloride were selected as the main factors that enhanced the IAA production at optimal L-tryptophan concentration. The predicted maximum IAA production (64.34 mg/l) was obtained for a concentration of sucrose of 13.38 g/l, of tryptone of 18.34 g/l, of sodium chloride of 9.71 g/l, and of L-tryptophan of 6.25 g/l using a the hybrid design experimental model. In the experiment, the nutrient broth medium supplemented with 0.1% L-tryptophan as the basal medium produced 45.24 mg/l of IAA, whereas the optimized medium produced 65.40 mg/l of IAA, resulting in a 44.56% increase in efficiency. It was confirmed that the IAA production of the designed optimal composition medium was very similar to the predicted IAA production. The statistical significance and suitability of the experimental model were verified through analysis of variance (ANOVA). Therefore, in this study, we determined the optimal growth medium concentration for the maximum production of IAA, which can contribute to sustainable agriculture and increase crop yield.

• Journal of the Korean Institute of Landscape Architecture
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• v.51 no.2
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• pp.12-27
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• 2023

In this study, among the construction-type parks in Gangdong-gu, targeting parks with high environmental and ecological value located on the urban green axis, a plan was prepared for the ecological renewal of urban parks, and a design that applied to them was proposed. The renewal target site was selected by analyzing the general condition of Gangdong-gu and urban parks, the land use and green area ratio, park green area, and the green axis of Gangdong-gu. Gangdong-gu has 54 parks, including 2 neighborhood parks and 52 children's parks. In the first stage of the current status review, 17 parks were extracted through locational value analysis, such as location and adjacency to the natural axis and green axis. In the second stage, eight parks were selected among the first-stage extraction parks based on the ratio of green spaces and open spaces within each park service area. In the third stage, two of the second stage extraction parks were selected based on whether the legal standard of the park area was met, and in the fourth stage, one of the third stage extraction parks was selected through an aging survey of the park. As for the urban ecological status of the renewal target site, the status of land use in the aspect of entropy reduction, the status of soil cover in the aspect of water circulation, and the status of planting structure in the aspect of biodiversity were investigated. As for the status of the three renewal sites, the green area was insufficient at 18.3-45.3%, and the facility area was 54.7%-81.7%, which was judged to have low urban temperature reduction effects. The impervious pavement area accounted for 34.5% to 48.9% of the park area, accounting for most of the facility area, and it was judged that the water circulation function was insufficient. The planting structure consisted of a single layer and a double layer structure, and although the tree layer was good, the lower vegetation was poor, and there was no planting site of edible plants or large hardwood trees, so the biodiversity was low. After the ecological renewal design of Seonrin Children's Park, Dangmal Children's Park, and Saemmul Children's Park, which were selected as the renewal targets in this study, the ecological area ratio of each park increased by 1.4 to 3 times than before the renewal. If the urban parks located on the urban green axis are examined from the perspective of the urban ecosystem and renewed ecologically, it is judged that the expected effect will be high in reducing entropy, improving water circulation, and laying the foundation for biodiversity in terms of the urban ecosystem.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.41 no.4
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• pp.40-48
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• 2023

This study is part of a foundational research effort aimed at developing a suitability index for breeding grounds related to avian activities along the domestic South and West coasts, including islands. Focus Group Interviews (FGI) and Analytic Hierarchy Process (AHP) analyses were conducted. The results are as follows. First, as a result of determining the value of the suitability of coastal bird breeding sites, the 'Natural Value(0.763)' was higher than the 'Artificial Value(0.237)'. Other artificial values were identified as sub-ranked except for 'Protected Areas' to ensure continuous integrity of breeding spaces. Second, as a result of re-establishing the 25 evaluation items classified in the two-time FGI as higher concepts, nine natural values and five artificial values were finally selected as a total of 14. Third, the results of the mid-classification evaluation of the importance of the suitability of coastal bird breeding sites were identified in the order of 'Ecological Value(0.392)', 'Topographic Value(0.251)', 'Passive Interference(0.124)', 'Geological Value(0.120)', and 'Active Interference(0.113)'. Fourth, the results of the priority of evaluation items of coastal bird breeding sites were in the order of 'Vegetation Distribution (0.187)', 'Area of Mudflats(0.118)', 'Presence or Absence of Mudflats(0.092)', 'Appearance of Natural Enemies(0.087)', 'Protected Areas(0.08)', 'Island Area (0.069)', 'Over-Breeding devastation(0.064)', 'Soil Composition Ratio(0.056)', 'Distance from Land(0.054)', 'Ocean farm area (0.045)', 'Cultivated land area(0.041)', 'Cultivation behavior(0.038)', 'Angle of the Surface(0.036)', and 'Land Use(0.033)'. It is judged that the weighting result value of the evaluation items derived in this study can be used for priority evaluation focusing on the coastal bird breeding area space. However, it seems that the correlation with the unique habitat suitability of bird individuals needs to be supplemented, and spatial analysis research incorporating species-specific characteristics will be left as a future task.

• Journal of Bio-Environment Control
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• v.32 no.4
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• pp.475-483
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• 2023

This study was conducted to determine the optimal irrigation starting point by analyzing tree growth, physiological responses, fruit quality, and productivity in peach orchards. Seven-year-old 'Kawanakajima Hakuto' peach trees were used in an experimental field (35°49'30.4"N, 127°01'33.2"E) located within the National Institute of Horticultural and Herbal Science located in Wanju-gun, Jeollabuk-do. The irrigation starting point was set with four levels of -20, -40, -60, and -80 kPa from June to September 2022. While there were no significant differences in increase of trunk cross-section area and leaf area among treatments, shoot length and diameter decreased in the -80 kPa and -20 kPa treatments. The photosynthetic rate measured in August was highest for -60 kPa (17.7 μmol·m^-2·s^-1), followed by -40 kPa (15.6 μmol·m^-2·s^-1), -20 kPa (14.5 μmol·m^-2·s^-1) and -80 kPa (14.0 μmol·m^-2·s^-1). SPAD value measured in May and August was lower in the -80 kPa and -20 kPa treatments than in the -60 kPa and -40 kPa treatments. The harvest date reached three days earlier in the -20 kPa treatment compared to other treatments. The fruit weight was highest in the -60 kPa (379.1 g), followed by -40 kPa (344.0 g), -80 kPa (321.0 g) and -20 kPa (274.9 g). Firmness was the lowest in the -20 kPa treatment. The soluble solid content was highest in the -60 kPa treatment (13.3°Bx).The ratio of marketable fruits was highest in the -60 kPa treatment (50.7%) and lowest in the -80 kPa treatment (23.4%). In conclusion, we suggest that setting the irrigation starting point at -60 kPa could improve the fruit quality and yield in peach orchards.

• Korean Journal of Environment and Ecology
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• v.38 no.4
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• pp.375-387
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• 2024

This study investigated the ecological characteristics of Fraxinus chiisanensis Nakai, one of the endemic and rare plant species in Korea, based on its distribution status, characteristics of the growth environment, and species composition. A vegetation survey that analyzed the correlation between species distribution patterns and environmental variables, along with the traits of the emergent plant species, was performed according to the explanation of environmental growth conditions and phytosociological method for the location where F. chiisanensis is found. A total of 19 dominant locations and 9 non-dominant locations of F. chiisanensis were observed in 28 study sites in 12 regions, and a total of 155 taxa were observed. According to the vegetation climate of Korea, the growth environment of the study site where F. chiisanensis is located is characterized as cold and is primarily situated within the northern temperate deciduous broadleaf forest zone. The average elevation was 859m above sea level, with an average rock exposure of 60.4%, soil exposure of 24.7%, and an average slope of 18.7°. The taxa belonging to the top P-NCD(Percentage of Net Contribution Degree) among the emergent species were mostly designated as the taxa emerging in valley vegetation. The correlation analysis of environmental variables revealed that altitude had the strongest correlation, with rock exposure showing the second highest correlation. The ongoing dynamics of the F. chiisanensis forest are anticipated to persist due to the high P-NCD values exhibited by the F. chiisanensis within the shrub and herbaceous layers among the taxa associated with tree species. Most F. chiisanensis habitats are currently situated within protected regions such as national parks, provincial parks, and county parks, where there are relatively minimal human-induced disturbances. However, there is potential for damage in areas not designated as protected, such as forest tending operation sites or new hiking trails. Concerns about declining habitat quality have prompted suggestions for management strategies such as establishing Forest Genetic Resource Reserves in these locations. In addition, follow-up and further research should be conducted to identify possible sites for distribution and establish candidate conservation areas based on various environmental conditions of F. chiisanensis.