• Title/Summary/Keyword: Skin analysis

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Direct Analysis in Real Time Mass Spectrometry (DART-MS) Analysis of Skin Metabolome Changes in the Ultraviolet B-Induced Mice

  • Park, Hye Min;Kim, Hye Jin;Jang, Young Pyo;Kim, Sun Yeou
    • Biomolecules & Therapeutics
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    • v.21 no.6
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    • pp.470-475
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    • 2013
  • Ultraviolet (UV) radiation is a major environmental factor that leads to acute and chronic reactions in the human skin. UV exposure induces wrinkle formation, DNA damage, and generation of reactive oxygen species (ROS). Most mechanistic studies of skin physiology and pharmacology related with UV-irradiated skin have focused on proteins and their related gene expression or single-targeted small molecules. The present study identified and analyzed the alteration of skin metabolites following UVB irradiation and topical retinyl palmitate (RP, 5%) treatment in hairless mice using direct analysis in real time (DART) time-of-flight mass spectrometry (TOF-MS) with multivariate analysis. Under the negative ion mode, the DART ion source successfully ionized various fatty acids including palmitoleic and linolenic acid. From DART-TOF-MS fingerprints measured in positive mode, the prominent dehydrated ion peak (m/z: 369, M+H-$H_2O$) of cholesterol was characterized in all three groups. In positive mode, the discrimination among three groups was much clearer than that in negative mode by using multivariate analysis of orthogonal partial-least squares-discriminant analysis (OPLS-DA). DART-TOF-MS can ionize various small organic molecules in living tissues and is an efficient alternative analytical tool for acquiring full chemical fingerprints from living tissues without requiring sample preparation. DART-MS measurement of skin tissue with multivariate analysis proved to be a powerful method to discriminate between experimental groups and to find biomarkers for various experiment models in skin dermatological research.

A Study of Skin Resistance of Jeong-Acupuncture Points Using the Compound Electrode (복합전극을 이용한 정혈의 최대피부저항특성에 관한 연구)

  • 최진욱;최귀원;박영배
    • The Journal of Korean Medicine
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    • v.24 no.1
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    • pp.100-109
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    • 2003
  • Objectives : The purpose of this study is to 1. measure the skin resistance of jeong-acupuncture points using compound electrode, and 2. develop a database of skin resistance based on age and gender. Methods : The skin resistance of jeong-acupuncture points on the hands and feet of 191 healthy volunteers was measured using a specially designed compound electrode. A constant current of $5{\mu\textrm{A}}$ was applied to the measurement points and the skin resistance was obtained using amplifier, A/D converter and Acknowledge software program, Results : 1. ANOVA statistical analysis showed no significant differences between jeong-acupuncture points on hands and feet. 2. Paired samples t-test showed no significant differences between Yin-Yang meridian points, 3. When the data were sorted by gender, factor analysis showed statistically significant differences between male and female groups in all measured points except for left foot. 4. Based on the factor analysis, a significant relationship between the skin resistance and age was found in feet. However, no such relationship was found in hands. Conclusions : There are differences in skin resistance according to sex and age, Further clinical research is necessary using compound electrode method.

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Extracellular Superoxide Dismutase (EC-SOD) Transgenic Mice: Possible Animal Model for Various Skin Changes

  • Kim, Sung-Hyun;Kim, Myoung-Ok;Lee, Sang-Gyu;Ryoo, Zae-Young
    • Reproductive and Developmental Biology
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    • v.30 no.4
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    • pp.229-234
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    • 2006
  • We have generated transgenic mice that expressed mouse extracellular superoxide dismutase (EC-SOD) in their skin. In particular, the expression plasmid DNA containing human keratin K14 promoter was used to direct the keratinocyte-specific transcription of the transgene. To compare intron-dependent and intron-independent gene expression, we constructed two vectors. The vector B, which contains the rabbit -globin intron 2, was not effective for mouse EC-SOD overexpression. The EC-SOD transcript was detected in the skin, as determined by Northern blot analysis. Furthermore, EC-SOD protein was detected in the skin tissue, as demonstrated by Western blot analysis. To evaluate the expression levels of EC-SOD in various tissues, we purified EC-SOD from the skin, lungs, brain, kidneys, livers, and spleen of transgenic mice and measured its activities. EC-SOD activities in the transgenic mice skin were approximately 7 fold higher than in wild-type mice. These results suggest that the mouse overexpressing vector not only induces keratinocyte-specific expression of EC-SOD, but also expresses successfully functional EC-SOD. Thus, these transgenic mice appeared to be useful for the expression of the EC-SOD gene and subsequent analysis of various skin changes, such as erythema, inflamation, photoaging, and skin tumors.

A Study on the Skin and Eye Testing of para-phenylenediamine and commercial hairdyes containing paraphenylenediamine in animals (실험동물에 대한 para-phenylenediamino을 함유하는 염모제의 피부 및 눈에 대한 시험)

  • Kim, Jung-Jin;Lee, Sun-Woo;Yong, Km-Chan
    • YAKHAK HOEJI
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    • v.38 no.5
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    • pp.562-567
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    • 1994
  • Now para-phenylenediamine(PPDA) is generally used as component of most hairdyes because it can be used more conveniently and effectively than the others. But PPDA become known to cause side effects such as skin sensitization, contact dermatitis and eye irritation. So this study was done to restudy the safety of hairdyes containing PPDA. The results of experiment were as follows. 1. As a result of primary skin irritation test and eye irritation test in white rabbits, the solution containing 5% PPDA(in saline) were classified as weak irritant but four kinds of commercial hairdyes containing PPDA were proved not to have any irritation. 2. As a result of skin sensitization test in guinea pig, four kinds of commercial hairdyes were classified as Calss I(week irritant) but three kinds of commercial hairdyes induced skin sensitization for guinea pig to 5% in test groups. These results indicate that the actual toxicity of commercial hairdyes(four kinds) is negligible. But based on the results of experiment, it is thought that there is possibility of some commercial hairdyes to induce skin sensititization.

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The Ratios of Pre-emulsified Duck Skin for Optimized Processing of Restructured Ham

  • Shim, Jae-Yun;Kim, Tae-Kyung;Kim, Young-Boong;Jeon, Ki-Hong;Ahn, Kwang-Il;Paik, Hyun-Dong;Choi, Yun-Sang
    • Food Science of Animal Resources
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    • v.38 no.1
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    • pp.162-171
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    • 2018
  • The purpose of this study was to investigate the quality of duck ham formulated with duck skin through the pre-emulsification process. The experiments to investigate the quality characteristics of duck ham were carried out to measure proximate composition, cooking loss, emulsion stability, pH, color, texture profile analysis, apparent viscosity, and sensory characteristics. Duck ham was prepared with various ratios of duck skin in pre-emulsion as follows: Control (duct skin 30%), T1 (duck skin 20% + pre-emulsified duck skin 10%), T2 (duck skin 15% + pre-emulsified duck skin 15%), T3 (duck skin 10% + pre-emulsified duck skin 20%), and T4 (pre-emulsified duck skin 30%). As the ratio of duck skin to pre-emulsified skin changed, the quality of duck ham in terms of moisture content, fat content, cooking loss, emulsion stability, lightness, textural analysis, apparent viscosity, and overall acceptability changed. The moisture content of T2 was the highest (p<0.05) and that of the control and T4 was the lowest (p<0.05). The fat content of control was higher than all treatments (p<0.05). T2 had the lowest values in cooking loss, total expressible fluid, fat separation, hardness, springiness, and gumminess (p<0.05). The score of overall acceptability of all treatments with pre-emulsified skin was higher than control (p<0.05). Therefore, the pre-emulsification process can improve the quality characteristics of duck ham and 1:1 ratio of duck skin and pre-emulsified skin was the proper ratio to improve the quality characteristics of duck ham.

Development of big data based Skin Care Information System SCIS for skin condition diagnosis and management

  • Kim, Hyung-Hoon;Cho, Jeong-Ran
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.3
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    • pp.137-147
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    • 2022
  • Diagnosis and management of skin condition is a very basic and important function in performing its role for workers in the beauty industry and cosmetics industry. For accurate skin condition diagnosis and management, it is necessary to understand the skin condition and needs of customers. In this paper, we developed SCIS, a big data-based skin care information system that supports skin condition diagnosis and management using social media big data for skin condition diagnosis and management. By using the developed system, it is possible to analyze and extract core information for skin condition diagnosis and management based on text information. The skin care information system SCIS developed in this paper consists of big data collection stage, text preprocessing stage, image preprocessing stage, and text word analysis stage. SCIS collected big data necessary for skin diagnosis and management, and extracted key words and topics from text information through simple frequency analysis, relative frequency analysis, co-occurrence analysis, and correlation analysis of key words. In addition, by analyzing the extracted key words and information and performing various visualization processes such as scatter plot, NetworkX, t-SNE, and clustering, it can be used efficiently in diagnosing and managing skin conditions.

Application of Compensation Method of Motion Analysis Error Using Displacement Dependency between Anatomical Landmarks and Skin Markers Due to Soft Tissue Artifact (연조직 변형에 의한 해부학적 지표와 피부마커의 변위 상관성을 이용한 동작분석 오차 보정 방법의 적용)

  • Ryu, Taebeum
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.35 no.4
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    • pp.24-32
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    • 2012
  • Of many approaches to reduce motion analysis errors, the compensation method of anatomical landmarks estimates the position of anatomical landmarks during motion. The method models the position of anatomical landmarks with joint angle or skin marker displacement using the data of the so-called dynamic calibration in which anatomical landmark positions are calibrated in ad hoc motions. Then the anatomical landmark positions are calibrated in target motions using the model. This study applies the compensation methods with joint angle and skin marker displacement to three lower extremity motions (walking, sit-to-stand/stand-to-sit, and step up/down) in ten healthy males and compares their performance. To compare the performance of the methods, two sets of kinematic variables were calculated using different two marker clusters, and the difference was obtained. Results showed that the compensation method with skin marker displacement had less differences by 30~60% compared to without compensation. And, it had significantly less difference in some kinematic variables (7 of 18) by 25~40% compared to the compensation method with joint angle. This study supports that compensation with skin marker displacement reduced the motion analysis STA errors more reliably than with joint angle in lower extremity motion analysis.

Elevated Aurora Kinase A Protein Expression in Diabetic Skin Tissue

  • Cho, Moon Kyun;An, Je Min;Kim, Chul Han;Kang, Sang Gue
    • Archives of Plastic Surgery
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    • v.41 no.1
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    • pp.35-39
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    • 2014
  • Background Aurora kinase A (Aurora-A) plays an important role in the regulation of mitosis and cytokinesis. Dysregulated Aurora-A leads to mitotic faults and results in pathological conditions. No studies on Aurora-A expression in human diabetic skin tissue have been reported. In light of this, we explored the expression of Aurora-A in human diabetic skin tissue. Methods Aurora-A protein was evaluated by western blotting in 6 human diabetic skin tissue and 6 normal skin specimens. Results Increased expression of Aurora-A protein was detected in all diabetic skin tissue samples in both western blot analysis and immunohistochemical staining. However, in the case of the normal skin tissue, no bands of Aurora-A protein were detected in either the western blotting analysis or the immunohistochemical staining. Conclusions Thus far, there have been no studies on the expression of Aurora-A in diabetic skin tissue. However, we believe that oxidative DNA damage related to the expression of Aurora-A protein and Aurora-A could be involved inhuman diabetic skin tissue.

Effects of Tomato Extracts on Detergent-Induced Dry Skin in Rats (토마토추출물의 흰쥐 건성피부에 미치는 효과)

  • Na, Hyun-Sook;Kim, Jong-Bong
    • Journal of Life Science
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    • v.18 no.4
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    • pp.441-446
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    • 2008
  • Dry skin, called xerosis as medical term, is one of the most common skin problems. Many epidemiological studies show that the consumption of foods containing lycopene plays an important role in protecting the epithelial tissue. In this study, water extracts of tomato (WET) containing lycopene were fed and applied to evaluate the effects on dry skin induced by kitchen detergent in rats. These effects were identified by protein analysis and histological changes such as inflammatory erythematic skin as well as acanthosis. The visual scoring for skin observation showed the value such as 4 indicating fiery red with edema after detergent application to skin for 3 wk. However, WET feeding and application to skin showed the decreased values, from 0.7 to 1.0. In addition, it was noteworthy that the epidermis of dry skin show apparent acanthosis with abnormally accentuated keratinization and parakeratosis. However, acanthosis was reversed by feeding and application of WET to dry skin. In order to analyze the effects of WET on dry skin induced by detergent, protein analysis was carried out. The increased amount of protein in dry skin after WET feeding and application would be suggested as one of biochemical mechanisms for recovering the damaged skin. Thus, it would be recommended that water-extracted tomato is a new ingredient in skin regeneration from dry skin induced by detergent.

A Clinical Research of Atopic Dermatitis treated by Yeongyuseungmatang in cosmetics (연교승마탕(連翹升麻湯)이 함유된 한방화장품이 아토피 피부염에 미치는 임상 연구)

  • Kim, Sung-Ho;Yun, Dae-Chul;Kim, Hee-Taek;Ho, Dong-Su;Yoon, Kyung-Sup
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.21 no.2
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    • pp.126-141
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    • 2008
  • Objective : This clinical research was conducted to test patients with Atopic Dermatitis by external application with Yeongyuseungmatang in cosmetics. Methods : We gave scores to 33 patients who visited the Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology of Semyung Jecheon Oriental Medical Hospital from April 17th, 2008 to June 12th. Seventeen patients were treated with Yeongyuseungmatang(Experimental group) and sixteen patients were treated with Atopico skin care cream(Control group) for 4weeks. We observed change of total IgE, eosinophil count, Skin Temperature, Transepidermal Water Loss(TEWL), Skin Hydration and Skin pH. Also Clinic Index of Atopic Dermatitis(SCORAD Index) and global assesment of efficacy were used to evaluate the effects of Yeongyuseungmatang. Statistical analysis was performed by using frequency analysis and descriptive analysis. Statistical significance was achieved if the probability was less than 5%(p<0.05). Results : 1. After 4 weeks of external application treatment, SCORAD Index in Yeongyuseungmatang was significant statistically decreased compared with control group. 2. After 4 weeks of external application treatment, eosinophil count in Yeongyuseungmatang was significant statistically decreased compared with control group but total IgE was very increased both Yeongyuseungmatang and control group. 3. Statistically, Yeongyuseungmatang and control group didn't showed significant effect on Skin Temperature was measured by Thermometer SM815. 4. Transepidermal Water Loss(TEWL) in Yeongyuseungmatang was significant statistically decreased compared with control group measured by Tewameter TM300. 5. Skin Hydration in Yeongyuseungmatang was significant statistically increased compared with control group measured by Corneometer CM825. 6. Statistically, Yeongyuseungmatang and control group didn't showed significant effect on Skin pH was measured by Skin-pH-Meter PH905. 7. After 4 weeks of external application treatment, Yeongyuseungmatang showed significant effect on global assesment of efficacy. Conclusion : Considering the above results, we can speculate that cosmetics with Yeongyuseungmatang has some therapeutical effects in mitigating the symptoms of atopic dermatitis.

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