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Isolation and Characterization of Colletotrichum Isolates Causing Anthracnose of Japanese Plum Fruit (자두 탄저병균의 분리 및 동정)

  • Lee, Yong-Se;Ha, Da-Hee;Lee, Tae-Yi;Park, Min-Jung;Chung, Jong-Bae;Jeong, Byeong-Ryong
    • Korean Journal of Environmental Agriculture
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    • v.36 no.4
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    • pp.299-305
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    • 2017
  • BACKGROUND: Although the filamentous fungal pathogen Colletotrichum species causing anthracnose disease on various fruits including peach, apple, persimmon and grape, there is no report on Japanese plum in Korea. METHODS AND RESULTS: In 2016, diseased fruits showing typical anthracnose symptoms of Japanese plum were collected in market and ochards. Diseased tissue was cut off and disinfected subsequently with 70% ethanol for 1 min, and in 1% sodium hypochloride solution for 1 min, followed by three washes with sterile distilled water. The disinfected tissues were placed onto potato dextrose agar (PDA), and incubated at $25^{\circ}C$ in the dark for 5 to 7 days. For single-spore isolation, conidia were scraped off the plate using a loop, and suspended with 10 mL sterile distilled water. One hundred microliter of the conidial suspension was spread on PDA plates and incubated at $25^{\circ}C$. Finally, one germinated conidium was transferred onto PDA plates. Morphological and cultural characteries of colonies and spores of isolated Colletotrichum were observed after 7 to 10 days incubation on PDA. Molecular identification of isolates were analyzed by comparing rDNA-ITS gene sequences with NCBI GeneBank. CONCLUSION: Of eleven isolates of Colletotrichum isolated from anthracnose diseased Japanese plum fruits, six were identified as C. acutatum, and five as C. gloeosporioides based on diagnostic characteristics such as colony growth rate, shape and size of conidia, and rDNA-ITS sequences. This is the first report of Colletotrichum causing the anthracnose on Japanese plum in Korea.

Comparison of finite element analysis of the closing patterns between first and second premolar extraction spaces (상악 제1 및 제2소구치의 발치공간 폐쇄기전에 대한 3차원 유한요소 해석의 비교 연구)

  • Koh, Shin-Ae;Im, Won-Hee;Park, Sun-Hyung;Chun, Youn-Sic
    • The korean journal of orthodontics
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    • v.37 no.6
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    • pp.407-420
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    • 2007
  • The aim of this study was to compare the differences in closing extraction spaces between maxillary first premolar and second premolar extractions using 3-dimensional finite element analysis (FEA). Methods: Maxillary artificial teeth were selected according to Wheeler's dental anatomy. The size and shape of each tooth, bracket and archwire were made from captured real images by a 3D laser scanner and FEA was performed with a 10-noded tetrahedron. A $10^{\circ}$ gable bend was placed behind the bull loop on a $0.017"{\times}0.025"$ archwire. The extraction space was then closed through 12 repeated activating processes for each 2mm of space. Results and Conclusions: The study demonstrated that the retraction of anterior teeth was less for the second premolar extraction than for the first premolar extraction. The anterior teeth showed a controlled tipping movement with slight extrusion, and the posterior teeth showed a mesial-in rotational movement. For the second premolar extraction, buccal movement of posterior teeth was highly increased.

Long Term Variations and Environment Factors of Zooplankton Community in Lake Soyang (소양호 동물플랑크톤 군집의 장기변동과 환경요인: 2003~2014)

  • Kim, Moon Sook;Kim, Bomchul;Jun, Man-Sig
    • Korean Journal of Ecology and Environment
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    • v.51 no.1
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    • pp.29-39
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    • 2018
  • Long-term variation of zooplankton community and species composition was studied from 2003 to 2014 in a deep reservoir, Lake Soyang, in monsoon climate region, Korea. In addition, we examined the correlation with environmental factors. Annual precipitation of watershed showed a large variation in the $705{\sim}1,779mm\;yr^{-1}$ and more than 70% of it was being concentrated from June to September. The water quality of Lake Soyang was shown a clearly seasonal variations and particularly turbid water flowing into the lake during rainy season was the most important environmental factors. Zooplankton community in Lake Soyang showed a significant difference before and after 2006. Zooplankton biomass has shown a large increase and also showed a change in the zooplankton community structure since 2006. The of zooplankton showed positive correlation with temperature and BOD, Chl. a, TP concentration. These results are considered that nutrient and organic matter contained in the turbid water influences the increase in zooplankton biomass and species composition. However, water quality was limited to account for the increase in biomass of zooplankton. For example, increase of small zooplankton density (rotifer; Keratella cochlearis, Polyarthra vulgaris) in spring which is dominated by diatoms (large size; Melosira, Synedra etc.) is considered as a bottom-up effect by the microbial loop. And increased density of crustaceans in autumn was considered a top-down effects by the relationship between predator and prey of microzooplankton and mesozooplankton. In other words the inflow of allochthonous organic matter during rainy season also affected to zooplankton food web in Lake Soyang. In conclusion, biomass, diversity and long-term variations of zooplankton in Lake Soyang were determined by physico-chemical factors but also it is considered that biological interactions is important.

Study on Characteristics of Solubilization for Sewage Sludge Using Electronic Field and Ultrasonification (전기장과 초음파를 이용한 하수슬러지의 가용화 특성 연구)

  • Seo, Jang-Won;Han, Ji-Sun;Ahn, Chang-Min;Min, Dong-Hee;Yoo, Yeon-Sun;Yoon, Soon-Uk;Lee, Jong-Gyu;Lee, Jong-Yeon;Kim, Chang-Gyun
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.9
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    • pp.636-643
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    • 2011
  • Sludge solubilization using sonification has been increasingly used for sludge volume reduction along with enhancing digestion efficiency during anaerobic biogas production. In this study, either electric field or ultrasonification or in combination with were investigated using three types of sludge (return, excess and mixed at G sewage treatment facility) for the most efficient solubilization. As a the closed loop, 200 L of sludge was continuously passing through the solubilization system at an average flow rate of $0.7m^3/h$, which is equivalent to 3.5 times treated per hour for up to 84 times (24 h). Only implying electric field showed no variation for sCOD/tCOD before and after treatment on sludge solubilization regardless of types of sludge. However, employing the ultrasonic or combined system could both increasingly solubilize sludge with regard to the number of passing-through, which more enhanced by the combined. In addition, VSS/TSS was lowered to in the range of 2 and 6% while its particle size, diameter (0.9) and diameter (0.5) were more minimized than that of raw sludge. For return sludge, ultrasonification was more efficiently facilitated for solubilization, whereas electric field-ultrasonification was more preferably applied for excess and mixed sludge. It is concluded that depending on types of sludge, solubilization system must be selectively applied for the most efficient break-up of them.

Thermal Performance of a Heating Board with a Microencapsulated Myristic Acid (Myristic acid를 사용한 축열장판의 열성능 연구)

  • Kwak, H.Y.;Kang, Y.H.;Jeon, M.S.;Lee, T.K.;Lee, D.G.;Han, G.Y.
    • Solar Energy
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    • v.18 no.3
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    • pp.177-183
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    • 1998
  • The thermal performance of a heating board with microencapsualted PCM was investigated and compared to conventional heating board. The employed PCM was the myristic acid $$ and was encapsulated by the multiple layers of PMMA and paraffin wax. The size of encapsulated PCM was $1{\sim}1.5mm$. Accoring to ANSI/ASHRAE test procedure, the close-loop test configuration was installed. Air was used as the heat transfer fluid and a calibrated orifice was employed for the measurement of air flow rates. The thermal performance test of two different heating boards(with 10 wt% PCM and without PCM) was conducted for different air flow rates and the heat transfer characteristics during cooling was compared. The test results showed that the surface temperature of heating board with 10 wt% PCM maintained higher during the cooling process than that of the heating board without PCM and experimentally determined heat transfer coefficient in heating board with PCM showed higher value compared to heating board without PCM.

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Improvement of Performance Test Standards for Marine Pollution Prevention Materials and Chemicals (for Eco-toxicity Test) (해양오염방제 자재·약제의 성능시험기준 개선방안에 관한 연구(생태독성시험 항목))

  • Kim, Tae Won;Lee, Su Jin;Kim, Young Ryun;Lee, Moon Jin
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.6
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    • pp.744-753
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    • 2021
  • This study suggests ways to improve the standard test method and judgment criterion for the "Eco-toxicity Test" based on the rules and regulations provided in 'performance and qualifying test standards for marine pollution prevention materials and chemicals' in the Republic of Korea. Compared with the relevant references of other countries, this study attempted to find the limitations in the existing standards. As for the growth inhibition test of algae using Skeletonema costatum as an indicator, applying comparative analysis to measure specific growth rates, together with statistical analysis, instead of applying current methods, judged by the appearance of colors from the culture medium was suggested. Considering the exponential growth phase of the test species, the test duration was suggested to be reduced to less than four days. For the test with fish as an indicator, resetting the appropriate body size was suggested to, show consistent sensitivity against chenicals applied during testing. Furthermore, it is necessary to consider the criteria needs, which should be established in reasonable and objective ways. For the testing species, marine rotifer, Brachionus plicatilis could be a replacement for Artemia sp., and a bivalve for fish in the test. To improve the performance effectiveness of the "Eco-toxicity test", it is worth considering the designation of accredited testing institutes, by placing it in the same loop. Thus it is also expected to have a reliable management system in place, having the capacity of cost calculation properly and adjusting testing items if required.

Formation of a large-scale quasi-circular flare ribbon enclosing three-ribbon through two-step eruptive flares

  • Lim, Eun-Kyung;Yurchyshyn, Vasyl;Kumar, Pankaj;Cho, Kyuhyoun;Kim, Sujin;Cho, Kyung-Suk
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.2
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    • pp.42.1-42.1
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    • 2016
  • The formation process and the dynamical properties of a large-scale quasi-circular flare ribbon were investigated using the SDO AIA and HMI data along with data from RHESSI and SOT. Within one hour time interval, two subsequent M-class flares were detected from the NOAA 12371 that had a ${\beta}{\gamma}{\delta}$ configuration with one bipolar sunspot group in the east and one unipolar spot in the west embedded in a decayed magnetic field. Earlier M2.0 flare was associated with a coronal loop eruption, and a two-ribbon structure formed within the bipolar sunspot group. On the other hand, the later M2.6 flare was associated with a halo CME, and a quasi-circular ribbon developed encircling the full active region. The observed quasi-circular ribbon was strikingly large in size spanning 650" in north-south and 500" in east-west direction. It showed the well-known sequential brightening in the clockwise direction during the decay phase of the M2.6 flare at the estimated speed of 160.7 km s-1. The quasi-circular ribbon also showed the radial expansion, especially in the southern part. Interestingly, at the time of the later M2.6 flare, the third flare ribbon parallel to the early two-ribbon structure also developed near the unipolar sunspot, then showed a typical separation in pair with the eastern most ribbon of the early two ribbons. The potential field reconstruction based on the PFSS model showed a fan shaped magnetic configuration including fan-like field lines stemming from the unipolar spot and fanning out toward the background decayed field. This large-scale fan-like field overarched full active region, and the footpoints of fan-like field lines were co-spatial with the observed quasi-circular ribbon. From the NLFF magnetic field reconstruction, we confirmed the existence of a twisted flux rope structure in the bipolar spot group before the first M2.0 flare. Hard X-ray emission signatures were detected at the site of twisted flux rope during the pre-flare phase of the M2.0 flare. Based on the analysis of both two-ribbon structure and quasi-circular ribbon, we suggest that a tether-cutting reconnection between sheared arcade overarching the twisted flux rope embedded in a fan-like magnetic field may have triggered the first M2.0 flare, then secondary M2.6 flare was introduced by the fan-spine reconnection because of the interaction between the expanding field and the nearby quasi-null and formed the observed large-scale quasi-circular flare ribbon.

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A Study on the Utilization and Control Method of Hybrid Switching Tap Based Automatic Voltage Regulator on Smart Grid (스마트그리드의 탭 전환 자동 전압 조정기의 다중 스위칭 제어 방법 및 활용 방안에 관한 연구)

  • Park, Gwang-Yun;Kim, Jung-Ryul;Kim, Byung-Gi
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.12
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    • pp.31-39
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    • 2012
  • In this paper, we propose a microprocessor-based automatic voltage regulator(AVR) to reduce consumers' electric energy consumption and to help controlling peak demanding power. Hybrid Switching Automatic Voltage Regulator (HS-AVR) consist of a toroidal core, several tap control switches, display and command control parts. The coil forms an autotransformer which has a serial main winding and four parallel auxiliary windings. It controls the output voltage by changing the combination of the coils and the switches. Relays are adopted as the link switches of the coils to minimize the loss. To make connecting and disconnecting time accurate, relays of the circuit have parallel TRIACs. A software phase locked loop(PLL) has been used to synchronize the timings of the switches to the voltage waveform. The software PLL informs the input voltage zero-crossing and positive/negative peak timing. The traditional voltage transformers and AVRs have a disadvantage of having a large mandatory capacity to accommodate maximum inrush current to avoid the switch contact damage. But we propose a suitable AVR for every purpose in smart grid with reduced size and increased efficiency.

Design of Video Encoder activating with variable clocks of CCDs for CCTV applications (CCTV용 CCD를 위한 가변 clock으로 동작되는 비디오 인코더의 설계)

  • Kim, Joo-Hyun;Ha, Joo-Young;Kang, Bong-Soon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.1
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    • pp.80-87
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    • 2006
  • SONY corporation preoccupies $80\%$ of a market of the CCD used in a CCTV system. The CCD of SONY have high duality which can not follow the progress of capability. But there are some problems which differ the clock frequency used in CCD from the frequency used in common video encoder. To get the result by using common video encoder, the system needs a scaler that could adjust image size and PLL that synchronizes CCD's with encoder's clock So, this paper proposes the video encoder that is activated at equal clock used in CCD without scaler and PLL. The encoder converts ITU-R BT.601 4:2:2 or ITU-R BT.656 inputs from various video sources into NTSC or PAL signals in CVBS. Due to variable clock, property of filters used in the encoder is automatically changed by clock and filters adopt multiplier-free structures to reduce hardware complexity. The hardware bit width of programmable digital filters for luminance and chrominance signals, along with other operating blocks, are carefully determined to produce hish-quality digital video signals of ${\pm}1$ LSB error or less. The proposed encoder is experimentally demonstrated by using the Altera Stratix EP1S80B953C6ES device.

Accelerated Convolution Image Processing by Using Look-Up Table and Overlap Region Buffering Method (Loop-Up Table과 필터 중첩영역 버퍼링 기법을 이용한 컨벌루션 영상처리 고속화)

  • Kim, Hyun-Woo;Kim, Min-Young
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.49 no.4
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    • pp.17-22
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    • 2012
  • Convolution filtering methods have been widely applied to various digital signal processing fields for image blurring, sharpening, edge detection, and noise reduction, etc. According to their application purpose, the filter mask size or shape and the mask value are selected in advance, and the designed filter is applied to input image for the convolution processing. In this paper, we proposed an image processing acceleration method for the convolution processing by using two-dimensional Look-up table (LUT) and overlap-region buffering technique. First, based on the fixed convolution mask value, the multiplication operation between 8 or 10 bit pixel values of the input image and the filter mask values is performed a priori, and the results memorized in LUT are referred during the convolution process. Second, based on symmetric structural characteristics of the convolution filters, inherent duplicated operation region is analysed, and the saved operation results in one step before in the predefined memory buffer is recalled and reused in current operation step. Through this buffering, unnecessary repeated filter operation on the same regions is minimized in sequential manner. As the proposed algorithms minimize the computational amount needed for the convolution operation, they work well under the operation environments utilizing embedded systems with limited computational resources or the environments of utilizing general personnel computers. A series of experiments under various situations verifies the effectiveness and usefulness of the proposed methods.