• BMB Reports
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• 제41권2호
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• pp.164-169
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• 2008

The tumor suppressor gene p53 regulates apoptotic cell death and the cell cycle. In this study, we investigated the role of p53 in nitric oxide (NO)-induced apoptosis in vascular smooth muscle cells (VSMCs). We found that the NO donor S-nitroso-N-acetyl-penicillamine (SNAP) increased apoptotic cell death in p53-deficient VSMCs compared with wild-type cells. The heme oxygen-ase (HO) inhibitor tin protoporphyrin IX reduced the resistance of wild-type VSMCs to SNAP-induced cell death. SNAP promoted HO-1 expression in both cell types. HO-2 protein was increased only in wild-type VSMCs following SNAP treatment; however, similar levels of HO-2 mRNA were detected in both cell types. SNAP significantly increased the levels of non-heme-iron and dinitrosyl iron-sulfur clusters in wild-type VSMCs compared with p53-deficient VSMCs. Moreover, pretreatment with FeSO4 and the carbon monoxide donor CORM-2, but not biliverdin, significantly protected p53-deficient cells from SNAP-induced cell death compared with normal cells. These results suggest that wild-type VSMCs are more resistant to NO-mediated apoptosis than p53-deficient VSMCs through p53-dependent up-regulation of HO-2.

• The Korean Journal of Physiology and Pharmacology
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• 제5권1호
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• pp.87-92
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• 2001

Carbon monoxide (CO) binds to soluble guanylate cyclase to lead its activation and elicits smooth muscle relaxation. The vascular tissues have a high capacity to produce CO, since heme oxygenase-2 (HO-2) is constitutively expressed in endothelial and smooth muscle cells, and HO-1 can be greatly up-regulated by oxidative stress. Moreover, the substrate of HO, heme, is readily available for catalysis in vascular tissue. Although the activation of heme oxygenase pathway under various stress conditions may provide a defence mechanism in compromised tissues, the specific role of HO-1-derived CO in the control of aortic contractility still remains to be elucidated. The present study was done to determine the effect of HO-1 induction on the aortic contractility. Thus, the effects of incubation of aortic tissue with S-nitroso-N-acetylpenicillamine (SNAP) for 1 hr on the aortic contractile response to phenylephrine were studied. The preincubation with SNAP resulted in depression of the vasoconstrictor response to phenylephrine. This effect was restored by HO inhibitor or methylene blue but not by NOS inhibitor. The attenuation of vascular reactivity by preincubation with SNAP was also revealed in endothelium-free rings. $AlF4^--evoked$ contraction in control did not differ from that in SNP-treated group. These results suggest that increased production of CO was responsible for the reduction of the contractile response to phenylephrine in aortic ring preincubated with SNAP and this effect of SNAP was independent on endothelium.

• 디자인학연구
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• 제20권3호
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• pp.289-298
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• 2007

지체가 부자유한 사용자가 컴퓨터를 이용하여 정보를 쉽게 입력하거나, 쉽게 인지할 수 있도록 지원하는 커뮤니케이션 수단으로서 주목받고 있는 것이 EGI(Eye Gaze Interface)시스템이다. 그러나 EGI는 "주시점 탈(脫) 현상(목표 주시점으로부터 실제의 주시점이 벗어나는 현상)" 의 발생으로 인하여 목표정보를 입력하거나 인지하는데 많은 심리적, 생리적 부담을 가중시키는 것이 현실이다. 본 연구는 EGI의 문제점으로서 지적되고 있는 "주시점 탈(脫) 현상" 의 해결방안에 초점을 두고, 누구든지 간단히 조작할 수 있는 인터페이스를 제안, 고찰하였다. "주시점 탈(脫) 현상" 의 원인규명 및 제안하는 인터페이스의 유효성 검증을 위하여, 실험 툴(흡착효과를 부여한 한국어 입력인터페이스)을 제작, 평가하였으며, 심리적 평가(주관평가)와 생리적 평가(뇌파측정)를 실시하여, 흡착효과를 적용했던 경우가 흡착효과를 적용하지 않았던 경우 보다 문자입력이 쉽고 생리적 부담이 적을 수 있다는 가능성을 발견할 수 있었다. 본 연구는 이러한 결과를 통하여, 선천적이든 후천적이든 물리적 핸디캡으로 인하여 커뮤니케이션에 장애를 가지고 있는 사용자들에게도 원활한 커뮤니케이션을 할 수 있는 시스템 개발 지원 가능성에 길을 열었다고 생각하며, 그 효용성에 대해서도 시사하는 바가 있음을 발견할 수 있었다.

• 대한치과보철학회지
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• 제39권6호
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• pp.709-734
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• 2001

The aims of this experiment were to investigate the strain and temperature changes simultaneously within autopolymerzing acrylic resin specimens. A computerized data acquisition system with an electrical resistance strain gauge and a thermocouple was used over time periods up to 180 minutes. The overall strain kinetics, the effects of stress relaxation and additional heat supply during the polymerization were evaluated. Stone mold replicas with an inner butt-joint rectangular cavity ($40.0{\times}25.0mm$, 5.0mm in depth) were duplicated from a brass master mold. A strain gauge (AE-11-S50N-120-EC, CAS Inc., Korea) and a thermocouple were installed within the cavity, which had been connected to a personal computer and a precision signal conditioning amplifier (DA1600 Dynamic Strain Amplifier, CAS Inc., Korea) so that real-time recordings of both polymerization-induced strain and temperature changes were performed. After each of fresh resin mixture was poured into the mold replica, data recording was done up to 180 minutes with three-second interval. Each of two poly(methyl methacrylate) products (Duralay, Vertex) and a vinyl ethyl methacrylate product (Snap) was examined repeatedly ten times. Additionally, removal procedures were done after 15, 30 and 60 minutes from the start of mixing to evaluate the effect of stress relaxation after deflasking. Six specimens for each of nine conditions were examined. After removal from the mold, the specimen continued bench-curing up to 180 minutes. Using a waterbath (Hanau Junior Curing Unit, Model No.76-0, Teledyne Hanau, New York, U.S.A.) with its temperature control maintained at $50^{\circ}C$, heat-soaking procedures with two different durations (15 and 45 minutes) were done to evaluate the effect of additional heat supply on the strain and temperature changes within the specimen during the polymerization. Five specimens for each of six conditions were examined. Within the parameters of this study the following results were drawn: 1. The mean shrinkage strains reached $-3095{\mu}{\epsilon},\;-1796{\mu}{\epsilon}$ and $-2959{\mu}{\epsilon}$ for Duralay, Snap and Vertex, respectively. The mean maximum temperature rise reached $56.7^{\circ}C,\;41.3^{\circ}C$ and $56.1^{\circ}C$ for Duralay, Snap, and Vertex, respectively. A vinyl ethyl methacrylate product (Snap) showed significantly less polymerization shrinkage strain (p<0.01) and significantly lower maximum temperature rise (p<0.01) than the other two poly(methyl methacrylate) products (Duralay, Vertex). 2. Mean maximum shrinkage rate for each resin was calculated to $-31.8{\mu}{\epsilon}/sec,\;-15.9{\mu}{\epsilon}/sec$ and $-31.8{\mu}{\epsilon}/sec$ for Duralay, Snap and Vertex, respectively. Snap showed significantly lower maximum shrinkage rate than Duralay and Vertex (p<0.01). 3. From the second experiment, some expansion was observed immediately after removal of specimen from the mold, and the amount of expansion increased as the removal time was delayed. For each removal time, Snap showed significantly less strain changes than the other two poly(methyl methacrylate) products (p<0.05). 4. During the external heat supply for the resins, higher maximum temperature rises were found. Meanwhile, the maximum shrinkage rates were not different from those of room temperature polymerizations. 5. From the third experiment, the external heat supply for the resins during polymerization could temporarily decrease or even reverse shrinkage strains of each material. But, shrinkage re-occurred in the linear nature after completion of heat supply. 6. Linear thermal expansion coefficients obtained from the end of heat supply continuing for an additional 5 minutes, showed that Snap exhibited significantly lower values than the other two poly(methyl methacrylate) products (p<0.01). Moreover, little difference was found between the mean linear thermal expansion coefficients obtained from two different heating durations (p>0.05).

• 패션비즈니스
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• 제9권1호
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• pp.79-88
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• 2005

The purpose of this study is to propose the design of the patient's clothes for a disabled child between 4 and 6. For this, in this study, I would present the following design plan which includes 3 upper garments, 2 trousers and a one-piece dress. In case of the damage on head and neck, a neck line should be deep and wide thereby providing ease to the clothes. To facilitate injection and dressing/undressing, there should be parting from neck to cuff which can be fixed by snap or button. By rolling up sleeves with strings inside the cuffs and fixating plaster cast with snap button outside the cuffs. In case of body cast with plaster cast around body part, front adjusting part should be wider and the width should be adjusted with strings. In case of hip spica cast which covers waist, I separated front part and rear part, fixated them with snap buttons, gave more width to front adjusting part with strings to adjust width, which constitute one-piece dress. In case of shoulder spica cast, the other shoulder which is not covered with plaster cast should be exposed, and to prevent clothes' coming down, strings with snap button or velcro should be attached over a shoulder so that the length of the strings can be adjusted. In case of applying plaster cast or aid to the whole part of a leg, one part of trousers should be shorter so as to expose the injured part and there should be a parting with strings or velcros on the side for easy dressing/undressing. When plaster cast or splint is short, The strings are meant to adjust length of trousers. The partings are located 2 cm from side lines toward the center.

• Current Research on Agriculture and Life Sciences
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• 제22권
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• pp.1-12
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• 2004

Pod-edible bean or snap bean is a fairly new crop to domestic farmers but the national demand is steadily increasing in recent years along with the development of western food business and change in dietary patterns. At the same time, much efforts are being made to export it to foreign country, mainly to Japan. The amount of seeds introduced from outside is also continuously increasing along with the enlargement of area planted for the crop. Hybridization breeding for the crop has already been started to supply the cheaper and better seeds which will reduce the seed costs and foster the higher income to the farmers. In this experiment, several technologies related with the production of quality seeds are preliminary investigated. Some of the results obtained are summarized as follows; 1. Highly significant interaction was recognized between planting dates and no. of pods per plant and no. of branches but no interaction between planting dates and plant height and no. of nodes on main stem. Days to maturity was proportionally reduced to later planting dates. 2. Rate of viviparous pods and seeds was gradually increased in later planting dates but rate of germination was increased in earlier planting dates with lower germination rate in white seed coat grains than in colored seed ones. 3. Seed yield was higher in the earlier planting dates with a great deal of varietal difference. Early to mid April was considered to he the optimum planting dates for snap bean in Kyungbuk area. High correlation was recognized between seed yield and no. of pods per plant, no. of seeds per plant, and 100 seed weight. 4. Days to flowering was three and seven days longer in Cheongsong, high mountainous area than in Kunwi, somewhat prairie lowland. One hundred seed weight was also higher in Cheongsong than in Kunwi. Rate of viviparous grains, pods, and decayed seeds was higher in Cheongsong but, at the same time, the rate of germination and seed yield was also higher in Cheongsong. 5. One hundred seed weight of KLG5007 increased continuously up to 35days after flowering and decreased thereafter but that of KLG50027 increased to 40days after flowering and slowly reduced thereafter. The content of crude oil reached to maximum at 40 days after flowering and reduced thereafter. The rate of germination in Gangnangkong 1 was the highest, 89.3%, at 35 days after flowering and reduced thereafter while that in KLG50027 reached to maximum, 70.7%. at 40days after flowering and reduced thereafter. Thus, the optimum harvesting time for snap bean was considered to be 35~40days after flowering. 6. The snap bean pods at yellow bean stage easily became viviparous ones under saturated moisture conditions for 24 hours at $25{\sim}30^{\circ}C$. Therefore, it is recommended to harvest pods somewhat earlier than yellow-bean stage and let them do post maturing, especially when it is to be rained.

• 한국식품영양과학회지
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• 제33권8호
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• pp.1367-1372
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• 2004

보리의 도정과정중에 생성되는 도정부산물의 식품소재로서의 활용 방안으로 보리 도정 겨를 밀가루에 0∼30% 대체한 쿠키와 머핀을 제조하여 품질 특성을 조사하였다. 보리겨의 첨가는 쿠키의 퍼짐성, 밝기, 관능특성을 감소시키는 것으로 나타났다. 그러나 20% 첨가수준까지 보리 겨를 첨가하는 것은 쿠키의 top grain, 색, 풍미, 텍스쳐 그리고 전반적인 기호도에서 양호한 점수를 얻었으며 특히 10% 첨가수준에서는 관능특성에서 대조구와 유의적으로 큰 차이가 없는 것으로 나타났다. 머핀의 경우에도 보리 도정 겨의 첨가는 부피를 감소시키고 내부 기공의 특성이 떨어지는 결과를 초래하였지만 20% 첨가 수준 범위내에서 색, 풍미, 텍스쳐 등 머핀의 품질에 크게 부정적이지 않는 것으로 평가되었다.

• 대한두개안면성형외과학회지
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• 제20권6호
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• pp.361-369
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• 2019

Background: Trauma is one of the most common causes of enophthalmos, and post-traumatic enophthalmos primarily results from an increased volume of the bony orbit. We achieved good long-term results by simultaneously using an anatomical absorbable implant and iliac bone graft to correct post-traumatic enophthalmos. Methods: From January 2012 to December 2016, we performed operations on seven patients with post-traumatic enophthalmos. In all seven cases, reduction surgery for the initial trauma was performed at our hospital. Hertel exophthalmometry, clinical photography, three-dimensional computed tomography (3D-CT), and orbital volume measurements using software to calculate the specific volume captured on 3D-CT (ITK-SNAP, Insight Toolkit-SNAP) were performed preoperatively and postoperatively. Results: Patients were evaluated based on exophthalmometry, clinical photographs, 3D-CT, and orbital volume measured by the ITK-SNAP program at 5 days and 1 year postoperatively, and all factors improved significantly compared with the preoperative baseline. Complications such as hematoma or extraocular muscle limitation were absent, and the corrected orbital volume was well maintained at the 1-year follow-up visit. Conclusion: We present a method to correct enophthalmos by reconstructing the orbital wall using an anatomical absorbable implant and a simultaneous autologous iliac bone graft. All cases showed satisfactory results for enophthalmos correction. We suggest this method as a good option for the correction of post-traumatic enophthalmos.

• 한국조리학회지
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• 제17권1호
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• pp.171-183
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• 2011

본 연구는 홍삼분말을 쿠키에 첨가하여 품질특성에 미치는 영향 및 제조적성을 알아보고자 하였다. 일반 성분은 우리 밀보다 홍삼분말이 낮은 결과를 보였으며 우리 밀에 홍삼분말을 첨가한 경우 수분은 유의적 차이가 없었고 WRC, AWRC는 유의적으로 증가하였다. 제조 및 품질 특성은 두께와 퍼짐성은 유의적 차이가 없었고 직경은 대조구가 가장 크며 우리 밀 홍삼 분말 쿠키는 차이가 없었다. L, b 값은 유의적으로 감소하는 경향을 보였고, a값은 유의적으로 증가하였다. 즉, 홍삼분말 첨가 시 외부색상은 첨가량 증가에 따라 어두워지는 경향을 보였다. 관능 특성은 부피, crust, top grain, 모든 부분에서 대조구가 가장 좋게 나타났고 부피는 6% 첨가구가 가장 좋은 결과를 보였으며 첨가 구간 유의적 차이는 없었다. 전체적으로 대조구와 우리 밀에 홍삼분말을 첨가한 경우 12%까지 제품으로 최적 첨가량으로 보아진다.

• 대한두개안면성형외과학회지
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• 제10권1호
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• pp.19-22
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• 2009

Purpose: There are various approaches for open reduction surgery for orbitozygomatic fractures. After the incisions are performed, patients might have various complications. In this point of view, we conducted a retrospective study of the usability of infraorbital incision on elderly patients, generally more vulnerable to scleral show and ectropion. Methods: In this study, 20 patients who are relatively more vulnerable to scleral show and ectropion through snap-back test having infraorbital incision were reviewed. We monitored the duration of the surgery, and complications resulted from the incision. We evaluated the scars with the researcher's observations and with the patient's level of satisfaction on a scale of 1 to 5. Results: On average, 5 minutes was spent between the skin incision and the fracture exposure. No case of scleral show and ectropion occurred. One year follow-up observation revealed 4 cases of visible scars, 9 cases of barely visible scars, and 7 cases of invisible scars. The subjective satisfaction level of the patients' scar is an average of 4.0. Conclusion: Infraorbital incision has many advantages. First, it can reduce the complications such as scleral show and ectropion. Second, it can shorten the operation time. Third, it can diminish incision-related scars.