• Korean Chemical Engineering Research
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• 제61권4호
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• pp.570-575
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• 2023

Targeted anticancer drug delivery systems are needed to enhance therapeutic efficacy by selectively delivering drugs to tumor cells while minimizing off-target effects, improving treatment outcomes and reducing toxicity. In this study, a silica-based nanocarrier capable of targeting drug delivery to cancer cells was developed. First, silica nanoparticles were synthesized by the Stöber method using the surfactant cetyltrimethylammonium bromide (CTAB). Increasing the ratio of EtOH in the solvent produced uniformly spherical silica nanoparticles. Washing the nanoparticles removed unreacted residues, resulting in a non-toxic carrier for drug delivery in cells. Upon surface modification, the pH-responsive polymer, polyethyleneimine (PEI) exhibited slow doxorubicin release at pH 7.4 and accelerated release at pH 5.5. By exploiting this feature, we developed a system capable of targeted drug release in the acidic tumor microenvironment.

• 한국화재소방학회논문지
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• 제34권1호
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• pp.18-25
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• 2020

본 연구에서는 보온재의 설치 상태를 고려한 ISO 20632와 NFPA 274 시험 기준에 따른 발열량 산출 결과를 분석하였다. 이를 위해서 보온재 총 6종(은박 발포폴리에틸렌(PE(S)), 무은박 발포폴리에틸렌폼(PE(N)), 고무발포(Rubber), 일본-폴리에틸렌폼(PE(J)), 일본-폴리우레탄폼(PU(J)) 그리고 일본-스티로폼(ST(J)))에 대해서 EN 13501-1과 화재성장곡선에 따른 난연 등급을 구분하였다. 그 결과 PU(J), PE(J) 그리고 PE(N)는 Class E 및 Ultra-fast, NFPA 274 시험기준에 의한 PE(S)는 Class D 및 Fast, ISO 20632에 의한 PE(S)는 Class C 및 Slow, 그리고 Rubber와 ST(J)는 Class B 및 Slow로 나타났다. 하지만, 난연 등급 평가 기준인 최대 발열량에 대한 시간평균 기울기는 시험 방법에 따라서 동일 보온재에 대하여 다르게 나타났으며, 보다 정확한 원인을 분석하기 위한 기초 연구가 필요한 것으로 판단된다.

• 한국작물학회지
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• 제46권4호
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• pp.289-295
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• 2001

Experiments were conducted to find out the optimum level of slow release N fertilizers when total amounts of nitrogen required throughout the growing season in paddy were applied in the soil of seedling box. To evaluate the emergence rate and growth of rice seedlings, five levels of Meister (MS) 10, MS S10, and latex coated urea (LCU) which are equivalent to 0, 40, 60, 80, and 100kg N h $a^{-l}$ were mixed in soil of the seedling box. Emergence rate differed depending on the fertilizers and N levels; in MS 10 plots the emergence rate was 40.8% at 40kg N h $a^{-l}$ and no seedlings were emerged at the higher levels, in MS S10 plots higher than 80% at all the N levels, and decreased with the N levels from 70.0% at 40 kg N h $a^{-l}$ to 59.5% at 100kg N h $a^{-l}$ of LCU. Seedling started to wilt at 40 kg N h $a^{-l}$ of MS 10 and 80 and 100 kg N h $a^{-l}$ N of LCU on the 8th day after sowing, while seedling growth was normal at all the levels of MS S10. Field performance of rice was evaluated at the 0, 30, 60, 90, 120kg N h $a^{-l}$ of MS S10 applied in the soil of seedling box and N was not applied in paddy. Grain yield at 90 and 120kg N h $a^{-l}$ of MS S10 was similar to conventional urea split application (120 kg N h $a^{-l}$), but significantly higher compared to 30 and 60kg N h $a^{-l}$ of MS S10. Fertilizer N recovery decreased with N levels and the N recovery at 90 kg N h $a^{-l}$ of MS S10 and conventional urea split application were 62.2 % and 44.2%, respectively, with similar grain yield. The optimum level of MS S10 to be applied in seedling box seems to be about 90 kg N h $a^{-l}$ considering grain yield, price of fertilizer, labor applying fertilizer, and fertilizer N recovery.d fertilizer N recovery.

• 아시안잔디학회지
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• 제23권1호
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• pp.111-122
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• 2009

본 연구는 완효성 질소 성분이 함유된 복합비료의 시비가 크리핑벤드그래스 생육과 토양 중 변화에 미치는 영향을 시험하고자 2007년 7월부터 12월까지 6개월간 수행하였다. 처리구는 비료를 처리하지 않은 무처리구(NF), 속효성질소복합비료(11-5-7)를 처리한 대조구(CF), MU 질소성분이 함유된 복합비료를 처리한 처리구 1(MU), IBDU 질소성분이 함유 된 복합비료를 처리한 처리구 2(IBDU 1)과 처리구3(IBDU 2) 등 5개 처리를 난괴댑으로 수행하였다. 시비 후 속효성질소성분과 완효성질소성분의 경과시간에 따른 토양 중 변화와 염색지수와 엽록소함량과 같은 잔디품질과 잔디 생육량, 잔디밀도 등 잔디 생육을 조사하였고, 그 결과를 요약하면 아래와 같다. 암모니아태질소와 질산태질소는 모든 처리구에서 시비 후 시간이 경과함에 따라 점차증가하는 경향을 보였고, 총질소는 점차 감소하였다. 질산태질소와 총질소는 속효성 비료처리구(CF)보다는 완효성 비료 처리구(MU, IBDU 1, IBDU 2)에서 높게 나타났으며, 시비 후 경과시간에 따른 토양 중 가용성질소의 변화는 시비초기에는 속효성 비료의 가용성 질소가 많으나 시비 후 30일 정도까지는 속효성 비료와 완효성 비료의 가용성 질소 비율은 비슷하고 30일 이후에는 완효성 비료 처리구에서 가용성 질소 비율이 증가하였다. 염색지수와 엽록소함량을 NF와 비교한 결과, 엽색지수는 CF, MU, IBDU 1 및 IBDU 2에서 각각 6.5%, 6.7%, 5.9%, 5.5% 증가하였고, 엽록소함량은 33.2%, 38.4%, 35.1%, 37.1% 증가하여 MU 처리구에서 높게 나타났다. 잔디생육량은 CF, MU, IBDU 1 및 IBBU 2가 NF보다 76.2%, 77.7%, 69.5%, 72.3% 각각 높게 나타났으나 대조구와 처리구간 예초물량은 비슷하였고, 잔디밀도는 무처리구보다 비료 처리구에서 증가하였고, 속효성 비료처리구(CF)보다 완효성 비료 처리구(MU, IBDU 1, IBDU 2)에서 잔디밀도가 증가했다. 본 결과들을 통해 완효성 질소 성분함유비료가 속효성 질소에 비해 토양 중 가용성 질소의 함량을 높여 질소비효기간을 연장시키고, 염색지수와 엽록소함량은 비슷하며 예초물량은 비슷하거나 감소시키지만 잔디밀도는 향상시켜 완효성질소 함유비료의 시비가 잔디생육에 효과적인 것으로 평가되었다.

• 한국운동역학회지
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• 제14권1호
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• pp.51-63
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• 2004

The purpose of this study was to investigate a three dimensional kinematic variables about the last stride and the release phase of the throwing technique for female javelin throwers. For the motion analysis, Six female javelin throwers were used as subjects. Three-dimensional coordinates were collected using the Kwon3D Motion Analysis Package Version 2.1 Program. Two S-VHS Video Cameras were used to record the locations and orientations of control object and the performances of the subjects at a frequency of 6.0 HZ. After the kinematic variables such as the time, the distance, the velocity, and the angle were analyzed about the last stride and release phase, the followings were achieved; 1. For the effectively javelin throwing, the subjects appeared to do long the approach time in the phasel of landing phase, and short the delivery time in release phase 2. In the release event, the other subjects except for subject A appeared to throwing in the lower condition than the height of themselves. This result showed to slow the projecion velocity. 3. For increase the projection vcelocity of the upper extremity joint in the release event, it appeared to do extend rather the shoulder angle than increase the extension of elbow joint. 4. The body of COG angle showed to gradually increase nearly at the vertical axis in the release event. But the front lean angle of trunk showed a small angle compare to increase of the body of COG angle. Therefore for the effectively momentum transmission of the whole body in the javelin, the front and back lean angle of trunk appeared to do fastly transfer the angle displacement in the arch posture or the crescent condition during the deliverly motion of the release phase.

• 대한치과교정학회지
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• 제22권1호
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• pp.273-283
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• 1992

To find out the differences between periodontal ligament cells (PDL cells) and gingival fibroblast cells (GFB cells), alkaline phosphatase, a marker enzyme for osteoblast, was used to measure the activities and $^{45}CaCl_2$ isotope was used to find out cellular and release of $^{45}Ca$, a requisite for bone formation,. PDL cells and GFB cells from 1 to 5 passages were also measured in alkaline phosphatase activity assay. By the use of above methods, followings were concluded that the PDL cells and the GFB cells have characteristics that are different from each other. In that PDL cells showed large amount of calcium uptake and large amount of calcium release in initial stage, they seem to possess characteristics which are similar to osteoblast-like cells. 1. The PDL cells, in contrast to the gingival fibroblast, showed exceedingly high alkaline phosphatase activity which was highest at the second passage, decreasing thereon. But gingival fibroblasts cells showed no distinct differences in alkaline phosphatase activity as the passage were elapsed. 2. For both PDL cells and GF cells, the $^{45}Ca$ uptake was greatest at 2 hours period. The PDL cells showed higher measuring than GFB cells through out the whole time period. 3. Whereas the GFB cells showed slow increase of $^{45}Ca$ release as time relapsed, the PDL cells showed rapid increase of $^{45}Ca$ release.

• International Journal of Industrial Entomology and Biomaterials
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• 제28권2호
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• pp.66-70
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• 2014

In order to the feasibility of silk materials as protein delivery system, silk beads incorporated with bovine serum albumin (BSA) were prepared by dropping silk fibroin extract into dope solution composed of ethanol and dichloromethane. Structural and morphological characteristics of silk beads were examined using scanning electron microscopy (SEM), infrared spectrometry, and X-ray diffractometry. Swelling ratio of silk beads was also measured. Release behavior of prototypical protein, BSA, was studied by observing the electropheretic phenomenon and release profile. SEM showed that silk beads are spherical with porous interior structure. Infrared spectrometry and X-ray diffraction confirm that the silk beads have a ${\beta}$-sheet conformation. The swelling capability of silk beads increased with the incorporation of the protein. The protein was released from the beads with slow release following an initial burst release. Therefore, silk beads show promise as materials for encasing protein drugs to be delivered to targets in the human body.

• Bulletin of the Korean Chemical Society
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• 제32권3호
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• pp.867-872
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• 2011

For the prolonged delivery and sustained release rates of low molecular weight drugs, poly(lactic-co-glycolic acid) (PLGA) microparticles containing the drug SKL-2020 have been investigated. On increasing polyvinyl alcohol (PVA) concentration (from 0.2% to 5%), the size of microparticles decreased (from $48.02{\mu}m$ to $10.63{\mu}m$) and more uniform size distribution was noticeable due to the powerful emulsifying ability of PVA. A higher drug loading (from 5% to 20%) caused a larger concentration gradient between 2 phases at the polymer precipitation step; this resulted in decreased encapsulation efficiency (from 34.19% to 25.67%) and a greater initial burst (from 61.71% to 70.05%). SKL-2020-loaded PLGA microparticles prepared with different fabrication conditions exhibited unique release patterns of SKL-2020. High PVA concentration and high drug loading led to an initial burst effect by rapid drug diffusion through the polymer matrix. Since PLGA microparticles enabled the slow release of SKL-2020 over 1 week in vitro and in vivo, more convenient and comfortable treatment could be facilitated with less frequent administration. It is feasible to design a release profile by mixing microparticles that were prepared with different fabrication conditions. By this method, the initial burst could be repressed properly and drug release rate could decrease.

• Asian-Australasian Journal of Animal Sciences
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• 제27권2호
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• pp.187-193
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• 2014

As a result of the cost of grains, the replacement of grains by co-products (i.e. DDGS) in feedlot diets is a common practice. This change produces diets that contain a lower amount of starch and greater amount of fibre. Hypothetically, combining feed grade urea (U) with slow release urea (Optigen) in this type of diet should elicit a better synchrony between starch (high-rate of digestion) and fibre (low-rate of digestion) promoting a better microbial protein synthesis and ruminal digestion with increasing the digestible energy of the diet. Four cannulated Holstein steers ($213{\pm}4$ kg) were used in a $4{\times}4$ Latin square design to examine the combination of Optigen and U in a finishing diet containing different starch:acid detergent fibre ratios (S:F) on the characteristics of digestive function. Three S:F ratios (3.0, 4.5, and 6.0) were tested using a combination of U (0.80%) and Optigen (1.0%). Additionally, a treatment of 4.5 S:F ratio with urea (0.80% in ration) as the sole source of non-protein nitrogen was used to compare the effect of urea combination at same S:F ratio. The S:F ratio of the diet was manipulated by replacing the corn grain by dried distillers grain with solubles and roughage. Urea combination did not affect ruminal pH. The S:F ratio did not affect ruminal pH at 0 and 2 h post-feeding but, at 4 and 6 h, the ruminal pH decreased as the S:F ratio increased (linear, p<0.05). Ruminal digestion of OM, starch and feed N were not affected by urea combination or S:F ratio. The urea combination did not affect ADF ruminal digestion. ADF ruminal digestion decreased linearly (p = 0.02) as the S:F ratio increased. Compared to the urea treatment (p<0.05) and within the urea combination treatment (quadratic, p<0.01), the flow of microbial nitrogen (MN) to the small intestine and ruminal microbial efficiency were greater for the urea combination at a S:F ratio of 4.5. Irrespective of the S:F ratio, the urea combination improved (2.8%, p = 0.02) postruminal N digestion. As S:F ratio increased, OM digestion increased, but ADF total tract digestion decreased. The combination of urea at 4.5 S:F improved (2%, p = 0.04) the digestible energy (DE) more than expected. Combining urea and Optigen resulted in positive effects on the MN flow and DE of the diet, but apparently these advantages are observed only when there is a certain proportion of starch:ADF in the diet.

• 한국토양비료학회지
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• 제34권6호
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• pp.407-412
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• 2001

본 연구는 건답직파재배에서 완효성질소비료인 Latex coated urea(LCU)와 Meister10(MS10)의 효과를 구명하기 위해 포장시험으로 조생종인 중화벼를 공시품종으로하여 무질소구, 요소구, LCU구, MS구로 처리하여 벼의 생육, 수량 및 수량구성요소, 질소흡수량 및 이용율, 시험전후 토양의 화학적특성을 조사한 결과는 다음과 같다. 초장과 분얼수는 요소구보다 LCU구와 MS10구가 높았고, MS10구는 LCU구보다 초장과 분얼수가 높았다. 입모수는 요소구, LCU구, MS10구간에 통계적 유의성이 없었다. 간장은 요소구 보다 LCU구와 MS10구가 높았으나, 수장은 요소구, LCU구, MS10구간에 통계적 유의성이 없었다. $m^2$당 수수는 MS10구>LCU구>요소구 순이었다. 수량은 MS10구>LCU구>요소구 순이었다. 수확기의 질소흡수량과 질소이용율은 MS10구>LCU구>요소구 순이었다. 시험후 토양에서는 시험전 토양의 화학성은 질소함량에는 변화가 없었으나, pH, 인산 및 규산 함량이 다소 낮아졌다.