• Title/Summary/Keyword: Skeletal data

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Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle

  • Lee, Hyun;Kim, Seung-Hyeob;Lee, Jae-Seon;Yang, Yun-Hee;Nam, Jwa-Min;Kim, Bong-Woo;Ko, Young-Gyu
    • BMB Reports
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    • v.49 no.2
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    • pp.116-121
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    • 2016
  • Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.

Genomic Organization, Intronic Duplications, and Promoter Characteristics of the Fast Skeletal Myosin Light Chain-2 Gene (mlc2f) from Javanese Ricefish Oryzias javanicus

  • Lee, Sang Yoon;Kim, Dong Soo;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
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    • v.15 no.4
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    • pp.325-335
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    • 2012
  • The present study characterized the fast skeletal myosin light chain-2 gene (mlc2f) in the euryhaline Javanese ricefish (Oryzias javanicus: Beloniformes). Coding nucleotide and deduced amino acid sequences of Javanese ricefish mlc2f were well conserved in the vertebrate lineage. Javanese ricefish mlc2f showed a typical seven-exon structure, and its promoter exhibited transcription factor binding motifs common to most muscle-specific genes. However, Javanese ricefish mlc2f also displayed tandem duplications of intronic sequences in both intron 1 and intron 3. Based on quantitative reverse transcription-polymerase chain reaction, the mlc2f transcripts were highly predominant in skeletal muscles of adults and were differentially modulated during embryonic development. Microinjection of the mlc2f promoter-driven red fluorescent protein (RFP) reporter construct successfully exhibited heterologous expression of the fluorescent reporter, primarily in muscular areas of hatchlings, although the distribution pattern of RFP signals was not uniform due to the mosaic nature of the introduced transgene. Data from this study indicate that the Javanese ricefish mlc2f gene has undergone "intra-intronic" duplication events in a species-specific manner and that the mlc2f regulator may also be useful in heterologous expression assays of the skeletal muscles of this species.

Association between dietary branched-chain amino acid intake and skeletal muscle mass index among Korean adults: Interaction with obesity

  • Chae, Minjeong;Park, Hyoung Su;Park, Kyong
    • Nutrition Research and Practice
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    • v.15 no.2
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    • pp.203-212
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    • 2021
  • BACKGROUND/OBJECTIVES: The branched-chain amino acids (BCAA), including isoleucine, leucine, and valine, promote muscle protein synthesis. However, obesity may interfere with protein synthesis by dysregulating mitochondrial function in the muscles. This study aimed to examine the association between dietary intake levels of BCAA and skeletal muscle mass index (SMI) in middle-aged participants, and the effect of obesity/abdominal obesity on this association. SUBJECTS/METHODS: The data of 3,966 men and women aged 50-64 years who participated in the 2008-2011 Korea National Health and Nutrition Examination Survey were analyzed. Intake levels of energy-adjusted dietary amino acids were obtained using a 24-hour dietary recall. SMI was calculated by dividing the appendicular skeletal muscle mass by body weight (kg) and multiplying the result by 100%. Multivariable general linear models were used to analyze the association of dietary BCAA intake levels with SMI. RESULTS: The beneficial effects of energy-adjusted dietary BCAA intakes on SMI were greater in the non-obesity/non-abdominal obesity groups; however, no significant associations were observed in the obesity/abdominal obesity groups (P > 0.05). CONCLUSIONS: Healthy weight and sufficient intake of dietary BCAA are recommended to maintain muscle mass.

Associations Between Skeletal Muscle Mass, Grip Strength, and Physical and Cognitive Functions in Elderly Women: Effect of Exercise with Resistive Theraband

  • Kwon, Insu;Kim, Ji-Seok;Shin, Chul-Ho;Park, Yoonjung;Kim, Jong-Hee
    • Korean Journal of Exercise Nutrition
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    • v.23 no.3
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    • pp.50-55
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    • 2019
  • [Purpose] The purpose of this study was to identify the relationships between muscle mass, muscle strength, and physical and cognitive functions and to examine the effects of resistive Theraband® exercise on sarcopenia-associated variables in the older population. [Methods] A total of 28 elderly women (age: 69.90 ± 0.8 years) participated in this study, 15 of whom underwent elastic band exercise for 1 hour per day, twice per week for 8 weeks. The correlation analysis was conducted to identify the associations between body composition, skeletal muscle mass indices, grip strength, and physical and cognitive functions. All variables were assessed at baseline and post-exercise. [Results] Skeletal muscle mass was significantly associated with grip strength and physical function. Gait speed was positively correlated with grip strength and physical function, but not with cognitive function. Theraband® exercise significantly improved gait speed and physical function. [Conclusion] The present data suggest that skeletal muscle mass is highly correlated with grip strength and physical function. Eight weeks of resistive Theraband® exercise favorably affects sarcopenia by improving gait speed and mobility of elderly women.

AMPK activation and adenine nucleotide content in skeletal muscle following different types of high fat feeding

  • Kim, Hyun-Kook;Lee, Songsam
    • Korean Journal of Exercise Nutrition
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    • v.16 no.1
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    • pp.35-41
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    • 2012
  • We investigated the role of fatty acid availability on skeletal muscle AMPK activity and adenine nucleotide content. To investigate the chronic effects of elevated fatty acid in vivo Sprague-Dawley rats were fed a chow diet (15% fat) or a diet high in saturated (SAFA, 52% fat) or polyunsaturated (PUFA, 52% fat) fat for eight weeks. High fat diets increased (P < 0.05) plasma FFA levels by 25%. AMPK activity was increased in SAFA and PUFA rats and occurred in the absence of changes in ATP, AMP, phosphocreatine and glycogen content. These results suggest that increasing fatty acid availability increases AMPK activity independent of changes in the cellular energy charge, and implicate the regulation of AMPK by a covalent mechanism. These data also support the contention that increasing fatty acid availability can increase subsequent fatty acid oxidation by an AMPK-mediated process.

Fall Detection Based on Human Skeleton Keypoints Using GRU

  • Kang, Yoon-Kyu;Kang, Hee-Yong;Weon, Dal-Soo
    • International Journal of Internet, Broadcasting and Communication
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    • v.12 no.4
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    • pp.83-92
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    • 2020
  • A recent study to determine the fall is focused on analyzing fall motions using a recurrent neural network (RNN), and uses a deep learning approach to get good results for detecting human poses in 2D from a mono color image. In this paper, we investigated the improved detection method to estimate the position of the head and shoulder key points and the acceleration of position change using the skeletal key points information extracted using PoseNet from the image obtained from the 2D RGB low-cost camera, and to increase the accuracy of the fall judgment. In particular, we propose a fall detection method based on the characteristics of post-fall posture in the fall motion analysis method and on the velocity of human body skeleton key points change as well as the ratio change of body bounding box's width and height. The public data set was used to extract human skeletal features and to train deep learning, GRU, and as a result of an experiment to find a feature extraction method that can achieve high classification accuracy, the proposed method showed a 99.8% success rate in detecting falls more effectively than the conventional primitive skeletal data use method.

Psychophysical Modeling for Lifting Capacity Using Isometric & Isoinertial Strength Variables (근력을 이용한 최대허용중량 예측 모델에 관한 인체심리학적 연구)

  • Yoon, Hoon-Yong;Chu, Dong-Woo
    • Journal of the Korean Society of Safety
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    • v.24 no.2
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    • pp.89-93
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    • 2009
  • The muscular-skeletal disorders that have become a major issue recently in Korean industrial safety area are mainly caused by manual material handling task. The objective of this study is to provide scientific data for the establishment of work safety standard for Korean workers through the experiments of lifting task under various conditions, in order to prevent the muscular-skeletal disorders in the industrial work site. This study used the psychophysical approach to determine the maximum acceptable weight(MAWL) for seven young male subjects, and used isometric and isoinertial strength variables as predictors to develop prediction models. Also, the oxygen consumption, heart rate, and RPE were measured or recorded while subjects were lifting their MAWL. Three different lifting frequencies(1, 3, 5lifts/min) with two lifting range from floor to knuckle height and knuckle to shoulder height for one hour's work shift using free style lifting technique were studied. These results may not only provide scientific data in establishing the safety standards for Korean workers' lifting tasks, but also contribute preventing the rapidly increasing muscular-skeletal disorders lately on the industrial site.

A Study on Posture Discrimination using Coordinate Transformation of Skeleton Data (골격 데이터의 좌표변환을 이용한 자세판별 연구)

  • Kim, Yong-jin;Noh, Yun-hong;Jeong, Do-un
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.05a
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    • pp.510-511
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    • 2017
  • In this paper, a study was conducted to prevent spinal - related diseases and to help posture correction by feeding back the wrong attitude to the users. Kinect sensor was used for this purpose. In order to measure the movement of the user, the degree of motion change was measured by indexing the skeletal data coordinate value. It is confirmed that the implemented system can observe not only posture but also distraction of user.

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The growth stage study on the femur and tibia of Korean native chicken after hatching (한국 재래닭의 부화 후 성장단계별 넙다리뼈와 정강뼈 성장에 관한 연구)

  • Tae, Hyun-Jin
    • Korean Journal of Veterinary Service
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    • v.38 no.3
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    • pp.181-188
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    • 2015
  • Skeletal development of chicken has been widely discussed in industrial forums and various research reports. However, these studies were emphasis on the commercial chicken strains for improve egg and meat production whereas the skeletal quiet remains as a potential weak link related to facilitating in the physical support of heavier carcasses at ever younger ages. For that, the study of standardization of skeletal development is important but it was rarely reported in Korean native chicken (KNC). The study was investigated the skeletal characteristics of KNC for international standardization. We studied in KNC at 2, 14, 28, 42, 56, 70, 84, 98, 112, 126, 147, 168, 196, 224, 336 and 448 days after hatch (male and female, n=13 for each group). We measured the body weight (BW), and after sacrifice measured organs and remove muscle from femur & tibia and measured bone weight. Data were analyzed by ANOVA, Duncan test, correlation analysis and regression analysis of SAS 9.1. We analyzed the data of BW, femur & tibia and made growth curve also. The BW was significantly increased up to 147 days after hatch (male, $1,927.88{\pm}68.92g$; female, $1,456.00{\pm}50.11g$), and then increased gradually. At 336 days, these growth was stop (male, $2,467.00{\pm}42.84g$; female, $1,568.71{\pm}62.62g$). The growth of femur & tibia length and width was stop on 98~126 days after hatch. At 98 days, we measured the length and width of femur & tibia in male were $132.39{\pm}3.18mm$ & $25.98{\pm}0.59mm$ whereas in female at 112 days the length of femur & tibia was $116.40{\pm}1.55mm$ and at 126 days width was $21.41{\pm}0.38mm$. Our study suggests that the growth of male KNC was classified pre-puberty (0~98 days), puberty (98~336 days) and maturity (after 336 days), meanwhile female was shown similar trend however puberty period of KNC was 112 or 126 days after hatch.

Analysis of the Chest Wall Reconstruction Methods after Malignant Tumor Resection

  • Gang Yeon Jo;Sae Hwi Ki
    • Archives of Plastic Surgery
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    • v.50 no.1
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    • pp.10-16
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    • 2023
  • Background The chest wall defects can be caused by various reasons. In the case of malignant tumor resection of the chest wall, it is essential to reconstruct the chest wall to cover the vital tissue and restore the pulmonary function with prevention of paradoxical motion. With our experience, we analyzed and evaluated the results and complications of the chest wall reconstructions followed by malignant tumor resection. Methods From 2013 to 2022, we reviewed a medical record of patients who received chest reconstruction due to chest wall malignant tumor resection. The following data were retrieved: patients' demographic data, tumor type, type of operation, method of chest wall reconstruction of the soft and skeletal tissue and complications. Results There were seven males and six female patients. The causes of reconstruction were 12 primary tumors and one metastatic carcinoma. The pathological types were seven sarcomas, three invasive breast carcinoma, and three squamous cell carcinomas. The skeletal reconstruction was performed in six patients. The series of the flap were eight pedicled latissimus dorsi (LD) myocutaneous flaps, two pectoralis major myocutaneous flap, two vertical rectus abdominis myocutaneous free flap, and one LD free flap. Among all the cases, only one staged reconstruction and successful reconstruction without flail chest. Most of the complications were atelectasis. Conclusion In the case of accompanying multiple ribs and sternal defect, skeletal reconstruction would need skeletal reconstruction to prevent paradoxical chest wall motion. The flap for soft tissue defect be selected according to defect size and location of chest wall. With our experience, we recommend the reconstruction algorithm for chest wall defect due to malignant tumor resection.