• Animal cells and systems
• /
• 제15권4호
• /
• pp.268-273
• /
• 2011

Transgenic mice expressing green fluorescent protein (GFP) under the control of human glial fibrillary acidic protein promoter (hGFAP) have been utilized for in vivo labeling of astrocytes. Although it has been considered that virtually all astrocytes express GFP in this transgenic mouse, we found that different subsets of GFAP-expressing astrocytes express considerably different levels of GFP in the adult brain. Astrocytes in the spinal cord, the molecular layer of thecerebellum, meninges, white matter, corpus callosum and blood vessels exhibited strong GFP, whereas subsets of astrocytes associated with granule cells in the cerebellum and dentate gyrus did not or only marginally exhibited GFP. We also found that a small subset of GFP-expressing cells in the periglomeruli of the olfactory bulb did not express GFAP immunoreactivity. Collectively, these results suggest that human GFAP promoter-derived GFP expression does not faithfully recapitulate the endogenous GFAP expression in mice, suggesting that upstream regulatory mechanisms controlling GFAP transcription are different in different populations of astrocytes, and may reflect the functional diversity of astrocytes.

• 해양환경안전학회지
• /
• 제23권3호
• /
• pp.301-312
• /
• 2017

CE RCD (Recreational Craft Directive) is a certification for the design and construction of small vessels, including pleasure yachts, which are widely used not only in the countries within the European Union, but also in Japan and Southeast Asia. Recently, South Korean leisure craft shipyards have developed interest in exporting to foreign leisure craft markets such as Europe; however, they have encountered difficulties because of the CE RCD regulations, which are relatively complex and difficult to understand. The requirements for buoyancy and stability, which are essential properties that must be understood within the early stage of ship design, are defined based on ISO 12217. However, preparing this assessment according to ship classification regulations is an exceedingly complex task, even with knowledge of naval architecture. In this research, we have developed design support tools to systematically support assessments and preemptively define design information so that buoyancy and stability assessments based on ISO 12217 can be systematically prepared. Our research results were applied to actual examples of yacht design to confirm validity. We believe that the improved yacht design process presented in this research can act as a foundational reference for enhancing the effectiveness and systematic buoyancy and stability assessments.

• Tuberculosis and Respiratory Diseases
• /
• 제38권4호
• /
• pp.389-395
• /
• 1991

Pulmonary hemorrhage is a feature of several immune and idiopathic disorders. The specific etiology of this disease is determined by clinical, serological and renal pathology. Microscopic polyarteritis nodosa is a vasculitis of small vessels which is characterized by involvement of the lung, kidney and skin in young individuals. Its clinical manifestations differ from classical polyarteritis nodosa. It should be considered in differentiation of recurrent alveolar hemorrhage and hematuria. We reported a case of microscopic polyarteritis nodosa which was confirmed by renal biopsy and positive serum antineutrophil cytoplasmic antibody, associated with recurrent alveolar hemorrhage and hematuria.

• 비파괴검사학회지
• /
• 제8권1호
• /
• pp.12-21
• /
• 1988

The behaviour of pressure vessel made of SS41 steel was investigated during hydrotest. AE tests were carried out for the vessels in as-manufactured, V-notched and weld-cracked state using microcomputer-based AE instrumentation. The following results were obtained: 1) In the case of source location using cylindrical program, to minimize the error of source location, the difference of max. Delta T values measured from each sensor should be kept as small as possible. 2) When crack grew, AE event rate increased continuously but AE event from the inclusions occurred intermittently, so by analyzing event rate, the source of AE could be derived. 3) From the spot welding part of supporter, many events with low energy occurred independent of Kaiser effect, which could be confirmed by analyzing energy parameter. 4) The b-value from the tensile specimen of was lower than that from normal specimen and the b-value from crack propagation was lower than that from deformation, so by analyzing peak amplitude distribution, the source mechanism could be derived from the b-value.

• 한국소음진동공학회논문집
• /
• 제25권12호
• /
• pp.815-821
• /
• 2015

Maritime safety has been more critical recently due to the occurrence of shipboard accidents involving prime movers. As such, the propulsion shafting design and construction plays a vital role in the safe operation of the vessel other than focusing on being cost-efficient. Smaller vessels propulsion shafting system normally install high speed four-stroke diesel engine with reduction gear for propulsion efficiency. Due to higher cylinder combustion pressures, flexible couplings are employed to reduce the increased vibratory torque. In this paper, an actual vibration measurement and theoretical analysis was carried out on a propulsion shafting with V18.3L engine installed on small car-ferry and revealed higher torsional vibration. Hence, a rubber-block type flexible coupling was installed to attenuate the transmitted vibratory torque. Considering the flexible coupling application factor, reduction gear stability due to torque variation was analyzed in accordance with IACS(International Association of Classification Societies) M56 and the results are presented herein.

• 대한수의학회지
• /
• 제37권4호
• /
• pp.817-823
• /
• 1997

Suckling piglets and weaned pigs showed anorexia, dehydration, severe abdominal breathing, emaciation and paresis from Oct. 1993. to Nov. 1993. Five 2-week-old piglets were submitted for diagnosis in Kangwon National University. At necropsy, the pin-point well demarcated yellowish white foci were scattered on the surface of the lung, heart, liver, spleen and kidney. Histologically, multifocal areas of necrosis with mononuclear cells infiltration were found in the lung, heart, liver, lymph node, spleen, kidney and small intestine. These lesions tended to be associated with blood vessels. Variable round to ovoid tachyzoites were located at the periphery of the lesions. The organisms were demonstrated as Toxoplasma gondii by immunohistochemical staining method. Ultrastructurally, this parasite was surrounded with parasitophorous vacuole in alveolar macrophage. The parasite was crescent-shaped and $6{\sim}8{\times}1{\sim}2{\mu}m$ in size. It was enclosed by an thick outer membrane and an underlying thin inner membrane. Several club-shaped paired organelles and conoids lay in the cytoplasm at the anterior. Numerous round body and one to several mitochondria were presented in the cytoplasm. Based on the gross findings, histopathology, immunohistochemical and electron microscopic findings, this case was diagnosed as toxoplasmosis in piglets.

• 대한수의학회지
• /
• 제48권4호
• /
• pp.463-467
• /
• 2008

Granulomatous meningoencephalomyelitis (GME) is a sporadic, idiopathic, non suppurative inflammatory disease of the canine central nervous system. GME appears to have a worldwide distribution and to occur mostly in young to middle-age dogs of small breeds. A 6-year-old female mixed dog with wry neck, ataxia and rolling was submitted to the Cheju National University for diagnosis. Grossly, in the brain, cerebrospinal fluid was mildly increased and dilatation of the subarachnoid blood vessels was observed. Histopathologically, the lesions were characterized by perivascular cuffs of lymphocytes, various numbers of macrophages and plasma cells in the brainstem and cerebral white matter. Numerous granuloma composed of lymphocytes and histiocytes were scattered throughout the brainstem. Two malacic foci characterized by axonal swelling and gitter cell infiltration with hemorrhage were noted in the medulla oblongata and cerebellum. Special stains failed to demonstrate any infectious agents. Immunohistochemically, the infiltrated cells demonstrated strong positive reactions for CD3, a marker for T lymphocytes origin. Based on the clinical signs, histopathology, and immunohistochemistry, this case was diagnosed as GME in a mixed dog.

• Journal of Chest Surgery
• /
• 제21권5호
• /
• pp.877-881
• /
• 1988

A coronary arteriovenous fistula represents an abnormal communication from a coronary artery that may enter any cardiac chamber, a pulmonary artery, the coronary sinus, the superior vena cava or the pulmonary vein. We had a successful experience with 46 year-old male who complained exertional dyspnea[NYHA classification II] and anginal pain since 5 years ago. In intensive study of cardiac catheterization and coronary cineangiography, multiple bilateral coronary arteriovenous fistulas and mitral stenoinsufficiency with left atrial thrombi were recognized. The coronary arterio-venous fistula of left coronary artery was revealed large tortuous aberrant vessels that were connected between just distal portion of first diagonal branch of left anterior descending artery and main pulmonary artery. Other fistula was small tortuous vessel which was originated from left atrial branch of left circumflex artery, was drained into left atrium. The fistula of right coronary artery was communicated conal branch of right coronary artery to main pulmonary artery. But there was no 0y step-up in the right cardiac catheterization. The operative procedure were suture-ligation of draining orifice of coronary arteriovenous fistula in main pulmonary artery, mitral valve replacement[Ionescu-Shiley 25mm] with removal of left atrial thrombi and plication of left atrium under the extracorporeal circulation. The postoperative course was uneventful without any complication and discharged without problem at 17th postoperative days.

• Archives of Reconstructive Microsurgery
• /
• 제16권1호
• /
• pp.14-22
• /
• 2007

Perforator free flap (PFF) is currently a major reconstructive option for soft tissue reconstruction in adults. Although PFFs have been used commonly, most reconstructive surgeons still hesitate to perform PFFs in children. The main cause of concern is the perceived high failure rate related to the small diameter of children's perforator vessels. We present 8 consecutive cases of successful transfer of thoracodorsal artery (TDA) & anterolateral thigh (ALT) perforator flap in children. Between 2003 and 2005, 8 children(4 to 13 years old) with soft tissue defects of the extremities were reconstructed with TDA & ALT PFFs. All flaps completely survived. There were no problems relating to vascular spasm or occlusion. Range of motion of reconstructed extremities were fully recovered. Among 8 patients, only 1 patient was performed an additional flap thinning procedure. 8 cases of TDA & ALT perforator flaps were successfully transferred in children. PFF is an excellent option in reconstruction of children as well as in adults because of its thinness, long pedicle length, least donor site morbidity and acceptable donor site scar.

• 한국항해학회지
• /
• 제1권1호
• /
• pp.17-26
• /
• 1977

A large number of the set-nets are set in Namhaedo coast of Korea. The floats of these set-nets are not only small even in case of large floats but also they scarcely have distinguishable marks such as light buoys or flags, so that they are very hard to be recognized by naked eyes and thus became probable obstacles to navigation for the passing ships and the fishing vessels. In order to research the capability of detecting such nets with Radar, the author investigated a maximum detectable range of the ordinarly large floatsand of a floating corner reflectors of various size and shape by Radar. The results obtained are as follows; 1. A maximum detectable range of large floats at a close range can be calculated by the Radar equation in sufficient accuracy. 2. Large floats of the large set-nets are also detectable by Radar even though it's detectable range boundary was within 0.2-0.65 miles. And the Radar picture of large floats was easier to be found with somewhat higher setting of the gain control on shorter range scale of the 1 mile. 3. Floating corner reflector rather suitable for set-net floats of "S" type reflector proposed in this paper, of which the dimension must be above 17cm in diameter to be detectable by Radar at 2 miles.t 2 miles.