• The Journal of Internal Korean Medicine
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• v.11 no.1
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• pp.77-91
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• 1990

This study was carried out to investigate the effect of Jasoeumja extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows: 1. The isolated trachea smooth muscle was suspended in the Organ bath with oxygenated Kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with Isometric transducer (Nacro F-60). The resting tension was approximately 0.5g. 2. The trachea smooth muscle of the isolated guinea pig was significantly relaxed by the administration of Jasoeumja extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine $10^{-4}M$ was significantly inhibited by Jasoeumja extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine $10^{-4}M$ was considerably inhibited by Jasoeumja extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to 5-hydroxytryptamine $10^{-4}M$ was considerably inhibited by Jasoeumja extract.

• The Journal of Internal Korean Medicine
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• v.11 no.2
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• pp.68-79
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• 1990

This study was carried out to investigate the effect of Heapyoyangjintang and Haepyoejintang extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows : 1. The isolated trachea smooth muscle was suspended in the organ bath with oxygenated kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with Isometric transducer(nacro F-60). The resting tension was approximately 0.5g. 2. The trachea smooth muscle of the isolated guinea pig was significantly relaxed by the administration of Haepyoyangjintang and Haepyoejintang extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine$10^4M$ Was significantly inhibited by Heapyoyangjintang and Haepyoejintang extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine$10^4M$ was considerably inhibited by Haepyoyangjintang and Haepyoejintang extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to 5-hydorxytrtrypptamine $10^4M$ was considerably inhibited by Haepyoyangjintang and haepyoejintang extract.

• The Journal of Internal Korean Medicine
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• v.13 no.2
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• pp.84-94
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• 1992

This study was carried out to investigate the effect of samyootang and shinchulsan extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate it's mechanism The isolated were obtained as follows ; 1. The isolated trachea smooth muscle was suspended in the organ bath with oxygenated kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recored with Isometric transducer (nacro F-60) The resting tension was approximately 0.5g 2. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine $10^{-4}M$ was significantly inhibited by samyootang and shinchulsan extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine $10^{-4}M$ was considerably inhibited by samyootang and shinchulsan extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine $10^{-4}M$ was significantly inhibited by samyootang and shinchulsan extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine $10^{-4}M$ was significantly inhibited by samyootang and shinchulsan extract.

• The Journal of Dong Guk Oriental Medicine
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• v.4
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• pp.211-236
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• 1995

This study was carried out to investigate the effects of Mahwangtang and Gamimahwangtang extract and its constituent herbs on the contractile force of rat tracheal smooth muscle treated with acetylcholine and to elucidate its mechanism. The results of this study were follows ; 1. Mahwangtang and Gamimahwangtang significantly inhibited the contractile response of isolated rat tracheal smooth muscle by acetylcholine, and Gamimahwangtang more significantly effects than Mahwangtang. 2. Gamimahwangtang without Ephedrae Herba significantly inhibited the contractile response of isolated rat tracheal smooth muscle by acetylcholine and less inhibited the contractile force than Gamimahwangtang. 3. Gamimahwangtang without Fritillariae Roylei Bulbus, Platycodi Radix, Cinnamomi Ramulus, significantly inhibited the contractile response of isolated rat tracheal smooth muscle by acetylcholine and less inhibited the contractile force than Gamimahwangtang. 4. Gamimahwangtang without Armeniacae Amarum Semen, Glycyrrhizae Radix significantly inhibited the contractile response of isolated rat tracheal smooth muscle by acetylcholine and its difference did not to the Gamimahwangtang. 5. Gamimahwangtang without Ginseng Radix significantly inhibited the contractile response of isolated rat tracheal smooth muscle by acetylcholine and more significantly effects than Gamimahwangtang.

• The Korean Journal of Physiology
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• v.23 no.1
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• pp.99-108
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• 1989

The effects of prostaglandin $(PGF_{2{\alpha}})$ on the contractility of vascular smooth muscle were investigated in the helical strip of the rabbit aorta. The aortic strip was immersed in the phosphate-buffered Tyrode's solution which was equilibrated with 100% $O_{2}$ at $35^{\circ}C$ and its isometric tension was measured. The contraction was induced by $(PGF_{2{\alpha}})$, norepinephrine (NE), or potassium (40 mM) in the nomal Tyrode's solution (1 mM, $Ca^{2+}$) or $Ca^{2+}-free$ Tyrode's solution. Effects of verapamil and phentolamine on the contraction were also observed. The aortic strip began to contract at the concentration of $5\;{\mu}g%$ and reached the maximal contraction at the concentration of $150\;{\mu}g%$ $(PGF_{2{\alpha}})$. The maximal contraction was corresponded respectively to $52.2{\pm}3.0%$ and $81.5{\pm}3.5%$ of maximal contraction by NE $(1{\times}10^{-5}M)$ and 40 mM $K^{+}$. And the maximal contractions by $(PGF_{2{\alpha}})$ or NE were induced at the concentration of about 1 mM $Ca^{2+}$. $(PGF_{2{\alpha}})$ induced the contraction of aortic strip even after induction of contraction by 40 mM $K^{+}$ and the contraction by $(PGF_{2{\alpha}})$ was not blocked by the ${\alpha}-receptor$ blocker, phentolamine. And the contraction by the $(PGF_{2{\alpha}})$ was inhibited partially by a verapamil at the concentration of $1{\times}10^{-5}M$ and the contraction began to increase at the concentration of $1{\times}10^{-4}M$ verapamil. Whereas the contraction by NE was completely blocked by verapamil. Though both the $(PGF_{2{\alpha}})$ and NE induced the contraction in the $Ca^{2+}-free$ Tyrode's solution, the peak tension was not maintained. But the rate of tension decline was lower in the contraction by $(PGF_{2{\alpha}})$ than in that by NE. The verapamil did not inhibit the contraction by $(PGF_{2{\alpha}})$ in the $Ca^{2+}-free$ Tyrode's solution and increased the contraction at the concentration of above $1{\times}10^{-4}M$. The NE-induced contraction in the $Ca^{2+}-free$ Tyrode's solution was inhibited completely by a verapamil. From the above results it is suggested that the contraction induced by $(PGF_{2{\alpha}})$ results from the promotion of the both $Ca^{2+}$ influx and the intracellular $Ca^{2+}$ release by different way from NE.

• The Journal of Korean Physical Therapy
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• v.15 no.4
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• pp.442-454
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• 2003

The aim of this study was to demonstrate the effects of silver spike point (SSP) low frequency electrical stimulation on plasma vasoactive intestinal polypeptide (VIP) activities measured by radioimmunoassay from volunteer and the effects of VIP on pain substance-induced contraction investigated by isometric tension methode in animal. The current of 3 Hz continue type, but not 100 Hz continue type, of SSP low frequency electrical stimulation significantly increased in plasma VIP from normal volunteer. The pain substance, such as norepinephrine, serotonin, and prostaglandin $F2{\alpha}$, increased vascular smooth muscle contraction, respectively. These responses were inhibited by VIP applied cumulatively (1 nM - $1\;{\mu}M$), but not serotonin-induced contraction. In addition, serotonin, and prostaglandin $F2{\alpha}$ induced uterine smooth muscle contraction from rat. However, these responses were inhibited by VIP ($1\;{\mu}M$), only serotonin-induced contraction. These results suggest that the VIP regulates pain substance in part and that the SSP low frequency electrical stimulation, specifically current of 3 Hz continue type, significantly increases plasma VIP from volunteer.

• The Korean Journal of Physiology
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• v.29 no.1
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• pp.29-37
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• 1995

Gastric smooth muscle of guinea pigs was used to investigate whether the inhibitory effect of calcium antagonists on tonic contraction was accompanied by inhibition of phospholipase C activity. Tonic contraction and $[^{3}H]$ inositol phosphate (IP) formation in response to acetylcholine were measured after pretreatment with verapamil, nifedipine, 8-(N,N-diethylamino)octyl 3,4,5-trimethoxy-benzoate (TMB-8) or EGTA. Verapamil $(10\;{\mu}M)$, TMB-8 $(10\;{\mu}M)$ or EGTA (2 mM) significantly inhibited acetylcholine $(1\;{\mu}M)$-stimulated tonic contraction but nifedipine (100 nM) did not. Acetylcholine dose-dependently increased the formation of $[^{3}H]IP$. This effect was not observed in the presence of 2 mM EGTA. Both verapamil and TMB-8 significantly inhibited $[^{3}H]IP$ formation induced by $10\;{\mu}M$ acetylcholine, whereas nifedipine did not. In a subsequent study, we measured phospholipase C activity in gastric muscle cell homogenate and in permeabilized cells to determine whether calcium antagonists could inhibit the activity directly. The calcium antagonists did not change the phospholipase C activity of the cell homogenate or the permeabilized cells. But EGTA decreased phospholipase C activity by 50%. These results suggest that the inhibitory effects of verapamil and TMB-8 on acetylcholine-stimulated tonic contraction may be accompanied by inhibition of phospholipase C activity.

• Journal of Ginseng Research
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• v.20 no.2
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• pp.133-138
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• 1996

Rabbit corpus cavernosal smooth muscle strips submaximally precontracted with phenylephrine (5$\times$$10^6$ M) and treated with increasing concentrations of Korean ginseng (1, 5, 10, 20, 30, 40 mg/ml) showed tension decrease concentration-dependently (1 mg/ml: 1.7%, 5 mg/ml: 10.2%, 10 mg/ml: 22.7%, 20 mg/ml: 44.0%, 30 mg/ml: 65.2%, 40 mg/ml: 95.6%). Relaxations to Korean ginseng were inhibited significantly by endothelial disruption, by pretreatment with methylene blue, pyrogallol, L-NNA and atropine. Pretreatment of the muscle strips with ginseng caused concentration-related inhibition of a phenylephrine induced contraction, and in calcium-free high potassium depolarizing solution, decreased basal tension as well as inhibited contraction induced by Caclr. Korean ginseng also produced the reduction of responses to depolarizing medium(50, 40, 60 mM KCI). With these results we can confirm the relaxation effect of ginseng at a dose dependent on the cavernosal smooth muscle and suggest that its action is mediated by multiple action mechanisms that include increasing the release of nitric oxide from the corporal sinusoids. Increasing intracellular calcium sequestration, and a hyperpolarizating action.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.5
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• pp.547-553
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• 1997

It has been known that activation of tyrosine kinases is involved in signal transduction. Role of the tyrosine kinase in vascular smooth muscle contraction was examined in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Male Sprague-Dawley rats underwent uninephrectomy, one week after which they were subcutaneously implanted with DOCA (200 mg/kg) and supplied with 1% NaCl and 0.2% KCl drinking water for $4{\sim}6$ weeks. Control rats were treated the same except for that no DOCA was implanted. Helical strips of carotid arteries were mounted in organ baths for measurement of isometric force development. Genistein was used as a tyrosine kinase inhibitor. Concentration-response curves to 5-hydroxytryptamine (5-HT) shifted to the right by genistein in both DOCA-salt hypertensive and control rats. Although the sensitivity to genistein was similar between the two groups, the maximum force generation by 5-HT was less inhibited by genistein in arteries from DOCA-salt hypertensive rats than in those from controls. Genistein-induced relaxations were attenuated in arteries from DOCA-salt rats. Genistein affected the contraction to phorbol 12, 13-dibutyrate (PDBu) neither in DOCA-salt nor in control arteries. These observations suggest that tyrosine kinase is involved in 5-HT-induced vascular contraction, of which role is reduced in DOCA-salt hypertension.

• Korean Journal of Veterinary Research
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• v.35 no.2
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• pp.279-285
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• 1995

To characterize non-adrenergic non-cholinergic(NANC) nerve mediated contractile responses in circular smooth muscle of bovine reticular groove, we investigated NANC relaxation and contraction induced by electric field stimulation to enteric nerves. In the presence of atropine($1{\mu}M$) and guanethidine($50{\mu}M$), electric field stimulation at frequency of 1 to 16Hz(square pulses, 0.5ms duration, 70V) evoked clear-cut relaxations through stimulations. Transient 'rebound contraction' was occured when the stimulus was switched off. All of the responses (relaxation and rebound contraction) were dose-dependently blocked by Nw-nitro-$_{\small{L}}$-arginine methyl ester(L-NAME), an inhibitor of nitric oxide synthesis, and methylene blue, and inhibitor of soluble guanylate cyclase. Tetraethyl ammonium(TEA), a potassium channel blocker, did not block the NANC relaxations.