Chloride($Cl^-$) channels play critical roles in cell homeostasis and its specific functions such as volume regulation, differentiation, secretion, and membrane stabilization. The presence of these channels have been reported in all kinds of cells and even in frog oocytes. These essential role of $Cl^-$ channels in cell homeostasis possibly play any role in egg homeostasis and in the early stage of development, however, there has been no report about the presence of $Cl^-$ channel in the mammalian oocyte. This study was performed to elucidate the presence of $Cl^-$ channels in hamster eggs. When allowing only $Cl^-$ to pass through the channel of the egg membrane by using impermeant cation such as N-methyl-D-glucamine(NMDG), single channel currents were recorded. These channel currents showed typical long-lasted openings interrupted by rapid flickering. Mean open $time({\tau}o)$ was 43${\pm}$10.14 ms(n=9, at 50 mV). The open probability(Po) was decrease with depolarization. The current-voltage relation showed outward rectification. Outward slop conductance(32${\pm}$5.4 pS, n=22) was steeper than the inward slop conductance(10${\pm}$1.3 pS). Under the condition of symmetrical 140 mM NaCl, single channel currents were reversed at 0 mV(n=4). This reversal potential(Erev) was shifted from 0 mV at 140 mM concentration of internal NaCl(140 mM [Na+]i) to 9.8${\pm}$0.5 mV(n=4) at 70 mM [Na+]i and 11.5${\pm}$1.9 mV at 280 mM [Na+]i(n=4) respectively, strongly suggesting that these are single $Cl^-$ channel currents. To examine further whether this channel has pharmacological property of the $Cl^-$ channel, specific Cl channel blockers, IAA-94(Indanyloxyacetic acid-94) and DIDS(4, 4'-diisothiocyan ostillben- 2-2'disulfonic acid) were applied. IAA-94 inhibited the channel current in a dose-dependent manner and revealed a rapid and flickering block. From these electrophysiological and pharmacological resluts, we found the novel $Cl^-$ channel present in the hamster oocyte membrane. The first identification of $Cl^-$ channel in the hamster oocyte may give a clue for the further study on the function of $Cl^-$ channel in the fertilization and cell differentiation.
The crystallographic and magnetic properties of the system of $Fe_{1-x}Co_x$(x=0.2 and 0.4) prepared by microwave arc-melting with the maximum power of 3.5 kW and a iron-foil with thickness of 25 ${\mu}{\textrm}{m}$ have been studied by the methods of X-ray diffraction and the measurement of the magnetic hysteresis using the vibrating sample magnetometer at room temperature. The samples were prepared in three different ways: First, pellet form pressed under the pressure of 9,000 N/$\textrm{cm}^2$. Second, the sheet cold rolled. Third, thin sheet treated with the temperature of 90$0^{\circ}C$. The X-ray diffraction pattern of the sample prepared by the first method shows that the crystal structure of the sample is bcc as same as that of Fe with a good uniformity. The iron-foil has the coercivity of 43 Oe and the initial slope of magnetization of 0.328 emu/gOe. The coervicity and magnetization of the sample prepared by the second method increased as the Co content increased. But the initial slop of the magnetization decreased as the Co content increased. This means that the displacement of domain wall is suppressed by the increases of coercivity as the Co content increased. The saturation magnetization of the samples made by the third method increased. On the other hand, the coercivity of these samples decreased. The increase of saturation magnetization of the samples seems to be related to the changes in X-ray intensity after heat treatment. Also some magnetic parameters of the samples were calculated by using a simple model and compared with other values.
In this study, the lower limbs joints were analyzed for features based on the biomechanical characteristics of landing techniques according to height and landing on the ground type (flats and downhill). In order to achieve the objectives of the study, changes were analyzed in detail contents such as the height and form of the first landing on the ground at different angles of joints, torso and legs, torso and legs of the difference in the range of angular motion of the joint, the maximum angular difference between joints, the lower limbs joints difference between the maximum moment and the difference between COM changes. The subjects in this study do not last six months did not experience joint injuries 10 males in 20 aged were tested. Experimental tools to analyze were the recording and video equipment. Samsung's SCH-650A model camera was used six units, and the 2 GRF-based AMTI were used BP400800 model. 6-unit-camera synchronized with LED (photo cell) and Line Lock system were used. the output from the camera and the ground reaction force based on the data to synchronize A/D Syc. box was used. To calculate the coordinates of three-dimensional space, $1m{\times}3m{\times}2m$ (X, Y, Z axis) to the size of the control points attached to the framework of 36 markers were used, and 29 where the body was taken by attaching a marker to the surface. Two kinds of land condition, 40cm and 60cm in height, and ground conditions in the form of two kinds of flat and downhill slopes ($10^{\circ}$) of the landing operation was performed and each subject's 3 mean two-way RM ANOVA in SPSS 18.0 was used and this time, all the significant level was set at a=.05. Consequently, analyzing the landing technique as land form and land on the ground, the changes of external environmental factors, and the lower limbs joints' function in the evaluation were significantly different from the slopes. Landing of the slop plane were more load on the joints than landing of plane. Especially, knee extensor moment compared to the two kinds of landing, slopes plane were approximately two times higher than flat plane, and it was statistical significance. Most of all not so much range of motion and angular velocity of the shock to reduce stress was important. In the further research, front landing as well as various direction of motion of kinetic, kinetic factors and EMG variables on lower limbs joints of the study in terms of injury-prevention-approach is going to be needed.
Objectives : The aim was to investigate the characteristics of low dose effect and non monotonic dose responses(NMDRs) and to predict the influence it might have on the health and medicine, traditional Korean medicine. Methods : By investigating recently published major literatures related to low dose effect and NMDRs, the definition, mechanism, major related fields, and the influence on public health and medicine has been categorized and summarized. Results : Although there are still heated debates about the definition of low dose, it implies the biological responses in environmental exposure level and the NDMDRs means the nonlinear relationship between the dose-response in the slope sign change. Also, it implies the new form of the curve showing a U, reverse U shape, and the slop sign constantly changing showing various forms. This mechanism is because the two acceptor shows opposing effects to toxic materials and the affinity is different along with the numerical value that increase and decrease being different. These characteristics generally appear in endocrine disrupters such as bisphenol A, agricultural pesticides, metal, and radiation. The research field in the public health and medical treatment is obesity, problems in metabolism, growth hormone treatment, climacteric treatment, breast cancer, intake of Korean traditional medicine for pregnancy, menopause and phytoestrogen. Conclusions : As a result of discussing implications, NMDRs is a particular effect in low dose and heated debates surround this response, research is being conducted surrounding the field of obesity, problems in metabolism, growth hormone treatment, climacteric treatment, breast cancer, intake of Korean traditional medicine for pregnancy, menopause, and phytoestrogen. More research and interest in needed as it can have a massive influence in the public health and medicine.
Three different types of polycarbonate (PC)/graphene oxide (GO) composites using diphenyl carbonate as a monomer were fabricated by melt polymerization. Those were the PC/GO composite (PC/GO) using a twin extruder, in-situ PC/GO composite (PC/GO-cat.) using a catalyst, and in-situ PC/GO composite (PC/GO-COCl) using a GO-COCl treated by -COCl, Chemical structures of the composites were confirmed by C-H and C=O stretching peak at $3000cm^{-1}$ and $1750cm^{-1}$, respectively. The slope for the storage (G') versus loss (G") modulus plot decreased with an increase in the heterogeneous property of polymer melts. So we can check the GO dispersion of the PC/GO composites using by the slop for G'-G" plot. According to the G'- G" slopes for three different types of PC/GO composites, GO was well dispersed within PC matrix in case of PC/GO and PC/GO-cat.. It was also confirmed by atomic force microscope (AFM) photos. One of the reasons for the poor GO dispersion of PC/GO-COCl is branching and crosslinking processes occurred during polymerization, which was further confirmed by a plot for the complex modulus versus phase difference.
The present study was to survey the site environment, vegetation structure and soil characteristics in the wild habitats of Wikstroemia ganpi in Goheung, Busan and Ulsan, and offers basic information for habitat conservation and restoration. Most of the wild habitats were located at altitudes between 28~242m with inclinations ranged as $0{\sim}40^{\circ}$. The canopy openness was 57.56%. The vegetation structure by the PC-ORD based on the Two Way Cluster Analysis were divided into three groups Community I(Castanea crenata-Pinus densiflora), Community II(Quercus dentata-Pinus thunbergii) and Community III (Pinus thunbergii). The species diversity was 1.3650, and evenness and dominance were found recorded as 0.8666 and 0.1333, respectively. The soil textures were silty loam and sandy loam. The average soil pH was 5.5, electric conductivity was 0.15dS/m, soil organic matter was 2.60% and available phosphorus was 4mg $kg^{-1}$. Correlation coefficients based on environmental factors, vegetation and soil analysis were showed that positive correlations between species diversity and evenness, organic matter and total nitrogen, whereas species slop degree and coverage of herb, diversity and dominance were showed negative correlations.
There have rarely been performed for the clay mineralogy of the East Sea sediments except for few studies about paleoenvironmental aspect. This study inferred the provenance of sediments based on the clay mineral characteristics and distribution pattern for the 120 sediment samples collected by the box corer from the Gangneung-Donghae area between 2017-2019. The relative proportions of the four major clay minerals are abundant in the order of illite, chlorite, kaolinite, and smectite. The continental shelf sediments below water depth 150 m have more chlorite and kaolinite content and better illite crystallinity, but less illite and smectite content, and S/I index than those of continental slope sediments. Clay mineral composition of the continental shelf sediments are influenced by the adjacent continental geology, because north site (Gangneung area) has more chlorite but south site (Donghae area) has more kaolinite. These characteristics and distribution pattern of clay minerals indicate that the provenance of sediments are different between continental shelf and continental slop. The continental shelf sediments may be introduced the study area by the adjacent small rivers whereas the continental slope sediment might be supplied by current from the south of the study area.
The objective of this study was to monitor the edge vegetation development process after timber harvesting at deciduous hardwood forest in Seoul National University Research Forest of Baekwoonsan (Mt), Korea The results from the 4th (2001) and 5th (2003) survey for edge vegetation development process after timber harvesting are summarized as follows. The competitive species at the harvested forest edge are Lindera erythrocarpa, Weigela subsessilis, Lespedeza maximowiczii, Lindera obtusiioba, Arajia eiata. There were Lindera erythrocarpa, Ivelgela subsessilis, Staphyiea bumaida as competitive species at harvested forest border between harvested forest edge and harvested interior. And at the forest interior, the competitive species were Lindera erythrocarpa, Lindera obtusiloba. There were differences in the competitive species at the forest edge due to the differences in direction, altitude, and existing dominant species. But Lindera erythrocarpa, Weigela subsessilis were appeared to be a competitive specieg at the southwestern slope (A site) and northeastern slope (B site). After timber harvesting, Sasa borealisshowed a good growth in early days, but showed a tendency of declining with the developing of canopy layer And coverage was generally higher at the Northeastern slope (Site B) than southwestern slop (Site A). The similarity index was lower as the distance from the forest edge becomes farther.
Journal of the Korean Association of Geographic Information Studies
/
v.17
no.3
/
pp.82-92
/
2014
The ratio of using soil as the main material in construction is quite numerous, and it covers many parts in forms and bases of the structure. Thus, the earthwork forms many structures for social infrastructure, and the stability of these structures is most crucial when completed than under construction. This study executed a field experiment to evaluate the accuracy and utility of the slope, which is an important part in earthwork, when terrestrial LiDAR is obtained, and the results are as follow. First, as the result of the observation using Total Station and terrestrial LiDAR, the horizontal error RMSE was ${\pm}2.2cm$, and the vertical error RMSE was ${\pm}3.0cm$. As the result of the comparison between the errors and permissible range of public surveying regulation, it sufficiently secure the accuracy. Also, the extraction of the check section, which covers the most important part among the stability checks could be scientifically and rationally processed, and these extraction results are expected to be provided as important basic materials for the earthwork slop stability evaluation.
Min, Byung Keun;Kang, In Joon;Park, Dong Hyun;Kim, Byung Woo
Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
/
v.31
no.6_1
/
pp.431-437
/
2013
Landslides are caused by earthquakes or heavy rains. Recently the incidence of landslides has been increased. However, it is impossible to predict the occurrence of landslide exactly. The purpose of this research is that subdivide the classes of elements in the landslide management system by using spatial analysis technique and AHP method. The existing landslide management system is only comprised of weighted value the slope elements without weighted value about the slop direction elements. For the accuracy improvement in landslide occurrence point, weighted value about the slope direction should be considered. This research is focused on segmentation in slope direction three categories. If the direction of landslide does not affect the structure, I do not think the subject is worth considerating. Based on these results will discuss the improvements in Landslides management systems. Analysis results, segmentation on the slope and the slope direction are needed. Segmented categories about topography elements will be increase the accuracy of landslides management system. Also, since topography of the elements is only considered, segmentation of different elements is needed.
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