• Current Research on Agriculture and Life Sciences
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• v.10
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• pp.49-52
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• 1992

This study was carried out to investigate the possibility of utilization of facultative anaerobic photosynthetic bacteria as a bio-fertilizer. R. capsulata B22 and R. spheroides SM11 were added to the soil and the change of organic martter and nitrogen content of the treated soils was analyzed on the course of time. It is apparent that the content of organic matter and nitrogen was slowly increased by 15-20 days.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.1
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• pp.109-114
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• 2006

For the purpose of oligosaccharide production from alginate, the main component in cell walls of brown algae, the alginate degrading bacteria have been screened from the seaweeds and soil. Among the isolated 69 strains, one strain showing the highest degrading activity was selected and identified as Bacillus licheniformis strain. The adequate sodium alginate concentration for growing the Bacillus licheniformis was $2.0\%$. The effective nitrogen source is nutrient broth $(0.1\%)$, and optimum initial pH, NaCl concentration, temperature and incubation time to produce the alginate degrading enzyme were 7.5, $2\%,\;30{\pm}2^{\circ}C$, and 144 hrs, respectively.

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.10a
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• pp.210-232
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• 1998

Tong-il, the high-yielding rice variety bred on early 1970, effected a turning point in modern rice production in Korea. As rice production reached the highest record yield in 1978 with HYV, Korea achieved self-sufficiency in domestic supply of rice (or the first time in (her own) history, HYV required high input of fertilizers and pesticides for proving its yielding ability and needed new techniques such as early nursery-planting to prevent chilling damage. But, farm economy did not follow the successful achievement of rice production because of increased farming cost and inflation.'Tong-il variety has been replaced by new high-yielding Japonica varieties from 1980 when record-low-temperature during summer months had persisted. Also, the cooked rice of Tong-il variety did not agree with the appetite of Korean people. Though the hectarage of Tong-il rice did reduce, farmers applied the same high-input cultural techniques for new Japonica cultivars as did for Tong-il variety. Heavy application of nitrogen fertilizer contaminated surface and ground water with nitrate ions while phosphorous fertilizer was blamed for algae pollution. Frequent spray of pesticide and herbicide reduced significantly the biotic population in paddy ecosystems including insects and soil microorganisms. The new technologies of the 21st century must be directed to produce safe food, to save natural resources, and to preserve a clean environment for human welfare. We need low-input sustainable farming techniques to provide high-yielding crops and to preserve a healthy ecosystem.

• Journal of Species Research
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• v.8 no.4
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• pp.351-364
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• 2019

Here we describe indigenous prokaryotic species in Korea, a total of 42 bacterial strains affiliated to the class Alphaproteobacteria isolated from various environmental samples: fermented vinegar, sea water, beach sand, fresh water, salt flats, moss, algae, activated sludge, and soil. From the high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to predefined bacterial species. There is no official report that these 42 species included in Alphaproteobacteria in Korea: 15 species of 6 genera in the order Rhodospirillales, 12 species of 10 genera in the order Rhizobiales, 10 species of 8 genera in the order Rhodobacterales, 4 species of 4 genera in the order Sphingomonadales and 1 species of 1 genus in the order Caulobacterales. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

• Mycobiology
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• v.50 no.5
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• pp.326-344
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• 2022

The fungal-like family Saprolegniaceae (Oomycota), also called "water mold," includes mostly aquatic saprophytes as well as notorious aquatic animal pathogens. Most studies on Saprolegniaceae have been biased toward pathogenic species that are important to aquaculture rather than saprotrophic species, despite the latter's crucial roles in carbon cycling of freshwater ecosystems. Few attempts have been made to study the diversity and ecology of Saprolegniaceae; thus, their ecological role is not well-known. During a survey of oomycetes between 2016 and 2021, we investigated the diversity and distribution of culturable Saprolegniaceae species in freshwater ecosystems of Korea. In the present study, members of Saprolegniaceae were isolated and identified at species level based on their cultural, morphological, and molecular phylogenetic analyses. Furthermore, substrate preference and seasonal dynamics for each were examined. Most of the species were previously reported as animal pathogens; however, in the present study, they were often isolated from other freshwater substrates, such as plant debris, algae, water, and soil sediment. The relative abundance of Saprolegniaceae was higher in the cold to cool season than that in the warm to hot season of Korea. This study enhances our understanding of the diversity and ecological attributes of Saprolegniaceae in freshwater ecosystems.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.3
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• pp.165-170
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• 2013

In this study, physico-chemical properties and environmental fate were investigated and ecotoxicity tests using fish, daphnia and algae were conducted for an initial ecological risk assessment of 1,2-Benzisothiazol-3-one. Due to low volatility of the test substance under environmental conditions, it is likely to distributed in soil and water environment. The compound has low adsorption in the soil, with low bioconcentration potential. Acute toxicity results showed that 96 h-$LC_{50}$ for Oryzias laties was 4.7 mg/L (measured) and 48h-$EC_{50}$ for Daphnia magna was 3.3 mg/L (measured). In a growth inhibition test with Pseudokirchneriella subcapitata, 72 h-$EC_{50}$ was 0.456 mg/L (growth rate, nominal) and 0.262 mg/L (yield, nominal). Using the acute toxicity value of algae, predicted no-effect concentration (PNEC) in the aquatic environment was determined to be 2.62 ${\mu}g/L$ using an factor of 100. According to globally harmonized system (GHS), the compound was categorized as aquatic acute 1 for algae, while it was categorized as aquatic acute 2 for fish and daphnia. This screening assessment suggests that the test substance may pose ecological risks in the aquatic environment.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.2
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• pp.111-116
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• 1994

These studies were carried out to investigate the possibility on the agricultural utilization of lake sediment collected in the Daecheong Lake. Several parameters to estimate the degree of maturity of compost by windrow system were tested during a 50 day periods, and the results were as follows : During the composting of Daecheong Lake sediment, C/N ratio decreased gradually from 18.9 at the beginning to 13.1 at the final, while CEC increased from 33.5me/100g to 62.5me/100g. The temperature of lake sediment pile was the highest as $52^{\circ}C$ at 15th day and then decreased slowly until $30^{\circ}C{\sim}40^{\circ}C$ after 50 days. The results may be suggested that Daecheong Lake sediment compost reaches an acceptable level of stability after about 50 days. In order to know the effect of the Lake sediment and its compost on the growth of tomato(Lycopersicum esculentum M.), pot experiments were performed. The appiication of lake sediment(AS) itself, sediment organic fertilizer(SOF), and NPK mixed with sediment organic fertilizer(NSF) was more effective for tomato plant height, fresh and dry weight, and chlorphyll content than those of control treatment. Also, the lake sediment compost improved the physico-chemical properties of soil such as pH, total carbon, total nitrogen, available phosphorous and CEC.

• Journal of Korean Society on Water Environment
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• v.25 no.4
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• pp.589-596
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• 2009

The spectroscopic characteristics and chemical oxygen demand (COD) oxidation efficiencies were investigated for dissolved organic matters (DOM) from diverse sources, which may indirectly affect the concentrations and the quality of DOM found in watersheds. The DOM investigated for this study showed a wide range of the percent distributions of refractory organic matter (R-OM) from 8 to 100%. Relatively high R-OM distributions were observed for the DOM with the source of head water, sediments, paddy soils, field soils, and treated sewage whereas the DOM from livestock waste, reed, weeds, algae, and attached algae exhibited lower R-OM percent distributions. The percent distribution of R-OM had positive correlations with specific UV absorbance (SUVA) and humidification indices (HIX) of DOM. The investigated DOM was classified into four different source groups (i.e., biota, vegetables, soils, sediments) by comparing the synchronous fluorescence spectra. The DOM group from biota source was characterized by a prominent presence of protein-like fluorescence (PLF) whereas fulvic-like fluorescence (FLF) was additionally observed for vegetable-source DOM. FLF became significant for the DOM from both soils and sediments although no PLF was found for soil-derived DOM. A range of COD oxidation efficiency was observed for the various DOM, ranging from 36 to 94% and from 65 to 125% for $COD_{Mn}$ and $COD_{Cr}$, respectively. The results indicate that $COD_{Cr}$ reflects the higher OM concentration than $COD_{Mn}$. However, 95% confidence intervals of the COD oxidation efficiencies were similar for the two types of COD, suggesting that $COD_{Cr}$ may not be the superior OM index to $COD_{Mn}$ in terms of the variability of the oxidation efficiency. No significant correlations were obtained between COD oxidation efficiencies and the spectroscopic characteristics of DOM for this study.

• Korean Journal of Soil Science and Fertilizer
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• v.10 no.1
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• pp.23-28
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• 1977

Acetylene-ethylene($C_2H_2-C_2H_4$) assay was carried out to find the heterotrophic nitrogen-fixing activities and distribution of nitrogen fixers in eleven different soil series which are located in Kyeonggi province. Following are the summaries of this study. 1. Acetylene-reducing activities were higher in Gwanghwal, Mangyeong and Buyong series which are derived from fluviomarine deposite than in Yeongsan, Hamchang and Pyeongtack series which are observed from continental alluvial plain. The lowest activities are observed in Yecheon, Hoegog, and Jisan series which are situated in local valley region. 2. Estimated amouts of $N_2$ per annum fixed by $N_2$-fixing heterotrophs were about 3.2kg in fuluvio marine soils, 2.6kg in continental alluvial plain and 2.0kg/10a in local valley region, respectively. 3. Azotobacter and Beijerinckia were not detected in any of the ten different soil series except in Pyeongtaeg series. However, Clostridia, anaerobic nitrogen fixer, was detected in order of ${\times}10^2$. It is assumed that these population are not enough to contribute to the nitrogen supply by the biological fixation in paddy soil. 4. For the assesment of heterotrophic nitrogen fixation in paddy soil, it must be presumed that aerobes, anaerobes and phototrophs which can grow on nitrogen free media may greatly contribute for the asymbiotic netrogen fixation.

• The Korean Journal of Mycology
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• v.46 no.3
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• pp.213-225
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• 2018

Trichoderma (Hypocreaceae) is one of the most ubiquitous genera worldwide. This genus has an excellent ability to adapt to diverse environments, even under poor nutritional conditions, such as in freshwater. However, little is known about the diversity of Trichoderma species in freshwater environments. In this study, we isolated diverse fungal strains from algae, plant litter, and soil sediment in streams in Korea. The strains were identified based on molecular phylogenetic analyses of internal transcribed spacer (ITS) rDNA and translation elongation factor 1 ($TEF1{\alpha}$) sequences. We also investigated their morphological characteristics by microscopic observation and determination of cultural features on different media. As a result, six Trichoderma species were found in Korea: T. afroharzianum, T. capillare, T. guizhouense, T. paraviridescens, T. reesei, and T. saturnisporum. Interestingly, T. paraviridescens showed both cellulose activity and hypoxia stress tolerance phenotypes, indicating its role as a decomposer in freshwater ecosystems. Our study revealed that freshwater environment could be a good candidate for investigating the species diversity of Trichoderma.