• 정보관리학회지
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• 제36권1호
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• pp.169-190
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• 2019

본 연구는 고등학교 공통 과목을 중심으로 교과 교사들의 2015 개정 교육과정 현장 적용 실태와 인식이 교육정보요구에 미치는 영향을 확인하는 데 목적이 있다. 이를 위하여 인천광역시 일반계 공립 고등학교에 재직 중인 국어, 수학, 영어, 사회, 과학 교과(군) 교사를 대상으로 개별 심층 면담과 설문조사를 실시하였다. 분석 결과, 2015 개정 교육과정 현장 적용 실태와 인식은 교육정보요구에 일부 유의한 영향을 미치고 있음을 확인하였다. 특히 교육정보요구 부분에서 2015 개정 교육과정 적용에 따른 새로운 복본 요구 형태와 학습자료 정보원 요구 양태를 발견하였으며, 이를 바탕으로 지역적 범위 내 소규모 학교도서관 컨소시엄 형성과 레퍼럴 서비스 제공, 게이트웨이 역할 수행 기능 강화 등의 학교도서관 운영 방안을 제안하였다.

• 교육시설
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• 제13권2호
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• pp.42-49
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• 2006

We have analysed the use and organization of space in the Centres des Documentations et de Informations(CDI) in lycees in France with the equivalent information centres. The majority of CDI are located in close proximity to the Academic staff and provide easy student access. The CDI provide not only books but also reviews, magazines and CD-Rom. This information is very important to pupils both in the pursuit of their higher studies and also with their future professional life as there is also careers information available to be discussed regularly with the careers advisor. The function of the CDI is not only to provide information. As part of the curriculum there are classes on how to access the information available in the CDI as well as Seminars and Audio-Visual courses. This management makes it possible for the CDI to operate, not only as a school library but also as a multi-function centre of documentation and information; a variety of spaces are also available: a room for private/small group study. A computer room (as well as a research corner), a reading room, monthly review room, photocopy room, rest room, exhibition room, careers information room, audio visual room etc. The results of this study can be used as essential information during the space planning of Korean school libraries in the future.

• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.1092-1096
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• 2020

YjiC, a glycosyltransferase from Bacillus licheniformis, is a well-known versatile enzyme for glycosylation of diverse substrates. Although a number of O-glycosylated products have been produced using YjiC, no report has been updated for nucleophilic N-, S-, and C- glycosylation. Here, we report the additional functional capacity of YjiC for nucleophilic N- and S- glycosylation using a broad substrate spectrum including UDP-α-D-glucose, UDP-N-acetyl glucosamine, UDP-N-acetylgalactosamine, UDP-α-D-glucuronic acid, TDP-α-L-rhamnose, TDP-α-D-viosamine, and GDP-α-L-fucose as donor and various amine and thiol groups containing natural products as acceptor substrates. The results revealed YjiC as a promiscuous enzyme for conjugating diverse sugars at amine and thiol functional groups of small molecules applicable for generating glycofunctionalized chemical diversity libraries. The glycosylated products were analyzed using HPLC and LC/MS and compared with previous reports.

• Journal of Plant Biotechnology
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• 제7권1호
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• pp.51-55
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• 2005

Many cloning vectors in which cDNAs can be inserted to the sense orientation have been developed. Uni-ZAP XR vector (Stratagene) should contain clones that are oriented to sense direction with respect to T3 RNA polymerase primer. Unexpectedly, large portions of cDNAs in Chinese cabbage cDNA library showed unusual insertions, antisense orientation and a hybrid of two different clones. Using two clones, 4H03 and 53-B10, derived from different cDNA libraries, we proposed and demonstrated the possibility of unusual-construct formation by in vitro translation and northern blot analysis. The 4H03 clone was inserted with inverse direction, and its transcript and translation product could be produced by T7 RNA polymerase, indicating that this clone is definitely inserted into inverse orientation. The 53-B10 that contains two independent genes was turned out to be a hybrid in which two genes are inserted to opposite direction each other. All unusual constructs might be due to the presence of small fragments of DNA, like adapter. However, the mechanism underlined the formation of unusual constructs is still remain to be solved.

• 천문학회보
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• 제42권1호
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• pp.57.2-57.2
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• 2017

Remote and robotic telescopes are the most effective instrument for astronomical survey projects. The system is based on the dynamic operation of all astronomical instruments such as dome and telescope control system (TCS), focuser, filter wheel and data taking camera. We adopt the ASCOM driver platform to control the instruments through the integrated software. It can convert different interface libraries from various manufacturers into a uniform standard library. This allows us to effectively control astronomical instruments without modifying codes. We suggest a conceptual design of software for automation of a small telescope such as the new wide-field 0.25m telescope at McDonald Observatory. It can also be applied to operation of multi-telescopes in future projects.

• 미생물학회지
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• 제40권4호
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• pp.359-363
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• 2004

자연계에 존재하는 미생물의 대부분이 배양이 불가능하다는 것이 밝혀진 이후, 자연계의 시료로부터 직접 유전자를 클로닝하여 유용유전자를 발굴하는 메타제놈(metagenome) 이용 방법이 주목을 받게 되었다. 그러나 실제로 오염이 심한 환경시료로부터 DNA를 추출하여 유용한 메타제놈 라이브러리(metagenomic library)를 제작하기란 쉽지 않은 일이다. 따라서 본 연구에서는 실제 메타제놈 라이브러리를 제작할 때 만나는 기술적 문제점에 대한 해결 방법을 탐구하였다. 메타제놈 라이브러리 제작에는 fosmid vector가 가장 편리하였으며, 성공적인 라이브러리제작에는 fosmid vector에 클로닝이 가능한 40 kbp 크기의 DNA 조각을 얻는 과정이 중요함을 알았다. 여러 실험 조건을 종합적으로 검사한 후 메타제놈 라이브러리 제작에 대한 최적 방법을 제사하였다.

• 한국문헌정보학회지
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• 제14권
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• pp.93-130
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• 1987

Scientific communication is an information exchange activity between scientists. Scientific communication is carried out in a variety of informal and formal ways. Basically, informal communication takes place by word of mouth, whereas formal communication occurs via the written word. Science is a highly interdependent activity in which each scientist builds upon the work of colleagues past and present. Consequently, science depends heavily on scientific communication. In this study, three mathematical models, namly Brillouin measure, logistic equation, and Markov chain are examined. These models provide one with a means of describing and predicting the behavior of scientific communication process. These mathematical models can be applied to construct quality filtering algorithms for subject literature which identify synthesized elements (authors, papers, and journals). Each suggests a different type of application. Quality filtering for authors can be useful to funding agencies in terms of identifying individuals doing the best work in a given area or subarea. Quality filtering with respect to papers can be useful in constructing information retrieval and dissemination systems for the community of scientists interested m the field. The quality filtering of journals can be a basis for the establishment of small quality libraries based on local interests in a variety of situations, ranging from the collection of an individual scientist or physician to research centers to developing countries. The objective of this study is to establish the theoretical framework for informetrics which is defined as the quantitative analysis of scientific communication, by investigating mathematical models of scientific communication.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2002년도 춘계 학술대회논문집
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• pp.8-15
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• 2002

For the large scale computation of turbulent flows around an arbitrarily shaped body, a parallel LES (large eddy simulation) code has been recently developed in which domain decomposition method is adopted. METIS and MPI (message Passing interface) libraries are used for domain partitioning and data communication between processors, respectively. For unsteady computation of the incompressible Wavier-Stokes equation, 4-step splitting finite element algorithm [1] is adopted and Smagorinsky or dynamic LES model can be chosen fur the modeling of small eddies in turbulent flows. For the validation and performance-estimation of the parallel code, a three-dimensional laminar flow generated by natural convection inside a cube has been solved. Then, we have solved the turbulent flow around MIRA (Motor Industry Research Association) model at $Re = 2.6\times10^6$, which is based on the model height and inlet free stream velocity, using 32 processors on IBM SMP cluster and compared with the existing experiment.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• 생명과학회지
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• 제17권8호통권88호
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• pp.1082-1089
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• 2007

혈청학적 유전자 검색 방법(SEREX)은 암 환자의 면역계를 인식하는 종양 면역유전체(Cancer Immunome)를 형성하는 수많은 종양항원의 발견을 이끌어왔다. 본 연구는 정상인의 고환 조직으로 만들어진 cDNA liabary을 사용하여 폐암환자의 혈청으로부터 40개의 종양항원을 동정하여 그 항원들을 KP-LuT-1부터 KP-LuT-40까지 명명하였다. 이들 항원 중에서 20개는 기존의 다른 종류의 암에서 분리된 것이며 20개는 본 실험에서 새롭게 동정 된 항원들이었다. 유전자 분석을 통하여 분리된 26개의 항원들은 그 단백질의 기능이 알려진 것이었고 14개의 항원들은 기능이 분석되지 않은 유전체의 산물이었다. 이들 항원 중에서 hypothetic단백질 KP-LuT-6는 정상조직에서 제한적으로 발현되었다. RT-PCR에 의한 발현분석 결과에서 16개의 정상조직 중 고환에서만 강력하게 발현 하였고 다른 조직에서는 발현되지 않으나 폐암(3/10), 위암 (3/10) 과 유방암(1/5)들에서 발현 하였다. 이 결과는 KP-LuT-6의 항원이 암 면역치료를 위한 잠재적 유전자로 사용될 수 있는 Cancer/Testis(CT) 항원과 비슷한 유전 자로 사료된다.