• Korean Journal of Veterinary Research
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• v.56 no.3
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• pp.155-160
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• 2016

This study was conducted to evaluate three different mixed formulations of sodium hyaluronate (SH) and carboxymethyl cellulose (CMC) using a low-humidity air flow-induced rat dry eye model and determine the most suitable mixture. The total thickness of the cornea, corneal epithelial thickness, corneal stroma thickness, damaged corneal epithelium percentage region, thickness of the bulbar conjunctiva epithelium, number of goblet cells, goblet cell occupation percentage region, and damaged bulbar conjunctiva epithelium percentage region were measured by histomorphological evaluation. After 5 h exposure to drying airflow, the thickness of the cornea and conjunctiva was decreased with desquamation of the corneal and conjunctiva epithelium. However, these dry eye symptoms were markedly inhibited by treatment with the reference and test formulations. More favorable effects on decreased thickness were detected in response to the CMC than the SH. However, SH had a greater protective effect against corneal and conjunctiva epithelial damage. The application of a mixture of 0.1% SH and 0.2% CMC showed more favorable effects on the corneal and conjunctival damage and the stabilization of the ocular surface than SH or CMC alone.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.1
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• pp.67-74
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• 2023

In this study, for a natural cosmetics market, we sought to explore alternatives that can replace polyvinyl alcohol (PVA) of peel-off packs. A peel-off type pack was prepared by combining pullulan, a water-soluble polysaccharide, and other polysaccharides (sodium hyaluronate, cellulose gum, hydroxyethyl cellulose, sodium alginate, corn starch), and the pH, viscosity, and stability against temperature of each peel-off type pack were confirmed. The thickness and tensile strength of the manufactured film were measured for comparison with the PVA peel-off type pack, and applicability, drying speed, and removal degree were measured. Among them, the pullulan-sodium hyaluronate peel-off type pack showed excellent film formation ability to replace the peel-off type pack containing PVA with 5.12% thin film thickness and 4.23% high film tensile strength. When applied to actual skin, the degree of spread of the pack, the usability that can be uniformly applied, and the formation and removal strength of the film when removed after drying were also similar to the peel-off type pack containing PVA. Therefore, it was confirmed that the film formed of pullulan-sodium hyaluronate showed enough physical properties to replace the PVA of the peel-off type pack as a natural peel-off type pack.

• Journal of the Korean Applied Science and Technology
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• v.38 no.4
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• pp.1003-1009
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• 2021

A study on the associative properties of sodium hyaluronate (NaHA) and Alkane-bis (dimethylalkylammonium bromide) surfactants in aqueous solution was investigated in relation to the chemical structure of surfactants. As a result of measuring the interfacial tension, a parabolic graph showing the minimum value (c_min) at a specific concentration was shown. Above this minimum concentration the increase in interfacial tension is thought to be related to the formation of aggregates of NaHA chains and dimeric surfactants. The plot of viscosity vs surfactant concentration shows a slight maxium at c_min and a viscosity decrease at high surfactant concentrations. Viscosity nonlinear behavior is related to the size increase due to the complex growth and to the size shrinkage following from the interaction with electrolyte ions and free micelles. The results of surface tension measurements show a broad region of surface tension decrease, indicating the NaHA-surfactant interaction. The increase in surface tension above c_min may be related to the adsorption of clusters, consisting of free NaHA chains and dimeric surfactant. The strong adsorption of surfactant is observed at high concentrations.

• Membrane Journal
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• v.15 no.3
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• pp.213-223
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• 2005

We prepared an anti-adhesion membrane made of sodium hyaluronate/sodium carboxymethylcellulose (HA/CMC) and evaluated its effectiveness for adhesion prevention in a rat model. The anti-adhesion membrane was prepared by lyophilizing HA/CMC solution and cross-linking properly with 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC). In a cecum/abdominal wall abrasion model of Sprague-Dawley rat, cecal serosa and abdominal wall were abraded in $1\times2\;(cm^2)$ with a bone burr after peritoneal midline incision and sutured at 3 points around the injured surface. The denuded cecum was covered with HA/CMC membrane (experimental group), or nothing (control group) and apposed to the abdominal wall. Most of the control group represented 3 or more of adhesion grade at POD 7, 14, 21, and 28, whereas $60\~70\%$ of the experimental group was 2 or less of adhesion grade at 14, 21, and 28. It was similar in the adhesion strength. In a general manner, the adhesion grade and strength showed gradual increasing until POD 14, almost same or a little increasing POD 21, but decreasing POD 28. Also the control group was much higher in adhesion grade, strength, and area than the experimental group. It is expected that the anti-adhesion membrane will have a good clinical result in postoperative adhesion prevention.

• Journal of Korean Foot and Ankle Society
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• v.15 no.1
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• pp.27-31
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• 2011

Purpose: This study evaluated the efficacy of an intra-articular injection of sodium hyaluronate in the treatment of early stage osteoarthritis of the ankle. Materials and Methods: Early stage ankle osteoarthritis (Takakura stage I and II) patients who received hyaluronic acid injection therapy were retrospectively reviewed. Patients underwent intra-articular injections of 2 mL of sodium hyaluronate for 3 consecutive weeks. Clinical evaluation and Visual Analogue Scale (VAS) scoring were performed at every three months after treatment. Results: Total 27 patients were involved and mean age was 55 year old (range 33 to 77 years). There were 13 male and 14 female patients. Mean follow up duration was 13 month and. Pre-intra-articular VAS score was $8.9{\pm}0.7$ and three month follow up score was $3.8{\pm}2.8$. VAS score of last follow up was $3.2{\pm}3.4$. The effect of hyaluronic acid continued about one year when analyzed the VAS score change of the patients followed for more than one year. Patients' satisfaction was "very satisfied" in nine, "satisfied" in 12, "fair" in one, and "not satisfied" in five patients. Overall satisfaction rate was 82%. There were no ankle osteoarthritis stage changes in serial follow up radiograph. Conclusion: Symptomatic relief of signs and symptoms of osteoarthritis of the ankle was achieved by injection of an intra-articular hyaluronate injection. Efficacy of Hyaluronate acid injection persisted more than 1 year in our study. Intra-articular hyaluronate injection to ankle osteoarthritis is safe and effective as knee joint and should be considered as a valid conservative treatment for ankle osteoarthritis.

• The Journal of the Korean dental association
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• v.33 no.7 s.314
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• pp.467-471
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• 1995

• Maxillofacial Plastic and Reconstructive Surgery
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• v.21 no.4
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• pp.388-394
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• 1999

To compare the effect of hyaluronate and dexamethasone on the temporomandibular joint arthrocentesis the author investigated 22 temporomandibular joint disorder(TMD) patients with pain and limitation of mouth opening who visited at the Department of Oral and Maxillofacial Surgery. Chosun Dental Hospital and were made a diagnosis as Wilkes stage III or IV of TMJ internal derangement clinically and radiographically. The two groups consisted of 10 patients with injection of sodium hyaluronate 10mg$(Artz^{(R)})$(hyaluronate group) on the upper joint space of the affected temporomandibular joint 5 times at intervals of a week after arthrocentesis, and 12 patients with injection of dexamethasone$(Oradexon^{(R)})$ at a time(dexamethasone group). Maximum mouth opening, pain value and satisfaction value during mastication were assessed on a visual analog scale before arthrocentesis and after 6 months. Then the within-group and between-group differences were evaluated in the obtained data and the clinical success rate of each group was calculated according to our success criteria. The results were as follows. 1. the mean of maximum mouth opening before arthrocentesis and after 6 months in the hyaluronate group were 24.9mm and 39.0mm respectively, and those before arthrocentesis and after 6 months in the dexamethasone group were 25.7mm and 41.3mm respectively. 2. The mean of pain value on a visual analog scale in the hyaluronate group before arthrocentesis and after 6 months were 6.7 and 1.8 respectively, and those in the dexamethasone group before arthrocentesis and after 6 months were 7.0 and 1.8 respectively. 3. The mean of satisfaction value during mastication on a visual analog scale in the hyaluronate group before arthrocentesis and after 6 months were 2.8 and 7.7 respectively, and those in the dexamethasone group before arthrocentesis and after 6 months were 3.1 and 7.8 respectively. 4. There were statistically significant differences between all measurements before arthrocentesis and after 6 months(P<0.001), but no difference between all measurements in the hyaluronate group and those in the dexamethasone group. 5. The over all success rate of the hyaluronate group and the dexamethasone group were 60.0% and 63.6% respectively. In summary, there was significant difference between the effect of hyaluronate and dexamethasone on the temporomandibular joint arthrocentesis but hyaluronate is better than corticosteroid as the injection drug in consideration of the side effect related with repeated injection.

• Bulletin of the Korean Chemical Society
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• v.27 no.3
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• pp.413-418
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• 2006

Sodium hyaluronate (NaHA), water soluble polymer having ultra-high molecular weight, is characterized by using on-line frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) and multiangle light scattering (MALS). This study demonstrates the capability of power programming FI-AFlFFF for the separation of NaHA and the applicability of FI-AFlFFF with MALS for the characterization of molecular weight distribution and their structural information. Since sample injection and relaxation in FI-AFlFFF are achieved by using hydrodynamic relaxation, separation of high molecular weight polymers can be achieved smoothly without halting the separation flow. Experiments are carried out with the two different NaHA products (a raw NaHA sample and a thermally degraded NaHA product) and molecular weight distribution and conformations in solution are determined. Influence of sample filtration on the change of molecular weight distribution is also discussed.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.26 no.1
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• pp.59-80
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• 2000

본 연구는 dermal equivalent를 이용하여 AHA류(lactic acid, glycolic acid, citric acid 및 malic acid) 및 보습 원료(sodium hyaluronate, glycerine, natural carbohydrate complex)에 대한 wound healing 효과 및 그 기작을 알아보기 위한 것이다. AHA류 및 보습 원료의 독성은 dermal equivalent를 이용하여 m assay를 실시한 후 적정 농도를 petri dish위에 조성한 dermal equivalent에 처리하였다. 그리고 wound healing 효과를 알아보기 위하여 collagen 수축률을 측정하였다. 또한 collagen 합성을 촉진하는 것으로 잘알려진 vitamin C도 함께 처리하였다. AHA류의 경우 평균 pH 2-3으로 매우 낮기 때문에 4N NaOH를 이용하여 pH를 6-7사이로 조절한 sample를 함께 실험하였다. 보습 원료의 경우 전반적으로 10% 이상의 높은 NR$_{50}$보여 주었다. 특히 sodium hyaluronate(1% stock solution)의 경우에는 16% NR$_{50}$를 보여 주었다. pH를 6-7로 조절한 AHA류의 경우에는 전반적으로 0.6% 내외의 NR$_{50}$를 보이는 가운데 lactic acid는 상대적으로 높은2% NR$_{50}$를 보여 주었다. Collagen 수축률 측정 실험 결과에서는 2% sodium hyaluronate(1% stock solution)가 대조군에 비하여 처리 후 2일째 25%내외의 향상된 수축률을 보여 주었다. pH를6-7로 조절한 AHA류 중 0.1%의 malic acid의 경우에서는 대조군에 비하여 처리 후 1일째 및 2일째 각각 28% 및 35%의 수축률을 보여 주었으며 pH를 6-7로 조절한 0.1% vitamin C에서도 유사한 결과를 보여 주었다. 반면에 pH를 6-7로 조절한 0.1% citric acid의 경우에는 10-20%의 낮은 수축률을 보여 주었다. MTT assay를 이용한 UV 조사 후 pH를 6-7로 조절한 AHA류의 repairing UV damage 효과에 대한 실험에서 0.1% 및 0.01%의 malic acid와 0.01% citric acid은 irradiation control에 비하여 약 10% 이상 세포수를 증가시켰다. 그러나 예외적으로 citric acid의 경우 0.1% 농도에서 오히려 20%내외로 세포수가 감소되는 경향을 보여주었다. 그리고 lactic acid 및 glycolic acid는 두드러지는 효과를 나타내지 않았다. Collagen 합성을 측정 실험에서는 pH를 6-7로 조절한 AHA류에서는 대조군에 비하여 상대적으로 12-19% 더 합성을 촉진하였다. 반면에 pH가 2-3인 AHA류의 경우에는 대조 군과 유사하거나 조금 낮은 합성율을 보여 주었다.

• Journal of Korean Foot and Ankle Society
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• v.21 no.4
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• pp.151-155
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• 2017

Purpose: Reducing tenderness or pain on the ankle joint and improving the range of motion are thought to be possible using hyaluronate-based anti-adhesive agents. On the other hand, there are more aspects to be studied regarding the incidence of complications, such as resting pain, tenderness, and stiffness, after surgery. Therefore, the aim of this study was to prove the effectiveness of the agents after ankle fracture surgery. Materials and Methods: Patients, who underwent open reduction and internal fixation surgery due to ankle joint fractures from June 2015 to May 2016, were studied prospectively. Thirty patients of them received a $Guardix^{(R)}$ injection during their surgeries and were included in the injection group. The other 30 patients were included in the control group. Postoperatively, tenderness on the scar, a delay in wound healing, and the active range of motion were evaluated at 2, 6, and 12 weeks after surgery. Results: A significant difference in tenderness on the scar was observed 2 weeks after surgery. On the other hand, there was no significant difference at 6 and 12 weeks after the surgery. The agent-using group showed a 6.7% delay in wound healing and a 93.3% nondelaying. In the non-using group, the delay was 63.3%, while non-delay was 36.7% (p<0.001). The group that underwent $Guardix^{(R)}$ usage showed an effective result in the visual analogue scale, which was statistically significant (p<0.001). The result at 6 and 12 weeks after surgery showed a significant difference. Conclusion: Improvement was observed in the patients who underwent a $Guardix^{(R)}$ injection, regarding the range of motion, visual analog scale, and healing of the wound postoperatively.