• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.189-189
• /
• 2022

Soil salinity negatively affects plant growth, productivity, and metabolism. Rice is known to have more sensitive phenotypes than other cereal crops, such as wheat, sorghum, and barley. We characterized the molecular function of rice C3HC4 as a really interesting new gene (RING). Oryza sativa RING finger protein H2-3 (OsRFPH2-3) was highly expressed in 100 mM NaCl. To identify the localization of OsRFPH2-3, we fused vectors that include C-terminal GFP protein (35S;;OsRFPH2-3-GFP). OsRFPH2-3 was expressed in the nucleus in rice protoplasts. An in vitro ubiquitin assay demonstrated that OsRFPH2-3 possessed E3-ubiquitin ligase activity. However, the mutated OsRFPH2-3 were not possessed any E3-ubiquitin ligase activity. Under normal conditions, there is no significant phenotypic difference between transgenic plants and WT plants. However, OsRFPH2-3-overexpressing plants exhibited higher fresh weight and length under saline conditions. Also, transgenic plants maintain higher chlorophyll, proline, and soluble sugar contents and lower H₂O₂ and MDA contents than the wild type; these results support transgenic plants with enhanced salinity tolerance phenotypes.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2023.04a
• /
• pp.156-156
• /
• 2023

Salinity is a major abiotic stress that limits rice productivity in many regions of the world. In order to develop salt stress tolerant rice plants, genetic engineering is a promising approach. We characterized the molecular function of rice C3H2C3 as a really interesting new gene (RING). Oryza sativa RING finger protein H2-3 (OsRFPH2-3) was highly expressed in 100 mM NaCl. To identify the localization of OsRFPH2-3, we fused vectors that include C-terminal GFP protein (35S;;OsRFPH2-3-GFP). OsRFPH2-3 was expressed in the nucleus in rice protoplasts. An in vitro ubiquitin assay demonstrated that OsRFPH2-3 possessed E3-ubiquitin ligase activity. However, the mutated OsRFPH2-3 were not possessed any E3-ubiquitin ligase activity. Under salinity conditions, OsRFPH2-3-overexpressing plants exhibited higher chlorophyll, proline, SOD, POD, CAT, and soluble sugar contents and lower H₂O₂ accumulation than wild-type plants, supporting transgenic plants with enhanced salinity tolerance phenotypes. OsRFPH2-3-overexpressing plants exhibited low Na+ accumulation and Na+/K+ ratios in their roots. Theses results suggest that overexpression of OsRFPH2-3 can make plant insensitivity about salinity conditions.

• Journal of Digital Contents Society
• /
• v.15 no.3
• /
• pp.381-386
• /
• 2014

Multi-touch technology with the development of IT technology, various forms of recognition technology is positioned as the technology is one. A computer and a human action virtualization technology needed to design a natural interface while haneundedo influence. Human-computer interaction possible through intuitive to many users being able to reach without having to learn the sensory capabilities, will be useful if you could use. This paper presents the design of interfaces to the smart table environment to a conventional multi-touch touch-based, real so that you can use your finger to all the purposes. Growing day by day through the ubiquitous electronic blackboard, desk, furniture and electronics industry is actively being made by IT to incorporate multi-touch based interface because the design is expected to be important. Not far in the future, new ways of learning environment by combining modernization and smart table for meeting various IoT not only affect the environment looks anticipating.

• The Journal of the Korea Contents Association
• /
• v.17 no.11
• /
• pp.609-620
• /
• 2017

This study set out to examine the effects of usage characteristics of web novels, which have recently gained popularity in the Internet and mobile platforms, on the satisfaction and continued usage intention of users. For that purpose, the investigator conducted a survey with 333 web novel users. The survey results show that web novel users recorded a high level of flow when they had a challenging spirit and proficiency in the process of using the content. Their high level of flow in web novels had positive impacts on their satisfaction and continued usage intention for web novels. When they were highly satisfied with web novels, they intended to use the content continuously. Those findings indicate that web novel users should be encouraged to have a challenging spirit and proficiency in the content consumption process so that they can develop flow in the content. In addition, it is suggested that there should be efforts to increase their flow and satisfaction to promote their continued usage of web novels.

• Journal of Korea Multimedia Society
• /
• v.15 no.11
• /
• pp.1384-1390
• /
• 2012

It is well known that the exercising and stimulating of fingers have an important bearing on the brain. We take note of the fact and develope a game device for improving one's health and brain ability in respect of the education and training. Especially, we develope the device focused on the balanced exercising of five finger for improving brain function. The game device is possible to used in two-ways, namely online and off-line mode. In online mode, the device is connected with other visual devices such as smart phone and smart TV and communicated with Bluetooth and it is used as a MMI(multi-modal interface) device. Whereas, in off-line mode the game device works independently and it makes possible to enjoy auditorial and tactual games without video images for promotion of brain activity and emotional cultivation. For certification of the device, we implement two games(a fishing game for off-line mode and a shooting game for online mode) for people of all age, especially good for the elderly. It is usable as a game device to offering the elderly a great help for preventing impairment of the cognitive functions.

• Journal of Digital Contents Society
• /
• v.19 no.8
• /
• pp.1433-1442
• /
• 2018

This study develops and improves the EZ Touch App of the scorecard application (app) using the smartphone and the pad, and works with the customer management system (C/S). The research was conducted as follows. First, how do you handle the EZ Touch input method on a scorecard? Second, how to configure the platform of customer (member) management system (C/S) and data server system? Third, does EZ Touch App work organically with customer management system (C/S)? The developed EZ Touch is entered into the scorecard as an input method using the gesture as a result of this research, and it is linked with the C/S system to organize the review function, hall information function, field coaching function through score, It can be used for applications such as information management functions and statistics through differentiated statistics. However, there are some problems and improvements in user convenience in real time use. I think there is a need to study to solve this problem in the future. EZ Touch input method is input to the scorecard by inputting gesture of the finger as a result of this study, and it is linked with this, and it is possible to use differentiated statistics such as review function, hall information function, field coaching function, It is the purpose of the study to improve the technical competitiveness of the product by developing the application.