• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.239-250
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• 1996

The objective of the present experiments were to determine whether micromanipulative and electro-stimulation conditions for blastomere survival overlapped those for oocyte activation in porcine. Eggs selected for in vitro development potential of blastomeres isolated from 4-cell embryos and oocyte activation by electrostimulation were equilibrated for 5~10 min, in 0.3M sucrose solution containing 7.5$\mu\textrm{g}$/ml cytochalasin B, and then electrostimulated for 30$\mu$sec using one pulse of 100, 120, 150 or 180 volts DC with electrodes 0.2mm apart. Single blastomeres were inserted into empty zona pellucida prior to electrostimulaticn. Then they were cultured in 20${mu}ell$ drops of fresh BECM to observe their developmental ability in vitro in a humidified incubat or at 38.5$^{\circ}C$. The results obtained from these experiments are as follows : 1. When one pulse of 100, 120, 150 or 180 volts DC for 30$\mu$sec were applied to porcine oocytes having the slit formed on zona pellucida for activation, activation rates were 65.1, 66.7, 70.7 and 91.7%, respectively. Higher activation rate was observed in 180V. 2. Infact oocytes incubated for 30 min, in 0.3M sucrose solution after electrostimulation were significantally different from control group with increasing of voltages(p<0.05). When voltages used for electrostimulation were increased, activation rates of oocytes were improved in all treatment groups. 3. When zona punctured-oocytes were only electrostimulated, or incubated in 0.3M sucrose solution for 30 min. after electrostimulation at 180 volt DC, activation rates were 90.5 and 95.5%, respectively. And activation rates of zona punctured-oocytes were significantly different from the groups for which zona pellucida was not punctured(P<0.05). 4. When single blastomeres form 4-cell transferred into empty zona pellucida were incubated for 0, 15 and 30 min. in 0.3M sucrose solution after electrostimulation using one pulse of 180 volt DC for 30 $\mu$sec, developmental rates of electrostimulated-single blastomeres to blastocyst were 72.5, 59.0 and 51.2%, respectively, and the ratio of control group developed to blastocyst were 80.0%. 5. The average cell number in electrostimulated-blastomeres developed to blastocyst were 7.9~10.8, and reduced than the cell number in diploid control ; Also cell number decreased with increasing of voltages. The results of these experiments indicate that the optimal condition for achieving in vitro developmental ability of single 4-cell blastomeres and oocyte activatin is 1 pulse, duration 30 $\mu$sec. in 180 volt, and incubation of blastomeres and oocytes in 0.3M sucrose solution after electrostimulation was not significantally different from another treatment groups. The results also show that this condition is suitable for nuclear transplantation using porcine eggs.

• Membrane Journal
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• v.27 no.3
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• pp.216-225
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• 2017

Since the experimental proof of one-atom-thick graphene single layer from graphite in 2004, graphene, as a leading material opening two-dimensional world, has been tremendously investigated owing to its intrinsic extraordinary physical properties. Among many promising graphene applications, it is believed that membranes might be one of the first significant applications for graphene and its derivatives (e.g., graphene oxide). Recently, a number of simulation results and proof-of-concept experimental approaches towards graphene membranes reflect such positive prospects. Moreover, graphene and graphene oxide already show many outstanding intrinsic properties suitable for promising membrane platforms, such as the minimum membrane thickness, excellent mechanical strength, high chemical and thermal stability, and the ability to generate nanopores in the two-dimensional, rigid hexagonal lattices or to create slit-like nanochannels between adjacent sheets. In this paper, important theoretical and experimental developments in graphene or graphene oxide-based membranes for gas separation based on intrinsic properties of graphene and its derivatives will be discussed, emphasizing on transport behavior, membrane formation methods, and challenging issues for actual membrane applications.

• Journal of Korean Society of Steel Construction
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• v.27 no.1
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• pp.77-85
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• 2015

This paper describes the development of force limiting device(FLD). The FLD could induce compressive yield before occurring elastic buckling for slender member under compressive load. Therefore, it might prevent reduction of load carrying capacity by elastic buckling and the structures with the devices would behave stable. A new type of FLD reduced cross area is proposed in this study different to existing studies like as out of plane type, slit type and folded plate type. The parameters of specimens are depth, width and number of cutting. The structural capacity and characteristics of proposed types were verified by experiment and FEM analysis. The FLD of cutting type is efficient in compressive member.

• Journal of The Korean Astronomical Society
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• v.53 no.5
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• pp.103-115
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• 2020

We analyze the spatially resolved kinematics of gas and stars for a sample of ten hidden type 1 AGNs in order to investigate the nature of their central sources and the scaling relation with host galaxy stellar velocity dispersion. We select our sample from a large number of hidden type 1 AGNs, which are identified based on the presence of a broad (full width at half maximum ≳1000 km s^-1) component in the Hα line profile and which are frequently mis-classified as type 2 AGNs because AGN continuum and broad emission lines are weak or obscured in the optical spectral range. We used the Blue Channel Spectrograph at the 6.5-m Multiple Mirror Telescope to obtain long-slit data with a spatial scale of 0.3 arcsec pixel^-1. We detected broad Hβ lines for only two targets; however, the presence of strong broad Hα lines indicates that the AGNs we selected are all low-luminosity type 1 AGNs. We measured the velocity, velocity dispersion, and flux of stellar continuum and gas emission lines (i.e., Hβ and [O III]) as a function of distance from the center. The spatially resolved gas kinematics traced by Hβ or [O III] are generally similar to the stellar kinematics except for the inner center, where signatures of gas outflows are detected. We compare the luminosity-weighted effective stellar velocity dispersions with the black hole masses and find that our hidden type 1 AGNs, which have relatively low back hole masses, follow the same scaling relation as reverberation-mapped type 1 AGN and more massive inactive galaxies.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.3
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• pp.423-431
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• 2002

An experimental study is conducted to investigate the effect of two-frequency forcing on turbulent flow behind a backward-facing step at the Reynolds number of 27000 based on the step height. The forcing is provided from a thin slit located at the edge of the backward-facing step to increase mixing behind the backward-facing step and consequently to reduce the reattachment length. With single frequency forcing, the minimum reattachment length is obtained at the non-dimensional forcing frequency (F) of St$\_$h/ = 0.29. With two-frequency forcing, a subharmonic frequency (F/2) or biharmonic frequency (2F) is combined with the fundamental frequency (F), i.e. (F, F/2) or (F, 2F) forcing is applied. In the case of (F, F/2) forcing, the reattachment length is not much sensitive to the phase difference between F and F/2. However, the reattachment length significantly depends on the phase difference between F and 2F in the case of (F, 2F) forcing. At a certain range of the phase difference, the reattachment length becomes smaller than that of the single frequency forcing.

• Korean Journal of Food Science and Technology
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• v.23 no.2
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• pp.212-216
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• 1991

Whipping and bandaekee making process were known to a bottle neck for yukwa(deep-fat fried waxy rice snack) making process. For mechanization of the process, a machine was designed and manufactured with conveyer. Some functions of the machine were compared. The continuous whipping and bandaekee making machine was developed by modification of chopper. The chopper was substituted with specially designed plates and die. The newly designed plates were suitable for continuous whipping of dough and making bandaekee without showing any quality different at the final stage. The width and thickness of bandaekee could be controlled by speed of conveyer. The proper conveyer speed was 87.3 mm/sec when amount of extrudate of dough was 221.8 g/sec (MW 51%) from chopper. A shape of knife and plate among components of chopper was not seriously influenced on whipping effect. Expectable thickness of bandaekee for good quality was $3.0{\sim}3.5\;mm$. The number of passing through the chopper was not effected on yukwa quality·but no whipping showed bulky volume with too soft texture.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.61.2-61.2
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• 2011

Low-ionization nuclear emission-line regions (LINERs) have been generally regarded to be powered by active galactic nuclei (AGNs), yet still a number of alternative explanations on the origin of LINER emission are suggested; for example, planetary nebulae nuclei of massive stars, supernovae shocks from death of massive stars, and old stellar populations. Interestingly, a majority of recent star formation early-type galaxies (ETGs) in local universe presents such LINER emission lines. Given that situation, revealing the true nature of LINERs is a crucial step to constrain the evolution path to quiescent ETGs. To resolve the issue, we use Keck/LRIS to obtain spatially resolved spectra on a carefully selected ETG. The ETG SDSS J091628.05+420818.7 at redshift z ~ 0.024 shows modest LINER emission line features without any detection of 21 cm radio continuum nor X-ray emission. We perform a stellar continuum subtraction and measure emission line strengths and their uncertainties for each spectrum from five apertures along the slit with size of 1 arcsecond (~0.5 kpc). We find that extended spatial distributions of four emission lines $H{\alpha}$, $H{\beta}$, [OIII]${\lambda}5007$, and [NII]${\lambda}6583$, and they can be explained by central emission blurring effect. We conclude that the emissions seem to be centrally concentrated, indicating the AGN-nature of LINERs.

• Korean Journal of Optics and Photonics
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• v.12 no.2
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• pp.83-90
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• 2001

The Talbot effect for periodic objects with the spatial period p illuminated by expanded coherent light is analyzed by Fresnel diffraction theory, and the Talbot distance (Zr) at which we can observe 1: 1 imaging without any lenses can be defined. We confmned experimentally the Talbot imaging of line, circular, X -type and '||'&'||'copy;-type 2 dimensional alTay gratings at ZT. At the same time, we observed phase reversed Talbot imaging at Zr/2 and Talbot subimage with p/2 at Zr/4 and 3Zr/4. The visibility of Talbot images as a function of the number of slits of the input grating was measured by the FFf (Fast Fourier Transform) results of these images. As a result stationary maximum visibility of V = 0.25 was obtained from grating numbers with more than 15 slit pairs.

• Journal of Radiation Protection and Research
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• v.44 no.1
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• pp.1-7
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• 2019

Background: To monitor proton beam in proton therapy, prompt gamma imaging systems are being developed by several research groups, and these systems are expected to improve the quality of the treatment and the patient safety. To apply the prompt gamma imaging systems into spot scanning proton therapy, the systems should be able to monitor the proton beam range of a spot with a small number of protons ( <$10^8$ protons), which is quite often not the case due to insufficient prompt gamma statistics. Materials and Methods: In the present study, we propose to improve prompt gamma statistics by merging the prompt gamma distributions of several individual spots into a new distribution. This proposal was tested by Geant4 Monte Carlo simulations for a multi-slit prompt gamma camera which has been developed to measure the proton beam range in the patient. Results and Discussion: The results show that the proposed method clearly enhance the statistical precision of beam range measurement. The accuracy of beam range verification is improved, within ~1.4 mm error, which is not achievable before applying the developed method. Conclusion: In this study, we tried to improve the statistics of the prompt gamma statistics by merging the prompt gamma distributions of multiple spots, and it was found that the merged distribution provided sufficient prompt gamma statistics and the proton beam range was determined accurately.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2003.05a
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• pp.91-93
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• 2003

A comprehensive numerical study is carried out to investigate for the understanding of the flow evolution and flame development in a supersonic combustor with normal injection of ncumally injecting hydrogen in airsupersonic flows. The formulation treats the complete conservation equations of mass, momentum, energy, and species concentration for a multi-component chemically reacting system. For the numerical simulation of supersonic combustion, multi-species Navier-Stokes equations and detailed chemistry of H2-Air is considered. It also accommodates a finite-rate chemical kinetics mechanism of hydrogen-air combustion GRI-Mech. 2.11[1], which consists of nine species and twenty-five reaction steps. Turbulence closure is achieved by means of a k-two-equation model (2). The governing equations are spatially discretized using a finite-volume approach, and temporally integrated by means of a second-order accurate implicit scheme (3-5).The supersonic combustor consists of a flat channel of 10 cm height and a fuel-injection slit of 0.1 cm width located at 10 cm downstream of the inlet. A cavity of 5 cm height and 20 cm width is installed at 15 cm downstream of the injection slit. A total of 936160 grids are used for the main-combustor flow passage, and 159161 grids for the cavity. The grids are clustered in the flow direction near the fuel injector and cavity, as well as in the vertical direction near the bottom wall. The no-slip and adiabatic conditions are assumed throughout the entire wall boundary. As a specific example, the inflow Mach number is assumed to be 3, and the temperature and pressure are 600 K and 0.1 MPa, respectively. Gaseous hydrogen at a temperature of 151.5 K is injected normal to the wall from a choked injector.A series of calculations were carried out by varying the fuel injection pressure from 0.5 to 1.5MPa. This amounts to changing the fuel mass flow rate or the overall equivalence ratio for different operating regimes. Figure 1 shows the instantaneous temperature fields in the supersonic combustor at four different conditions. The dark blue region represents the hot burned gases. At the fuel injection pressure of 0.5 MPa, the flame is stably anchored, but the flow field exhibits a high-amplitude oscillation. At the fuel injection pressure of 1.0 MPa, the Mach reflection occurs ahead of the injector. The interaction between the incoming air and the injection flow becomes much more complex, and the fuel/air mixing is strongly enhanced. The Mach reflection oscillates and results in a strong fluctuation in the combustor wall pressure. At the fuel injection pressure of 1.5MPa, the flow inside the combustor becomes nearly choked and the Mach reflection is displaced forward. The leading shock wave moves slowly toward the inlet, and eventually causes the combustor-upstart due to the thermal choking. The cavity appears to play a secondary role in driving the flow unsteadiness, in spite of its influence on the fuel/air mixing and flame evolution. Further investigation is necessary on this issue. The present study features detailed resolution of the flow and flame dynamics in the combustor, which was not typically available in most of the previous works. In particular, the oscillatory flow characteristics are captured at a scale sufficient to identify the underlying physical mechanisms. Much of the flow unsteadiness is not related to the cavity, but rather to the intrinsic unsteadiness in the flowfield, as also shown experimentally by Ben-Yakar et al. [6], The interactions between the unsteady flow and flame evolution may cause a large excursion of flow oscillation. The work appears to be the first of its kind in the numerical study of combustion oscillations in a supersonic combustor, although a similar phenomenon was previously reported experimentally. A more comprehensive discussion will be given in the final paper presented at the colloquium.