• 제목/요약/키워드: Small and large intestine

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Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis

  • Wang, Jin;Fan, Huan;Han, Ye;Zhao, Jinzhao;Zhou, Zhijiang
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권1호
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    • pp.100-110
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    • 2017
  • Objective: The gastrointestinal tract of sheep contain complex microbial communities that influence numerous aspects of the sheep's health and development. The objective of this study was to analyze the composition and diversity of the microbiota in the gastrointestinal tract sections (rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum) of sheep. Methods: This analysis was performed by 454 pyrosequencing using the V3-V6 region of the 16S rRNA genes. Samples were collected from five healthy, small tailed Han sheep aged 10 months, obtained at market. The bacterial composition of sheep gastrointestinal microbiota was investigated at the phylum, class, order, family, genus, and species levels. Results: The dominant bacterial phyla in the entire gastrointestinal sections were Firmicutes, Bacteroidetes, and Proteobacteria. In the stomach, the three most dominant genera in the sheep were Prevotella, unclassified Lachnospiraceae, and Butyrivibrio. In the small intestine, the three most dominant genera in the sheep were Escherichia, unclassified Lachnospiraceae, and Ruminococcus. In the large intestine, the three most dominant genera in the sheep were Ruminococcus, unclassified Ruminococcaceae, and Prevotella. R. flavefaciens, B. fibrisolvens, and S. ruminantium were three most dominant species in the sheep gastrointestinal tract. Principal Coordinates Analysis showed that the microbial communities from each gastrointestinal section could be separated into three groups according to similarity of community composition: stomach (rumen, reticulum, omasum, and abomasum), small intestine (duodenum, jejunum, and ileum), and large intestine (cecum, colon, and rectum). Conclusion: This is the first study to characterize the entire gastrointestinal microbiota in sheep by use of 16S rRNA gene amplicon pyrosequencing, expanding our knowledge of the gastrointestinal bacterial community of sheep.

황기건중탕합리중탕(黃耆建中湯合理中湯)이 항궤양(抗潰瘍) 및 위장관(胃腸管) 기능(機能)에 미치는 효과(效果)에 대(對)한 실험적(實驗的) 연구(硏究) (An experimental study of Wahngkigunchungtanghapleechungtang on the effects of gastric ulcer and gastro-intestinal function in rats and mice)

  • 성은미;백태현
    • 대한한방내과학회지
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    • 제18권1호
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    • pp.242-254
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    • 1997
  • An experimental studies were done to investigate the spontaneous movements to the isolated ileum with the liquid extracts of Wahngkigunchunghapleechungtang. Then the effects of isolated ileum, pylorus-ligated and indomethacin-induced ulcer, gastric juice secretion, and transport abilities of the small and large intestine were studied. The following results have been obtained; 1. Wahngkigunchunghapleechungtang showed to have an inhibitory effects on the smooth muscle contraction of the isolated ileum by acetylcholine chloride and barium chloride. 2. Wahngkigunchunghapleechungtang showed to have an inhibitory effect on the contraction of the gastric fundus-strip by acetylcholine chloride and barium chloride. 3. The preventive effects were not recognized on the pylorus-ligated ulcer, by administration of Wahngkigunchunghapleechungtang. 4. The preventive effects were recognized on the indomethacin-induced ulcer by administration of Wahngkigunchunghapleechungtang. 5. The Inhibitory effects on the secretion gastric juice, free & total acidity, and pepsin output were recognized by administration of Wahngkigunchunghapleechungtang. 6. The transport abilities in the small intestine were not recognized by administration of Wahngkigunchunghapleechungtang. 7. The transport abilities in the large intestine were increased by administration of Wahngkigunchunghapleechungtang. According to the above results, it is considered that the experimental effects of Wahngkigunchunghapleechungtang has correspond to the oriental medical literatures.

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교통사고(交通事故)로 인한 편타성(鞭打性) 손상(損傷) 환자(患者) 37례(例)에 대한 임상연구(臨床硏究) (The clinical study on 37 cases of whiplash injury patients which caused by traffic accident)

  • 강재희;장석근;이현;이병렬
    • Journal of Acupuncture Research
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    • 제19권3호
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    • pp.180-191
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    • 2002
  • Objective : This study was performed to evaluate the effect of oriental medical treatment in whiplash injury patients which caused by traffic accident. Methods : The clinical study was carried out 37 cases of whiplash injury patients which had been admitted in Daejon university Cheon-an oriental hospital from June, 2001 to December, 2001. Results : l. In the distribution of sex distinction was female much more than male in the ratio of 3:2 and thirties was most in age distribution. 2. In situation of traffic accident, The most case was rear-ending(70.27%). 3. Except cervical pain or it's reffered pain, there were whole body symptoms as fallow, insomnia in 11cases(29.73%), night pain in 10 cases(27.03%), general body pain in 8cases(21.62), dizziness in 6cases(16.22%). 4. According to Meridian Muscle theory, a injured parts of patient were devided as follows. There were 22cases(59.46%) which have injury in Bladder, Small Intestine Meridian Muscle, 10cases(27.03%) in Gallblader, Triple Energizer Meridian Muscle, 5cases(13.51%) in Stomach, Large Intestine Meridian Muscle. 5. There were 6cases(16.22%) of the grade of complete recovery, 10cases(27.03%) of the grade of excellent, 12cases(32.43%) of the grade of improvement, 6cases(16.22%) of the grade of disimprovement and 3cases(8.11%) of the grade of poor. So 28cases(75.68%) were efficacious. 6. Generally Herb-meds that have efficacy of Geo-Eohyeol were most used(27cases, 72.97%) in early stage. Secondly Herb-med that have efficacy of Yiqi-sunqi were used(20case, 54,05%) in middle stage and Herb-med of Bo-Qiheol were used(l0cases, 27.03%) in latter term. The most used Herb-med was Hoisu-san, Oyaksungi-san(22cases, 54.96%). 7. In l5cases which have Aqua-Acupuncture treatment, There were 3cases of Complete recovery, 5cases of Excellent, 4cases of Improvement, 2cases of Disimprovement, 1cases of Poor. 8cases(53.33%) were above Excellent grade, so it is more efficatious Than simple treatment without Aqua-Acupuncture(36.37%). 8. The improvement of Bladder, Small Intestine Meridian Muscle was 77.27%, Gallblader, Triple Energizer Meridian Muscle was 70% and Stomach, Large Intestine Meridian Muscle was 80%. Additional Aqua-Acupuncture treatment improved to be effective in Bladder, Small Intestine Meridian Muscle(77.78%), in Gallblader, Triple Energizer Meridian Muscle(75%), in Stomach, Large Intestine Meridian Muscle(100%). Conclusions : In this study, Oriental treatment especially Aqua-Acupuncture was effective in whiplash injury patients which caused by traffic accident.

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EAV의 측정치(測定値)와 병증유형(病症類型)의 상관성(相關性)에 관(關)한 연구(硏究) (A syudy on the correlativity of EAV (Electroacupuncure acc.Voll)'s measurement and symptoms of a disease)

  • 한주석;송일병
    • 대한한방내과학회지
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    • 제15권2호
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    • pp.383-417
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    • 1994
  • By making use of the EAV(Electroacupuncture acc. Voll) combined meridian theory of oriental medicine with electronics which was contrived to recognize the physiological and pathological changes of human body, the following conclusions were made in comparison with EAV measurements and types of symptoms(anxiety & headache, fatigue, palpitation, dizzness, abdominal distension. nausea, gastric disturbance. constipation & diarrhea, fatty liver, cva), QSCC, and blood type test. 367 patients including 124 with nervous gastrointestinal problems were selected for this research. 1. From the point of variance of the tested patients 124 nervous gastrointestinal patients, Liver meridian and Spleen meridian showed hyperenergia and Large intestine meridian, Circulation meridian, Triple warmer meridian showed hypoergia 2. In each symptom as the nervous gastrointestinal symptom Liver meridian showed hyperenergia, Large intestine meridian, Circulation meridian and Triple warmer meridian showed hypoergia . 3. In an objective comparison with other symptoms, firstly among the headache & anxiety group left Gall Bladder, Triple warmer and Stomach meridian showed remarkable hypoergia, secondly among fatigue group showed hypoergia in Triple warmer meidian and hyperenergia of Stomach meridian. and thirdly among palpitation group showed hypoergia of Kidney meridian, and lastly among dizzness group showed hypoergia of Gall Bladder, Stomach, Circulation and Small intestine meridian. 4. All of gastric disturbance, nausea, abdominal distention, constipation and diarrhea group showed hyperenergia in Stomach meridian and Spleen meridian. gastric disturbance group showed remarkably hypoergia in Circulation. Small intestine, Lung and Large intestine meridian. Nausea group showed hypoergia in Gall bladder and Urinary bladder meridian. Abdominal distenton group showed hypoergia of Large intestine. Constipation and diarrhea group showed hypoergia of Kidney and left Circulation meridian. 5. Fatty liver group showed hyperenergia of Liver meridian of 83.3%, Gall Bladder, Stomach and Spleen meridian. Urinary bladder and Kidney meridian showed hypoergia 6. CVA group showed hyperenergia in Liver and Circulation meridian. 7. Blood type in typical classification had no significant bearings on each other. 8. QSCC for the attempt of objective materials of constitutional diagnosis had no correlativity in comparison with EAV measurements. In conclusion EAV is thought be used as a diagnostic method in oriental medicine and further research is needed regarding it can be used as a useful method for verifying the characteristics and early finding of symptoms.

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EAV의 측정치(測定値)와 병증유형(病症類型)의 상관성(相關性)에 관(關)한 연구(硏究) (A study on the correlativity of EAV (Electroacupuncure acc.Voll)'s measurement and symptoms of a disease)

  • 한주석;송일병
    • 사상체질의학회지
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    • 제7권1호
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    • pp.43-67
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    • 1995
  • By making use of the EVA(Electroacupuncture acc. Voll) combined meridian theory of oriental medicine with electronics which was contrived to recognize the physiological and pathological changes of human body, the following conclusions were made in comparison with EAV measurements and types of symptoms(anxiety & headache, fatigue, palpitation, dizzness, abdominal distension, nausea, gastric distubance, constipation & diarrhea, fatty liver, CVA), QSCC, and blood type test. 367 patients including 124 with nervous gastrointestinal problems were selected for this research. 1. From the point of variance of the tested patients 124 nervous gastrointestinal patients, Liver meridian and Spleen meridian showed Hyperenergia and Large intestine meridian, Circulation meridian, Tripe warmer meridian showed hypoergia. 2. In each symptom as the nervous gastrointestinal symptom Liver Meridian showed hyperenergia, Large intestine meridian, Circulation meridian and Triple warmer meridian showed hypoergia. 3. In an objective Comparison with other symptoms, firstly among the headache & anxiety group left Gall Bladder, Triple warmer and Stomach meridian showed remarkable hypoergia, secondly among fatigue group showed hypoergia in Triple warmer meridian and hyperenergia of Stomach meridian, and thirdly among palpitation group showed hypoergia of Kidney meridian, and lastly among dizzness group showed hypoergia of Gall bladder, Stomach, Circulation and Small intestine meridian. 4. All of gastric distubance, nausea, abdominal distention, constipation and diarrhea group showed hyperenergia in Stomach meridian and spleen meridian, gastric disturbance group showed remarkably hypoergia in Circulation, Small intestine, Lung and Large intestine meridian, Nausea group showed hypoergia of large intestine, Constipation and diarrhea group showed hypoergia of Kidney and left Circulation meridian. 5. Fatty liver group showed hyperenergia of Liver meridian of 83.3%, Gall Bladder, stomach and Spleen meridian, Urinary bladder and Kidney meridian showed hypoergia. 6. CVA group showed hyperenergia in Liver and Corculation meridian. 7. Blood type in typical classification had on signigicant bearings on each other. 8. QSCC for the attempt of objective materials of constitutional diagnosis had no correlaticity in comparison with EAV measurements. In conclusion EAV is thought be used as a diagnostic method in oriental medicine and further research is needed regarding it can be used as useful method for verifying the characteristics and early finding of symptoms.

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개의 소화관점막 재생에 관한 외과적 연구 (An Experimental Study on the Mucosal Epithelization of the Digestive Tract in the Dog)

  • 장경진
    • 대한수의학회지
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    • 제14권1호
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    • pp.107-113
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    • 1974
  • This study was performed for the purpose of investigating the possibility of epithelization on the defect of digestive tract after covering it with serosal wall of the cecum in the dog. The results obtained were as fallows: 1. Each defect of the digestive tract revealed almost complete formation of the mucous membrane and the muscle. Especially it was most fast in the jejunum. 2. Fistula formation was not found in all the experimental cases. The defects of small intestine and large intestine near the cecum were healed more fastly than the other portion. 3. The stomach and the duodenum appeared intestinal adhetion more frequently than the jejunum and the colon. Therefore, it is considered that covering with serosal wall of the cecum in the lower intestine is far better than in the upper intestine.

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실장산(實腸散)이 위장관(胃腸管)에 미치는 영향(影響) (Experimental studies on the efficacy of Siljangsan)

  • 공현식;유봉하;박동원;유기원
    • 대한한방내과학회지
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    • 제15권2호
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    • pp.197-208
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    • 1994
  • In order to investigate the efficacy of Siljangsan by using the experimental animals, the action of gastric juice secretion, antiulcer. transport rates in the intestine and anti-cathartic action were studied. The following results were obtained. 1. Preventive effects on ulcer induced by pylorus-ligated and Ethanol-HCI were recognized 2. Suppression effects on gastric juice secretinon, free of acidity and total acidity were recognized, but pepsin output was not recognized. 3. For the transport ability of intestine of mice, the ability of small intestine was activated and large intestine was suppressed in the group administrated 4. Anti-cathatic action of mice induced by castor oil was recognized. According to above results, effect based on oriental medical reference was consistent with the actual experimental effect.

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가족성 산종성 대장용종증이 동반된 bronchial carcinoid: 1례 (Bronchial Carcinoid with Familial Adenomatous Polyposis Coli - 1 Case)

  • 김용환
    • Journal of Chest Surgery
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    • 제25권5호
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    • pp.544-549
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    • 1992
  • Carcinoid tumors can be ubiquitous, but most will originate in four sites: appendix [38%], small intestine[24%], rectum[13%] or bronchus[11.6%]. And bronchial carcinoids are rare, accounting for only 1% to 6% of all primary lung tumors. Familial adenomatous polyposis cali, the most common form of the polyposis syndromes attributable to a genetic defect, is defined by demonstration of at least 100 adenomatous polyps in the large intestine. We experienced a case of typical bronchial carcinoid with familial adenomatous polyposis cali in 23 year old female patient, which was surgically treated by left lower lobectomy.

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무플론 요네병 발생 사례 (Paratuberculosis in mouflon (ovis musimon) : A case report)

  • 배유찬;김하영;김희진;윤순식;박중원;진영화;조경오;강문일
    • 대한수의학회지
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    • 제46권3호
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    • pp.271-274
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    • 2006
  • A 2-years-old female domesticated mouflon with a clinical history of chronic diarrhea and emaciation was submitted to NVRQS. Grossly, there were severe thickening of small intestine wall and enlargement of mesenteric lymph nodes. Microscopically, severe granulomatous inflammation was found in small and large intestine, mesenteric lymph nodes, spleen and liver. By Ziehl-Neelsen stain, innumerable acid-fast rod bacteria were found in the cytoplasm of epitheloid and Langhans type giant cells present in these organs. By PCR assay with primer pair specific for Mycobacterium avium subspecies paratuberculosis(IS 900) with small intestine sample, strong positive reaction was detected, although the organism was not isolated from this organ. Based on the results of histopathology and PCR, we concluded that the case was a typical paratuberculosis in mouflon. As far as we know, this is the first case report of paratuberculosis in mouflon Korea.

인영촌구비교맥진(人迎寸口比較脈診)에 관한 연구 (A Study on Comparative Pulse Diagnosis of Renying Pulse(人迎脈) and Cunkou Pulse(寸口脈))

  • 윤창열
    • 대한한의학원전학회지
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    • 제32권4호
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    • pp.35-46
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    • 2019
  • Objectives : While Comparative Pulse Diagnosis of Renying pulse(人迎脈) and Cunkou pulse(寸口脈) is one of the three major pulse diagnostic methods in "Huangdineijing" along with Three Positions and Nine Indicators Pulse Diagnosis(三部九候脈診法) and Cunkou Pulse Diagnosis(寸口脈診法), it has died out in later periods. This study aims to examine this lost method. Methods : Annotations of "Huangdineijing" were examined along with descriptions of the author's own experience. Results & Conclusions : Renying is the Renying(人迎) point from the Stomach Channel(ST), while Cunkou is the Taiyuan(太淵) point from the Lung Channel(LU). These two points are compared in order to determine the deficiency and excess of the Zangfu(臟腑). Normal pulses(平脈) are Soft(軟脈) or Moderate(緩脈), while Stirred pulses(躁脈) are Stringy(弦脈), Tight(緊脈), Slippery(滑脈) or Long(長脈). If the Renying is once active where Shaoyang pulse is active, purge the Gallbladder and supplement the Liver. If there is Stirred pulse, purge the Triple Burner and supplement the Pericardium. If the Renying is twice active where Taiyang pulse is active, purge the Bladder and supplement the Kidney. If there is Stirred pulse, purge the Small Intestine and supplement the Heart. If the Renying is three times active, where Yangming pulse is active, purge the Stomach and supplement the Spleen. If there is Stirred pulse, purge the Large Intestine and supplement the Lung. If the Cunkou is once active where the Jueyin pulse is active, purge the Liver and supplement the Gallbladder. If there is Stirred pulse, purge the Pericardium and supplement the Triple Energizer. If the Cunkou is twice active where the Shaoyin pulse is active, purge the Kidney and supplement the Bladder. If there is stirred pulse, purge the Heart and supplement the Small Intestine. If the Cunkou is three times active where the Taiyin pulse is active, purge the Stomach and supplement the Spleen. If there is Stirred pulse, purge the Lung and supplement the Large Intestine.