• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.86-86
• /
• 2003

Research has been in progress for more than a decade to production of useful proteins by genetic modification in cattle. However, the levels of protein production in transgenic cattle have been reported very low. To enhance protein production in transgenic animal, we tried homologous recombination to donor cells for production of transgenic clone cattle through nuclear transfer procedure. Thus, we constructed the two targeting vectors of human thrombopoietin (TPO) at bovine $\beta$-casein locus using homologous recombination with 13.6 kb and 9.6 kb homology. In two targeting vectors, positive selection was through the neomycin resistance gene and negative selection was by the diphtheria toxin (DT). Gene targeting was attempted in bovine embryonic fibroblasts (bEF) and bovine ear skin fibroblasts (bESF). To determine the most appropriate concentration of neomycin for bEF and bESF, G4l8 resistance was confirmed by culturing the cells in various concentrations of the drug and both of the cells were optimally selected at $900 \mu g/ml$ of neomycin. The transfected bEF and bESF by the targeting vectors were colonized efficiently at the ratio of DNA to transfection reagent such as $4 \mu g$:2 ${mu}ell$ and $1 \mu g$:$2 \mu l$. Comparing number of healthy clones from passage 4 to passage 8, bESF (17%) persist in culture for much longer than bEF (6%). The two gene-targeted bESF clones of 30 random-integrated clones with 9.6 kb homology length were confirmed, however, nothing was out of 72 random integration clones with 13.6 kb homology length, The DT also worked more efficiently in clones transfected with the vector of 9.6 kb homology length. Our data suggests that the choice of donor cell for long culture period should be considered to obtain targeted cell clone, and the gene-targeting frequency and the DT working efficiency are dependent on the length of target homology.

• Journal of Korean Medical Ki-Gong Academy
• /
• v.11 no.1
• /
• pp.236-261
• /
• 2009

'Jeom-hyeol-gigong(點穴氣功)' gives a drill, Gi(氣) as a place to jam. This pathogen(邪氣) is removed. Given the low places and supplement it energy to flow up the well is the cure. This is an internal organ and muscular Gi allows a natural flow. Blood, one that moves and guides Gi is Gi I still feel that it makes any blood, making you feel good in life is flowing with vitality. Gi driving our whole body, while supplying vital energy and blood circulation, helping to defend the body is functioning. 'Jeom-hyeol-gigong' principle of Gi where the blockages to flow naturally energy is to let the flow. Aura of the voluntary and proactive action will be to have healthy bodies. Gi as a whole-body blood circulation leading to the cells in each tissue to supply energy and nutrients to every cell as the original principles of free activities that will maximize your life. Gi to prevent the three causes Internal causes: 5 greed and 7 emotions External causes: climate, food, pathogens, stress, etc. The internal nor the external causes: internal and external factors that cause the complex elements, incorrect position of the bone caused by an imbalance Heart disease will be police officers and raise their resistance to disease than the body, what jung-gi(正氣) have to develop. Beneficial to human body's resistance to raise the jung-gi people young-gi(營氣) and wi-gi(衛氣) should be enhanced. If the form is perfectly possible, Gi cycle itself should not have to breathe. Abdominal diagnosis 'bok-su-ap-an-beop(伏手壓按法)', 'sam-ji-tam-an-beop(三指探按法)' hands are like this, which outlined five viscera in order to understand the problem, the lower side of the clavicle (lung), the pit of stomach (Heart), both the lower ribs (liver), navel below (kidney) can be diagnosed at such areas. In each area of the skin, abdominal muscle tension, aching, or pressing a fuss about, beating the ruling of the state and the problem is a clue. And mo-hyeol(募穴) and certain Acupressure group, the chest, back, belly, so that scattered around each' book 'of the problem can be found. This is also the target of such a diagnosis, such as shape, color of skin, muscle Mostly the scope of the pitch in the cervical spine is broad across the hips. sugi(手氣) method that 'an method(按法) and 'ma method(摩法), bak method(拍法) is.

• Journal of Korean Medicine for Obesity Research
• /
• v.7 no.1
• /
• pp.87-96
• /
• 2007

Objectives : This study was performed to evaluate the effect of oriental medical treatment on aged female obese patient. Methods : We experienced an aged female obese patient. We applied herbal medication(Choweseuncheng-tang), electrolipolysis, auricular acupuncture to her from 26th, March. 2007 to 25th, May. 2007. And we examined the changes of her weight, body composition, body size, skin resistance variability, visual analogue scale of knee pain. Results : 1. Her weight decreased from 73.5kg to 71.1kg and body mass index(BMI) decreased from $29.46kg/m^2$ to $28.11kg/m^2$. 2. Body fat mass(BFM) decreased from 25.7kg to 21.6kg but body muscle mass(BMM) increased from 45.1kg to 46.7kg. 3. Waist-hip ratio(WHR) decreased from 0.96 to 0.92. 4. Basal metabolic rate(BMR) increased from 1,273kcal to 1,314kcal. 5. The circumference of upper extremity decreased 1.03cm and lower extremity decreased 1.30cm but the circumference of the muscle of upper extremity increased 0.41cm and lower extremity increased 0.63cm. 6. The circumference of chest and hip are decreased. In addition to the circumference and fat of abdomen are decreased. 7. The visual analogue scale of knee pain is improved. Conclusions : According to above results, oriental medical treatment could be used safety to aged obese patients without loss of fat free mass. The study is more required to aged obese patient from now on.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.30 no.2
• /
• pp.147-151
• /
• 2004

This study aims at measuring electrical and rheological properties of cosmetic emulsions on the skin under shear flow. The effects of volume ratio and surfactants on structural changes of emulsions were examined by determining the changes of electrical resistance, viscosity, and morphology. As the ratio of the internal phase increased, the phase inversion occurred more quickly. The viscosity change was found to increase with increasing of the variation of electrical resistance of the emulsions. This phenomenon may be caused by decreased resistant force against the shear flow because of the breakdown of the internal phase. Surfactants a]so played a key ro]e on phase transition of emulsions. It is likely that polymeric surfactants anchoring on the emulsion surface reinforced the interfacial mechanical strength. As the concentration of surfactants increased, the phase transition occurred more slowly. It has been demonstrated that the phase changes of emulsions under shear flow can be monitored on the real-time basis by using a JELLI$\^$TM/ chip system, a combination of conductiometry and rheometry. Our approach is expected to a useful experimental tool for predicting the phase transition of the cosmetic products during skin application.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.502-506
• /
• 2009

"Hongyoung" was bred within the potato breeding program of National Institute of Highland Agriculture (NIHA). It was originated from a cross made between Atlantic as a female and AG34314 as a male parent in 2003. "Hongyoung" was evaluated as a clone number Daegwan 1-102 for the growth and tuber characteristics, yielding ability, and resistance to major diseases in the regional yield trials from 2006 to 2007. Finally, this clone was renamed as "Hongyoung" and was presented to Korean Seed & Variety Service in 2007 for registration as a new potato cultivar. "Hongyoung" has a round shape, shallow eye depth, red skinned and fleshed tuber characteristics. Its mean tuber yields from the regional yield trials was 37.4 MT/ha, and it showed the highest yield in spring cropping. "Hongyoung" showed high resistance to common scab (Streptomyces scabies) and potato leaf roll virus (PLRV), however it was susceptible to both potato virus Y (PVY) and late blight (Phytophthora infestans). The dry matter content of "Hongyoung" was low to medium 17.7%, therefore it is suitable for table use. Its anthocyanin content was the highest in autumn cropping with 31.8 mg/100g fresh weight and it contained 3.6 times higher anthocyanin than the control, Jasim.

• Journal of the Korean Applied Science and Technology
• /
• v.32 no.2
• /
• pp.339-347
• /
• 2015

This study is to evaluate moisturizing effect and durability of UV A/B blocking activity with multiple (W/O/W) emulsion system. Most of the sun protective products come to be hot issue having both high SPF and long-lasting activity as using special products when is going out, mountain climbing and sports. Also, many consumers prefer the products which have the excellent waterproofing activity of sun care cosmetics as well as the non-sticky feeling that carried out the study of the sensorial science and texture preference. Therefore, development of the specific formulation using this multiple (W/O/W) emulsion technology, it has O/W type hydro skin feel having soft and moist texture when it is treated on the skin. Finally, this formulation is instantly changed to W/O type feel after adsorbed into the skin. The purpose of this study is to get high SPF lasting effect having high water resistance tactivity with high functional multiple (W/O/W) emulsion cream. We used major ingredients, UV-B absorbers were selected with ethylhexyl methoxycinnamate, isoamyl-p-methoxycinnamate, ethylhexylsalicylate, and octocrylene, UV-A absorbers were selected with butylmethoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxy phenyltriazine. SPF effect of O/W type cream was 34.1. SPF effect of W/O/W type cream was 40.6 (increased about 19%). Water resistance effect after 4 hours, SPF effect of O/W type cream was 3.6 (quickly drop down). SPF effect of W/O/W type cream having 81.0 % waterproofing effect was 32.7 (decreased about SPF 7.9). Moisturizing effect of O/W cream at first was superior comparing multiple emulsion. But after 3 hours quickly was drop-down. Moisturizing effect of multiple emulsion was high comparing O/W type and other sun block creams after 4 hours was constantly maintaining water-content.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.36 no.10
• /
• pp.1050-1057
• /
• 2012

This study helps develop a cool body armor that maintains a tight-fit configuration to the body surface and evaluates the performance of newly developed body armor in a wear test. Three types of body armor were used for evaluation. One was a tight fitting body armor that was constructed to improve the degree of fit and ease of movement for Korean soldier using 3D technology. Another was ventilating body armor with attached spacers on the shoulder to reduce the thermal stress on the soldier. The third was a prevailing body armor produced by a Korean body armor company. In order to evaluate the performance of the body armor, a human wear test, a thermal mannequin test, and computational fluid dynamics (CFD) were executed. Five subjects participated in the wear test. Subjective wear sensation, total amount of sweat and dynamic change of clothing microclimate were observed during and after exercise on a treadmill; subsequently, it was found that subjects rated tight fitting body armor and ventilating body armor lighter, drier, and easier to move than the conventional body armor (p<.05). Total amount of sweat was the least in the case of ventilating body armor. The thermal resistance and vapor resistance of the ventilating body armor were improved remarkably. In addition, the skin temperature of the ventilating body armor with spacers was lower than the tight fitting body armor by at least $1^{\circ}C$ in the CFD result. It is noted that thermal-wet comfort of the 3D body armor with ventilating feature is superior to the conventional body armor, especially when the ventilating channel is not closed due to a backpack.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.27 no.3
• /
• pp.273-279
• /
• 2013

The objective of this study is to find out a reason why prescriptions have different effects on each patient who has same symptoms or same abdominal examinations and further more to classify the formulas more efficiently. In applying 's prescriptions, it is known that conventional ways such as treatment in accordance with symptoms or abdominal examinations have many advantages and problems reversely. To make up for the problems, additional references like strength of constitutional resistance and location of disease, degree of income and outgo are designed. And the notion in Oriental Medicine embracing aspects mentioned above corresponds to triple energizer. Triple energizer's exuberance-debilitation is able to draw an inference from some factors like density of skin interstices, elasticity of abdomen, appetite, digestive power. According to Exuberance-Debilitation of Triple Energizer, can be divided into five steps: weak(弱)-moderately weak(中弱)-neither weak nor strong(中)-moderately strong(中强)-strong(强). prescriptions would be dealt with those 5 steps, and it would be highly effective and consequently side effects could be reduced. On the basis of this classification method upon formula group, the prescriptions of can be applied more accurately by setting a direction through strength of constitutional resistance and location of disease and combining with existing references like symptoms, palpation and abdominal examinations.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.4
• /
• pp.728-733
• /
• 2005

To elucidate the question of whether biofilm formed by the intercellular adhesion (ica) gene cluster has influences on antibiotic resistance in Staphylococcus epidermidis, we compared 124 skin strains with strains isolated from 50 blood cultures that cause septicemic diseases. The results revealed that the blood culture isolates were more resistant to the antibiotics tested than the saprophytic isolates. Moreover, antibiotic multiresistance was more prevalent in the clinical isolates. In the blood culture isolates, $46\%$ of the strains were resistant to three or more antibiotics, whereas only $12\%$ of the saprophytic isolates were resistant to three or more antibiotics. Interestingly, these characteristics were highly correlated with the biofilm formed by the ica gene cluster. In biofilm-producing strains, $84\%$ of the blood culture isolates and $44\%$ of the saprophytic isolates were antibiotic multiresistant, whereas only $22\%=;and\;9\%$, respectively, were antibiotic multiresistant in biofilm-nonproducing strains. Additionally, in the biofilm-producing ica-positive strains, $89\%$ of the blood culture isolates and $57\%$ of the saprophytic isolates were antibiotic multiresistant. However, the rate of the antibiotic multiresistance in the ica-negative strains was very low, thus indicating that the biofim formed by the lea gene cluster in S. epidermidis is an important pathogenic factor in association with the antibiotic multiresistance.

• Biomedical Science Letters
• /
• v.26 no.3
• /
• pp.244-248
• /
• 2020

Skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus (MRSA) can occur especially in community populations such as military training camps. We investigated antimicrobial resistance patterns and molecular epidemiological characteristics of MRSA isolated from nasal swabs in healthy army trainees. From January 2018 to March 2018, one MRSA strain was isolated from nasal swab and hand of healthy army trainees. mecA gene detection, SCCmec and mec complex typing were performed to analyze the antimicrobial resistance patterns and molecular epidemiological characteristics of MRSA isolates. As a result, SCCmec and mec complex type of MRSA isolate from military trainees was not-typeable (n=1). In conclusion, not-typeable subtype of MRSA isolate from military trainees need to be confirmed by continuous follow-up study to determine whether there is a different genotype or a new subtype of genotype present in the Republic of Korea.