• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.4
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• pp.1154-1158
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• 2004

The classical ABC (avidin-biotin peroxidase complex) method for immunohistochemistry in the paraffin-embedded tissues bring into being disadvantage such as low sensitivity of antigen detection and highly background. The biotinyl-tyramide conjugation recently introduced for sensitive immunohistochemistry was applied to light microscopy in paraffin-embedded pancreatic and liver tissues. The protocol consists of an indirect method in which 4-5㎛ tissue sections are reacted successively within a specific primary antibody, followed by a biotinylated secondary antibody, streptavidin-horseradich peroxidase (HRP), and then finally with biotinyl-tyramide. The labeling obtained for insulin and collagen antigen tested in pancreatic and liver tissues, respectively, was found to be highly specific with the labeling for each antigen confined to its particular cellular compartment. In this study, fish (flounder) serum was specially applied to remove nonspecific binding. Background levels and nospecific deposition of the staining were negligible. This results suggest that super-signal induction method by biotinyl-tyramide conjugate can readily applied to antigen detection of the paraffin-embedded tissues.

• The Journal of the Korea institute of electronic communication sciences
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• v.11 no.12
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• pp.1259-1264
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• 2016

Ultrasonic therapy has received great attention in the field of cosmetics related to the treatment of skin thickening because of its fast recovery and safety. In this study, an output circuit of a high intensity focused ultrasound system was developed for the treatment of beauty. To verify the applicability of the high intensity focused ultrasound system to the cosmetic treatment field, we measured and analyzed the 3D ultrasound intensity energy using a hydrophone. And high-intensity focused ultrasound devices were found to be useful for cosmetic treatment.

• Fashion & Textile Research Journal
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• v.8 no.5
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• pp.571-576
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• 2006

Thirty P. acnes were isolated from the 30 acne patients who visited some skin care shops from Feburary of 2001 to August of 2001. They were identified by morphological features (gram stain), biochemical properties. Only P. acne strains are isolated among Propionibacteria sp. and biochemical properities of P. acnes were not different from P. acnes ATCC 11827 strain significantly. The results of the biotyping was as follow: twenty three strains of biotype I from comedone, 1 strain of biotype II from pauple, 5 strains of biotype III from pauple and pustule, and 1 strain of biotype IV from pauple were isolated respectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.3 s.47
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• pp.307-311
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• 2004

In cosmetics industry, we notice that the research should be focused on developing well-being related products such as SPA, esthetique, mass-customization, organics, naturals besides inner beauty and body shaping products. These well-being related products should satisfy customers' expectation about not only concern about health but also safety, reliability, and ethos about products. In that context, the systematic way for well-being related research in cosmetics should be carried out concerning anti-aging with fat-decom-position, vitamin, polyphenol, and organics. At the same time, there are several important factors in this research like augmenting efficacy, controlling skin-absorbency, skin-adaptability, forming, stabilization of activated com-ponents, improving sustainability, and developing new materials based on dermatologic and emotional science. It should be also emphasized that recent technologies such as bio-technology, nano-technology, and natural-material-technology are the cornerstones of developing well-being products.

• Journal of Applied Biological Chemistry
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• v.65 no.2
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• pp.93-99
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• 2022

Lysosome organelle extract (LOE) was derived from egg whites. The lysosome is an intracellular organelle that contains several hydrolysis enzymes. Previous studies have reported that LOE performs important functions, such as melanin de-colorization and anti-melanin production in B16F10 melanoma cells. However, its principal molecular and cellular mechanisms have not been elucidated till date. In non-cytotoxic conditions, LOE significantly inhibited α-MSH induced melanin synthesis of murine B16F10 cells. The anti-melanogenic activity of LOE was mediated by suppressing the mRNA expression of tyrosinase enzyme, tyrosinase related protein-1/2 (TRP-1/2), and microphthalmia-associated transcription factor (MITF) genes. By performing western blot analysis, we found that LOE significantly attenuated melanogenesis. In this case, LOE helped in increasing extracellular receptor kinase (ERK) phosphorylation in α-MSH induced B16F10 cells. Furthermore, MITF is found to be a key regulatory transcription factor in melanin synthesis; it was down-regulated by LOE through ERK phosphorylation. In this experiment, PD98059 (MEK inhibitor) was used to check whether LOE directly regulated the activity of ERK. Although LOE exerted inhibitory effect on melanin synthesis, we could not observe this effect in PD98059-treated α-MSH induced B16F10. These results strongly indicate that LOE is related to ERK activation and MITF degradation in anti-skin pigmentation. Hence, LOE should be utilized as a whitening agent of skin in the near future.

• Journal of the Korea Safety Management & Science
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• v.15 no.3
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• pp.83-91
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• 2013

The purpose of this study was to compare the exposure levels to emotional labor, occupational and socio-psychological stress, and fatigue for workers engaged in the fields of beauty business (make-up, skin, nail, and hair). For this purpose, a survey was carried out and a statistical analysis was performed for 813 persons As a result, emotional labor was found to be linked with 3 factors of emotional control, emotional dissonance, and positive emotional expression. The exposure level of emotional control was found to be high in the area of hair care and that of positive emotional expression was high in the area of nail care. Based on evaluation of Occupational Stress Scale(KOSS), the stress were found to be highest among workers engaged in the field of make-up and lowest among those in the field of nail. The exposure level of psychosocial stress was found to be the highest in the area of make-up and the lowest in the area of nail care. The exposure level of fatigue was found to be the highest in the area of make-up and the lowest in the area of nail care, showing a difference in the exposure level of stress by area of beauty business. The study confirmed that there is a difference in the exposure level related to emotional labor, job stress, psychosocial stress, and fatigue by area of beauty business.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.7
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• pp.4511-4519
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• 2015

I have analyzed the present status of national certifications and qualifications systems in overseas countries in order to come up with improvement alternatives to National Qualifications Framework(NQF). Most national certifications and qualifications systems at home and abroad have introduced, implemented or are in the process of modifying evaluation programs on the basis of Level 8. I have come to the conclusion that certificates and qualification titles equivalent to those for education programs in cosmetology related departments should be developed for high school to the masters and PhD diplomas, at the same time, the certification program to foster industrial engineers as well as master craftsman for skin & nail-care as well as makeup courses that are not available in beauty field, and I expect this study to be used as the basis material for onsite education & training programs for professionals in beauty field whose capability are respected upon acquisition of relevant certificates in relations with cosmetology related education courses.

• Journal of the Korean Society of Fashion and Beauty
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• v.5 no.1 s.12
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• pp.34-50
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• 2007

This study was designed to measure customer awareness about their own scalp and hair treatment based on survey, through the theoretical background of scalp physiology field by considering scalp and hair treatment. This was to provide a principal data about scalp and hair treatment for the beauty industry. Also, the data about the awareness of customers will be used to search for a direction far basic beauty services in the scalp and hair treatment field. Four hundred customers in beauty parlors, dermatology clinics, skin care institutes, and scalp institutes, living in the North Cheolla Province were surveyed. They were 25 years old and over. The surveys were performed over a period of ten days from November 18th to 28th in 2005. The collected data was analyzed by SPSS. Frequency and percentage were used to draw typical feature of subjects. Chi-square test, frequencies, t-test and One-way ANOVA were performed to consider awareness of customers for hair treatment as well as hair character and scalp hair. This study was able to estimate the awareness of customers by putting together between the recognition of scalp based on the special quality of hair and the recognition of hair and scalp treatment. In conclusion, the scalp and hair did not act independently. In order to have healthy hair, one must posses a healthy, and physiologically sound scalp. In order for beauticians to properly serve their clientele who require hair and scalp treatment, one must be able to comprehend and understand the field of scalp and hair treatment.

• Journal of the Korean Society of Clothing and Textiles
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• v.42 no.3
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• pp.544-559
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• 2018

Korea's traditional aesthetic criterion changed with a new makeup culture that followed the social change caused by modernization. Such transitional features are well seen in the cosmetic advertisements of the 1920's and 1930's. To investigate the cultural characteristics and aesthetic-criteria changes of makeup culture at this period, this thesis analyzes cosmetic advertisements carried in newspaper media of the 1920's and 1930's from an aesthetic perspective. This study found that after the late 1920's, more diverse tones were used for face-powder makeup, collapsing the visual, powder-focused makeup which had been considered criteria for beauty, in combination with smelling and tactile senses such as scent or touch. Domestic makeup had the highest value attached to basic skincare in terms of the aesthetic effect via powder makeup; however, Japanese makeup still stressed the importance of color. Besides, particular facts were found as to social significance of makeup acts such as powder users' age group, safety, superiority and rivals of products, and appeal for makeup popularization. This thesis demonstrates how traditional female beauty appears in powder advertisements in the modern period and how it is related to present-day female beauty.

• Journal of Industrial Convergence
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• v.19 no.5
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• pp.151-157
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• 2021

Along with hair beauty, skin beauty, and nail beauty, according to the rapidly changing social flow of industrialization and appearanceism, makeup is acting as an important means for successful social life and smooth human relationships. Accordingly, the realistic need to increase sales of the beauty industry by investigating individual preferred makeup colors from various angles is emerging. Therefore, the makeup interest and color preference according to the pursuit of appearance management were analyzed for women in their teens to 40s living in Gwangju Metropolitan City and Jeollanam-do. As a result of the study, it was found that service workers, students, and teenagers and younger usually wear a lot of makeup. ) was found to be the most used. Through this study, I believe that it will be used as basic data for future makeup product development as well as application data for color selection by makeup artists as well as to increase sales of beauty shops.