• Journal of Veterinary Science
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• v.25 no.4
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• pp.49.1-49.15
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• 2024

Importance: Endochondral ossification plays an important role in skeletal development. Recent studies have suggested a link between increased intracellular reactive oxygen species (ROS) and skeletal disorders. Moreover, previous studies have revealed that increasing the levels of myeloperoxidase (MPO) and osteopontin (OPN) while inhibiting NADPH oxidase 4 (NOX4) can enhance bone growth. This investigation provides further evidence by showing a direct link between NOX4 and MPO, OPN in bone function. Objective: This study investigates NOX4, an enzyme producing hydrogen peroxide, in endochondral ossification and bone remodeling. NOX4's role in osteoblast formation and osteogenic signaling pathways is explored. Methods: Using NOX4-deficient (NOX4^-/-) and ovariectomized (OVX) mice, we identify NOX4's potential mediators in bone maturation. Results: NOX4^-/- mice displayed significant differences in bone mass and structure. Compared to the normal Control and OVX groups. Hematoxylin and eosin staining showed NOX4^-/- mice had the highest trabecular bone volume, while OVX had the lowest. Proteomic analysis revealed significantly elevated MPO and OPN levels in bone marrow-derived cells in NOX4^-/- mice. Immunohistochemistry confirmed increased MPO, OPN, and collagen II (COLII) near the epiphyseal plate. Collagen and chondrogenesis analysis supported enhanced bone development in NOX4^-/- mice. Conclusions and Relevance: Our results emphasize NOX4's significance in bone morphology, mesenchymal stem cell proteomics, immunohistochemistry, collagen levels, and chondrogenesis. NOX4 deficiency enhances bone development and endochondral ossification, potentially through increased MPO, OPN, and COLII expression. These findings suggest therapeutic implications for skeletal disorders.

• The korean journal of orthodontics
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• v.18 no.2
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• pp.367-385
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• 1988

In case of skeletal Class III malocclusion with underdeveloped maxilla, the extraoral orthopedic force for the stimulation of maxillary growth or anterior reposition of the maxilla has been used clinically for the improvement of facial skeletal relationship. The purpose of this investigation was to examine the initial reaction of maxillofacial complex to the maxillary protraction by using extraoral orthopedic force. The dried human skull was used and this investigation was done by means of double exposure holographic interferometry. The protraction forces placed on the canine or the first molar were parallel, $10^{\circ}$ downward, $20^{\circ}$ downward to the occlusal plane. Fringe pattern of each protraction condition was compared and analized. The results were as follows: 1. Each maxillofacial bone displaced saparately. 2. More displacement was shown at the area of the teeth and the alveolar bone. 3. A counterclockwise rotation of the maxilla wa decreased by downward protraction and especially 20 degree downward protraction from the canine showed least rotation. 4. On the zygomatic arch, outward bend was observed and this effect was decreased by downward protraction. 5. On the zygomatic bone, the counter clockwise rotation was increased by the downward protraction. 6. When maxillary expansion was applied at the same time, outward and upward displacement with counterclockwise rotation was observed on the maxilla. 7. The lateral pterygoid plate of sphenoid bone was affected by maxillary protraction.

• Animal Systematics, Evolution and Diversity
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• v.23 no.1
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• pp.35-38
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• 2007

Two new sponges, Euryspongia coreana n, sp, and E. regularis n, sp., collected from Hataedo, Ulleungdo and Chujado, Korea by SCUBA diving during the period from 2001 to 2005. Euryspongia coreana n. sp, is very close to E. lactea Row, 1911 and E. arenaria Bergquist, 1961 in skeletal structure, but primary and secondary fibres of the new species are thicker than those of E. lactea, E. arenaria is cored with mostly spicule fragments but E. coreana n. sp. is cored with mainly sand. E. regularis n. sp. is readily distinguished from other eurypsongian sponges. by its thickly encrusting growth form, yellowish ivory colour, and thickness of fibres. The bright yellow color and regular ladder-like skeletal structure are major features distinguishing this E. regularis n. sp. from other euryspongian sponges. Most species are brown, violet, red or orange in color except for E. lactea (milky white), E. arenaria (biscuit) and E. coreana n. sp. (yellowish ivory). Other euryspongian sponges have regularly or irregularly well developed secondary fibres, but E. regularis n. sp. has simple and regular secondary fibres.

• Journal of the korean academy of Pediatric Dentistry
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• v.7 no.1
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• pp.75-83
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• 1980

Rickets is not the deposite of minerals in the skeletal tissue and the retardation of skeletal growth in growing in growing animals. This study was undertaken to investigate the histologic effects of experimental rickets on the dental structure of the albino rats, and to show the relationship between the histological effects and the pulpal disease which induced premature loss of the primary teeth. This study was based on material obtained from 40 white rats that were placed on a rachitogenic diet for a period 1 to 56 days after weaning (at 24 days). In addition, a study was made of 25 litter mates, 24 to 80 days, that were fed a normal diet. The following results were obtained: 1. Enamel formation and calcification showed no significant changes and no hypoplasia. 2. Dentin formation and calcification was retarded and disturbed. In the experimental group, predentin/calcified dentin was remarkablly increased. 3. Newly formed dentin showed interglobular texture (less homogenous calcification) and the predentin was significantly wider and thicker, and there was an irregular wave in the basal portion of the rat's incisors. 4. In cementum, Matrix formed at almost a normal rate but calcification was defective. So cementoid tissue was increasesd. 5. The formation of the alveolar bone was at almost a normal rate but calcification was retarded. The trabecular bone was filled with osteoid tissue and thicker than in normal groups.

• The Journal of the Korean dental association
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• v.48 no.11
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• pp.819-828
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• 2010

Activator is a removable functional appliance used for correcting the skeletal Class II malocclusion in children with the mandibular deficiency. Berlin standard activator modified from Andresen activator has following characters; do not cover the palatal surface for tongue space, relief on lingual surface of mandibular incisors and resin capping 1/3-1/2 of crown height on mandibular incisors for preventing labioversion of mandibular incisors, L-hook between maxillary lateral incisor and canine for anterior high pull headgear, relief on mandibular posterior bite block for differential eruption of posterior teeth. Two cases presented here had a mandibular deficiency and slight maxillary protrusion. First case (an 11-year-old girl) treated with Berlin standard activator and anterior high pull headgear for 13 months followed by fixed orthodontic appliance for another 29 months. Second case (a 12-year-old boy) treated with Berlin standard activator for 6 months followed by fixed appliance for another 24 months. Treatment results showed a significant improvement in sagittal skeletal and occlusal relationship without premolar extraction. Mandibular condyles were concentric in TMJ [ossa, and masticatory muscle activities were normalized after treatment. In the retention period facial harmony and occlusal stability was maintained.

• The korean journal of orthodontics
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• v.17 no.1
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• pp.47-54
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• 1987

This study was designed to compare extreme variations in facial growth in order to examine the relationships between the glenoid fossa position and skeletal malocclusions. It was hypothesized that patients with large mandibular plane-sella nasion angles would have a more superior fossa position than patients with small mandibular plane-sella nasion angles. It was also hypothesized that patients with large ANB angles would have a more posterior fossa position than patients with small ANB angle. For this study, the data from lateral roentgenocephaloprams of 72 Females and 72 males aged from 10 to 18 years were used. The results were as follows 1. In the case of large ANB angle, glenoid fossa position was relatively posterior than that of small ANB angle in Female, but it was not significant in male. 2. In the case of large APDI, glenoid fossa position was relatively anterior in female, but it was not significant in male. 3. In the case of large SN-Go Gn angle, glenoid fossa position was relatively superior in both male and female. 4. In the case of large ODI, glenoid fossa position was relatively inferior in both male and female.

• International Journal of Industrial Entomology and Biomaterials
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• v.32 no.2
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• pp.49-53
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• 2016

Bone morphogenetic proteins (BMPs) are essential growth factors for bone formation, skeletal development and bone regeneration. The BMP-2/7 heterodimer is known to have remarkable effects on osteogenic induction that are even stronger than the BMP-2 or BMP-7 homodimers. We designed a recombinant human BMP-2/7 (rhBMP-2/7) heterodimer protein with four glycine residues between BMP-2 and BMP-7 protein to facilitate free bond rotation of domains. The Baculovirus Expression Vector System (BEVS) is routinely used to produce recombinant proteins in the milligram scale. In this study, the BEVS was used to express the rhBMP-2/7 protein whrer the recombinant baculovirus was recovered in the host Sf9 cells. To confirm the biological activity of rhBMP-2/7 protein secreted from the BEVS as an osteogenic differentiation and induction factor, we measured the BMP-induced ALP activity. rhBMP-2/7 could be used as an alternative to BMPs to overcome limitations like short half-life and requirement for high concentrations. Furthermore, rhBMP-2/7 may be an efficient tool for various application studies such as bone regeneration and skeletal development.

• Journal of Genetic Medicine
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• v.19 no.2
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• pp.111-114
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• 2022

Many monogenic neurodevelopmental disorders have been newly identified in recent years owing to the rapid development of genetic sequencing technology. These include variants of the epigenetic machinery - up to 300 known epigenetic factors of which about 50 have been linked to specific clinical phenotypes. Chromodomain, helicase, DNA binding 1 (CHD1) is an ATP-dependent chromatin remodeler, known to be the causative gene of the autosomal dominant neurodevelopmental disorder Pilarowski-Bjornsson syndrome. Patients exhibit various degrees of global developmental delay, autism, speech apraxia, seizures, growth retardation, and craniofacial dysmorphism. We report the first case of Pilarowski-Bjornsson syndrome in Korea, due to a de novo missense variant of the CHD1 gene (c.862A>G, p.Thr288Ala) in a previously undiagnosed 17-year-old male. His infantile onset of severe global developmental delay, intellectual disability, speech apraxia, and failure to thrive are compatible with Pilarowski-Bjornsson syndrome. We also noted some features not previously reported in this syndrome such as skeletal dysplasia and ichthyosis. Further studies are needed to discover the specific phenotypes and pathogenic mechanisms behind this rare disorder.

• The korean journal of orthodontics
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• v.25 no.4
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• pp.415-423
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• 1995

In order to investigate the relationship between the menarche and the bone maturity of growing girls, the author interviewed about the date of menarche of 70 girls and assessed their bone maturity by hand-wrist radiographs. The results and conclusion were as iollows ; 1. The mean menarcheal age was $12.04{\pm}0.82$ year. 2. There was significant correlation between the time interval after menarche and the ossification stage. 3. The skeletal maturation level at menarche was stage SMI 7 and SMI 8. 4. There was statistically different among the time intervals after menarche according to the ossification stage. 5. The epiphyseal union of radius began about 2 years after menarche. According to this study, the probability of clinical use can be accepted in some range. In another words, skeletal age can be predicted by just interviewing the date and time interval of menarche without hand-wrist radiograph, also, with this information, the level of maxillofacial growth can be assessed.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.4
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• pp.224-229
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• 2007

Muscle tissue expresses many muscle-specific genes, including myostatin (also known as GDF8) that is a member of the transforming growth factor-beta superfamily. Myostatin (MSTN) negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. In this study, we first have characterized partial cDNA of a MSTN gene from the muscle tissue in the F. chinensis and examined its expression pattern in various tissues. The partial MSTN gene (GenBank accession number EU 131093) in the F. chinensis was 1134 bp, encoding for 377 amino acids that showed 63-93% amino acid similarity to other vertebrate MSTNs, containing a conserved proteolytic cleavage site (RXRR) and conserved cysteine residues in the C-terminus. Based on a RT-PCR, the MSTN gene was expressed in the all tissues of F. chinensis used in this study.